AM161956

EST Overview


Name	AM161956
Unique Name	AM161956
Type	EST
Organism	Pisum sativum (pea)
Sequence length	360

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM161956 vs. TrEMBL
Match: Q2MGR0_MEDTR (ParB-like nuclease OS=Medicago truncatula GN=MtrDRAFT_AC147481g19v2 PE=4 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 3.551e-38
Identity = 76/81 (93.83%), Postives = 80/81 (98.77%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGPVI+ELPLDKIRRP+MRTRSNDQ KVQELMDSI++IGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG
Sbjct:   35 GVGPVILELPLDKIRRPMMRTRSNDQNKVQELMDSISEIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 115

[Close]

BLAST of AM161956 vs. TrEMBL
Match: D7KKA2_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_473615 PE=4 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 4.341e-36
Identity = 71/81 (87.65%), Postives = 78/81 (96.30%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGP+I+ELPLDKIRRPLMRTRSNDQ KV+ELMDSI QIGLQVP+DV+EVDG YYGFSGCHRYEAHQ+LGLPTIRCKIR+G
Sbjct:   35 GVGPMIVELPLDKIRRPLMRTRSNDQNKVKELMDSIRQIGLQVPVDVIEVDGTYYGFSGCHRYEAHQKLGLPTIRCKIRKG 115

[Close]

BLAST of AM161956 vs. TrEMBL
Match: Q9SA12_ARATH (F28K20.12 OS=Arabidopsis thaliana GN=F28K20.12 PE=4 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 9.670e-36
Identity = 71/81 (87.65%), Postives = 78/81 (96.30%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGP+I+ELPL+KIRRPLMRTRSNDQ KV+ELMDSI QIGLQVPIDV+EVDG YYGFSGCHRYEAHQ+LGLPTIRCKIR+G
Sbjct:   35 GVGPMIVELPLEKIRRPLMRTRSNDQNKVKELMDSIRQIGLQVPIDVIEVDGTYYGFSGCHRYEAHQKLGLPTIRCKIRKG 115

[Close]

BLAST of AM161956 vs. TrEMBL
Match: Q8GY89_ARATH (At1g31170 OS=Arabidopsis thaliana GN=At1g31170/F28K20_12 PE=2 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 9.670e-36
Identity = 71/81 (87.65%), Postives = 78/81 (96.30%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGP+I+ELPL+KIRRPLMRTRSNDQ KV+ELMDSI QIGLQVPIDV+EVDG YYGFSGCHRYEAHQ+LGLPTIRCKIR+G
Sbjct:   35 GVGPMIVELPLEKIRRPLMRTRSNDQNKVKELMDSIRQIGLQVPIDVIEVDGTYYGFSGCHRYEAHQKLGLPTIRCKIRKG 115

[Close]

BLAST of AM161956 vs. TrEMBL
Match: D2KKL9_PEA (Sulfiredoxin protein OS=Pisum sativum GN=Srx PE=4 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 9.670e-36
Identity = 71/81 (87.65%), Postives = 78/81 (96.30%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGP+I+ELPL+KIRRPLMRTRSNDQ KV+ELMDSI QIGLQVPIDV+EVDG YYGFSGCHRYEAHQ+LGLPTIRCKIR+G
Sbjct:   39 GVGPMIVELPLEKIRRPLMRTRSNDQNKVKELMDSIRQIGLQVPIDVIEVDGTYYGFSGCHRYEAHQKLGLPTIRCKIRKG 119

[Close]

BLAST of AM161956 vs. TrEMBL
Match: Q8LC51_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.154e-35
Identity = 70/81 (86.42%), Postives = 78/81 (96.30%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGP+I+ELPL+KIRRPLMRT+SNDQ KV+ELMDSI QIGLQVPIDV+EVDG YYGFSGCHRYEAHQ+LGLPTIRCKIR+G
Sbjct:   35 GVGPMIVELPLEKIRRPLMRTKSNDQNKVKELMDSIRQIGLQVPIDVIEVDGTYYGFSGCHRYEAHQKLGLPTIRCKIRKG 115

[Close]

BLAST of AM161956 vs. TrEMBL
Match: C6T4A5_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 3.675e-35
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            G GPVI+ELPLDKIRRPLMRTR+NDQ KV ELMDSI +IGLQVPIDVLEVDG YYGFSGCHRYEAHQRLGLPTIRCKIRRG
Sbjct:   38 GGGPVILELPLDKIRRPLMRTRTNDQNKVLELMDSIKEIGLQVPIDVLEVDGVYYGFSGCHRYEAHQRLGLPTIRCKIRRG 118

[Close]

BLAST of AM161956 vs. TrEMBL
Match: B9SCC3_RICCO (Sulfiredoxin-1, putative OS=Ricinus communis GN=RCOM_0891620 PE=4 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 3.675e-35
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = -1

Query:    1 GPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 237
            GPVI+ELPLDKIRRPLMRTR+ND  KVQ+LMDSI QIGLQVPIDVLEVDG YYGFSGCHRYEAHQRLGLPTIRCK+RRG
Sbjct:   39 GPVIVELPLDKIRRPLMRTRANDPTKVQDLMDSIEQIGLQVPIDVLEVDGVYYGFSGCHRYEAHQRLGLPTIRCKVRRG 117

[Close]

BLAST of AM161956 vs. TrEMBL
Match: B9I4P9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_814302 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.824e-34
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = -1

Query:    1 GPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 237
            GPVI+ELPLDKIRRPLMRTR+ND  KV+ELMDSI +IGLQVPIDVLEVDG YYGFSGCHRYEAHQRLGLPTIRCKIRRG
Sbjct:   37 GPVILELPLDKIRRPLMRTRANDPNKVKELMDSIKEIGLQVPIDVLEVDGVYYGFSGCHRYEAHQRLGLPTIRCKIRRG 115

[Close]

BLAST of AM161956 vs. TrEMBL
Match: C5Z5B8_SORBI (Putative uncharacterized protein Sb10g005040 OS=Sorghum bicolor GN=Sb10g005040 PE=4 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.111e-34
Identity = 69/79 (87.34%), Postives = 75/79 (94.94%), Query Frame = -1

Query:    1 GPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 237
            GPVI+E+PLD+IRRPLMRTR+ND +KVQELMDSI  IGLQVPIDVLEVDG YYGFSGCHRYEAHQRLGLPTIRCK+RRG
Sbjct:   66 GPVIMEIPLDQIRRPLMRTRANDPVKVQELMDSIRVIGLQVPIDVLEVDGVYYGFSGCHRYEAHQRLGLPTIRCKVRRG 144

[Close]

BLAST of AM161956 vs. SwissProt
Match: LVSE_DICDI (BEACH domain-containing protein lvsE OS=Dictyostelium discoideum GN=lvsE PE=4 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.339e-7
Identity = 35/80 (43.75%), Postives = 47/80 (58.75%), Query Frame = -1

Query:   10 IIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVP-IDVLEVDGN------YYGFSGCHRYEAHQRLGLPTIRCKI 228
            + E+P+  I RPL      D+ KV  LM++I   G+++P IDV  V G       Y+ F GCHRYEA +RL L TIR +I
Sbjct:   10 VTEMPMSVIHRPL--PSELDEEKVLSLMETIKS-GVEIPPIDVNWVKGKDENNNYYFSFGGCHRYEATKRLNLKTIRARI 86

[Close]

BLAST of AM161956 vs. SwissProt
Match: SRX_DROME (Putative sulfiredoxin OS=Drosophila melanogaster GN=CG6762 PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 5.090e-7
Identity = 31/78 (39.74%), Postives = 47/78 (60.26%), Query Frame = -1

Query:   10 LPLDKIRRPLMRTRSNDQIKVQELMDSI---TQIGLQVPIDVLEVDGN-----YYGFSGCHRYEAHQRLGLPTIRCKI 219
            +P+  I+RP+      D+ KVQ LM++I   T      PID+L + G+     Y+ F GCHR+EA++RL  PTI+ K+
Sbjct:   66 VPMSVIQRPIPSVL--DEQKVQSLMETIKNETSEDEVPPIDLLWISGSEGGDYYFSFGGCHRFEAYKRLQRPTIKAKL 141

[Close]

BLAST of AM161956 vs. TAIR peptide
Match: AT1G31170.4 (| Symbols: SRX | sulfiredoxin | chr1:11133521-11134686 FORWARD LENGTH=198)

HSP 1 Score: 152.91 bits (385), Expect = 3.898e-38
Identity = 71/81 (87.65%), Postives = 78/81 (96.30%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGP+I+ELPL+KIRRPLMRTRSNDQ KV+ELMDSI QIGLQVPIDV+EVDG YYGFSGCHRYEAHQ+LGLPTIRCKIR+G
Sbjct:   35 GVGPMIVELPLEKIRRPLMRTRSNDQNKVKELMDSIRQIGLQVPIDVIEVDGTYYGFSGCHRYEAHQKLGLPTIRCKIRKG 115

[Close]

BLAST of AM161956 vs. TAIR peptide
Match: AT1G31170.2 (| Symbols: ATSRX, SRX | sulfiredoxin | chr1:11133521-11134228 FORWARD LENGTH=125)

HSP 1 Score: 152.91 bits (385), Expect = 3.898e-38
Identity = 71/81 (87.65%), Postives = 78/81 (96.30%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGP+I+ELPL+KIRRPLMRTRSNDQ KV+ELMDSI QIGLQVPIDV+EVDG YYGFSGCHRYEAHQ+LGLPTIRCKIR+G
Sbjct:   35 GVGPMIVELPLEKIRRPLMRTRSNDQNKVKELMDSIRQIGLQVPIDVIEVDGTYYGFSGCHRYEAHQKLGLPTIRCKIRKG 115

[Close]

BLAST of AM161956 vs. TAIR peptide
Match: AT1G31170.1 (| Symbols: ATSRX, SRX | sulfiredoxin | chr1:11133521-11134228 FORWARD LENGTH=125)

HSP 1 Score: 152.91 bits (385), Expect = 3.898e-38
Identity = 71/81 (87.65%), Postives = 78/81 (96.30%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGP+I+ELPL+KIRRPLMRTRSNDQ KV+ELMDSI QIGLQVPIDV+EVDG YYGFSGCHRYEAHQ+LGLPTIRCKIR+G
Sbjct:   35 GVGPMIVELPLEKIRRPLMRTRSNDQNKVKELMDSIRQIGLQVPIDVIEVDGTYYGFSGCHRYEAHQKLGLPTIRCKIRKG 115

[Close]

BLAST of AM161956 vs. TAIR peptide
Match: AT1G31170.3 (| Symbols: ATSRX, SRX | sulfiredoxin | chr1:11133521-11134228 FORWARD LENGTH=123)

HSP 1 Score: 143.665 bits (361), Expect = 2.365e-35
Identity = 69/81 (85.19%), Postives = 76/81 (93.83%), Query Frame = -1

Query:    1 GVGPVIIELPLDKIRRPLMRTRSNDQIKVQELMDSITQIGLQVPIDVLEVDGNYYGFSGCHRYEAHQRLGLPTIRCKIRRG 243
            GVGP+I+ELPL+KIRRPLMRTRSNDQ KV+ELMDSI QIGLQ  IDV+EVDG YYGFSGCHRYEAHQ+LGLPTIRCKIR+G
Sbjct:   35 GVGPMIVELPLEKIRRPLMRTRSNDQNKVKELMDSIRQIGLQ--IDVIEVDGTYYGFSGCHRYEAHQKLGLPTIRCKIRKG 113

The following BLAST results are available for this feature:

BLAST of AM161956 vs. TrEMBL
BLAST of AM161956 vs. SwissProt
BLAST of AM161956 vs. TAIR peptide

BLAST of AM161956 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q2MGR0_MEDTR	3.551e-38	93.83	ParB-like nuclease OS=Medicago truncatula GN=MtrDR...	[more]
D7KKA2_ARALY	4.341e-36	87.65	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9SA12_ARATH	9.670e-36	87.65	F28K20.12 OS=Arabidopsis thaliana GN=F28K20.12 PE=...	[more]
Q8GY89_ARATH	9.670e-36	87.65	At1g31170 OS=Arabidopsis thaliana GN=At1g31170/F28...	[more]
D2KKL9_PEA	9.670e-36	87.65	Sulfiredoxin protein OS=Pisum sativum GN=Srx PE=4 ...	[more]
Q8LC51_ARATH	2.154e-35	86.42	Putative uncharacterized protein OS=Arabidopsis th...	[more]
C6T4A5_SOYBN	3.675e-35	90.12	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9SCC3_RICCO	3.675e-35	89.87	Sulfiredoxin-1, putative OS=Ricinus communis GN=RC...	[more]
B9I4P9_POPTR	1.824e-34	89.87	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C5Z5B8_SORBI	3.111e-34	87.34	Putative uncharacterized protein Sb10g005040 OS=So...	[more]

BLAST of AM161956 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
LVSE_DICDI	1.339e-7	43.75	BEACH domain-containing protein lvsE OS=Dictyostel...	[more]
SRX_DROME	5.090e-7	39.74	Putative sulfiredoxin OS=Drosophila melanogaster G...	[more]

BLAST of AM161956 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G31170.4	3.898e-38	87.65	\| Symbols: SRX \| sulfiredoxin \| chr1:11133521-1113...	[more]
AT1G31170.2	3.898e-38	87.65	\| Symbols: ATSRX, SRX \| sulfiredoxin \| chr1:111335...	[more]
AT1G31170.1	3.898e-38	87.65	\| Symbols: ATSRX, SRX \| sulfiredoxin \| chr1:111335...	[more]
AT1G31170.3	2.365e-35	85.19	\| Symbols: ATSRX, SRX \| sulfiredoxin \| chr1:111335...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003115	ParB-like nuclease	PFAM	PF02195	ParBc	coord: 36..107 score: 1.4
IPR003115	ParB-like nuclease	SMART	SM00470	no description	coord: 35..109 score: 2.4
IPR003115	ParB-like nuclease	SUPERFAMILY	110849	ParB/Sulfiredoxin	coord: 34..107 score: 5.8
IPR016692	Sulfiredoxin	PIR	PIRSF017267	Sulfiredoxin	coord: 6..109 score: 2.5
None	No IPR available	GENE3D	3.90.1530.10	no description	coord: 32..107 score: 1
None	No IPR available	PANTHER	PTHR21348	UNCHARACTERIZED	coord: 29..106 score: 1.5
None	No IPR available	SEG	seg	seg	coord: 10..26 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM161956 ID=AM161956; Name=AM161956; organism=Pisum sativum; type=EST; length=360bp
ACCACGCCGTATTTTACAGCGTATGGTAGGGAGTCCAAGTCGTTGGTGAG
CCTCATAGCGGTGACAGCCGGAGAAACCATAATAGTTTCCATCCACCTCG
AGCACATCGATAGGCACTTGAAGACCAATCTGAGTAATACTATCCATGAG
TTCTTGAACTTTGATCTGATCATTTGATCTAGTCCTCATCAGAGGTCTTC
TTATCTTATCCAAAGGAAGCTCAATGATCACTGGACCCACACCCTTATCA
GAAGAACTGCTTCCAGAAACAGCCCCGTTGGAGTTGGATGAAGCAGACAC
ATGAAAACTCCTCAAAGGAATTGGAACCTAGCAAAACAAAATTTGCCATT
TTTTTCTTTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0032542	sulfiredoxin activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR003115	ParB/Sulfiredoxin_dom
IPR016692	Sulfiredoxin

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