EX570120

EST Overview
NameEX570120
Unique NameEX570120
TypeEST
OrganismPisum sativum (pea)
Sequence length502
Libraries
Library NameType
LIBEST_021973 Pisum sativum shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of EX570120 vs. TrEMBL
Match: B9N7I0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_837406 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.195e-17
Identity = 45/57 (78.95%), Postives = 46/57 (80.70%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSDLG 173
            VKASGILGGPKN  PTVISPKQYKKRFRKAMTTYFLM+PDQW         SQSDLG
Sbjct: 1696 VKASGILGGPKNESPTVISPKQYKKRFRKAMTTYFLMVPDQWSPPSIIPSKSQSDLG 1752
[Close]
BLAST of EX570120 vs. TrEMBL
Match: B9GXZ3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_646054 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.663e-17
Identity = 44/57 (77.19%), Postives = 45/57 (78.95%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSDLG 173
            VKASGILGGPKN  PTVISPKQYKKRFRKAMTTYFLM+PDQW         SQSD G
Sbjct:  597 VKASGILGGPKNASPTVISPKQYKKRFRKAMTTYFLMVPDQWSPPTIILSKSQSDFG 653
[Close]
BLAST of EX570120 vs. TrEMBL
Match: D7TZK4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_2.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016674001 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.012e-16
Identity = 43/56 (76.79%), Postives = 46/56 (82.14%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSDL 170
            VKASGILGGPKN+ PTVISPKQYKKRFRKAMTTYFLM+PDQW         SQS+L
Sbjct: 1586 VKASGILGGPKNSSPTVISPKQYKKRFRKAMTTYFLMVPDQWSPATLIPSKSQSEL 1641
[Close]
BLAST of EX570120 vs. TrEMBL
Match: B9T3Y2_RICCO (Fyve finger-containing phosphoinositide kinase, fyv1, putative OS=Ricinus communis GN=RCOM_0417950 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.012e-16
Identity = 43/56 (76.79%), Postives = 45/56 (80.36%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSDL 170
            VKA+GILGGPKN  PTVISPKQYKKRFRKAMTTYFLM+PDQW         SQSDL
Sbjct: 1771 VKATGILGGPKNASPTVISPKQYKKRFRKAMTTYFLMVPDQWSPPLLIPSKSQSDL 1826
[Close]
BLAST of EX570120 vs. TrEMBL
Match: A5C675_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_043718 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.255e-16
Identity = 42/56 (75.00%), Postives = 46/56 (82.14%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSDL 170
            VKASGILGGP+N+ PTVISPKQYKKRFRKAMTTYFLM+PDQW         SQS+L
Sbjct: 1827 VKASGILGGPRNSAPTVISPKQYKKRFRKAMTTYFLMVPDQWSPATLIPSKSQSEL 1882
[Close]
BLAST of EX570120 vs. TrEMBL
Match: B9RR30_RICCO (Fyve finger-containing phosphoinositide kinase, fyv1, putative OS=Ricinus communis GN=RCOM_0708740 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.461e-15
Identity = 42/56 (75.00%), Postives = 44/56 (78.57%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSDL 170
            VKASGILGG KNT PTVISP+QYKKRFRKAMT YFLM+PDQW         SQSDL
Sbjct: 1754 VKASGILGGSKNTTPTVISPQQYKKRFRKAMTAYFLMVPDQWSPPTIIPSGSQSDL 1809
[Close]
BLAST of EX570120 vs. TrEMBL
Match: D7TC87_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_16.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00015004001 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.252e-15
Identity = 41/56 (73.21%), Postives = 44/56 (78.57%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSDL 170
            VKASGILGGPKNT PTVISP QYKKRFRKAM+ YFLM+PDQW         S+SDL
Sbjct: 1587 VKASGILGGPKNTSPTVISPIQYKKRFRKAMSAYFLMVPDQWSPVIILPSGSKSDL 1642
[Close]
BLAST of EX570120 vs. TrEMBL
Match: Q9LUM0_ARATH (Emb|CAB36798.1 OS=Arabidopsis thaliana GN=At3g14270 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.253e-15
Identity = 39/55 (70.91%), Postives = 44/55 (80.00%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSD 167
            VK +GILGGPKN  PTVISPKQYK+RFRKAMTTYFLM+PDQW        +S+SD
Sbjct: 1724 VKFTGILGGPKNEAPTVISPKQYKRRFRKAMTTYFLMVPDQWSPPNVVANNSKSD 1778
[Close]
BLAST of EX570120 vs. TrEMBL
Match: D7L328_ARALY (Phosphatidylinositol-4-phosphate 5-kinase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_478844 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.253e-15
Identity = 39/55 (70.91%), Postives = 44/55 (80.00%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSD 167
            VK +GILGGPKN  PTVISPKQYK+RFRKAMTTYFLM+PDQW        +S+SD
Sbjct: 1722 VKFTGILGGPKNEAPTVISPKQYKRRFRKAMTTYFLMVPDQWSPPNVVANNSRSD 1776
[Close]
BLAST of EX570120 vs. TrEMBL
Match: D5ABH6_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.253e-15
Identity = 38/42 (90.48%), Postives = 40/42 (95.24%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQW 128
            VKASGILGGPKN  PTVISPKQYKKRFRKAM+TYFLM+PDQW
Sbjct:  245 VKASGILGGPKNISPTVISPKQYKKRFRKAMSTYFLMVPDQW 286
[Close]
BLAST of EX570120 vs. SwissProt
Match: FYV1_DROME (Putative 1-phosphatidylinositol-3-phosphate 5-kinase OS=Drosophila melanogaster GN=fab1 PE=1 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 6.308e-7
Identity = 24/42 (57.14%), Postives = 32/42 (76.19%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQW 128
            +K SGILGG K   PTV++P++YK+RF  AM  YFL +PD+W
Sbjct: 1763 IKGSGILGG-KGKDPTVVNPERYKQRFIDAMDRYFLTVPDRW 1803
[Close]
BLAST of EX570120 vs. SwissProt
Match: FAB1_YEAST (1-phosphatidylinositol-3-phosphate 5-kinase FAB1 OS=Saccharomyces cerevisiae GN=FAB1 PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 8.239e-7
Identity = 24/43 (55.81%), Postives = 31/43 (72.09%), Query Frame = 3 
Query:    3 VKASGILGGPKNTP-PTVISPKQYKKRFRKAMTTYFLMLPDQW 128
            VK  G++GG      PTV++P+QYKKRFR+AM  Y LM+PD W
Sbjct: 2231 VKEKGLVGGASVIKQPTVVTPRQYKKRFREAMERYILMVPDPW 2273
[Close]
BLAST of EX570120 vs. TAIR peptide
Match: AT3G14270.1 (| Symbols: FAB1B | phosphatidylinositol-4-phosphate 5-kinase family protein | chr3:4754624-4761185 FORWARD LENGTH=1791)

HSP 1 Score: 83.5741 bits (205), Expect = 6.305e-17
Identity = 39/55 (70.91%), Postives = 44/55 (80.00%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQSD 167
            VK +GILGGPKN  PTVISPKQYK+RFRKAMTTYFLM+PDQW        +S+SD
Sbjct: 1724 VKFTGILGGPKNEAPTVISPKQYKRRFRKAMTTYFLMVPDQWSPPNVVANNSKSD 1778
[Close]
BLAST of EX570120 vs. TAIR peptide
Match: AT4G33240.3 (| Symbols: FAB1A | 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases | chr4:16029504-16037278 REVERSE LENGTH=1753)

HSP 1 Score: 80.4925 bits (197), Expect = 5.337e-16
Identity = 37/54 (68.52%), Postives = 42/54 (77.78%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQS 164
            VK SG+LGGPKN+ PTVISP+QYKKRFRKAMT YFLM+PDQW        +S S
Sbjct: 1681 VKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTAYFLMVPDQWSPAAVVPSNSSS 1734
[Close]
BLAST of EX570120 vs. TAIR peptide
Match: AT4G33240.2 (| Symbols: FAB1A | 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases | chr4:16029504-16037278 REVERSE LENGTH=1756)

HSP 1 Score: 80.4925 bits (197), Expect = 5.337e-16
Identity = 37/54 (68.52%), Postives = 42/54 (77.78%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQS 164
            VK SG+LGGPKN+ PTVISP+QYKKRFRKAMT YFLM+PDQW        +S S
Sbjct: 1684 VKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTAYFLMVPDQWSPAAVVPSNSSS 1737
[Close]
BLAST of EX570120 vs. TAIR peptide
Match: AT4G33240.1 (| Symbols: FAB1A | 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases | chr4:16029504-16037278 REVERSE LENGTH=1757)

HSP 1 Score: 80.4925 bits (197), Expect = 5.337e-16
Identity = 37/54 (68.52%), Postives = 42/54 (77.78%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQWXXXXXXXXHSQS 164
            VK SG+LGGPKN+ PTVISP+QYKKRFRKAMT YFLM+PDQW        +S S
Sbjct: 1685 VKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTAYFLMVPDQWSPAAVVPSNSSS 1738
[Close]
BLAST of EX570120 vs. TAIR peptide
Match: AT1G71010.1 (| Symbols: FAB1C | FORMS APLOID AND BINUCLEATE CELLS 1C | chr1:26782839-26788712 FORWARD LENGTH=1648)

HSP 1 Score: 77.411 bits (189), Expect = 4.518e-15
Identity = 33/42 (78.57%), Postives = 38/42 (90.48%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQW 128
            VKASGILGGPKN  PT++SPKQYK+RFRKAMTTYFL +P+ W
Sbjct: 1605 VKASGILGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEPW 1646
[Close]
BLAST of EX570120 vs. TAIR peptide
Match: AT1G34260.1 (| Symbols: FAB1D | FORMS APLOID AND BINUCLEATE CELLS 1A | chr1:12485967-12491799 FORWARD LENGTH=1456)

HSP 1 Score: 53.1434 bits (126), Expect = 9.125e-8
Identity = 26/42 (61.90%), Postives = 31/42 (73.81%), Query Frame = 3 
Query:    3 VKASGILGGPKNTPPTVISPKQYKKRFRKAMTTYFLMLPDQW 128
            VK+S ++  PKN  PTVISP  YK RFRK M T+FL +PDQW
Sbjct: 1411 VKSSLVV--PKNVQPTVISPIDYKTRFRKFMKTHFLCVPDQW 1450
The following BLAST results are available for this feature:
BLAST of EX570120 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B9N7I0_POPTR1.195e-1778.95Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9GXZ3_POPTR2.663e-1777.19Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7TZK4_VITVI1.012e-1676.79Whole genome shotgun sequence of line PN40024, sca... [more]
B9T3Y2_RICCO1.012e-1676.79Fyve finger-containing phosphoinositide kinase, fy... [more]
A5C675_VITVI2.255e-1675.00Putative uncharacterized protein OS=Vitis vinifera... [more]
B9RR30_RICCO1.461e-1575.00Fyve finger-containing phosphoinositide kinase, fy... [more]
D7TC87_VITVI4.252e-1573.21Whole genome shotgun sequence of line PN40024, sca... [more]
Q9LUM0_ARATH7.253e-1570.91Emb|CAB36798.1 OS=Arabidopsis thaliana GN=At3g1427... [more]
D7L328_ARALY7.253e-1570.91Phosphatidylinositol-4-phosphate 5-kinase family p... [more]
D5ABH6_PICSI7.253e-1590.48Putative uncharacterized protein OS=Picea sitchens... [more]
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BLAST of EX570120 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2
Match NameE-valueIdentityDescription
FYV1_DROME6.308e-757.14Putative 1-phosphatidylinositol-3-phosphate 5-kina... [more]
FAB1_YEAST8.239e-755.811-phosphatidylinositol-3-phosphate 5-kinase FAB1 O... [more]
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BLAST of EX570120 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6
Match NameE-valueIdentityDescription
AT3G14270.16.305e-1770.91| Symbols: FAB1B | phosphatidylinositol-4-phosphat... [more]
AT4G33240.35.337e-1668.52| Symbols: FAB1A | 1-phosphatidylinositol-4-phosph... [more]
AT4G33240.25.337e-1668.52| Symbols: FAB1A | 1-phosphatidylinositol-4-phosph... [more]
AT4G33240.15.337e-1668.52| Symbols: FAB1A | 1-phosphatidylinositol-4-phosph... [more]
AT1G71010.14.518e-1578.57| Symbols: FAB1C | FORMS APLOID AND BINUCLEATE CEL... [more]
AT1G34260.19.125e-861.90| Symbols: FAB1D | FORMS APLOID AND BINUCLEATE CEL... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePROFILEPS51455PIPKcoord: 1..35
score: 9
NoneNo IPR availableSEGsegsegcoord: 43..50
score: NAcoord: 58..68
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>EX570120 ID=EX570120; Name=EX570120; organism=Pisum sativum; type=EST; length=502bp
GGGTGAAAGCTTCAGGAATCCTTGGAGGACCAAAGAACACACCACCAACA
GTTATATCACCAAAGCAGTACAAGAAAAGGTTCAGGAAAGCAATGACCAC
TTACTTTCTCATGCTTCCAGATCAGTGGTCCCCACCATCTATTATTCCTA
GTCATTCTCAGTCTGATTTAGGTGAAGAAAAAGAAAACAAAGAAAAAGAA
AACAATACACAGTCTAGGCCCTTAGCTGAATGATTCTTTCTTTCATGTGT
TTGTGTAAATTTTGCTTCCATTTTGGGGAATCATCAACTCCATCCATATT
TTTTATTTGGTTTTTGTTTTTGGCCTCTTAAATTCTATAGGAATTCTTTT
GGTTCAATTGTAATAGATACACACACAAAGCTGTTAGAAGCCACATGGTG
TTTGTCTGGAGTAAATGTATTTAATGACCTCCAGATGAAAATCTATGTAC
AAAAAACATATAGTCTGTTTCCAGCATCATATATACATGTATAATTTTTC
AG
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0016307obsolete phosphatidylinositol phosphate kinase activity
Vocabulary: Biological Process
TermDefinition
GO:0046488phosphatidylinositol metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR002498PInositol-4-P-4/5-kinase_core