CD860274

EST Overview


Name	CD860274
Unique Name	CD860274
Type	EST
Organism	Pisum sativum (pea)
Sequence length	412

Libraries


Library Name	Type
LIBEST_014002 PW	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD860274 vs. TrEMBL
Match: C6TIA6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.318e-12
Identity = 35/42 (83.33%), Postives = 39/42 (92.86%), Query Frame = -2

Query:  286 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSGFEDSLEQKQQ 411
            KSGNYSSTFTYMDRIFG+D+GYRKLKALK+ G EDS EQK+Q
Sbjct:  230 KSGNYSSTFTYMDRIFGTDIGYRKLKALKSIGVEDSGEQKKQ 271

[Close]

BLAST of CD860274 vs. TrEMBL
Match: C6TM45_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.334e-12
Identity = 34/42 (80.95%), Postives = 39/42 (92.86%), Query Frame = -2

Query:  286 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSGFEDSLEQKQQ 411
            KSGNYSSTFTYMDRIFG+D+GYRKLKALK+ G ED+ EQK+Q
Sbjct:  230 KSGNYSSTFTYMDRIFGTDIGYRKLKALKSIGVEDNSEQKKQ 271

[Close]

BLAST of CD860274 vs. TrEMBL
Match: B9S3D6_RICCO (C-4 methyl sterol oxidase, putative OS=Ricinus communis GN=RCOM_0732630 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 9.992e-9
Identity = 30/39 (76.92%), Postives = 34/39 (87.18%), Query Frame = -2

Query:  295 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSGFEDSLEQ 411
            KSGNYSSTF YMD IFG+DVGYRKLKALK+SG E+  +Q
Sbjct:  230 KSGNYSSTFIYMDWIFGTDVGYRKLKALKSSGVENGNKQ 268

[Close]

BLAST of CD860274 vs. TrEMBL
Match: B9HPC4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_723203 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.907e-8
Identity = 28/39 (71.79%), Postives = 34/39 (87.18%), Query Frame = -2

Query:  295 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSGFEDSLEQ 411
            KSGNYSSTFTYMD +FG+D GYRKL+ALKN+G E+  +Q
Sbjct:  230 KSGNYSSTFTYMDWVFGTDKGYRKLQALKNAGVENGGKQ 268

[Close]

BLAST of CD860274 vs. TrEMBL
Match: B9GJ78_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_752102 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.907e-8
Identity = 28/39 (71.79%), Postives = 34/39 (87.18%), Query Frame = -2

Query:  295 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSGFEDSLEQ 411
            KSGNYSSTFTYMD +FG+D GYRKL+ALKN+G E+  +Q
Sbjct:  230 KSGNYSSTFTYMDWVFGTDKGYRKLQALKNAGVENGGKQ 268

[Close]

BLAST of CD860274 vs. TrEMBL
Match: A9P9T0_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.907e-8
Identity = 28/39 (71.79%), Postives = 34/39 (87.18%), Query Frame = -2

Query:  295 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSGFEDSLEQ 411
            KSGNYSSTFTYMD +FG+D GYRKL+ALKN+G E+  +Q
Sbjct:  230 KSGNYSSTFTYMDWVFGTDKGYRKLQALKNAGVENGGKQ 268

[Close]

BLAST of CD860274 vs. TrEMBL
Match: Q8GTA1_GOSAR (Putative sterol 4-alpha-methyl-oxidase OS=Gossypium arboreum PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 6.476e-8
Identity = 28/39 (71.79%), Postives = 31/39 (79.49%), Query Frame = -2

Query:  295 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSGFEDSLEQ 411
            KSGNYSSTF YMD IFG+D GYRKLKALK  G E+  +Q
Sbjct:  230 KSGNYSSTFVYMDWIFGTDKGYRKLKALKRDGVEEEAKQ 268

[Close]

BLAST of CD860274 vs. TrEMBL
Match: A9RVU6_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_205949 PE=4 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 7.160e-7
Identity = 29/41 (70.73%), Postives = 34/41 (82.93%), Query Frame = -2

Query:  292 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSGFE-DSLEQK 411
            KSGNYSSTFTYMD IFG+D GYRKLKALK +  E + +E+K
Sbjct:  230 KSGNYSSTFTYMDWIFGTDKGYRKLKALKAADHEYEYVEEK 270

[Close]

BLAST of CD860274 vs. TAIR peptide
Match: AT2G29390.5 (| Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-2 | chr2:12610758-12611929 REVERSE LENGTH=219)

HSP 1 Score: 54.299 bits (129), Expect = 2.662e-8
Identity = 25/31 (80.65%), Postives = 27/31 (87.10%), Query Frame = -2

Query:  319 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNS 411
            KSGNYSSTF YMD IFG+D GYRKLKALK +
Sbjct:  189 KSGNYSSTFVYMDWIFGTDKGYRKLKALKET 219

[Close]

BLAST of CD860274 vs. TAIR peptide
Match: AT2G29390.4 (| Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-2 | chr2:12610758-12611731 REVERSE LENGTH=177)

HSP 1 Score: 54.299 bits (129), Expect = 2.662e-8
Identity = 25/31 (80.65%), Postives = 27/31 (87.10%), Query Frame = -2

Query:  319 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNS 411
            KSGNYSSTF YMD IFG+D GYRKLKALK +
Sbjct:  147 KSGNYSSTFVYMDWIFGTDKGYRKLKALKET 177

[Close]

BLAST of CD860274 vs. TAIR peptide
Match: AT2G29390.2 (| Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-2 | chr2:12610758-12612799 REVERSE LENGTH=260)

HSP 1 Score: 54.299 bits (129), Expect = 2.662e-8
Identity = 25/31 (80.65%), Postives = 27/31 (87.10%), Query Frame = -2

Query:  319 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNS 411
            KSGNYSSTF YMD IFG+D GYRKLKALK +
Sbjct:  230 KSGNYSSTFVYMDWIFGTDKGYRKLKALKET 260

[Close]

BLAST of CD860274 vs. TAIR peptide
Match: AT2G29390.3 (| Symbols: ATSMO2 | sterol 4-alpha-methyl-oxidase 2-2 | chr2:12610758-12612149 REVERSE LENGTH=253)

HSP 1 Score: 54.299 bits (129), Expect = 2.662e-8
Identity = 25/31 (80.65%), Postives = 27/31 (87.10%), Query Frame = -2

Query:  319 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNS 411
            KSGNYSSTF YMD IFG+D GYRKLKALK +
Sbjct:  223 KSGNYSSTFVYMDWIFGTDKGYRKLKALKET 253

[Close]

BLAST of CD860274 vs. TAIR peptide
Match: AT2G29390.1 (| Symbols: SMO2-2, ATSMO2 | sterol 4-alpha-methyl-oxidase 2-2 | chr2:12610758-12612799 REVERSE LENGTH=272)

HSP 1 Score: 54.299 bits (129), Expect = 2.662e-8
Identity = 25/31 (80.65%), Postives = 27/31 (87.10%), Query Frame = -2

Query:  319 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNS 411
            KSGNYSSTF YMD IFG+D GYRKLKALK +
Sbjct:  242 KSGNYSSTFVYMDWIFGTDKGYRKLKALKET 272

[Close]

BLAST of CD860274 vs. TAIR peptide
Match: AT1G07420.2 (| Symbols: ATSMO2, SMO2-1 | sterol 4-alpha-methyl-oxidase 2-1 | chr1:2278918-2280033 FORWARD LENGTH=228)

HSP 1 Score: 53.9138 bits (128), Expect = 3.477e-8
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = -2

Query:  316 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSG 411
            KSGNYSSTF YMD IFG+D GYR+LK LK +G
Sbjct:  192 KSGNYSSTFVYMDWIFGTDKGYRRLKTLKENG 223

[Close]

BLAST of CD860274 vs. TAIR peptide
Match: AT1G07420.1 (| Symbols: ATSMO2, SMO2-1 | sterol 4-alpha-methyl-oxidase 2-1 | chr1:2277910-2280033 FORWARD LENGTH=266)

HSP 1 Score: 53.9138 bits (128), Expect = 3.477e-8
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = -2

Query:  316 KSGNYSSTFTYMDRIFGSDVGYRKLKALKNSG 411
            KSGNYSSTF YMD IFG+D GYR+LK LK +G
Sbjct:  230 KSGNYSSTFVYMDWIFGTDKGYRRLKTLKENG 261

The following BLAST results are available for this feature:

BLAST of CD860274 vs. TrEMBL
BLAST of CD860274 vs. TAIR peptide

BLAST of CD860274 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 8


Match Name	E-value	Identity	Description
C6TIA6_SOYBN	3.318e-12	83.33	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6TM45_SOYBN	4.334e-12	80.95	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9S3D6_RICCO	9.992e-9	76.92	C-4 methyl sterol oxidase, putative OS=Ricinus com...	[more]
B9HPC4_POPTR	2.907e-8	71.79	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9GJ78_POPTR	2.907e-8	71.79	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9P9T0_POPTR	2.907e-8	71.79	Putative uncharacterized protein OS=Populus tricho...	[more]
Q8GTA1_GOSAR	6.476e-8	71.79	Putative sterol 4-alpha-methyl-oxidase OS=Gossypiu...	[more]
A9RVU6_PHYPA	7.160e-7	70.73	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of CD860274 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT2G29390.5	2.662e-8	80.65	\| Symbols: ATSMO2 \| sterol 4-alpha-methyl-oxidase ...	[more]
AT2G29390.4	2.662e-8	80.65	\| Symbols: ATSMO2 \| sterol 4-alpha-methyl-oxidase ...	[more]
AT2G29390.2	2.662e-8	80.65	\| Symbols: ATSMO2 \| sterol 4-alpha-methyl-oxidase ...	[more]
AT2G29390.3	2.662e-8	80.65	\| Symbols: ATSMO2 \| sterol 4-alpha-methyl-oxidase ...	[more]
AT2G29390.1	2.662e-8	80.65	\| Symbols: SMO2-2, ATSMO2 \| sterol 4-alpha-methyl-...	[more]
AT1G07420.2	3.477e-8	75.00	\| Symbols: ATSMO2, SMO2-1 \| sterol 4-alpha-methyl-...	[more]
AT1G07420.1	3.477e-8	75.00	\| Symbols: ATSMO2, SMO2-1 \| sterol 4-alpha-methyl-...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 58..65 score
None	No IPR available	SEG	seg	seg	coord: 9..21 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD860274 ID=CD860274; Name=CD860274; organism=Pisum sativum; type=EST; length=412bp
ATCCAAATTGTAATATCAATTGGATTTATATCAAACAAATAACAGTCATT
ACATGAAGAGGTATCACAATTTTCAAAGTAGCGTAGCTAAAAGCATTTTG
AATAACAAGCATCAATGTCGATAAAAAACCATCAACACTCACTTTCTCAA
CAAAAGAGCATCTACAACAAAAAAAAACACAACTACAATGTGTTGATAGA
AAAGATAATTTTCAAATATACTCTCAAGTTAGGATTGGGAATCAACTTTC
TAACCACGCCAGATGTTCCCAAAAATTTGTTCTTATTGCTGCTTTTGCTC
GAGACTGTCTTCAAACCCTGAGTTCTTCAATGCTTTTAACTTTCTGTATC
CTACATCACTTCCAAATATCCGGTCCATGTAAGTGAAAGTTGATGAATAG
TTTCCGGACTTG

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