EX569786

EST Overview


Name	EX569786
Unique Name	EX569786
Type	EST
Organism	Pisum sativum (pea)
Sequence length	509

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of EX569786 vs. TrEMBL
Match: C6T9F1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 2.493e-63
Identity = 116/171 (67.84%), Postives = 139/171 (81.29%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVE--ANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN IDLSN +I QSVNLLK++C DSGFFY+VNHGISQEFMD+VF QS+KFF+LP NEKMK LRN+  RGYTP+LDE+LDP+NQ+HGD+KEGYYIGVE   +D    KPF+GPN WPA DVLPGWRETM ++ ++ LEVG+AVAK          N+FD+PE LG+PIAILR
Sbjct:   12 LNCIDLSNPDINQSVNLLKEACLDSGFFYVVNHGISQEFMDEVFAQSKKFFSLPHNEKMKTLRNEQHRGYTPVLDELLDPENQVHGDYKEGYYIGVEKGEDDPQSKKPFYGPNNWPAPDVLPGWRETMRKFHQETLEVGKAVAKMIALALDLDANYFDRPEILGEPIAILR 182

[Close]

BLAST of EX569786 vs. TrEMBL
Match: C6THB6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 1.368e-61
Identity = 116/172 (67.44%), Postives = 139/172 (80.81%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLH-GDFKEGYYIGVE--ANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN IDLSN +I QSVNLLKQ+C DSGFFY+VNHGISQEFM++VF QS+KFF+LP  EKMK+LRN+  RGYTP+LDE+LDP+NQ+H GD+KEGYYIGVE   +D   +KPF+GPN WPA  VLPGWRETME++ R+ LEVG+AVAK          NFFD+PE LG+PIA LR
Sbjct:   11 LNSIDLSNPDINQSVNLLKQACLDSGFFYVVNHGISQEFMEEVFAQSKKFFSLPHKEKMKILRNEKHRGYTPVLDELLDPENQVHVGDYKEGYYIGVEKGEDDPESNKPFYGPNNWPAPGVLPGWRETMEKFHRETLEVGKAVAKIIALALDLDANFFDQPEMLGEPIATLR 182

[Close]

BLAST of EX569786 vs. TrEMBL
Match: B9RKI5_RICCO (Hyoscyamine 6-dioxygenase, putative OS=Ricinus communis GN=RCOM_1049920 PE=3 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 1.672e-59
Identity = 106/171 (61.99%), Postives = 137/171 (80.12%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEA--NDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LNRIDLS+ +I QSV+LLKQ+C D GFFY++NHGIS+EFM++VF +S+KFF LP +EKMKVLRN+  RGYTP+LDE+LDP NQ+HGD+KEGYYIGVE   +D    KPF+GPN+WP  D+LPGWR+TME++ ++ALEV R +A+          NFFD+PE LG PIA++R
Sbjct:   21 LNRIDLSSSDIHQSVSLLKQACLDCGFFYVINHGISEEFMEEVFKESKKFFELPESEKMKVLRNEKHRGYTPVLDELLDPDNQVHGDYKEGYYIGVEVPEDDPEADKPFYGPNVWPKPDLLPGWRQTMERFHQEALEVARKIARIIALALDLDANFFDRPEMLGQPIAVMR 191

[Close]

BLAST of EX569786 vs. TrEMBL
Match: D7TBH3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_16.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00015310001 PE=4 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.184e-59
Identity = 109/171 (63.74%), Postives = 139/171 (81.29%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEA--NDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN IDLS+ +I +SV+LLKQ+C DSGFFY++NHGISQEFMD+VF QS++FF LPL+EKMK LRN+  RGYTP+LDE+LDP+NQ++GD+KEGYYIGVE   +D    KPF+GPN+WPA+D LP WR+TME++ ++ALEV +AVA+          +FFDKPE LGDPIA LR
Sbjct:   11 LNCIDLSSPDIHKSVSLLKQACLDSGFFYVINHGISQEFMDEVFAQSKRFFTLPLSEKMKHLRNEKHRGYTPVLDEVLDPENQVNGDYKEGYYIGVEVPEHDPKSEKPFYGPNVWPAADRLPEWRQTMEKFHQEALEVAKAVARIIALALDLERDFFDKPEMLGDPIATLR 181

[Close]

BLAST of EX569786 vs. TrEMBL
Match: B9ILT7_POPTR (2-oxoglutarate-dependent dioxygenase OS=Populus trichocarpa GN=2OGox16 PE=4 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 6.353e-59
Identity = 110/172 (63.95%), Postives = 136/172 (79.07%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLH-GDFKEGYYIGVEA--NDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN IDLS  ++QQSV+LLKQ+C D GFFY+ NHGISQEFM++VF QS+KFF LPL+EKMKVLRN+  RGYTP+LDE+LDP NQ+H GD+KEGYYIGVE   +D    KPF+GPN+WPA  +LPGWR+TME++ +QAL V RAVA+          +FFDKPE LG PIA++R
Sbjct:   19 LNCIDLSTPDVQQSVSLLKQACLDCGFFYVTNHGISQEFMEEVFSQSKKFFELPLSEKMKVLRNEKHRGYTPVLDELLDPDNQIHVGDYKEGYYIGVEVPEDDPEADKPFYGPNVWPADGILPGWRQTMEKFHQQALGVARAVARIIALALDLEADFFDKPEMLGHPIAVMR 190

[Close]

BLAST of EX569786 vs. TrEMBL
Match: B9ILT6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_909566 PE=3 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 6.353e-59
Identity = 110/172 (63.95%), Postives = 136/172 (79.07%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLH-GDFKEGYYIGVEA--NDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN IDLS  ++QQSV+LLKQ+C D GFFY+ NHGISQEFM++VF QS+KFF LPL+EKMKVLRN+  RGYTP+LDE+LDP NQ+H GD+KEGYYIGVE   +D    KPF+GPN+WPA  +LPGWR+TME++ +QAL V RAVA+          +FFDKPE LG PIA++R
Sbjct:   16 LNCIDLSTPDVQQSVSLLKQACLDCGFFYVTNHGISQEFMEEVFSQSKKFFELPLSEKMKVLRNEKHRGYTPVLDELLDPDNQIHVGDYKEGYYIGVEVPEDDPEADKPFYGPNVWPADGILPGWRQTMEKFHQQALGVARAVARIIALALDLEADFFDKPEMLGHPIAVMR 187

[Close]

BLAST of EX569786 vs. TrEMBL
Match: C5XM01_SORBI (Putative uncharacterized protein Sb03g003240 OS=Sorghum bicolor GN=Sb03g003240 PE=3 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 7.766e-57
Identity = 105/171 (61.40%), Postives = 138/171 (80.70%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVE--ANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN I L++ ++Q+SV LLKQ+C DSGFFY+V+HGISQEFMD+VF QS+KFF+LP +EKMK+LR++  RGYTP+LDEILDP+NQ++GD+KEGYYIGVE  A+D   ++PF+GPN WP+ +VLP WRE MEQY R+AL V ++VA+          +FFD+PE LG+PIA LR
Sbjct:   10 LNCISLADPDVQKSVALLKQACLDSGFFYVVDHGISQEFMDEVFSQSKKFFDLPHSEKMKLLRDEKNRGYTPMLDEILDPENQVNGDYKEGYYIGVEVPADDPEANRPFYGPNQWPSKEVLPKWREVMEQYHREALRVAKSVARIIALALDLDVDFFDRPEMLGEPIATLR 180

[Close]

BLAST of EX569786 vs. TrEMBL
Match: C0HHJ5_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 6.575e-56
Identity = 105/171 (61.40%), Postives = 137/171 (80.12%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVE--ANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN I L++ N+Q+SV LLKQ+C DSGFFY+V+HGISQEFMD+VF QS+KFF+LP +EKMK+LR++  RGYTP+LDEILDP+NQ++GD+KEGYYIGVE  A+D   ++PF+GPN WP+ +VLP WRE MEQY R+AL V ++VA+          +FFD+PE LG+ IA LR
Sbjct:   10 LNCISLADPNVQKSVALLKQACLDSGFFYVVDHGISQEFMDEVFAQSKKFFDLPHSEKMKLLRDEKNRGYTPMLDEILDPENQVNGDYKEGYYIGVEVPADDPEANRPFYGPNQWPSKEVLPKWREVMEQYHREALIVAKSVARIIALALDLDVDFFDRPEMLGESIATLR 180

[Close]

BLAST of EX569786 vs. TrEMBL
Match: B4FBV2_MAIZE (Iron ion binding protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 6.575e-56
Identity = 105/171 (61.40%), Postives = 137/171 (80.12%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVE--ANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN I L++ N+Q+SV LLKQ+C DSGFFY+V+HGISQEFMD+VF QS+KFF+LP +EKMK+LR++  RGYTP+LDEILDP+NQ++GD+KEGYYIGVE  A+D   ++PF+GPN WP+ +VLP WRE MEQY R+AL V ++VA+          +FFD+PE LG+ IA LR
Sbjct:   10 LNCISLADPNVQKSVALLKQACLDSGFFYVVDHGISQEFMDEVFAQSKKFFDLPHSEKMKLLRDEKNRGYTPMLDEILDPENQVNGDYKEGYYIGVEVPADDPEANRPFYGPNQWPSKEVLPKWREVMEQYHREALIVAKSVARIIALALDLDVDFFDRPEMLGESIATLR 180

[Close]

BLAST of EX569786 vs. TrEMBL
Match: A2WLJ3_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_00710 PE=3 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 6.154e-54
Identity = 99/171 (57.89%), Postives = 133/171 (77.78%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEA--NDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN I L + + ++SV LLKQ+C DSGFFY+V+HGISQE MD VF QS+KFF+LP+NEKM++LR++  RGYTP+LDEILDP+NQ++GD+KEGYYIG+E   +D   ++PF+GPN WP+ +VLP WR+ MEQY  +AL V +++A+          +FFDKPE LG+PIA LR
Sbjct:   10 LNCISLGDPDTKKSVALLKQACLDSGFFYVVDHGISQELMDDVFAQSKKFFDLPINEKMELLRDEKNRGYTPMLDEILDPENQVNGDYKEGYYIGIEVPEDDPQANRPFYGPNQWPSDEVLPRWRKVMEQYHSEALRVAKSIARIIALALNLEEDFFDKPEMLGEPIATLR 180

[Close]

BLAST of EX569786 vs. SwissProt
Match: Y3291_DICDI (Probable iron/ascorbate oxidoreductase DDB_G0283291 OS=Dictyostelium discoideum GN=DDB_G0283291 PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 3.934e-12
Identity = 49/164 (29.88%), Postives = 81/164 (49.39%), Query Frame = 3

Query:   54 LKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKV---LRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDS----LDHKPFHGPNLWPASDV-----LPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            ++ +C + GFFYI  HGI QE +D++   S+KFF+L  + KMK    L     RGY  +  E+         D+KEG Y+G E ND     +   P HG NL+P  +      + G+++T+  Y  +  ++G ++ +          ++F    T  DP+ + R
Sbjct:   38 IENACKNFGFFYIKGHGIDQELIDRLERLSKKFFSLDQSIKMKYRMELAQKAWRGYFVVGGELTSGLK----DWKEGLYLGTELNDDHPLVIAQTPLHGLNLFPTLEEEIEYDIVGFKDTILTYIDKVTKLGHSLMELIAISLNLSADYFSSRYT-KDPLILYR 196

[Close]

BLAST of EX569786 vs. SwissProt
Match: YQK1_SCHPO (UPF0676 protein C1494.01 OS=Schizosaccharomyces pombe GN=SPCC1494.01 PE=3 SV=2)

HSP 1 Score: 60.077 bits (144), Expect = 6.944e-9
Identity = 35/91 (38.46%), Postives = 50/91 (54.95%), Query Frame = 3

Query:   12 IDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVL-RNDHIR-GYTPLLDEILDPQNQLHGDFKEGY 278
            IDLS  +    V  L  +C + GF  + NHGI  + +D+ F  + KFF++P+ EK K L +   +  GYT    E LD ++Q  GD KE Y
Sbjct:    9 IDLSENDTSIVVKELLDACKNWGFVSLKNHGIPLDEIDRTFKLADKFFDIPVEEKQKYLFKGGRLHSGYTGHFGEKLDMEHQSRGDLKESY 99

[Close]

BLAST of EX569786 vs. SwissProt
Match: SRG1_ARATH (Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.184e-8
Identity = 42/133 (31.58%), Postives = 67/133 (50.38%), Query Frame = 3

Query:   21 SNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKV-LRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGR 416
            S+  +   V  L  +C + GFF +VNHGI   F+DKV  + Q FFNLP+ EK K   R D I G+      ++    +L  D+ + ++  V+  +         P+L+P    LP +R+T+E Y  +   V +
Sbjct:   63 SSTTMDSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAF--VVSEDQKL--DWADLFFHTVQPVE------LRKPHLFPKLP-LP-FRDTLEMYSSEVQSVAK 183

[Close]

BLAST of EX569786 vs. SwissProt
Match: ACCO1_DICMU (1-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium mucoroides GN=aco PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.184e-8
Identity = 44/159 (27.67%), Postives = 75/159 (47.17%), Query Frame = 3

Query:   54 LKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMK---VLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDHKPFHGPNLWPASD-----VLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAIL 506
            + ++C + GFFYI NHG+ QE ++ + +  +KFF+LPL  KMK    L N    G+  +  EI   Q     D+KEG Y   E +   D    H   L+P ++      + G++ T+  Y  +   + + + +          ++F K  T  DP  ++
Sbjct:   30 INKACKEYGFFYIKNHGVDQELIENLQNVIKKFFSLPLEIKMKWKMGLTNREWLGFFKVGQEITYGQ----VDWKEGCYYSSEMDG--DINTIHNVPLYPTAEQEEQYEIQGFKSTIHTYIEKLTHLSQQIIEAISLSLNLPQDYFFKNYTY-DPFILM 181

[Close]

BLAST of EX569786 vs. SwissProt
Match: ACCO1_DICDI (1-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium discoideum GN=aco PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.184e-8
Identity = 44/159 (27.67%), Postives = 75/159 (47.17%), Query Frame = 3

Query:   54 LKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMK---VLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDHKPFHGPNLWPASD-----VLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAIL 506
            + ++C + GFFYI NHG+ QE ++ + +  +KFF+LPL  KMK    L N    G+  +  EI   Q     D+KEG Y   E +   D    H   L+P ++      + G++ T+  Y  +   + + + +          ++F K  T  DP  ++
Sbjct:   30 INKACKEYGFFYIKNHGVDQELIENLQNVIKKFFSLPLEIKMKWKMGLTNREWLGFFKVGQEITYGQ----VDWKEGCYYSSEMDG--DINTIHNVPLYPTAEQEEQYEIQGFKSTIHTYIEKLTHLSQQIIEAISLSLNLPQDYFFKNYTY-DPFILM 181

[Close]

BLAST of EX569786 vs. SwissProt
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=20ox1 PE=2 SV=2)

HSP 1 Score: 55.0694 bits (131), Expect = 2.234e-7
Identity = 25/65 (38.46%), Postives = 41/65 (63.08%), Query Frame = 3

Query:   12 IDLSNV-----NIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLR 191
            IDL N+     +   +  L+ ++C   GFF +VNHGIS+E +    + + +FF++PL+EK +VLR
Sbjct:   64 IDLQNLLSDPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLR 128

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT1G35190.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr1:12890544-12892632 FORWARD LENGTH=329)

HSP 1 Score: 213.001 bits (541), Expect = 7.173e-56
Identity = 101/171 (59.06%), Postives = 127/171 (74.27%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDH--KPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            LN IDL+N ++  SV  LKQ+C D GFFY++NHGIS+EFMD VF+QS+K F LPL EKMKVLRN+  RGYTP+LDE+LDP+NQ++GD KEGYYIG+E      H  KPF+GPN WP +DVLPGWRETME+Y ++AL V  A+A+           +FD+ E LG PIA +R
Sbjct:   12 LNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFEQSKKLFALPLEEKMKVLRNEKHRGYTPVLDELLDPKNQINGDHKEGYYIGIEVPKDDPHWDKPFYGPNPWPDADVLPGWRETMEKYHQEALRVSMAIARLLALALDLDVGYFDRTEMLGKPIATMR 182

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT3G46490.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr3:17115629-17119451 FORWARD LENGTH=330)

HSP 1 Score: 199.519 bits (506), Expect = 8.207e-52
Identity = 93/168 (55.36%), Postives = 120/168 (71.43%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDH--KPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIA 500
            L  IDL N ++ QS  LLKQ+C DSGFFY++NHGIS+E  D+ F+ S+KFF LPL EKMKVLRN+  RGY P  D +LDP+NQ+ GD+KEG+ IG E +    H  KPFH PN+WP  DVLPGWRETME+Y ++AL V +++AK          ++F+ PE LG+PIA
Sbjct:   17 LTCIDLDNSDLHQSAVLLKQACLDSGFFYVINHGISEELKDEAFEHSKKFFALPLEEKMKVLRNEKYRGYAPFHDSLLDPENQVRGDYKEGFTIGFEGSKDGPHWDKPFHSPNIWPNPDVLPGWRETMEKYYQEALRVCKSIAKIMALALDLDVDYFNTPEMLGNPIA 184

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT1G35190.2 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr1:12890544-12892632 FORWARD LENGTH=306)

HSP 1 Score: 191.815 bits (486), Expect = 1.711e-49
Identity = 91/145 (62.76%), Postives = 115/145 (79.31%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDH--KPFHGPNLWPASDVLPGWRETMEQYQRQALE--VGRAVA 425
            LN IDL+N ++  SV  LKQ+C D GFFY++NHGIS+EFMD VF+QS+K F LPL EKMKVLRN+  RGYTP+LDE+LDP+NQ++GD KEGYYIG+E      H  KPF+GPN WP +DVLPGWRETME+Y ++AL   +G+ +A
Sbjct:   12 LNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFEQSKKLFALPLEEKMKVLRNEKHRGYTPVLDELLDPKNQINGDHKEGYYIGIEVPKDDPHWDKPFYGPNPWPDADVLPGWRETMEKYHQEALTEMLGKPIA 156

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT3G46480.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr3:17103173-17105594 FORWARD LENGTH=286)

HSP 1 Score: 129.798 bits (325), Expect = 7.987e-31
Identity = 73/169 (43.20%), Postives = 95/169 (56.21%), Query Frame = 3

Query:    3 LNRIDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            L  IDL+N N QQS   LKQS                          +KFF LPL EKMKVLRN+  RGY+P+LD+ILDP+NQ+ GD+KE ++IG+E                    VLPGWR TME+Y ++AL V +A+A+          N+FDKPE LG+PIA++R
Sbjct:   17 LTCIDLANPNFQQSAVSLKQS--------------------------KKFFALPLEEKMKVLRNEKHRGYSPVLDQILDPENQVDGDYKESFFIGIEV-------------------VLPGWRATMEKYHQEALRVCKAIARLLALALDLDTNYFDKPEMLGNPIAVMR 140

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT4G16770.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr4:9434571-9437168 REVERSE LENGTH=325)

HSP 1 Score: 115.161 bits (287), Expect = 2.036e-26
Identity = 61/166 (36.75%), Postives = 98/166 (59.04%), Query Frame = 3

Query:   12 IDLSNVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            IDLS+     +  L++Q+C D GFFY+ NHG+S+E M+ V  +S+K F+LPL+EKM + R+   RGY+PL DE L+  +   GD KE +  G  +++ +  + +  PN WP  ++LP WR TME Y +  ++VG+ +            N+F++     D  A++R
Sbjct:   20 IDLSSPEKLSTSRLIRQACLDHGFFYLTNHGVSEELMEGVLIESKKLFSLPLDEKMVMARHG-FRGYSPLYDEKLESSSTSIGDSKEMFTFG--SSEGVLGQLY--PNKWPLEELLPLWRPTMECYYKNVMDVGKKLFGLVALALNLEENYFEQVGAFNDQAAVVR 180

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT3G46500.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr3:17120793-17122602 FORWARD LENGTH=251)

HSP 1 Score: 103.99 bits (258), Expect = 4.693e-23
Identity = 48/111 (43.24%), Postives = 74/111 (66.67%), Query Frame = 3

Query:  177 MKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            MKVL+N+  +GY+P+L +I D  NQ+HGD+KE ++IG+E ++     PF   N+WP  DVL GW+ TME+Y ++AL V +A+A+          ++FD PE LG+P+  +R
Sbjct:    1 MKVLKNEKHQGYSPVLSQISD--NQIHGDYKESFFIGIEGSNDT---PFCRANIWPNPDVLSGWQATMEKYHQEALRVCKAIARVLALALNVDGDYFDTPEMLGNPLTFMR 106

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT4G16765.2 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr4:9430467-9431791 REVERSE LENGTH=184)

HSP 1 Score: 76.2554 bits (186), Expect = 1.048e-14
Identity = 43/111 (38.74%), Postives = 55/111 (49.55%), Query Frame = 3

Query:  177 MKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            M +LR D + GYTPL  E LDP     GD KE +Y G          P    N WP+  +LP WR+TME Y +  L VGR +            +FF+K   L DP A++R
Sbjct:    1 MALLRRD-LLGYTPLYAEKLDPSLSSIGDSKESFYFGSLEGVLAQRYP----NQWPSEGILPSWRQTMETYYKNVLSVGRKLLGLIALALDLDEDFFEKVGALNDPTAVVR 106

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT4G16765.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr4:9429974-9431791 REVERSE LENGTH=247)

HSP 1 Score: 76.2554 bits (186), Expect = 1.048e-14
Identity = 43/111 (38.74%), Postives = 55/111 (49.55%), Query Frame = 3

Query:  177 MKVLRNDHIRGYTPLLDEILDPQNQLHGDFKEGYYIGVEANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            M +LR D + GYTPL  E LDP     GD KE +Y G          P    N WP+  +LP WR+TME Y +  L VGR +            +FF+K   L DP A++R
Sbjct:    1 MALLRRD-LLGYTPLYAEKLDPSLSSIGDSKESFYFGSLEGVLAQRYP----NQWPSEGILPSWRQTMETYYKNVLSVGRKLLGLIALALDLDEDFFEKVGALNDPTAVVR 106

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT3G50210.3 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr3:18614338-18616229 REVERSE LENGTH=332)

HSP 1 Score: 71.2478 bits (173), Expect = 3.371e-13
Identity = 45/170 (26.47%), Postives = 83/170 (48.82%), Query Frame = 3

Query:   24 NVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRND--HIRGYTPLLDEILDPQNQLH------GDFKEGYYIGVEANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            +V + + V  L ++C D+GFFY++ HGIS++ ++KV + +++FF LP  EK+K+        RGY  + + +      +H       + K+G Y  +        K   GPN WP +     ++E ME+Y +   ++ R + +            F+  +  GDP  ++R
Sbjct:   28 DVGVAEVVQQLDKACRDAGFFYVIGHGISEDVINKVREITREFFKLPYEEKLKIKMTPAAGYRGYQRIGENVTKGIPDIHEAIDCYREIKQGKYGDI-------GKVMEGPNQWPENP--QEFKELMEEYIKLCTDLSRKILRGISLALAGSPYEFE-GKMAGDPFWVMR 187

[Close]

BLAST of EX569786 vs. TAIR peptide
Match: AT3G50210.1 (| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr3:18614338-18616229 REVERSE LENGTH=332)

HSP 1 Score: 71.2478 bits (173), Expect = 3.371e-13
Identity = 45/170 (26.47%), Postives = 83/170 (48.82%), Query Frame = 3

Query:   24 NVNIQQSVNLLKQSCTDSGFFYIVNHGISQEFMDKVFDQSQKFFNLPLNEKMKVLRND--HIRGYTPLLDEILDPQNQLH------GDFKEGYYIGVEANDSLDHKPFHGPNLWPASDVLPGWRETMEQYQRQALEVGRAVAKXXXXXXXXXXNFFDKPETLGDPIAILR 509
            +V + + V  L ++C D+GFFY++ HGIS++ ++KV + +++FF LP  EK+K+        RGY  + + +      +H       + K+G Y  +        K   GPN WP +     ++E ME+Y +   ++ R + +            F+  +  GDP  ++R
Sbjct:   28 DVGVAEVVQQLDKACRDAGFFYVIGHGISEDVINKVREITREFFKLPYEEKLKIKMTPAAGYRGYQRIGENVTKGIPDIHEAIDCYREIKQGKYGDI-------GKVMEGPNQWPENP--QEFKELMEEYIKLCTDLSRKILRGISLALAGSPYEFE-GKMAGDPFWVMR 187

The following BLAST results are available for this feature:

BLAST of EX569786 vs. TrEMBL
BLAST of EX569786 vs. SwissProt
BLAST of EX569786 vs. TAIR peptide

BLAST of EX569786 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T9F1_SOYBN	2.493e-63	67.84	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6THB6_SOYBN	1.368e-61	67.44	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9RKI5_RICCO	1.672e-59	61.99	Hyoscyamine 6-dioxygenase, putative OS=Ricinus com...	[more]
D7TBH3_VITVI	2.184e-59	63.74	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9ILT7_POPTR	6.353e-59	63.95	2-oxoglutarate-dependent dioxygenase OS=Populus tr...	[more]
B9ILT6_POPTR	6.353e-59	63.95	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C5XM01_SORBI	7.766e-57	61.40	Putative uncharacterized protein Sb03g003240 OS=So...	[more]
C0HHJ5_MAIZE	6.575e-56	61.40	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B4FBV2_MAIZE	6.575e-56	61.40	Iron ion binding protein OS=Zea mays PE=2 SV=1	[more]
A2WLJ3_ORYSI	6.154e-54	57.89	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of EX569786 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 6


Match Name	E-value	Identity	Description
Y3291_DICDI	3.934e-12	29.88	Probable iron/ascorbate oxidoreductase DDB_G028329...	[more]
YQK1_SCHPO	6.944e-9	38.46	UPF0676 protein C1494.01 OS=Schizosaccharomyces po...	[more]
SRG1_ARATH	1.184e-8	31.58	Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 ...	[more]
ACCO1_DICMU	1.184e-8	27.67	1-aminocyclopropane-1-carboxylate oxidase OS=Dicty...	[more]
ACCO1_DICDI	1.184e-8	27.67	1-aminocyclopropane-1-carboxylate oxidase OS=Dicty...	[more]
GAOX1_ARATH	2.234e-7	38.46	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana G...	[more]

BLAST of EX569786 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G35190.1	7.173e-56	59.06	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT3G46490.1	8.207e-52	55.36	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT1G35190.2	1.711e-49	62.76	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT3G46480.1	7.987e-31	43.20	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT4G16770.1	2.036e-26	36.75	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT3G46500.1	4.693e-23	43.24	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT4G16765.2	1.048e-14	38.74	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT4G16765.1	1.048e-14	38.74	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT3G50210.3	3.371e-13	26.47	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]
AT3G50210.1	3.371e-13	26.47	\| Symbols: \| 2-oxoglutarate (2OG) and Fe(II)-depe...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 28..41 score
None	No IPR available	GENE3D	2.60.120.330	no description	coord: 4..159 score: 1.1
None	No IPR available	PANTHER	PTHR10209	IRON/ASCORBATE-DEPENDENT OXIDOREDUCTASE FAMILY MEMBER	coord: 8..169 score: 4
None	No IPR available	PANTHER	PTHR10209:SF6	GIBBERELLIN OXIDASE-RELATED	coord: 8..169 score: 4
None	No IPR available	SEG	seg	seg	coord: 143..152 score
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..169 score: 7.5
None	No IPR available	SEG	seg	seg	coord: 13..23 score
IPR002283	Isopenicillin N synthase	PRINTS	PR00682	IPNSYNTHASE	coord: 18..35 score: 5.2e-10coord: 106..122 score: 5.2e-10coord: 139..155 score: 5.2

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX569786 ID=EX569786; Name=EX569786; organism=Pisum sativum; type=EST; length=509bp
TCCTCAATCGTATTGACCTCTCCAACGTCAATATTCAACAATCCGTTAAT
TTGCTCAAACAGTCTTGCACAGATTCTGGGTTTTTCTATATCGTCAATCA
TGGAATTAGCCAGGAGTTTATGGACAAAGTTTTTGACCAGAGCCAGAAAT
TTTTCAACCTTCCACTCAACGAAAAGATGAAGGTTCTCCGGAACGATCAC
ATTAGGGGATACACTCCTCTTCTCGATGAAATCCTTGATCCTCAGAACCA
ATTACATGGTGATTTTAAAGAAGGCTATTATATTGGAGTTGAAGCTAATG
ATTCACTTGATCACAAACCTTTTCATGGCCCAAACTTATGGCCTGCATCA
GATGTTCTCCCAGGATGGAGGGAAACTATGGAACAATACCAACGCCAGGC
ATTAGAAGTGGGGAGAGCAGTTGCAAAGATAATAGCTCTTGCACTTGATT
TGGATGCTAATTTTTTCGATAAACCAGAAACCCTAGGAGATCCTATAGCG
ATTCTGCGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR002283	Isopenicillin-N_synthase

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