GH720445

EST Overview


Name	GH720445
Unique Name	GH720445
Type	EST
Organism	Pisum sativum (pea)
Sequence length	464

Libraries


Library Name	Type
LIBEST_024270 552I	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720445 vs. TrEMBL
Match: Q8GSM9_MEDTR (Squalene monooxygenase 2 OS=Medicago truncatula GN=sqp2 PE=2 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 1.652e-51
Identity = 100/104 (96.15%), Postives = 103/104 (99.04%), Query Frame = -3

Query:  136 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPPVS 447
            ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAV+GVGRLLLPFPSPKRVW+GARLLSGASGIILPIIKAEGIRQMFFPATVPAYYR PPV+
Sbjct:  421 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVFGVGRLLLPFPSPKRVWIGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRAPPVN 524

[Close]

BLAST of GH720445 vs. TrEMBL
Match: Q2HY08_MEDSA (Squalene epoxidase OS=Medicago sativa GN=SE1 PE=4 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 1.652e-51
Identity = 100/104 (96.15%), Postives = 103/104 (99.04%), Query Frame = -3

Query:  136 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPPVS 447
            ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAV+GVGRLLLPFPSPKRVW+GARLLSGASGIILPIIKAEGIRQMFFPATVPAYYR PPV+
Sbjct:  419 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVFGVGRLLLPFPSPKRVWIGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRAPPVN 522

[Close]

BLAST of GH720445 vs. TrEMBL
Match: B9R6V0_RICCO (Squalene monooxygenase, putative OS=Ricinus communis GN=RCOM_1585750 PE=4 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 7.673e-49
Identity = 93/103 (90.29%), Postives = 99/103 (96.12%), Query Frame = -3

Query:  139 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPPV 447
            AR EMRQACFDYLSLGG+FS GPISLLSGLNPRPLSLV+HFFAVA+YGVGRLLLPFPSPKRVW+GARL+SGASGII PIIKAEG+RQMFFPATVPAYYR PPV
Sbjct:  429 ARNEMRQACFDYLSLGGVFSTGPISLLSGLNPRPLSLVVHFFAVAIYGVGRLLLPFPSPKRVWVGARLISGASGIIFPIIKAEGVRQMFFPATVPAYYRAPPV 531

[Close]

BLAST of GH720445 vs. TrEMBL
Match: D7T3J5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_88.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036971001 PE=4 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 1.709e-48
Identity = 92/102 (90.20%), Postives = 99/102 (97.06%), Query Frame = -3

Query:  142 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPP 447
            ARKEMR+ACFDYLSLGG+FS GPI+LLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKR+W+GARL+SGASGII PIIKAEG+RQMFFPATVPAYYR PP
Sbjct:  386 ARKEMREACFDYLSLGGIFSNGPIALLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRMWIGARLISGASGIIFPIIKAEGVRQMFFPATVPAYYRGPP 487

[Close]

BLAST of GH720445 vs. TrEMBL
Match: Q8GSM8_MEDTR (Squalene monooxygenase 1 OS=Medicago truncatula GN=sqp1 PE=2 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 4.974e-48
Identity = 92/102 (90.20%), Postives = 98/102 (96.08%), Query Frame = -3

Query:  142 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPP 447
            ARKEMRQACFDYLSLGGLFSEGP+SLLSGLNP PLSLVLHFFAVA+YGVGRLLLPFPSPKR+W+G RL++ ASGIILPIIKAEGIRQMFFPATVPAYYR PP
Sbjct:  416 ARKEMRQACFDYLSLGGLFSEGPVSLLSGLNPCPLSLVLHFFAVAIYGVGRLLLPFPSPKRLWIGIRLIASASGIILPIIKAEGIRQMFFPATVPAYYRAPP 517

[Close]

BLAST of GH720445 vs. TrEMBL
Match: B9H6N5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831444 PE=4 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 4.974e-48
Identity = 90/103 (87.38%), Postives = 98/103 (95.15%), Query Frame = -3

Query:  139 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPPV 447
            A KEMRQACFDYLSLGG+FS GP+SLLSGLNPRPLSLV HFFAVA+YGVGRLLLPFPSPKR+W+GARL+SGASGII PII+AEG+RQMFFPATVPAYYR PPV
Sbjct:  423 AMKEMRQACFDYLSLGGVFSAGPVSLLSGLNPRPLSLVAHFFAVAIYGVGRLLLPFPSPKRIWIGARLISGASGIIFPIIRAEGVRQMFFPATVPAYYRAPPV 525

[Close]

BLAST of GH720445 vs. TrEMBL
Match: Q506K3_DATIN (Squalene monooxygenase OS=Datura inoxia PE=2 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 6.496e-48
Identity = 89/102 (87.25%), Postives = 98/102 (96.08%), Query Frame = -3

Query:  142 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPP 447
            ARKEMR+ACFDYLSLGG+FSEGP+SLLSGLNPRPLSLV HFFAVA+YGVGRLLLPFPSPKR+W+GARL+SGAS II PIIKAEG+RQMFFPAT+PAYYR PP
Sbjct:  424 ARKEMREACFDYLSLGGVFSEGPVSLLSGLNPRPLSLVCHFFAVAIYGVGRLLLPFPSPKRLWIGARLISGASAIIFPIIKAEGVRQMFFPATIPAYYRAPP 525

[Close]

BLAST of GH720445 vs. TrEMBL
Match: C4P9M2_GYNPE (Squalene epoxidase OS=Gynostemma pentaphyllum PE=2 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 8.484e-48
Identity = 88/103 (85.44%), Postives = 100/103 (97.09%), Query Frame = -3

Query:  139 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPPV 447
            AR+EMRQACFDYLSLGG+FS GP+SLLSGLNPRPLSLVLHFFAVA+YGVGRLL+PFPSP+RVW+GARL+SGASGII PIIKAEG+RQ+FFPAT+PAYYR PP+
Sbjct:  419 ARREMRQACFDYLSLGGVFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLIPFPSPRRVWIGARLISGASGIIFPIIKAEGVRQIFFPATLPAYYRAPPL 521

[Close]

BLAST of GH720445 vs. TrEMBL
Match: B9GNL3_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_411973 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.447e-47
Identity = 89/103 (86.41%), Postives = 99/103 (96.12%), Query Frame = -3

Query:  139 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPPV 447
            ARKEMRQACFDYLSLGG+ SEGP+SLLSGLNPRPLSLV HFFAVA+YGVGRLLLPFPSPKR+W+GARL+S ASGII PII++EG+RQMFFPATVPAYYR+PPV
Sbjct:  361 ARKEMRQACFDYLSLGGVCSEGPVSLLSGLNPRPLSLVAHFFAVAIYGVGRLLLPFPSPKRIWIGARLISSASGIIFPIIRSEGVRQMFFPATVPAYYRSPPV 463

[Close]

BLAST of GH720445 vs. TrEMBL
Match: D3YM76_9SOLA (Squalene epoxidase OS=Withania somnifera PE=2 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 1.890e-47
Identity = 89/102 (87.25%), Postives = 97/102 (95.10%), Query Frame = -3

Query:  142 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPP 447
            ARKEMR+ACFDYLSLGG+FS GP+SLLSGLNPRPLSLV HFFAVA+YGVGRLLLPFPSPKR+W+GARL+SGAS II PIIKAEG+RQMFFPATVPAYYR PP
Sbjct:  426 ARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLVCHFFAVAIYGVGRLLLPFPSPKRLWIGARLISGASAIIFPIIKAEGVRQMFFPATVPAYYRAPP 527

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG1_PANGI (Squalene monooxygenase OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 4.295e-46
Identity = 84/103 (81.55%), Postives = 96/103 (93.20%), Query Frame = -3

Query:  139 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPPV 447
            AR+EMR ACFDYLSLGG+ S+GPI+LLSGLNPRP+SL LHFFAVA+YGVGRLL+PFPSPKR+WLGARL+ GASGII PIIK+EG+RQMFFPA VPAYYR PP+
Sbjct:  436 ARQEMRNACFDYLSLGGICSQGPIALLSGLNPRPISLFLHFFAVAIYGVGRLLIPFPSPKRMWLGARLILGASGIIFPIIKSEGLRQMFFPAIVPAYYRAPPI 538

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG11_ARATH (Squalene monooxygenase 1,1 OS=Arabidopsis thaliana GN=SQP1,1 PE=2 SV=2)

HSP 1 Score: 106.301 bits (264), Expect = 6.673e-23
Identity = 47/100 (47.00%), Postives = 67/100 (67.00%), Query Frame = -3

Query:  148 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRT 447
            A++ MRQ C+DYLS GG  + G ++LL G+NPRP+SL+ H  A+ +  +G LL PFPSP R+W   RL   A  +++P +KAEG+ QM FP    AY ++
Sbjct:  410 AKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIGHLLSPFPSPLRIWHSLRLFGLAMKMLVPHLKAEGVSQMLFPVNAAAYSKS 509

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG12_BRANA (Squalene monooxygenase 1,2 OS=Brassica napus GN=SQP1,2 PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 3.312e-22
Identity = 48/100 (48.00%), Postives = 68/100 (68.00%), Query Frame = -3

Query:  148 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRT 447
            A++ MRQ CFDYLS GG  + G ++L+ G+NPRPLSL  H F +++  +G+LL PFP+P RVW   RLL  +  +++P +KAEGI QM  P    AY ++
Sbjct:  412 AKEAMRQGCFDYLSSGGFRTSGLMALIGGMNPRPLSLFYHLFVISLSSIGQLLSPFPTPLRVWHSLRLLDLSLKMLVPHLKAEGIGQMLSPTNAAAYRKS 511

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG12_ARATH (Squalene monooxygenase 1,2 OS=Arabidopsis thaliana GN=SQP1,2 PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 3.312e-22
Identity = 47/100 (47.00%), Postives = 68/100 (68.00%), Query Frame = -3

Query:  148 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRT 447
            A++ MRQ  +DYL  GG  + G ++LL G+NPRPLSLV H  A+ +  +G+LL PFPSP R+W   +L   A  +++P +KAEG+ QM FPA   AY+++
Sbjct:  411 AKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLSSIGQLLSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG13_ARATH (Squalene monooxygenase 2 OS=Arabidopsis thaliana GN=SQP2 PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 6.463e-18
Identity = 42/99 (42.42%), Postives = 62/99 (62.63%), Query Frame = -3

Query:  151 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYR 447
            AR+ MRQ CF+YL+ G   + G +++L G+NP PL+LVLH  A+ +  +G LL PFPSP+R W   R+L+ A  ++   +  EG ++M  P    AY R
Sbjct:  410 AREGMRQGCFNYLARGDFKTRGLMTILGGMNPHPLTLVLHLVAITLTSMGHLLSPFPSPRRFWHSLRILAWALQMLGAHLVDEGFKEMLIPTNAAAYRR 508

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG11_BRANA (Squalene monooxygenase 1,1 OS=Brassica napus GN=SQP1,1 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 8.441e-18
Identity = 44/100 (44.00%), Postives = 59/100 (59.00%), Query Frame = -3

Query:  148 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRT 447
            A++ MRQ CFDYLS GG  + G ++LL G+NPRPLSL+ H   + +  +G+LL PFPSP  +W   RL             AEG+ QM  PA   AY ++
Sbjct:  411 AKEAMRQGCFDYLSSGGFRTSGMMALLGGMNPRPLSLIFHLCGITLSSIGQLLSPFPSPLGIWHSLRLFG-----------AEGVSQMLSPAYAAAYRKS 499

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG1_RAT (Squalene monooxygenase OS=Rattus norvegicus GN=Sqle PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 2.462e-9
Identity = 34/84 (40.48%), Postives = 48/84 (57.14%), Query Frame = -3

Query:  199 KEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFP---SPKRVWLGARLLSGASGIILPIIKAE 441
            +++R+ACF Y  LGG    GP+ LLS L+P PL L+ HFF+VAVY         P    P+ ++    +L  A  II P+I +E
Sbjct:  484 RQLRKACFLYFKLGGECLTGPVGLLSILSPDPLLLIRHFFSVAVYATYFCFKSEPWATKPRALFSSGAILYKACSIIFPLIYSE 567

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG1_HUMAN (Squalene monooxygenase OS=Homo sapiens GN=SQLE PE=2 SV=3)

HSP 1 Score: 61.2326 bits (147), Expect = 2.462e-9
Identity = 34/83 (40.96%), Postives = 47/83 (56.63%), Query Frame = -3

Query:  199 EMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFP---SPKRVWLGARLLSGASGIILPIIKAE 438
            ++R+ACF Y  LGG    GP+ LLS L+P PL L+ HFFAVA+Y V       P    P+ +     +L  A  +I P+I +E
Sbjct:  486 QLRKACFLYFKLGGECVAGPVGLLSVLSPNPLVLIGHFFAVAIYAVYFCFKSEPWITKPRALLSSGAVLYKACSVIFPLIYSE 568

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG1_MOUSE (Squalene monooxygenase OS=Mus musculus GN=Sqle PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 7.162e-9
Identity = 32/83 (38.55%), Postives = 47/83 (56.63%), Query Frame = -3

Query:  199 EMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFP---SPKRVWLGARLLSGASGIILPIIKAE 438
            ++R+ACF Y  LGG    GP+ LLS L+P PL L+ HFF+VA+Y         P    P+ ++    +L  A  I+ P+I +E
Sbjct:  484 QLRKACFLYFKLGGECVTGPVGLLSILSPHPLVLIRHFFSVAIYATYFCFKSEPWATKPRALFSSGAVLYKACSILFPLIYSE 566

[Close]

BLAST of GH720445 vs. SwissProt
Match: ERG1_SCHPO (Probable squalene monooxygenase OS=Schizosaccharomyces pombe GN=SPBC713.12 PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.722e-8
Identity = 32/84 (38.10%), Postives = 46/84 (54.76%), Query Frame = -3

Query:  199 KEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPS---PKRVWLGARLLSGASGIILPIIKAE 441
            K +   C DY   GG   EGPI LL GL+  P  L+ HF+AV +YG+ + +L  P+   P R+     +   AS +I+P I +E
Sbjct:  371 KALESGCIDYFKRGGNCVEGPIRLLGGLDHSPSHLIGHFYAVCLYGIYQYVLSGPALLMPVRIIESLLIFLQASLVIIPYILSE 454

[Close]

BLAST of GH720445 vs. TAIR peptide
Match: AT1G58440.1 (| Symbols: XF1, SQE1 | FAD/NAD(P)-binding oxidoreductase family protein | chr1:21714094-21717246 REVERSE LENGTH=531)

HSP 1 Score: 176.022 bits (445), Expect = 7.953e-45
Identity = 83/106 (78.30%), Postives = 93/106 (87.74%), Query Frame = -3

Query:  130 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPPVSQE 447
            AR EMR+ACFDYL LGG+ + GP+SLLSGLNPRPL+LV HFFAVAVYGV RLL+PFPSPKR+WLGA+L+SGASGII PIIKAEG+RQMFFPATVPAYY   P   E
Sbjct:  422 ARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPSPKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAPTVGE 527

[Close]

BLAST of GH720445 vs. TAIR peptide
Match: AT4G37760.1 (| Symbols: SQE3 | squalene epoxidase 3 | chr4:17744085-17746426 FORWARD LENGTH=525)

HSP 1 Score: 173.326 bits (438), Expect = 5.155e-44
Identity = 80/102 (78.43%), Postives = 92/102 (90.20%), Query Frame = -3

Query:  142 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPP 447
            AR EMR+ACFDYLSLGG+ S GP++LLSGLNPRP+SLVLHFFAVA++GVGRLL+P PS KR+WLGARL+S ASGII PIIKAEG+RQMFFP T+PA YR PP
Sbjct:  416 ARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLPSVKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

[Close]

BLAST of GH720445 vs. TAIR peptide
Match: AT2G22830.1 (| Symbols: SQE2 | squalene epoxidase 2 | chr2:9723947-9726270 REVERSE LENGTH=585)

HSP 1 Score: 167.548 bits (423), Expect = 2.828e-42
Identity = 78/102 (76.47%), Postives = 89/102 (87.25%), Query Frame = -3

Query:  142 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRTPP 447
            AR EMR+ACFDYLSLGG+FS GP++LLSGLNPRPLSLVLHFFAVA+Y V RL+LPFPS +  WLGAR++S AS II PIIKAEG+RQMFFP T+PA YR PP
Sbjct:  484 ARTEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFWLGARIISSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

[Close]

BLAST of GH720445 vs. TAIR peptide
Match: AT5G24150.1 (| Symbols: SQP1, SQE5 | FAD/NAD(P)-binding oxidoreductase family protein | chr5:8172594-8175395 REVERSE LENGTH=516)

HSP 1 Score: 106.301 bits (264), Expect = 7.739e-24
Identity = 47/100 (47.00%), Postives = 67/100 (67.00%), Query Frame = -3

Query:  148 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRT 447
            A++ MRQ C+DYLS GG  + G ++LL G+NPRP+SL+ H  A+ +  +G LL PFPSP R+W   RL   A  +++P +KAEG+ QM FP    AY ++
Sbjct:  410 AKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIGHLLSPFPSPLRIWHSLRLFGLAMKMLVPHLKAEGVSQMLFPVNAAAYSKS 509

[Close]

BLAST of GH720445 vs. TAIR peptide
Match: AT5G24160.1 (| Symbols: SQE6 | squalene monoxygenase 6 | chr5:8183291-8186601 REVERSE LENGTH=517)

HSP 1 Score: 103.99 bits (258), Expect = 3.841e-23
Identity = 47/100 (47.00%), Postives = 68/100 (68.00%), Query Frame = -3

Query:  148 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYRT 447
            A++ MRQ  +DYL  GG  + G ++LL G+NPRPLSLV H  A+ +  +G+LL PFPSP R+W   +L   A  +++P +KAEG+ QM FPA   AY+++
Sbjct:  411 AKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLSSIGQLLSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510

[Close]

BLAST of GH720445 vs. TAIR peptide
Match: AT5G24140.1 (| Symbols: SQP2 | squalene monooxygenase 2 | chr5:8164547-8167669 REVERSE LENGTH=516)

HSP 1 Score: 89.7373 bits (221), Expect = 7.495e-19
Identity = 42/99 (42.42%), Postives = 62/99 (62.63%), Query Frame = -3

Query:  151 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILPIIKAEGIRQMFFPATVPAYYR 447
            AR+ MRQ CF+YL+ G   + G +++L G+NP PL+LVLH  A+ +  +G LL PFPSP+R W   R+L+ A  ++   +  EG ++M  P    AY R
Sbjct:  410 AREGMRQGCFNYLARGDFKTRGLMTILGGMNPHPLTLVLHLVAITLTSMGHLLSPFPSPRRFWHSLRILAWALQMLGAHLVDEGFKEMLIPTNAAAYRR 508

[Close]

BLAST of GH720445 vs. TAIR peptide
Match: AT5G24150.2 (| Symbols: SQP1 | FAD/NAD(P)-binding oxidoreductase family protein | chr5:8172670-8175395 REVERSE LENGTH=490)

HSP 1 Score: 84.3445 bits (207), Expect = 3.149e-17
Identity = 37/78 (47.44%), Postives = 51/78 (65.38%), Query Frame = -3

Query:  214 ARKEMRQACFDYLSLGGLFSEGPISLLSGLNPRPLSLVLHFFAVAVYGVGRLLLPFPSPKRVWLGARLLSGASGIILP 447
            A++ MRQ C+DYLS GG  + G ++LL G+NPRP+SL+ H  A+ +  +G LL PFPSP R+W   RL  G   +  P
Sbjct:  410 AKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIGHLLSPFPSPLRIWHSLRLFVGYENVGSP 487

The following BLAST results are available for this feature:

BLAST of GH720445 vs. TrEMBL
BLAST of GH720445 vs. SwissProt
BLAST of GH720445 vs. TAIR peptide

BLAST of GH720445 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q8GSM9_MEDTR	1.652e-51	96.15	Squalene monooxygenase 2 OS=Medicago truncatula GN...	[more]
Q2HY08_MEDSA	1.652e-51	96.15	Squalene epoxidase OS=Medicago sativa GN=SE1 PE=4 ...	[more]
B9R6V0_RICCO	7.673e-49	90.29	Squalene monooxygenase, putative OS=Ricinus commun...	[more]
D7T3J5_VITVI	1.709e-48	90.20	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q8GSM8_MEDTR	4.974e-48	90.20	Squalene monooxygenase 1 OS=Medicago truncatula GN...	[more]
B9H6N5_POPTR	4.974e-48	87.38	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q506K3_DATIN	6.496e-48	87.25	Squalene monooxygenase OS=Datura inoxia PE=2 SV=1	[more]
C4P9M2_GYNPE	8.484e-48	85.44	Squalene epoxidase OS=Gynostemma pentaphyllum PE=2...	[more]
B9GNL3_POPTR	1.447e-47	86.41	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D3YM76_9SOLA	1.890e-47	87.25	Squalene epoxidase OS=Withania somnifera PE=2 SV=1	[more]

BLAST of GH720445 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ERG1_PANGI	4.295e-46	81.55	Squalene monooxygenase OS=Panax ginseng PE=2 SV=1	[more]
ERG11_ARATH	6.673e-23	47.00	Squalene monooxygenase 1,1 OS=Arabidopsis thaliana...	[more]
ERG12_BRANA	3.312e-22	48.00	Squalene monooxygenase 1,2 OS=Brassica napus GN=SQ...	[more]
ERG12_ARATH	3.312e-22	47.00	Squalene monooxygenase 1,2 OS=Arabidopsis thaliana...	[more]
ERG13_ARATH	6.463e-18	42.42	Squalene monooxygenase 2 OS=Arabidopsis thaliana G...	[more]
ERG11_BRANA	8.441e-18	44.00	Squalene monooxygenase 1,1 OS=Brassica napus GN=SQ...	[more]
ERG1_RAT	2.462e-9	40.48	Squalene monooxygenase OS=Rattus norvegicus GN=Sql...	[more]
ERG1_HUMAN	2.462e-9	40.96	Squalene monooxygenase OS=Homo sapiens GN=SQLE PE=...	[more]
ERG1_MOUSE	7.162e-9	38.55	Squalene monooxygenase OS=Mus musculus GN=Sqle PE=...	[more]
ERG1_SCHPO	2.722e-8	38.10	Probable squalene monooxygenase OS=Schizosaccharom...	[more]

BLAST of GH720445 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT1G58440.1	7.953e-45	78.30	\| Symbols: XF1, SQE1 \| FAD/NAD(P)-binding oxidored...	[more]
AT4G37760.1	5.155e-44	78.43	\| Symbols: SQE3 \| squalene epoxidase 3 \| chr4:1774...	[more]
AT2G22830.1	2.828e-42	76.47	\| Symbols: SQE2 \| squalene epoxidase 2 \| chr2:9723...	[more]
AT5G24150.1	7.739e-24	47.00	\| Symbols: SQP1, SQE5 \| FAD/NAD(P)-binding oxidore...	[more]
AT5G24160.1	3.841e-23	47.00	\| Symbols: SQE6 \| squalene monoxygenase 6 \| chr5:8...	[more]
AT5G24140.1	7.495e-19	42.42	\| Symbols: SQP2 \| squalene monooxygenase 2 \| chr5:...	[more]
AT5G24150.2	3.149e-17	47.44	\| Symbols: SQP1 \| FAD/NAD(P)-binding oxidoreductas...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013698	Squalene epoxidase	PFAM	PF08491	SE	coord: 5..68 score: 1.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720445 ID=GH720445; Name=GH720445; organism=Pisum sativum; type=EST; length=464bp
TAGCGTGGTCGCGGCCGAGGTACCAGTTACAACCTACAAAGAATACACAC
TAGGCCAAAATGTATACAATCTATCAGCATCAGCCTTCGTTCAATTTCTC
TCTAAAGTTCGGATGATAAAGCTAACTCACTCTTGTGAAACCGGAGGCGT
TCTGTAATAAGCTGGAACAGTGGCAGGGAAAAACATTTGGCGAATTCCTT
CAGCCTTAATTATGGGCAAGATGATTCCAGATGCACCAGAGAGTAATCGA
GCTCCAAGCCACACCCGTTTAGGTGAAGGAAATGGTAATAGTAAACGACC
AACACCATATACTGCGACAGCAAAGAAATGGAGAACCAAACTTAAGGGAC
GAGGGTTTAATCCTGAAAGTAAAGAGATCGGTCCTTCTGAGAATAGGCCC
CCAAGGCTCAGATAATCAAAGCAAGCTTGGCGCATTTCCTTCCTTGCACC
TGCCCGGGCGGCCG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004506	squalene monooxygenase activity
GO:0050660	flavin adenine dinucleotide binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR013698	Squalene_epoxidase

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