GH719935

EST Overview


Name	GH719935
Unique Name	GH719935
Type	EST
Organism	Pisum sativum (pea)
Sequence length	482

Libraries


Library Name	Type
LIBEST_024269 TNI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH719935 vs. TrEMBL
Match: B7FH05_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 261.922 bits (668), Expect = 5.786e-78
Identity = 129/139 (92.81%), Postives = 133/139 (95.68%), Query Frame = 3

Query:   66 PVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            P   +QFTEY+QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDK+ GPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRH+HSGMSVEEAIDLVDKCI EIR RLVVAPPNFVIKIVDKDGAR
Sbjct:   50 PGDRVQFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHYHSGMSVEEAIDLVDKCIAEIRSRLVVAPPNFVIKIVDKDGAR 188

HSP 2 Score: 53.5286 bits (127), Expect = 5.786e-78
Identity = 24/25 (96.00%), Postives = 25/25 (100.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            KSNEDKIMFLDSHKL+AASGEPGDR
Sbjct:   29 KSNEDKIMFLDSHKLVAASGEPGDR 53

[Close]

BLAST of GH719935 vs. TrEMBL
Match: C6SW24_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 1.284e-77
Identity = 129/139 (92.81%), Postives = 133/139 (95.68%), Query Frame = 3

Query:   66 PVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            P   +QFTEY+QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDK+ GP LYYIDYIATLHKLEKGAFGYGSYFSLSMMDRH+HSGMSVEEAIDLVDKCIMEIR RLVVAPPNFVIKIVDKDGAR
Sbjct:   50 PGDRVQFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPELYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHYHSGMSVEEAIDLVDKCIMEIRSRLVVAPPNFVIKIVDKDGAR 188

HSP 2 Score: 51.6026 bits (122), Expect = 1.284e-77
Identity = 24/25 (96.00%), Postives = 24/25 (96.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            KSNEDKIM LDSHKLIAASGEPGDR
Sbjct:   29 KSNEDKIMLLDSHKLIAASGEPGDR 53

[Close]

BLAST of GH719935 vs. TrEMBL
Match: C6SY85_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 1.674e-77
Identity = 128/139 (92.09%), Postives = 133/139 (95.68%), Query Frame = 3

Query:   66 PVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            P   +QFTEY+QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDK+ GP LYYIDYIATLHKLEKGAFGYGSYFSLSMMDRH+HSGMSVEEA+DLVDKCIMEIR RLVVAPPNFVIKIVDKDGAR
Sbjct:   50 PGDRVQFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPELYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHYHSGMSVEEAVDLVDKCIMEIRSRLVVAPPNFVIKIVDKDGAR 188

HSP 2 Score: 51.6026 bits (122), Expect = 1.674e-77
Identity = 24/25 (96.00%), Postives = 24/25 (96.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            KSNEDKIM LDSHKLIAASGEPGDR
Sbjct:   29 KSNEDKIMLLDSHKLIAASGEPGDR 53

[Close]

BLAST of GH719935 vs. TrEMBL
Match: B9RTN1_RICCO (Proteasome subunit beta type OS=Ricinus communis GN=RCOM_0911560 PE=3 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 4.059e-76
Identity = 125/139 (89.93%), Postives = 134/139 (96.40%), Query Frame = 3

Query:   66 PVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            P   +QFTEY+QKNV+LYQFRNGIPL+TAAAANFTRGELATALRKNPYSVNILLAGYDK+ GPSLYYIDYIATLHK++KGAFGYGSYFSL+MMDRHFHSGMSVEEAIDLVDKCI+EIR RLVVAPPNFVIKIVDKDGAR
Sbjct:   50 PGDRVQFTEYIQKNVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYIATLHKVDKGAFGYGSYFSLAMMDRHFHSGMSVEEAIDLVDKCILEIRSRLVVAPPNFVIKIVDKDGAR 188

HSP 2 Score: 51.2174 bits (121), Expect = 4.059e-76
Identity = 24/25 (96.00%), Postives = 24/25 (96.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            KSNEDKIM LDSHKLIAASGEPGDR
Sbjct:   29 KSNEDKIMVLDSHKLIAASGEPGDR 53

[Close]

BLAST of GH719935 vs. TrEMBL
Match: A5BYC0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006154 PE=4 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 5.781e-75
Identity = 125/134 (93.28%), Postives = 133/134 (99.25%), Query Frame = 3

Query:   81 QFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            QFTEY+QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPY+VNILLAGYDKDIGPSLYYIDYIA+LHK+EKGAFGYGSYFSL+MMDRH+HSGM+VEEA+DLVDKCIMEIR RLVVAPPNFVIKIVDKDGAR
Sbjct:   55 QFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYAVNILLAGYDKDIGPSLYYIDYIASLHKIEKGAFGYGSYFSLAMMDRHYHSGMTVEEAVDLVDKCIMEIRSRLVVAPPNFVIKIVDKDGAR 188

HSP 2 Score: 45.4394 bits (106), Expect = 5.781e-75
Identity = 21/25 (84.00%), Postives = 22/25 (88.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            KSNEDKIM LDSHKL+ ASGE GDR
Sbjct:   29 KSNEDKIMVLDSHKLLGASGEGGDR 53

[Close]

BLAST of GH719935 vs. TrEMBL
Match: B9HUT6_POPTR (Proteasome subunit beta type OS=Populus trichocarpa GN=POPTRDRAFT_814139 PE=3 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 5.768e-72
Identity = 119/135 (88.15%), Postives = 130/135 (96.30%), Query Frame = 3

Query:   78 IQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            +QFTEY+QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPY VNILLAGYDK+ GPSLYYIDYIATLHK+++GAFGYGSYF LSMMDRH+HSGMSVEEA++LVDKCI EI+ RLVVAPPNFVIKIVD+DGAR
Sbjct:   54 VQFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYMVNILLAGYDKETGPSLYYIDYIATLHKVDRGAFGYGSYFCLSMMDRHYHSGMSVEEAVELVDKCITEIQSRLVVAPPNFVIKIVDRDGAR 188

HSP 2 Score: 45.8246 bits (107), Expect = 5.768e-72
Identity = 21/25 (84.00%), Postives = 23/25 (92.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            K+NEDKIM LDSHKL+AASGE GDR
Sbjct:   29 KTNEDKIMKLDSHKLVAASGESGDR 53

[Close]

BLAST of GH719935 vs. TrEMBL
Match: A9PIV5_9ROSI (Proteasome subunit beta type OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 5.768e-72
Identity = 119/135 (88.15%), Postives = 130/135 (96.30%), Query Frame = 3

Query:   78 IQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            +QFTEY+QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPY VNILLAGYDK+ GPSLYYIDYIATLHK+++GAFGYGSYF LSMMDRH+HSGMSVEEA++LVDKCI EI+ RLVVAPPNFVIKIVD+DGAR
Sbjct:   54 VQFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYMVNILLAGYDKETGPSLYYIDYIATLHKVDRGAFGYGSYFCLSMMDRHYHSGMSVEEAVELVDKCITEIQSRLVVAPPNFVIKIVDRDGAR 188

HSP 2 Score: 45.8246 bits (107), Expect = 5.768e-72
Identity = 21/25 (84.00%), Postives = 23/25 (92.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            K+NEDKIM LDSHKL+AASGE GDR
Sbjct:   29 KTNEDKIMKLDSHKLVAASGESGDR 53

[Close]

BLAST of GH719935 vs. TrEMBL
Match: D7L1R8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_898737 PE=4 SV=1)

HSP 1 Score: 242.662 bits (618), Expect = 4.829e-71
Identity = 119/139 (85.61%), Postives = 129/139 (92.81%), Query Frame = 3

Query:   66 PVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            P   +QFTEYVQKNV+LY+FRNGIPLTTAAAANFTRGELATALRKNPYSVNIL+AGYD + G SLYYIDYIATLHK++KGAFGYGSYFSLS MDRH+ S MSVEEAI+LVDKCI+EIR RLVVAPPNFVIKIVDKDGAR
Sbjct:   50 PGDRVQFTEYVQKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDNESGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVVAPPNFVIKIVDKDGAR 188

HSP 2 Score: 49.6766 bits (117), Expect = 4.829e-71
Identity = 22/25 (88.00%), Postives = 24/25 (96.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            K+NEDKIM LDSHKL+AASGEPGDR
Sbjct:   29 KNNEDKIMVLDSHKLVAASGEPGDR 53

[Close]

BLAST of GH719935 vs. TrEMBL
Match: D7TDZ5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_151.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001182001 PE=4 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 6.316e-71
Identity = 125/134 (93.28%), Postives = 133/134 (99.25%), Query Frame = 3

Query:   81 QFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            QFTEY+QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPY+VNILLAGYDKDIGPSLYYIDYIA+LHK+EKGAFGYGSYFSL+MMDRH+HSGM+VEEA+DLVDKCIMEIR RLVVAPPNFVIKIVDKDGAR
Sbjct:   20 QFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYAVNILLAGYDKDIGPSLYYIDYIASLHKIEKGAFGYGSYFSLAMMDRHYHSGMTVEEAVDLVDKCIMEIRSRLVVAPPNFVIKIVDKDGAR 153

HSP 2 Score: 31.9574 bits (71), Expect = 6.316e-71
Identity = 14/18 (77.78%), Postives = 15/18 (83.33%), Query Frame = 2

Query:   23 MFLDSHKLIAASGEPGDR 76
            M LDSHKL+ ASGE GDR
Sbjct:    1 MVLDSHKLLGASGEGGDR 18

[Close]

BLAST of GH719935 vs. TrEMBL
Match: A9NKA9_PICSI (Proteasome subunit beta type OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 1.275e-68
Identity = 113/135 (83.70%), Postives = 128/135 (94.81%), Query Frame = 3

Query:   78 IQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            +QFTEY+QKNV LYQFRNGIPL+TAA ANFTRGELATALRKNPYSVN+LLAG+DKD+GPSLYYIDYIATLHK++K AFGYG+YF+LSMMDR++H GMSVEEAI+  DKCI+EIR RL+VAPPNFVIKIVDKDGAR
Sbjct:   54 VQFTEYIQKNVHLYQFRNGIPLSTAATANFTRGELATALRKNPYSVNLLLAGFDKDVGPSLYYIDYIATLHKVDKVAFGYGAYFALSMMDRYYHKGMSVEEAIEFADKCILEIRSRLIVAPPNFVIKIVDKDGAR 188

HSP 2 Score: 43.1282 bits (100), Expect = 1.275e-68
Identity = 19/25 (76.00%), Postives = 22/25 (88.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            K++EDKIM LDSHKL+ ASGE GDR
Sbjct:   29 KTSEDKIMILDSHKLLGASGESGDR 53

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2A_ARATH (Proteasome subunit beta type-2-A OS=Arabidopsis thaliana GN=PBD1 PE=1 SV=1)

HSP 1 Score: 242.276 bits (617), Expect = 4.421e-72
Identity = 119/139 (85.61%), Postives = 129/139 (92.81%), Query Frame = 3

Query:   66 PVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            P   +QFTEYVQKNV+LY+FRNGIPLTTAAAANFTRGELATALRKNPYSVNIL+AGYD + G SLYYIDYIATLHK++KGAFGYGSYFSLS MDRH+ S MSVEEAI+LVDKCI+EIR RLVVAPPNFVIKIVDKDGAR
Sbjct:   50 PGDRVQFTEYVQKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDDESGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVVAPPNFVIKIVDKDGAR 188

HSP 2 Score: 49.6766 bits (117), Expect = 4.421e-72
Identity = 22/25 (88.00%), Postives = 24/25 (96.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            K+NEDKIM LDSHKL+AASGEPGDR
Sbjct:   29 KNNEDKIMVLDSHKLVAASGEPGDR 53

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2_ORYSJ (Proteasome subunit beta type-2 OS=Oryza sativa subsp. japonica GN=PBD1 PE=2 SV=2)

HSP 1 Score: 225.328 bits (573), Expect = 1.266e-65
Identity = 108/139 (77.70%), Postives = 122/139 (87.77%), Query Frame = 3

Query:   66 PVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            P   +QFTE++QKN+ LYQFRN IPL+TAA ANFTRGELATALRKNPY VN+LLAGYD D+G SLYYIDYIAT HK+EKGAFGYGSYF LS+MD+ +   MSVEEA+DLVDKCI EIRLRLVVAP NF+IKIVDK+GAR
Sbjct:   50 PGDRVQFTEFIQKNLHLYQFRNNIPLSTAATANFTRGELATALRKNPYYVNVLLAGYDSDVGASLYYIDYIATFHKIEKGAFGYGSYFCLSLMDKLYRPDMSVEEAVDLVDKCIKEIRLRLVVAPQNFIIKIVDKEGAR 188

HSP 2 Score: 45.0542 bits (105), Expect = 1.266e-65
Identity = 19/25 (76.00%), Postives = 23/25 (92.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            K++EDK+M LDSHKL+ ASGEPGDR
Sbjct:   29 KTDEDKVMVLDSHKLMGASGEPGDR 53

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2B_ARATH (Proteasome subunit beta type-2-B OS=Arabidopsis thaliana GN=PBD2 PE=1 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 1.014e-64
Identity = 124/160 (77.50%), Postives = 139/160 (86.88%), Query Frame = 3

Query:   12 KTRSCSSIPINSLLLVA---NPVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            K +    + ++S  LVA    P   +QFTEYVQKNV+LYQFRNGIPL+TAAAANFTRGELATALRKNPYSVNIL+AGYDK+ G SLYYIDYIATLHK++KGAFGYGSYFSLS MDRH+ S MSVEEAI+LVDKCI+EIR RLV+APPNFVIKIVDKDGAR
Sbjct:   29 KNKEDKIMTLDSHKLVAASGEPGDRVQFTEYVQKNVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVIAPPNFVIKIVDKDGAR 188

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2_DICDI (Proteasome subunit beta type-2 OS=Dictyostelium discoideum GN=psmB2 PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 6.956e-38
Identity = 65/138 (47.10%), Postives = 96/138 (69.57%), Query Frame = 3

Query:   78 IQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDI---GPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            +QFTEY+ KN+ LY  RN  P++T AAANF R ELAT++R +PYS+N++LAGYDK++   G SLYY+DY+ +L KL  G  GY SYF L ++DRH    +S+E+ I+L+  C  E++ R +V+   + +K V  +G +
Sbjct:   54 VQFTEYISKNIKLYSLRNSYPMSTPAAANFIRNELATSIRSHPYSINLILAGYDKEVEDGGTSLYYMDYLGSLQKLNFGCHGYASYFLLGLLDRHHKCDLSLEDGINLMHLCTTELKTRFLVS-GKYTLKFVSSEGIK 190

HSP 2 Score: 35.4242 bits (80), Expect = 6.956e-38
Identity = 14/25 (56.00%), Postives = 20/25 (80.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            K +EDKI+ +D HKL+ ++GE GDR
Sbjct:   29 KDDEDKILEIDGHKLLLSAGEAGDR 53

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2_RAT (Proteasome subunit beta type-2 OS=Rattus norvegicus GN=Psmb2 PE=1 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 1.428e-34
Identity = 70/144 (48.61%), Postives = 100/144 (69.44%), Query Frame = 3

Query:   48 LLLVANPVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALR-KNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDG 476
            LL V      +QF EY+QKNV LY+ RNG  L+  AAANFTR  LA  LR + PY VN+LLAGYD+  GP+LYY+DY+A L K    A GYG++ +LS++DR++   +S E A++L+ KC+ E++ R ++  P F ++++DKDG
Sbjct:   44 LLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRVIDKDG 187

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2_MOUSE (Proteasome subunit beta type-2 OS=Mus musculus GN=Psmb2 PE=1 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 1.428e-34
Identity = 70/144 (48.61%), Postives = 100/144 (69.44%), Query Frame = 3

Query:   48 LLLVANPVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALR-KNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDG 476
            LL V      +QF EY+QKNV LY+ RNG  L+  AAANFTR  LA  LR + PY VN+LLAGYD+  GP+LYY+DY+A L K    A GYG++ +LS++DR++   +S E A++L+ KC+ E++ R ++  P F ++++DKDG
Sbjct:   44 LLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRVIDKDG 187

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2_HUMAN (Proteasome subunit beta type-2 OS=Homo sapiens GN=PSMB2 PE=1 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 4.156e-34
Identity = 70/144 (48.61%), Postives = 100/144 (69.44%), Query Frame = 3

Query:   48 LLLVANPVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALR-KNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDG 476
            LL V      +QF EY+QKNV LY+ RNG  L+  AAANFTR  LA  LR + PY VN+LLAGYD+  GP+LYY+DY+A L K    A GYG++ +LS++DR++   +S E A++L+ KC+ E++ R ++  P F ++I+DK+G
Sbjct:   44 LLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNG 187

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2_BOVIN (Proteasome subunit beta type-2 OS=Bos taurus GN=PSMB2 PE=1 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.209e-33
Identity = 69/144 (47.92%), Postives = 101/144 (70.14%), Query Frame = 3

Query:   48 LLLVANPVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALR-KNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDG 476
            LL V      +QF EY+QKNV LY+ RNG  L+  AAANFTR  LA  LR + PY VN+LLAGYD+  GP+LYY+DY+A L K    A GYG++ +LS++DR++   +S E+A++L+ KC+ E++ R ++  P F ++I+D++G
Sbjct:   44 LLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADYLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISREKAVELLRKCLEELQKRFILNLPTFSVRIIDRNG 187

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2_NEUCR (Probable proteasome subunit beta type-2 OS=Neurospora crassa GN=15E6.60 PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 1.655e-29
Identity = 61/135 (45.19%), Postives = 87/135 (64.44%), Query Frame = 3

Query:   78 IQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALR-KNPYSVNILLAGYDKDIG-PSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDG 476
            +QF EY Q N  LY  RNG  L+ +A ANF RGELAT+LR + PY+VN+LL G D     PSLY++DY+A+L K+   A GY  Y+ LS++D+H H  +++ + I L++ C  E++ RL +      +K V KDG
Sbjct:   54 VQFAEYTQANAQLYSMRNGTDLSPSALANFVRGELATSLRSRKPYNVNLLLGGVDPITHKPSLYWLDYLASLAKVPYAAHGYAQYYCLSILDKHHHPDITLHQGIKLLNLCTDELKRRLPIDFKGMTVKAVTKDG 188

HSP 2 Score: 28.1054 bits (61), Expect = 1.655e-29
Identity = 12/24 (50.00%), Postives = 17/24 (70.83%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGD 73
            K+++DK   L+ H L+A SGE GD
Sbjct:   29 KASDDKTRSLNKHTLMAFSGEAGD 52

[Close]

BLAST of GH719935 vs. SwissProt
Match: PSB2_PICGU (Probable proteasome subunit beta type-2 OS=Pichia guilliermondii GN=PRO2 PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 2.157e-29
Identity = 60/138 (43.48%), Postives = 89/138 (64.49%), Query Frame = 3

Query:   78 IQFTEYVQKNVALYQFR-NGIPLTTAAAANFTRGELATALR-KNPYSVNILLAGYDKDIG-PSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            +QF EY+Q N  LY  R N   L+  A A+F R +LAT++R + PY VN+LLAGYD + G PSL +IDY+ T  +L   A GY  ++  S++D+H+  GM+ E+ +DL+ KCI E+  R+ +      IK+VDK+G +
Sbjct:   54 VQFAEYIQANTQLYTMRENDTELSPKATASFVRNQLATSIRSRKPYQVNVLLAGYDTNTGKPSLNWIDYLGTQVELPYAAHGYAGFYCTSLLDKHYKKGMNFEDGLDLLKKCIKELETRMPIDFKGCYIKVVDKEGIK 191

HSP 2 Score: 28.8758 bits (63), Expect = 2.157e-29
Identity = 13/24 (54.17%), Postives = 16/24 (66.67%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGD 73
            K  +DK   L+SH L+A SGE GD
Sbjct:   29 KDTDDKTRQLNSHNLMAYSGEAGD 52

[Close]

BLAST of GH719935 vs. TAIR peptide
Match: AT3G22630.1 (| Symbols: PBD1, PRCGB | 20S proteasome beta subunit D1 | chr3:8009709-8010774 REVERSE LENGTH=204)

HSP 1 Score: 242.276 bits (617), Expect = 5.092e-73
Identity = 119/139 (85.61%), Postives = 129/139 (92.81%), Query Frame = 3

Query:   66 PVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            P   +QFTEYVQKNV+LY+FRNGIPLTTAAAANFTRGELATALRKNPYSVNIL+AGYD + G SLYYIDYIATLHK++KGAFGYGSYFSLS MDRH+ S MSVEEAI+LVDKCI+EIR RLVVAPPNFVIKIVDKDGAR
Sbjct:   50 PGDRVQFTEYVQKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDDESGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVVAPPNFVIKIVDKDGAR 188

HSP 2 Score: 49.6766 bits (117), Expect = 5.092e-73
Identity = 22/25 (88.00%), Postives = 24/25 (96.00%), Query Frame = 2

Query:    2 KSNEDKIMFLDSHKLIAASGEPGDR 76
            K+NEDKIM LDSHKL+AASGEPGDR
Sbjct:   29 KNNEDKIMVLDSHKLVAASGEPGDR 53

[Close]

BLAST of GH719935 vs. TAIR peptide
Match: AT4G14800.1 (| Symbols: PBD2 | 20S proteasome beta subunit D2 | chr4:8500398-8502058 FORWARD LENGTH=199)

HSP 1 Score: 245.358 bits (625), Expect = 1.171e-65
Identity = 124/160 (77.50%), Postives = 139/160 (86.88%), Query Frame = 3

Query:   12 KTRSCSSIPINSLLLVA---NPVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            K +    + ++S  LVA    P   +QFTEYVQKNV+LYQFRNGIPL+TAAAANFTRGELATALRKNPYSVNIL+AGYDK+ G SLYYIDYIATLHK++KGAFGYGSYFSLS MDRH+ S MSVEEAI+LVDKCI+EIR RLV+APPNFVIKIVDKDGAR
Sbjct:   29 KNKEDKIMTLDSHKLVAASGEPGDRVQFTEYVQKNVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVIAPPNFVIKIVDKDGAR 188

[Close]

BLAST of GH719935 vs. TAIR peptide
Match: AT4G14800.2 (| Symbols: PBD2 | 20S proteasome beta subunit D2 | chr4:8500398-8502058 FORWARD LENGTH=215)

HSP 1 Score: 234.958 bits (598), Expect = 1.582e-62
Identity = 124/176 (70.45%), Postives = 139/176 (78.98%), Query Frame = 3

Query:   12 KTRSCSSIPINSLLLVA---NPVTGIQFTEYVQKNVALYQFRNGIPLTTAAAANFTRGELATALRK----------------NPYSVNILLAGYDKDIGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMSVEEAIDLVDKCIMEIRLRLVVAPPNFVIKIVDKDGAR 482
            K +    + ++S  LVA    P   +QFTEYVQKNV+LYQFRNGIPL+TAAAANFTRGELATALRK                NPYSVNIL+AGYDK+ G SLYYIDYIATLHK++KGAFGYGSYFSLS MDRH+ S MSVEEAI+LVDKCI+EIR RLV+APPNFVIKIVDKDGAR
Sbjct:   29 KNKEDKIMTLDSHKLVAASGEPGDRVQFTEYVQKNVSLYQFRNGIPLSTAAAANFTRGELATALRKEFCATFQGYSDFCVLMNPYSVNILMAGYDKEAGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVIAPPNFVIKIVDKDGAR 204

The following BLAST results are available for this feature:

BLAST of GH719935 vs. TrEMBL
BLAST of GH719935 vs. SwissProt
BLAST of GH719935 vs. TAIR peptide

BLAST of GH719935 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FH05_MEDTR	5.786e-78	92.81	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6SW24_SOYBN	1.284e-77	92.81	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SY85_SOYBN	1.674e-77	92.09	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9RTN1_RICCO	4.059e-76	89.93	Proteasome subunit beta type OS=Ricinus communis G...	[more]
A5BYC0_VITVI	5.781e-75	93.28	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9HUT6_POPTR	5.768e-72	88.15	Proteasome subunit beta type OS=Populus trichocarp...	[more]
A9PIV5_9ROSI	5.768e-72	88.15	Proteasome subunit beta type OS=Populus trichocarp...	[more]
D7L1R8_ARALY	4.829e-71	85.61	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7TDZ5_VITVI	6.316e-71	93.28	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9NKA9_PICSI	1.275e-68	83.70	Proteasome subunit beta type OS=Picea sitchensis P...	[more]

BLAST of GH719935 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PSB2A_ARATH	4.421e-72	85.61	Proteasome subunit beta type-2-A OS=Arabidopsis th...	[more]
PSB2_ORYSJ	1.266e-65	77.70	Proteasome subunit beta type-2 OS=Oryza sativa sub...	[more]
PSB2B_ARATH	1.014e-64	77.50	Proteasome subunit beta type-2-B OS=Arabidopsis th...	[more]
PSB2_DICDI	6.956e-38	47.10	Proteasome subunit beta type-2 OS=Dictyostelium di...	[more]
PSB2_RAT	1.428e-34	48.61	Proteasome subunit beta type-2 OS=Rattus norvegicu...	[more]
PSB2_MOUSE	1.428e-34	48.61	Proteasome subunit beta type-2 OS=Mus musculus GN=...	[more]
PSB2_HUMAN	4.156e-34	48.61	Proteasome subunit beta type-2 OS=Homo sapiens GN=...	[more]
PSB2_BOVIN	1.209e-33	47.92	Proteasome subunit beta type-2 OS=Bos taurus GN=PS...	[more]
PSB2_NEUCR	1.655e-29	45.19	Probable proteasome subunit beta type-2 OS=Neurosp...	[more]
PSB2_PICGU	2.157e-29	43.48	Probable proteasome subunit beta type-2 OS=Pichia ...	[more]

BLAST of GH719935 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT3G22630.1	5.092e-73	85.61	\| Symbols: PBD1, PRCGB \| 20S proteasome beta subun...	[more]
AT4G14800.1	1.171e-65	77.50	\| Symbols: PBD2 \| 20S proteasome beta subunit D2 \|...	[more]
AT4G14800.2	1.582e-62	70.45	\| Symbols: PBD2 \| 20S proteasome beta subunit D2 \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001353	Proteasome, subunit alpha/beta	PFAM	PF00227	Proteasome	coord: 27..154 score: 1.1
None	No IPR available	GENE3D	3.60.20.10	no description	coord: 27..159 score: 4.9
None	No IPR available	PANTHER	PTHR11599	PROTEASOME SUBUNIT ALPHA/BETA	coord: 26..160 score: 1.4
None	No IPR available	PANTHER	PTHR11599:SF6	PROTEASOME SUBUNIT BETA TYPE 2	coord: 26..160 score: 1.4
None	No IPR available	PROFILE	PS51476	PROTEASOME_B_2	coord: 27..154 score: 31
None	No IPR available	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 27..158 score: 3.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH719935 ID=GH719935; Name=GH719935; organism=Pisum sativum; type=EST; length=482bp
CAAATCCAATGAAGACAAGATCATGTTCCTCGATTCCCATAAACTCATTG
CTGCTAGTGGCGAACCCGGTGACAGGGATTCAGTTTACTGAGTATGTTCA
AAAGAACGTTGCTTTGTATCAGTTCCGTAATGGGATCCCTCTCACCACTG
CCGCCGCCGCTAATTTCACTCGTGGCGAGCTCGCCACCGCTCTCCGCAAG
AACCCTTACTCTGTGAACATCCTTCTTGCTGGTTATGACAAAGATATAGG
CCCATCACTATACTACATCGACTACATTGCAACACTTCACAAGCTCGAAA
AGGGAGCATTTGGTTACGGTTCCTATTTCTCACTGTCAATGATGGACAGA
CACTTCCATAGCGGAATGTCCGTGGAAGAAGCAATCGATCTTGTTGATAA
GTGCATTATGGAAATCAGATTAAGGTTGGTTGTCGCACCTCCAAACTTTG
TTATCAAGATTGTTGACAAGGATGGTGCAAGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004298	threonine-type endopeptidase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005839	proteasome core complex

Vocabulary: **Biological Process**
Term	Definition
GO:0051603	proteolysis involved in protein catabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR001353	Proteasome_sua/b

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