AM162056

EST Overview
NameAM162056
Unique NameAM162056
TypeEST
OrganismPisum sativum (pea)
Sequence length419
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
InterProScan analysis for Pisum sativum unigene v12010-12-29
Homology
[Close]
BLAST of AM162056 vs. TrEMBL
Match: B9I395_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_891283 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 6.172e-12
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 2 
Query:   14 GKSPGTRCCVC*LQT*SDAYLSLARYDEAVFSYQKALTVFKSTKGENHTNSCLCFCKVSRFIQQNRKVQ 220
            G+     C  C +    DAYLSL+RYDEA+F+YQKALT FK+TKGENH +    F +++    +  K++
Sbjct:  380 GQEAEVACVDCSI---GDAYLSLSRYDEAIFAYQKALTAFKTTKGENHPSVASVFVRLAHLCNRTGKLR 445

HSP 2 Score: 30.0314 bits (66), Expect = 6.172e-12
Identity = 16/35 (45.71%), Postives = 23/35 (65.71%), Query Frame = 1 
Query:  220 RNLKLIAKIALRIFGKKNPGIPLEEIANGLIDVAS 324
            R+ K   + AL I+ K   GIP EE+A+GL DV++
Sbjct:  445 RDSKSYCENALGIYEKPLLGIPPEEVASGLTDVSA 479
[Close]
BLAST of AM162056 vs. TrEMBL
Match: D7UCV7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_46.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00027175001 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.044e-11
Identity = 28/42 (66.67%), Postives = 34/42 (80.95%), Query Frame = 2 
Query:   65 DAYLSLARYDEAVFSYQKALTVFKSTKGENHTNSCLCFCKVS 190
            D YLSL R+DEAVFSYQKALTVFKSTKG+NH +    F +++
Sbjct:  302 DIYLSLCRFDEAVFSYQKALTVFKSTKGDNHPSVASVFIRLA 343

HSP 2 Score: 32.3426 bits (72), Expect = 1.044e-11
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 1 
Query:  220 RNLKLIAKIALRIFGKKNPGIPLEEIANGLIDVAS 324
            R  K   + ALRI+ K  PG   EEIANGL ++++
Sbjct:  353 RESKSYCENALRIYAKPVPGTTPEEIANGLTEISA 387
[Close]
BLAST of AM162056 vs. TrEMBL
Match: A5C301_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021292 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.063e-11
Identity = 28/42 (66.67%), Postives = 34/42 (80.95%), Query Frame = 2 
Query:   65 DAYLSLARYDEAVFSYQKALTVFKSTKGENHTNSCLCFCKVS 190
            D YLSL R+DEAVFSYQKALTVFKSTKG+NH +    F +++
Sbjct:   21 DIYLSLCRFDEAVFSYQKALTVFKSTKGDNHPSVASVFIRLA 62

HSP 2 Score: 32.3426 bits (72), Expect = 1.063e-11
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 1 
Query:  220 RNLKLIAKIALRIFGKKNPGIPLEEIANGLIDVAS 324
            R  K   + ALRI+ K  PG   EEIANGL ++++
Sbjct:   72 RESKSYCENALRIYAKPVPGTTPEEIANGLTEISA 106
[Close]
BLAST of AM162056 vs. TrEMBL
Match: D2DWB2_PHAVU (Kinesin light chain-like protein OS=Phaseolus vulgaris PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.250e-11
Identity = 46/91 (50.55%), Postives = 54/91 (59.34%), Query Frame = 3 
Query:    3 MVMAENRQELDVASVDCR-HRAMLICHWHDMMKRFFLIRKR*LFSNQPKERITPTVASVFVRLADLYNKIGKFKESKTYCENSTSNFREEK 272
            M MA N  E DVASVDC    A L    +D  +  F  +K        K    PTVASV+VRLADLYNKIGKFKE+K+YCEN+   F + K
Sbjct:  403 MAMAANGHEADVASVDCSIGDAYLALSRYD--EAVFSYQKALTVFKSTKGENHPTVASVYVRLADLYNKIGKFKEAKSYCENALRIFGKIK 491
[Close]
BLAST of AM162056 vs. TrEMBL
Match: Q9LII8_ARATH (Kinesin light chain-like protein OS=Arabidopsis thaliana GN=At3g27960 PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.989e-11
Identity = 24/42 (57.14%), Postives = 34/42 (80.95%), Query Frame = 2 
Query:   65 DAYLSLARYDEAVFSYQKALTVFKSTKGENHTNSCLCFCKVS 190
            DAY+SLAR+DEA+F+YQKAL VFK  KGE H++  L + +++
Sbjct:  377 DAYMSLARFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLA 418

HSP 2 Score: 34.2686 bits (77), Expect = 4.989e-11
Identity = 14/36 (38.89%), Postives = 25/36 (69.44%), Query Frame = 1 
Query:  217 SRNLKLIAKIALRIFGKKNPGIPLEEIANGLIDVAS 324
            +R+ K   + AL+I+ K  PG P+EE+A G I++++
Sbjct:  427 TRDSKSYCENALKIYLKPTPGTPMEEVATGFIEISA 462
[Close]
BLAST of AM162056 vs. TrEMBL
Match: Q7XA91_ARATH (At3g27960 OS=Arabidopsis thaliana GN=At3g27960 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.989e-11
Identity = 24/42 (57.14%), Postives = 34/42 (80.95%), Query Frame = 2 
Query:   65 DAYLSLARYDEAVFSYQKALTVFKSTKGENHTNSCLCFCKVS 190
            DAY+SLAR+DEA+F+YQKAL VFK  KGE H++  L + +++
Sbjct:  377 DAYMSLARFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLA 418

HSP 2 Score: 34.2686 bits (77), Expect = 4.989e-11
Identity = 14/36 (38.89%), Postives = 25/36 (69.44%), Query Frame = 1 
Query:  217 SRNLKLIAKIALRIFGKKNPGIPLEEIANGLIDVAS 324
            +R+ K   + AL+I+ K  PG P+EE+A G I++++
Sbjct:  427 TRDSKSYCENALKIYLKPTPGTPMEEVATGFIEISA 462
[Close]
BLAST of AM162056 vs. TrEMBL
Match: B9HID7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1084820 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.100e-11
Identity = 41/83 (49.40%), Postives = 52/83 (62.65%), Query Frame = 3 
Query:    3 MVMAENRQELDVASVDCR-HRAMLICHWHDMMKRFFLIRKR*LFSNQPKERITPTVASVFVRLADLYNKIGKFKESKTYCENS 248
            M MA N Q++DVAS+DC    A L    +D  +  F  +K        K    P VASVFVRLADLYNKIG+F++SK+YCEN+
Sbjct:  336 MAMAANGQDMDVASIDCSIGDAYLSLARYD--EAIFTYQKALTVFKSRKGENHPAVASVFVRLADLYNKIGRFRDSKSYCENA 416

HSP 2 Score: 64.3142 bits (155), Expect = 4.444e-9
Identity = 42/122 (34.43%), Postives = 61/122 (50.00%), Query Frame = 2 
Query:   65 DAYLSLARYDEAVFSYQKALTVFKSTKGENHTNSCLCFC-------KVSRFIQQNRKVQGI*NLLRK*HFEFSGRKIQEFL*KRLLMV**MLLAIYQIYE*FGKRIEVTQKSIETYHNNVPG 409
            DAYLSLARYDEA+F+YQKALTVFKS KGENH      F        K+ RF       +    +  K          +E +    + V     A+YQ    + + +++ +K+++ Y  + PG
Sbjct:  356 DAYLSLARYDEAIFTYQKALTVFKSRKGENHPAVASVFVRLADLYNKIGRFRDSKSYCENALRIYEKPRPAGGVWPSEEIIASGFIDV----SALYQSMNEYDRALKLLKKALKIYGTDAPG 473
[Close]
BLAST of AM162056 vs. TrEMBL
Match: D7TWQ0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_66.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032474001 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.058e-10
Identity = 39/84 (46.43%), Postives = 51/84 (60.71%), Query Frame = 3 
Query:    3 MVMAENRQELDVASVDCR--HRAMLICHWHDMMKRFFLIRKR*LFSNQPKERITPTVASVFVRLADLYNKIGKFKESKTYCENS 248
            M MA N QE+D AS+DC      + +  + + +   F  +K        K    PTVASVFVRLADLYNK+GK +ESK+YCEN+
Sbjct:  363 MAMAANGQEIDAASIDCSIGDTYLSLARYDEAV---FSYQKALTVFKSTKGENHPTVASVFVRLADLYNKVGKLRESKSYCENA 443
[Close]
BLAST of AM162056 vs. TrEMBL
Match: A5AM52_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_036421 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.058e-10
Identity = 39/84 (46.43%), Postives = 51/84 (60.71%), Query Frame = 3 
Query:    3 MVMAENRQELDVASVDCR--HRAMLICHWHDMMKRFFLIRKR*LFSNQPKERITPTVASVFVRLADLYNKIGKFKESKTYCENS 248
            M MA N QE+D AS+DC      + +  + + +   F  +K        K    PTVASVFVRLADLYNK+GK +ESK+YCEN+
Sbjct:   46 MAMAANGQEIDAASIDCSIGDTYLSLARYDEAV---FSYQKALTVFKSTKGENHPTVASVFVRLADLYNKVGKLRESKSYCENA 126
[Close]
BLAST of AM162056 vs. TrEMBL
Match: B9GL22_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_709112 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.436e-10
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 2 
Query:   71 YLSLARYDEAVFSYQKALTVFKSTKGENHTNSCLCFCKVSRFIQQNRKVQ 220
            Y+SL R+DEAVFSYQKALTVFKS+KG+NH +    F +++    +  K++
Sbjct:   45 YMSLCRFDEAVFSYQKALTVFKSSKGDNHPSVASVFVRLADLYHRTGKLR 94

HSP 2 Score: 30.8018 bits (68), Expect = 2.436e-10
Identity = 15/35 (42.86%), Postives = 21/35 (60.00%), Query Frame = 1 
Query:  220 RNLKLIAKIALRIFGKKNPGIPLEEIANGLIDVAS 324
            R  K   + ALRI+ K  PG   EEIA GL ++++
Sbjct:   94 RESKSYCENALRIYAKPVPGTTAEEIAGGLTEISA 128

HSP 3 Score: 56.9954 bits (136), Expect = 7.096e-7
Identity = 34/84 (40.48%), Postives = 50/84 (59.52%), Query Frame = 3 
Query:    3 MVMAENRQELDVASVDCR--HRAMLICHWHDMMKRFFLIRKR*LFSNQPKERITPTVASVFVRLADLYNKIGKFKESKTYCENS 248
            M M  N Q+ +VA++D    +  M +C + + +   F  +K        K    P+VASVFVRLADLY++ GK +ESK+YCEN+
Sbjct:   23 MAMIANGQDNEVAAIDVSIGNIYMSLCRFDEAV---FSYQKALTVFKSSKGDNHPSVASVFVRLADLYHRTGKLRESKSYCENA 103
[Close]
BLAST of AM162056 vs. TAIR peptide
Match: AT3G27960.1 (| Symbols: | Tetratricopeptide repeat (TPR)-like superfamily protein | chr3:10380513-10382593 REVERSE LENGTH=663)

HSP 1 Score: 56.6102 bits (135), Expect = 2.988e-13
Identity = 24/42 (57.14%), Postives = 34/42 (80.95%), Query Frame = 2 
Query:   65 DAYLSLARYDEAVFSYQKALTVFKSTKGENHTNSCLCFCKVS 190
            DAY+SLAR+DEA+F+YQKAL VFK  KGE H++  L + +++
Sbjct:  377 DAYMSLARFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLA 418

HSP 2 Score: 34.2686 bits (77), Expect = 2.988e-13
Identity = 14/36 (38.89%), Postives = 25/36 (69.44%), Query Frame = 1 
Query:  217 SRNLKLIAKIALRIFGKKNPGIPLEEIANGLIDVAS 324
            +R+ K   + AL+I+ K  PG P+EE+A G I++++
Sbjct:  427 TRDSKSYCENALKIYLKPTPGTPMEEVATGFIEISA 462

HSP 3 Score: 56.225 bits (134), Expect = 7.304e-9
Identity = 36/86 (41.86%), Postives = 47/86 (54.65%), Query Frame = 3 
Query:    3 MVMAENRQELDVASVDCRHRAMLICHWHDMMKRF----FLIRKR*LFSNQPKERITPTVASVFVRLADLYNKIGKFKESKTYCENS 248
            M M+      DVA+VDC      I   +  + RF    F  +K      Q K     +VA V+VRLADLYNKIGK ++SK+YCEN+
Sbjct:  357 MAMSSQNHREDVAAVDCS-----IGDAYMSLARFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENA 437
[Close]
BLAST of AM162056 vs. TAIR peptide
Match: AT4G10840.2 (| Symbols: | Tetratricopeptide repeat (TPR)-like superfamily protein | chr4:6656614-6658702 FORWARD LENGTH=531)

HSP 1 Score: 58.9214 bits (141), Expect = 1.127e-9
Identity = 35/84 (41.67%), Postives = 50/84 (59.52%), Query Frame = 3 
Query:    3 MVMAENRQELDVASVDCR--HRAMLICHWHDMMKRFFLIRKR*LFSNQPKERITPTVASVFVRLADLYNKIGKFKESKTYCENS 248
            M M  + QE +VAS+D    +  M +C + + +   F  +K        K    PTVASVFVRLA+LY++ GK +ESK+YCEN+
Sbjct:  295 MAMIASGQESEVASIDVSIGNIYMSLCRFDEAV---FSYQKALTVFKASKGETHPTVASVFVRLAELYHRTGKLRESKSYCENA 375
[Close]
BLAST of AM162056 vs. TAIR peptide
Match: AT4G10840.1 (| Symbols: | Tetratricopeptide repeat (TPR)-like superfamily protein | chr4:6656614-6659033 FORWARD LENGTH=609)

HSP 1 Score: 58.9214 bits (141), Expect = 1.127e-9
Identity = 35/84 (41.67%), Postives = 50/84 (59.52%), Query Frame = 3 
Query:    3 MVMAENRQELDVASVDCR--HRAMLICHWHDMMKRFFLIRKR*LFSNQPKERITPTVASVFVRLADLYNKIGKFKESKTYCENS 248
            M M  + QE +VAS+D    +  M +C + + +   F  +K        K    PTVASVFVRLA+LY++ GK +ESK+YCEN+
Sbjct:  295 MAMIASGQESEVASIDVSIGNIYMSLCRFDEAV---FSYQKALTVFKASKGETHPTVASVFVRLAELYHRTGKLRESKSYCENA 375
[Close]
BLAST of AM162056 vs. TAIR peptide
Match: AT1G27500.1 (| Symbols: | Tetratricopeptide repeat (TPR)-like superfamily protein | chr1:9551629-9553654 REVERSE LENGTH=650)

HSP 1 Score: 50.447 bits (119), Expect = 4.008e-7
Identity = 34/86 (39.53%), Postives = 45/86 (52.33%), Query Frame = 3 
Query:    3 MVMAENRQELDVASVDCRHRAMLICHWHDMMKRF----FLIRKR*LFSNQPKERITPTVASVFVRLADLYNKIGKFKESKTYCENS 248
            M MA N QE +VA VD       I   +  + RF       +K        K    P V SV++RLADLYN+ GK +E+K+YCEN+
Sbjct:  347 MAMAANGQESEVAFVDTS-----IGDSYLSLSRFDEAICAYQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENA 427
The following BLAST results are available for this feature:
BLAST of AM162056 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9I395_POPTR6.172e-1244.93Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7UCV7_VITVI1.044e-1166.67Whole genome shotgun sequence of line PN40024, sca... [more]
A5C301_VITVI1.063e-1166.67Putative uncharacterized protein OS=Vitis vinifera... [more]
D2DWB2_PHAVU1.250e-1150.55Kinesin light chain-like protein OS=Phaseolus vulg... [more]
Q9LII8_ARATH4.989e-1157.14Kinesin light chain-like protein OS=Arabidopsis th... [more]
Q7XA91_ARATH4.989e-1157.14At3g27960 OS=Arabidopsis thaliana GN=At3g27960 PE=... [more]
B9HID7_POPTR8.100e-1149.40Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7TWQ0_VITVI1.058e-1046.43Whole genome shotgun sequence of line PN40024, sca... [more]
A5AM52_VITVI1.058e-1046.43Putative uncharacterized protein OS=Vitis vinifera... [more]
B9GL22_POPTR2.436e-1052.00Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
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BLAST of AM162056 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4
Match NameE-valueIdentityDescription
AT3G27960.12.988e-1357.14| Symbols: | Tetratricopeptide repeat (TPR)-like ... [more]
AT4G10840.21.127e-941.67| Symbols: | Tetratricopeptide repeat (TPR)-like ... [more]
AT4G10840.11.127e-941.67| Symbols: | Tetratricopeptide repeat (TPR)-like ... [more]
AT1G27500.14.008e-739.53| Symbols: | Tetratricopeptide repeat (TPR)-like ... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013026Tetratricopeptide repeat-containingPROFILEPS50293TPR_REGIONcoord: 1..27
score: 9

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM162056 ID=AM162056; Name=AM162056; organism=Pisum sativum; type=EST; length=419bp
GTATGGTCATGGCGGAAAATCGCCAGGAACTAGATGTTGCGTCTGTTGAT
TGCAGACATAGAGCGATGCTTATTTGTCATTGGCACGATATGATGAAGCG
GTTTTTTCTTATCAGAAAGCGCTAACTGTTTTCAAATCAACCAAAGGAGA
GAATCACACCAACAGTTGCCTCTGTTTTTGTAAGGTTAGCAGATTTATAC
AACAAAATAGGAAAGTTCAAGGAATCTAAAACTTATTGCGAAAATAGCAC
TTCGAATTTTCGGGAAGAAAAATCCAGGAATTCCTCTAGAAGAGATTGCT
AATGGTTTGATAGATGTTGCTAGCAATTTACCAAATCTATGAATGATTTG
GAAAAAGGATTGAAGTTACTCAAAAAAGCATTGAAACATATCATAACAAT
GTTCCTGGTNAAACAAAGT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: INTERPRO
TermDefinition
IPR013026TPR-contain_dom