AM161949

EST Overview
NameAM161949
Unique NameAM161949
TypeEST
OrganismPisum sativum (pea)
Sequence length345
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
Homology
[Close]
BLAST of AM161949 vs. TrEMBL
Match: A9ZN14_HEVBR (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Hevea brasiliensis GN=HbHDS PE=2 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 2.973e-45
Identity = 96/109 (88.07%), Postives = 102/109 (93.58%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMSV LDQLK PELLYKSLAAKL+VGMPFKDLATVDSILL+ELPPV+D DARLALKRL+DISMGVIVPLSEQLTKPLPNA+VLVNLKELS GA KLLP+GTRL VS
Sbjct:  412 GSVLMSVCLDQLKAPELLYKSLAAKLVVGMPFKDLATVDSILLRELPPVEDNDARLALKRLIDISMGVIVPLSEQLTKPLPNAMVLVNLKELSTGAHKLLPEGTRLVVS 520
[Close]
BLAST of AM161949 vs. TrEMBL
Match: Q8GZR6_SOLLC (GcpE OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 5.070e-45
Identity = 95/109 (87.16%), Postives = 103/109 (94.50%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMSVSL+QLKTPELLY+SLAAKLIVGMPFKDLATVDSILL+ELPP+DD+D+RLALKRLVDISMGVI PLSEQLTKPLPNA+VLV LKELS GA KLLP+GTRL VS
Sbjct:  412 GSVLMSVSLNQLKTPELLYRSLAAKLIVGMPFKDLATVDSILLRELPPLDDIDSRLALKRLVDISMGVIAPLSEQLTKPLPNAMVLVTLKELSGGAHKLLPEGTRLVVS 520
[Close]
BLAST of AM161949 vs. TrEMBL
Match: Q84XR5_CATRO (GCPE protein OS=Catharanthus roseus PE=2 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 5.070e-45
Identity = 94/109 (86.24%), Postives = 103/109 (94.50%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMSVSLDQLKTPELLYKSLAAKL+VGMPFKDLATVDSILL+ELPP++D D+RLALKRL+DISMGVI PLSEQLTKPLPNA+VLV LKELS+GA KLLP+GTRL VS
Sbjct:  412 GSVLMSVSLDQLKTPELLYKSLAAKLVVGMPFKDLATVDSILLRELPPIEDKDSRLALKRLIDISMGVITPLSEQLTKPLPNAMVLVTLKELSSGAHKLLPEGTRLVVS 520
[Close]
BLAST of AM161949 vs. TrEMBL
Match: B9SWB7_RICCO (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, putative OS=Ricinus communis GN=RCOM_0154370 PE=3 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 5.070e-45
Identity = 95/109 (87.16%), Postives = 101/109 (92.66%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMSVSLDQLK PELLYKSLAAKL+VGMPFKDLATVDSILL+ELPP +D DARLALKRL+DISMGVI PLSEQLTKPLPNA+VLVNLKELS GA KLLP+GTRL VS
Sbjct:  414 GSVLMSVSLDQLKVPELLYKSLAAKLVVGMPFKDLATVDSILLRELPPAEDSDARLALKRLIDISMGVITPLSEQLTKPLPNAMVLVNLKELSTGAHKLLPEGTRLVVS 522
[Close]
BLAST of AM161949 vs. TrEMBL
Match: D7SKY1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00025182001 PE=4 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 1.130e-44
Identity = 94/109 (86.24%), Postives = 100/109 (91.74%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMSVSLDQLKTPEL YKSLAAKL++GMPFKDLATVDSILL+ELPPVDD DARL LKRLVD+SMGVI PLSEQLTKPLPNA+ LVNLKELS GA KLLP+GTRL VS
Sbjct:  412 GSVLMSVSLDQLKTPELFYKSLAAKLVIGMPFKDLATVDSILLRELPPVDDNDARLTLKRLVDVSMGVITPLSEQLTKPLPNAMALVNLKELSTGAYKLLPEGTRLVVS 520
[Close]
BLAST of AM161949 vs. TrEMBL
Match: A5BBY6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005654 PE=3 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 1.130e-44
Identity = 94/109 (86.24%), Postives = 100/109 (91.74%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMSVSLDQLKTPEL YKSLAAKL++GMPFKDLATVDSILL+ELPPVDD DARL LKRLVD+SMGVI PLSEQLTKPLPNA+ LVNLKELS GA KLLP+GTRL VS
Sbjct:  412 GSVLMSVSLDQLKTPELFYKSLAAKLVIGMPFKDLATVDSILLRELPPVDDNDARLTLKRLVDVSMGVITPLSEQLTKPLPNAMALVNLKELSTGAYKLLPEGTRLVVS 520
[Close]
BLAST of AM161949 vs. TrEMBL
Match: B3GEM5_POPTR (Putative chloroplast 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (Fragment) OS=Populus trichocarpa GN=HDS PE=2 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 1.475e-44
Identity = 94/109 (86.24%), Postives = 101/109 (92.66%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMSVSLDQLK PELLYKSLAAKL+VGMPFKDLATVDSILL+ELPPVDD D RLALKRL+++SMGVI PLSEQLTKPLPNA+VLVNLKELS GA KLLP+GTRL VS
Sbjct:  351 GSVLMSVSLDQLKAPELLYKSLAAKLVVGMPFKDLATVDSILLRELPPVDDNDVRLALKRLIEVSMGVIAPLSEQLTKPLPNAMVLVNLKELSTGAYKLLPEGTRLVVS 459
[Close]
BLAST of AM161949 vs. TrEMBL
Match: B9MZ45_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_836719 PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.516e-44
Identity = 93/109 (85.32%), Postives = 101/109 (92.66%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMSVSLDQLK PELLYKSLAAK++VGMPFKDLATVDSI L+ELPPVDD DARLALKRL+++SMGVI PLSEQLTKPLPNA+VLVNLKELS GA KLLP+GTRL VS
Sbjct:  432 GSVLMSVSLDQLKAPELLYKSLAAKIVVGMPFKDLATVDSIFLRELPPVDDNDARLALKRLIEVSMGVIAPLSEQLTKPLPNAMVLVNLKELSTGAYKLLPEGTRLVVS 540
[Close]
BLAST of AM161949 vs. TrEMBL
Match: B9HQ97_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_821630 PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.516e-44
Identity = 93/109 (85.32%), Postives = 101/109 (92.66%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMSVSLDQLK PELLYKSLAAK++VGMPFKDLATVDSI L+ELPPVDD DARLALKRL+++SMGVI PLSEQLTKPLPNA+VLVNLKELS GA KLLP+GTRL VS
Sbjct:  412 GSVLMSVSLDQLKAPELLYKSLAAKIVVGMPFKDLATVDSIFLRELPPVDDNDARLALKRLIEVSMGVIAPLSEQLTKPLPNAMVLVNLKELSTGAYKLLPEGTRLVVS 520
[Close]
BLAST of AM161949 vs. TrEMBL
Match: D2D5E3_ARTAN ((E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase OS=Artemisia annua GN=HDS PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 7.322e-44
Identity = 90/109 (82.57%), Postives = 100/109 (91.74%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G V+MSV+LDQLKTPEL Y+SLAAKL++GMPFKDLATVDSILL+ELPP DD DARLALKRL+D+SMG+I PLSEQLTKPLPNAIVLVNL ELS GA KLLP+GTRL VS
Sbjct:  289 GSVIMSVTLDQLKTPELFYRSLAAKLVLGMPFKDLATVDSILLRELPPADDKDARLALKRLIDVSMGIITPLSEQLTKPLPNAIVLVNLNELSTGAHKLLPEGTRLVVS 397
[Close]
BLAST of AM161949 vs. TAIR peptide
Match: AT5G60600.3 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363019 FORWARD LENGTH=717)

HSP 1 Score: 179.104 bits (453), Expect = 4.830e-46
Identity = 92/109 (84.40%), Postives = 100/109 (91.74%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMS+SLDQLK PELLY+SLA KL+VGMPFKDLATVDSILL+ELPPVDD  ARLALKRL+D+SMGVI PLSEQLTKPLPNA+VLVNLKELS GA KLLP+GTRL VS
Sbjct:  412 GSVLMSISLDQLKAPELLYRSLATKLVVGMPFKDLATVDSILLRELPPVDDQVARLALKRLIDVSMGVIAPLSEQLTKPLPNAMVLVNLKELSGGAYKLLPEGTRLVVS 520
[Close]
BLAST of AM161949 vs. TAIR peptide
Match: AT5G60600.2 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363274 FORWARD LENGTH=740)

HSP 1 Score: 179.104 bits (453), Expect = 4.830e-46
Identity = 92/109 (84.40%), Postives = 100/109 (91.74%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMS+SLDQLK PELLY+SLA KL+VGMPFKDLATVDSILL+ELPPVDD  ARLALKRL+D+SMGVI PLSEQLTKPLPNA+VLVNLKELS GA KLLP+GTRL VS
Sbjct:  411 GSVLMSISLDQLKAPELLYRSLATKLVVGMPFKDLATVDSILLRELPPVDDQVARLALKRLIDVSMGVIAPLSEQLTKPLPNAMVLVNLKELSGGAYKLLPEGTRLVVS 519
[Close]
BLAST of AM161949 vs. TAIR peptide
Match: AT5G60600.1 (| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy-3-methylbut-2-enyl diphosphate synthase | chr5:24359447-24363274 FORWARD LENGTH=741)

HSP 1 Score: 179.104 bits (453), Expect = 4.830e-46
Identity = 92/109 (84.40%), Postives = 100/109 (91.74%), Query Frame = 2 
Query:   17 GLVLMSVSLDQLKTPELLYKSLAAKLIVGMPFKDLATVDSILLQELPPVDDVDARLALKRLVDISMGVIVPLSEQLTKPLPNAIVLVNLKELSAGADKLLPQGTRLAVS 343
            G VLMS+SLDQLK PELLY+SLA KL+VGMPFKDLATVDSILL+ELPPVDD  ARLALKRL+D+SMGVI PLSEQLTKPLPNA+VLVNLKELS GA KLLP+GTRL VS
Sbjct:  412 GSVLMSISLDQLKAPELLYRSLATKLVVGMPFKDLATVDSILLRELPPVDDQVARLALKRLIDVSMGVIAPLSEQLTKPLPNAMVLVNLKELSGGAYKLLPEGTRLVVS 520
The following BLAST results are available for this feature:
BLAST of AM161949 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
A9ZN14_HEVBR2.973e-4588.074-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
Q8GZR6_SOLLC5.070e-4587.16GcpE OS=Solanum lycopersicum PE=2 SV=1[more]
Q84XR5_CATRO5.070e-4586.24GCPE protein OS=Catharanthus roseus PE=2 SV=1[more]
B9SWB7_RICCO5.070e-4587.164-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
D7SKY1_VITVI1.130e-4486.24Whole genome shotgun sequence of line PN40024, sca... [more]
A5BBY6_VITVI1.130e-4486.24Putative uncharacterized protein OS=Vitis vinifera... [more]
B3GEM5_POPTR1.475e-4486.24Putative chloroplast 4-hydroxy-3-methylbut-2-en-1-... [more]
B9MZ45_POPTR2.516e-4485.32Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9HQ97_POPTR2.516e-4485.32Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D2D5E3_ARTAN7.322e-4482.57(E)-4-hydroxy-3-methylbut-2-enyl diphosphate synth... [more]
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BLAST of AM161949 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT5G60600.34.830e-4684.40| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy... [more]
AT5G60600.24.830e-4684.40| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy... [more]
AT5G60600.14.830e-4684.40| Symbols: GCPE, ISPG, CSB3, CLB4, HDS | 4-hydroxy... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM161949 ID=AM161949; Name=AM161949; organism=Pisum sativum; type=EST; length=345bp
ACACTCCACCGGGACGGGTCTTGTTCTTATGTCAGTCTCCTTGGATCAGT
TAAAGACACCAGAACTCCTTTACAAGTCACTTGCTGCGAAACTCATTGTT
GGCATGCCATTTAAGGATCTGGCAACAGTAGATTCAATCTTATTGCAGGA
ACTTCCTCCGGTAGATGATGTTGATGCTAGGTTAGCTCTGAAAAGACTGG
TTGATATAAGTATGGGAGTTATAGTTCCTTTGTCAGAGCAGCTAACGAAG
CCATTACCAAATGCCATAGTTCTGGTAAACCTTAAGGAGCTATCGGCTGG
AGCTGATAAGCTTTTGCCTCAAGGCACACGATTGGCTGTGTCAGT
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