GH720661

EST Overview


Name	GH720661
Unique Name	GH720661
Type	EST
Organism	Pisum sativum (pea)
Sequence length	400

Libraries


Library Name	Type
LIBEST_024272 BACI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720661 vs. TrEMBL
Match: B9SWB7_RICCO (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, putative OS=Ricinus communis GN=RCOM_0154370 PE=3 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 4.225e-47
Identity = 90/126 (71.43%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   VRGDEPYEELEILK +DATM+LHDLP TED++ RVHAARRLFE+LS+NSL+FPVIHHI FPNGIHRDDLVIGAG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  519 LVVSVRGDEPYEELEILKQIDATMILHDLPNTEDKIGRVHAARRLFEFLSDNSLNFPVIHHINFPNGIHRDDLVIGAGANAGALLVDGLGDGILLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 644

[Close]

BLAST of GH720661 vs. TrEMBL
Match: A9ZN14_HEVBR (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Hevea brasiliensis GN=HbHDS PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 7.206e-47
Identity = 88/126 (69.84%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +    RGDEPYEELEILK +DATM+LHDLP+TED++ RVHAARRLFE+LS+N+L+FPVIHHIQFPN IHRDDLVIGAG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSARGDEPYEELEILKDIDATMILHDLPFTEDKIGRVHAARRLFEFLSDNALNFPVIHHIQFPNAIHRDDLVIGAGTNAGALLVDGLGDGILLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: D7SKY1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025182001 PE=4 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 3.576e-46
Identity = 88/126 (69.84%), Postives = 104/126 (82.54%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   VRGDEPYE+LEILK VDATM+LHDLP TE+++SRVH+ARRLFEYLS+N+L+FPVIHHIQFP GIHRDDLVIGAG++               EA ++DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSVRGDEPYEDLEILKEVDATMILHDLPLTEEKISRVHSARRLFEYLSDNALNFPVIHHIQFPKGIHRDDLVIGAGTNAGALLVDGLGDGLLLEAPEQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: A5BBY6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005654 PE=3 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 3.576e-46
Identity = 88/126 (69.84%), Postives = 104/126 (82.54%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   VRGDEPYE+LEILK VDATM+LHDLP TE+++SRVH+ARRLFEYLS+N+L+FPVIHHIQFP GIHRDDLVIGAG++               EA ++DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSVRGDEPYEDLEILKEVDATMILHDLPLTEEKISRVHSARRLFEYLSDNALNFPVIHHIQFPKGIHRDDLVIGAGTNAGALLVDGLGDGLLLEAPEQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q9FF59_ARATH (GcpE protein OS=Arabidopsis thaliana PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  492 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 617

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q8RXG8_ARATH (At5g60600 OS=Arabidopsis thaliana GN=At5g60600 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q8LPQ4_ARATH (AT5g60600/mup24_10 OS=Arabidopsis thaliana GN=At5g60600 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q8GZR7_ARATH (GcpE OS=Arabidopsis thaliana GN=At5g60600 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  516 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 641

[Close]

BLAST of GH720661 vs. TrEMBL
Match: B3H725_ARATH (Uncharacterized protein At5g60600.3 OS=Arabidopsis thaliana GN=At5g60600 PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 7.967e-46
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TrEMBL
Match: Q84XR5_CATRO (GCPE protein OS=Catharanthus roseus PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 3.954e-45
Identity = 87/126 (69.05%), Postives = 101/126 (80.16%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   VRGDEPYEEL+ILK VD TM+LH+LP+TE+++ RVHAARRLFEYLSENSL+FPVIHH+QF N IHRDDLVIGAGS                EA D+DF+F+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSVRGDEPYEELDILKNVDVTMILHNLPHTEEKIGRVHAARRLFEYLSENSLNFPVIHHMQFANAIHRDDLVIGAGSDAGALLVDGLGDGVMLEAHDQDFEFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TAIR peptide
Match: AT5G60600.3 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363019 FORWARD LENGTH=717)

HSP 1 Score: 186.422 bits (472), Expect = 4.215e-48
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

[Close]

BLAST of GH720661 vs. TAIR peptide
Match: AT5G60600.2 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363274 FORWARD LENGTH=740)

HSP 1 Score: 186.422 bits (472), Expect = 4.215e-48
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  516 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 641

[Close]

BLAST of GH720661 vs. TAIR peptide
Match: AT5G60600.1 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363274 FORWARD LENGTH=741)

HSP 1 Score: 186.422 bits (472), Expect = 4.215e-48
Identity = 87/126 (69.05%), Postives = 103/126 (81.75%), Query Frame = 2

Query:    8 VAAEVRGDEPYEELEILKGVDATMVLHDLPYTEDRVSRVHAARRLFEYLSENSLDFPVIHHIQFPNGIHRDDLVIGAGSHXXXXXXXXXXXXXXXEASDKDFDFIRNTSFNLLQGCRMRNTKTEYL 385
            +   +RGDEPYEELEILK +DATM+LHD+P+TED+VSRVHAARRLFE+LSENS++FPVIHHI FP GIHRD+LVI AG++               EA D+DFDF+RNTSFNLLQGCRMRNTKTEY+
Sbjct:  517 LVVSLRGDEPYEELEILKNIDATMILHDVPFTEDKVSRVHAARRLFEFLSENSVNFPVIHHINFPTGIHRDELVIHAGTYAGGLLVDGLGDGVMLEAPDQDFDFLRNTSFNLLQGCRMRNTKTEYV 642

The following BLAST results are available for this feature:

BLAST of GH720661 vs. TrEMBL
BLAST of GH720661 vs. TAIR peptide

BLAST of GH720661 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SWB7_RICCO	4.225e-47	71.43	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
A9ZN14_HEVBR	7.206e-47	69.84	4-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha...	[more]
D7SKY1_VITVI	3.576e-46	69.84	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BBY6_VITVI	3.576e-46	69.84	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q9FF59_ARATH	7.967e-46	69.05	GcpE protein OS=Arabidopsis thaliana PE=3 SV=1	[more]
Q8RXG8_ARATH	7.967e-46	69.05	At5g60600 OS=Arabidopsis thaliana GN=At5g60600 PE=...	[more]
Q8LPQ4_ARATH	7.967e-46	69.05	AT5g60600/mup24_10 OS=Arabidopsis thaliana GN=At5g...	[more]
Q8GZR7_ARATH	7.967e-46	69.05	GcpE OS=Arabidopsis thaliana GN=At5g60600 PE=2 SV=...	[more]
B3H725_ARATH	7.967e-46	69.05	Uncharacterized protein At5g60600.3 OS=Arabidopsis...	[more]
Q84XR5_CATRO	3.954e-45	69.05	GCPE protein OS=Catharanthus roseus PE=2 SV=1	[more]

BLAST of GH720661 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G60600.3	4.215e-48	69.05	\| Symbols: GCPE, ISPG, CSB3, CLB4, HDS \| 4-hydroxy...	[more]
AT5G60600.2	4.215e-48	69.05	\| Symbols: GCPE, ISPG, CSB3, CLB4, HDS \| 4-hydroxy...	[more]
AT5G60600.1	4.215e-48	69.05	\| Symbols: GCPE, ISPG, CSB3, CLB4, HDS \| 4-hydroxy...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 83..97 score
None	No IPR available	SEG	seg	seg	coord: 33..51 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720661 ID=GH720661; Name=GH720661; organism=Pisum sativum; type=EST; length=400bp
TAGCGTGGTCGCGGCCGAGGTACGTGGCGACGAGCCTTATGAAGAACTGG
AAATTCTCAAAGGTGTTGATGCAACTATGGTTCTCCATGACTTGCCTTAT
ACTGAAGACAGAGTTAGTAGAGTGCACGCAGCAAGGCGGTTATTCGAGTA
TCTATCAGAAAACTCGCTAGACTTTCCTGTCATTCACCACATTCAGTTTC
CAAATGGAATTCACAGGGATGACCTAGTGATTGGTGCTGGCTCTCATGCT
GGAGCCCTGCTGGTTGACGGGCTTGGAGACGGTCTTCTTTTAGAAGCTTC
AGACAAAGATTTTGACTTCATTAGAAATACATCTTTCAATTTGCTGCAAG
GATGCAGAATGAGAAATACAAAAACAGAGTACCTGCCCGGGCGGCCGCTC

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