EX570234

EST Overview


Name	EX570234
Unique Name	EX570234
Type	EST
Organism	Pisum sativum (pea)
Sequence length	419

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of EX570234 vs. TrEMBL
Match: B9HA10_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_716975 PE=4 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 2.064e-59
Identity = 71/81 (87.65%), Postives = 76/81 (93.83%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG TFNHRYVK+NPHEVENATWMLTVFNC+GR FCLHFEAFQLG APV+MAFLRFMGDDNE KKFSYSLEVG +GRKL+WQ
Sbjct:  177 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLVWQ 257

HSP 2 Score: 93.2041 bits (230), Expect = 2.064e-59
Identity = 42/49 (85.71%), Postives = 47/49 (95.92%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED 392
            IPRS+RDSH KVRDSQDGLIIQRN ALYFSGGD+QELKLR+TGR+WKE+
Sbjct:  259 IPRSIRDSHRKVRDSQDGLIIQRNLALYFSGGDRQELKLRVTGRVWKEE 307

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BLAST of EX570234 vs. TrEMBL
Match: C6TM08_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 2.691e-59
Identity = 73/81 (90.12%), Postives = 77/81 (95.06%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG TFNHRYVK NPHEVENATWMLTVFN +GRHFCLHFEAFQLG+APV+MAFLRFMGDDNE KKFSYSLEVGA+GRKLIWQ
Sbjct:  179 DGCTFNHRYVKANPHEVENATWMLTVFNSFGRHFCLHFEAFQLGSAPVYMAFLRFMGDDNEAKKFSYSLEVGANGRKLIWQ 259

HSP 2 Score: 91.2781 bits (225), Expect = 2.691e-59
Identity = 42/49 (85.71%), Postives = 47/49 (95.92%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED 392
            IPRS+RDSH KVRDSQDGLIIQRN ALYFSGG++QELKLRITGRIW+E+
Sbjct:  261 IPRSIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWREE 309

[Close]

BLAST of EX570234 vs. TrEMBL
Match: B9IIA7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_667497 PE=4 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 5.965e-59
Identity = 71/81 (87.65%), Postives = 76/81 (93.83%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG TFNHRYVK+NPHEVENATWMLTVFNC+GR FCLHFEAFQLG APV+MAFLRFMGDDNE KKFSYSLEVG +GRKL+WQ
Sbjct:  174 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLVWQ 254

HSP 2 Score: 91.6633 bits (226), Expect = 5.965e-59
Identity = 41/49 (83.67%), Postives = 47/49 (95.92%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED 392
            IPRS+RDSH KVRDSQDGLIIQRN ALYFSGGD++ELKLR+TGR+WKE+
Sbjct:  256 IPRSIRDSHRKVRDSQDGLIIQRNLALYFSGGDRKELKLRVTGRVWKEE 304

[Close]

BLAST of EX570234 vs. TrEMBL
Match: B9T806_RICCO (Ubiquitin ligase SINAT2, putative OS=Ricinus communis GN=RCOM_0446280 PE=4 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 5.965e-59
Identity = 71/81 (87.65%), Postives = 76/81 (93.83%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG TFNHRYVK+NPHEVENATWMLTVFNC+GR FCLHFEAFQ+G APV+MAFLRFMGDDNE KKFSYSLEVG +GRKLIWQ
Sbjct:  174 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQIGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQ 254

HSP 2 Score: 92.0485 bits (227), Expect = 5.965e-59
Identity = 43/49 (87.76%), Postives = 47/49 (95.92%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED 392
            IPRS+RDSH KVRDSQDGLIIQR+ ALYFSGGD+QELKLRITGRIWKE+
Sbjct:  256 IPRSIRDSHRKVRDSQDGLIIQRSLALYFSGGDRQELKLRITGRIWKEE 304

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BLAST of EX570234 vs. TrEMBL
Match: Q9XGC3_VITVI (SINA2p OS=Vitis vinifera GN=VIT_00033473001 PE=2 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 4.979e-58
Identity = 70/81 (86.42%), Postives = 75/81 (92.59%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG TFNHRYVK+NP EVENATWMLTVFNC+G+ FCLHFEAFQLGTAPV+MAFLRFMGDDNE KKFSYSLEVG + RKLIWQ
Sbjct:  177 DGCTFNHRYVKSNPQEVENATWMLTVFNCFGKQFCLHFEAFQLGTAPVYMAFLRFMGDDNEAKKFSYSLEVGGNSRKLIWQ 257

HSP 2 Score: 92.4337 bits (228), Expect = 4.979e-58
Identity = 42/48 (87.50%), Postives = 46/48 (95.83%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE 389
            +PRS+RDSH KVRDSQDGLIIQRN ALYFSGGD+QELKLR+TGRIWKE
Sbjct:  259 VPRSIRDSHRKVRDSQDGLIIQRNLALYFSGGDRQELKLRVTGRIWKE 306

[Close]

BLAST of EX570234 vs. TrEMBL
Match: A9SSC3_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_215197 PE=4 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.106e-57
Identity = 70/81 (86.42%), Postives = 77/81 (95.06%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            +GFTFNHRYVK+NP EVENATWMLTVFNC+G+HFCLHFEAFQLG APV+MAFLRFMGDDNE K FSYSLEVGA+GRKL+WQ
Sbjct:  112 NGFTFNHRYVKSNPQEVENATWMLTVFNCFGQHFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKNFSYSLEVGANGRKLVWQ 192

HSP 2 Score: 88.1965 bits (217), Expect = 1.106e-57
Identity = 39/48 (81.25%), Postives = 45/48 (93.75%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE 389
            +PRS+RDSH KVRDS DGLIIQRN AL+FSGGD++ELKLR+TGRIWKE
Sbjct:  194 VPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKE 241

[Close]

BLAST of EX570234 vs. TrEMBL
Match: A9RY27_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_206770 PE=4 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 7.085e-57
Identity = 70/81 (86.42%), Postives = 76/81 (93.83%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            +G TFNHRYVK+NP EVENATWMLTVFNC+G+HFCLHFEAFQLG APV+MAFLRFMGDDNE K FSYSLEVGA+GRKLIWQ
Sbjct:  112 NGCTFNHRYVKSNPQEVENATWMLTVFNCFGQHFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKNFSYSLEVGANGRKLIWQ 192

HSP 2 Score: 88.1965 bits (217), Expect = 7.085e-57
Identity = 39/48 (81.25%), Postives = 45/48 (93.75%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE 389
            +PRS+RDSH KVRDS DGLIIQRN AL+FSGGD++ELKLR+TGRIWKE
Sbjct:  194 VPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKE 241

[Close]

BLAST of EX570234 vs. TrEMBL
Match: A9NV72_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 2.222e-55
Identity = 67/81 (82.72%), Postives = 72/81 (88.89%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            +G TFNHRYVK NP EVENATWMLTVFNC+G+ FCLHFEAFQLG APV+MAFLRFMGDDNE K F YSLEVGA+GRKL WQ
Sbjct:  175 NGCTFNHRYVKPNPQEVENATWMLTVFNCFGQQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKNFGYSLEVGANGRKLCWQ 255

HSP 2 Score: 90.5077 bits (223), Expect = 2.222e-55
Identity = 41/48 (85.42%), Postives = 45/48 (93.75%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE 389
            IPRS+RDSH KVRDS DGLIIQRN AL+FSGGD+QELKLR+TGRIWKE
Sbjct:  257 IPRSIRDSHKKVRDSHDGLIIQRNMALFFSGGDRQELKLRVTGRIWKE 304

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BLAST of EX570234 vs. TrEMBL
Match: A9NX30_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 8.363e-55
Identity = 67/80 (83.75%), Postives = 75/80 (93.75%), Query Frame = 1

Query:    4 GFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            G TFNHRYVK+NP EVENATWMLTVF+C+G++FCLHFEAFQLG APV+MAFLRFMGDDNE K FSYSLEVGA+GRK+IWQ
Sbjct:  194 GCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKNFSYSLEVGANGRKMIWQ 273

HSP 2 Score: 86.6557 bits (213), Expect = 8.363e-55
Identity = 38/48 (79.17%), Postives = 45/48 (93.75%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE 389
            +PRS+RDSH KVR+S DGLIIQRN AL+FSGGD++ELKLR+TGRIWKE
Sbjct:  275 VPRSIRDSHRKVRESHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKE 322

[Close]

BLAST of EX570234 vs. TrEMBL
Match: A9PB73_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_711983 PE=2 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 1.854e-54
Identity = 65/81 (80.25%), Postives = 75/81 (92.59%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            +G TFNHRYVK+NPHEVENATWMLTVF+C+G++FCLHFEAFQLG APV++AFLRFMGDDNE K +SYSLEVG  GRK+IWQ
Sbjct:  175 NGSTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLRFMGDDNEAKNYSYSLEVGGGGRKMIWQ 255

HSP 2 Score: 87.0409 bits (214), Expect = 1.854e-54
Identity = 39/48 (81.25%), Postives = 45/48 (93.75%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE 389
            +PRS+RDSH KVRDS DGLIIQRN AL+FSGGD++ELKLR+TGRIWKE
Sbjct:  257 VPRSIRDSHRKVRDSFDGLIIQRNMALFFSGGDRKELKLRVTGRIWKE 304

[Close]

BLAST of EX570234 vs. SwissProt
Match: SINA3_ARATH (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.409e-53
Identity = 61/80 (76.25%), Postives = 72/80 (90.00%), Query Frame = 1

Query:    4 GFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            G TFNHRYVK+NP EVENATWMLTVF+C+G++FCLHFEAFQLG APV+MAFLRFMGD+ E + ++YSLEVG  GRKLIW+
Sbjct:  182 GCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWE 261

HSP 2 Score: 88.9669 bits (219), Expect = 1.409e-53
Identity = 41/52 (78.85%), Postives = 46/52 (88.46%), Query Frame = 3

Query:  249 PRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED*EKG 404
            PRSVRDSH KVRDS DGLIIQRN AL+FSGGD++ELKLR+TGRIWKE  + G
Sbjct:  264 PRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQQQSG 315

[Close]

BLAST of EX570234 vs. SwissProt
Match: SINA5_ARATH (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 136.346 bits (342), Expect = 1.997e-52
Identity = 58/80 (72.50%), Postives = 70/80 (87.50%), Query Frame = 1

Query:    4 GFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            G TFNHRYVK+NP EVENATWMLTVF C+G++FCLHFEAFQLG APV+MAFLRFMGD+++ + ++YSLEVG  GRK  W+
Sbjct:  165 GCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWE 244

HSP 2 Score: 89.3521 bits (220), Expect = 1.997e-52
Identity = 45/58 (77.59%), Postives = 50/58 (86.21%), Query Frame = 3

Query:  249 PRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE--D*EKGVNIT 416
            PRSVRDSH KVRDS DGLIIQRN AL+FSGGDK+ELKLR+TGRIWKE  + + GV IT
Sbjct:  247 PRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQQNPDSGVCIT 304

[Close]

BLAST of EX570234 vs. SwissProt
Match: SINA4_ARATH (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 5.762e-52
Identity = 59/80 (73.75%), Postives = 70/80 (87.50%), Query Frame = 1

Query:    4 GFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            G TFNHRYVK+NP EVENATWMLTVF+C+G++FCLHFEAFQLG  PV+MAFLRFMGD+ + + +SYSLEVG  GRKL W+
Sbjct:  183 GSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWE 262

HSP 2 Score: 85.8853 bits (211), Expect = 5.762e-52
Identity = 38/47 (80.85%), Postives = 44/47 (93.62%), Query Frame = 3

Query:  249 PRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE 389
            PRS+RDSH KVRDS DGLIIQRN AL+FSGGD++ELKLR+TG+IWKE
Sbjct:  265 PRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIWKE 311

[Close]

BLAST of EX570234 vs. SwissProt
Match: SINA2_ARATH (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=2 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 1.457e-38
Identity = 71/81 (87.65%), Postives = 75/81 (92.59%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG TFNHRYVK+NPHEVENATWMLTVFNC+GR FCLHFEAFQLG APV+MAFLRFMGD+NE KKFSYSLEVGA GRKL WQ
Sbjct:  178 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQ 258

HSP 2 Score: 92.4337 bits (228), Expect = 7.516e-19
Identity = 51/107 (47.66%), Postives = 60/107 (56.07%), Query Frame = 3

Query:   72 NCFQLLRATFLLAL*GFPAWHSSGFHGLLTVYGRRQRGEEIQLQLRSRCRWAQTYMASIPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED 392
            NCF      F L    F    +  +   L   G     ++    L       +     IPRS+RDSH KVRDSQDGLII RN ALYFSGGD+QELKLR+TGRIWKE+
Sbjct:  205 NCFG---RQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

[Close]

BLAST of EX570234 vs. SwissProt
Match: SINA1_ARATH (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE=2 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 7.229e-38
Identity = 70/81 (86.42%), Postives = 74/81 (91.36%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG TFNHRYVK+NPHEVENATWMLTVFNC+GR FCLHFEAFQLG APV+MAFLRFMGD+NE KKFSYSLEVGA  RKL WQ
Sbjct:  175 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQ 255

HSP 2 Score: 89.7373 bits (221), Expect = 4.871e-18
Identity = 50/107 (46.73%), Postives = 59/107 (55.14%), Query Frame = 3

Query:   72 NCFQLLRATFLLAL*GFPAWHSSGFHGLLTVYGRRQRGEEIQLQLRSRCRWAQTYMASIPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED 392
            NCF      F L    F    +  +   L   G     ++    L       +     IPRS+RDSH KVRDSQDGLII RN ALYFSG DK+ELKLR+TGRIWKE+
Sbjct:  202 NCFG---RQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWKEE 305

[Close]

BLAST of EX570234 vs. TAIR peptide
Match: AT3G61790.1 (| Symbols: | Protein with RING/U-box and TRAF-like domains | chr3:22871974-22873543 REVERSE LENGTH=326)

HSP 1 Score: 140.584 bits (353), Expect = 1.719e-54
Identity = 61/80 (76.25%), Postives = 72/80 (90.00%), Query Frame = 1

Query:    4 GFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            G TFNHRYVK+NP EVENATWMLTVF+C+G++FCLHFEAFQLG APV+MAFLRFMGD+ E + ++YSLEVG  GRKLIW+
Sbjct:  182 GCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWE 261

HSP 2 Score: 88.9669 bits (219), Expect = 1.719e-54
Identity = 41/52 (78.85%), Postives = 46/52 (88.46%), Query Frame = 3

Query:  249 PRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED*EKG 404
            PRSVRDSH KVRDS DGLIIQRN AL+FSGGD++ELKLR+TGRIWKE  + G
Sbjct:  264 PRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQQQSG 315

[Close]

BLAST of EX570234 vs. TAIR peptide
Match: AT5G53360.1 (| Symbols: | TRAF-like superfamily protein | chr5:21648485-21649269 FORWARD LENGTH=233)

HSP 1 Score: 136.346 bits (342), Expect = 2.444e-53
Identity = 58/80 (72.50%), Postives = 70/80 (87.50%), Query Frame = 1

Query:    4 GFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            G TFNHRYVK+NP EVENATWMLTVF C+G++FCLHFEAFQLG APV+MAFLRFMGD+++ + ++YSLEVG  GRK  W+
Sbjct:   89 GCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWE 168

HSP 2 Score: 89.3521 bits (220), Expect = 2.444e-53
Identity = 45/58 (77.59%), Postives = 50/58 (86.21%), Query Frame = 3

Query:  249 PRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE--D*EKGVNIT 416
            PRSVRDSH KVRDS DGLIIQRN AL+FSGGDK+ELKLR+TGRIWKE  + + GV IT
Sbjct:  171 PRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQQNPDSGVCIT 228

[Close]

BLAST of EX570234 vs. TAIR peptide
Match: AT4G27880.1 (| Symbols: | Protein with RING/U-box and TRAF-like domains | chr4:13883623-13884927 FORWARD LENGTH=327)

HSP 1 Score: 138.272 bits (347), Expect = 7.030e-53
Identity = 59/80 (73.75%), Postives = 70/80 (87.50%), Query Frame = 1

Query:    4 GFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            G TFNHRYVK+NP EVENATWMLTVF+C+G++FCLHFEAFQLG  PV+MAFLRFMGD+ + + +SYSLEVG  GRKL W+
Sbjct:  183 GSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWE 262

HSP 2 Score: 85.8853 bits (211), Expect = 7.030e-53
Identity = 38/47 (80.85%), Postives = 44/47 (93.62%), Query Frame = 3

Query:  249 PRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKE 389
            PRS+RDSH KVRDS DGLIIQRN AL+FSGGD++ELKLR+TG+IWKE
Sbjct:  265 PRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIWKE 311

[Close]

BLAST of EX570234 vs. TAIR peptide
Match: AT3G58040.1 (| Symbols: SINAT2 | seven in absentia of Arabidopsis 2 | chr3:21489612-21491085 FORWARD LENGTH=308)

HSP 1 Score: 157.918 bits (398), Expect = 1.782e-39
Identity = 71/81 (87.65%), Postives = 75/81 (92.59%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG TFNHRYVK+NPHEVENATWMLTVFNC+GR FCLHFEAFQLG APV+MAFLRFMGD+NE KKFSYSLEVGA GRKL WQ
Sbjct:  178 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQ 258

HSP 2 Score: 92.4337 bits (228), Expect = 9.197e-20
Identity = 51/107 (47.66%), Postives = 60/107 (56.07%), Query Frame = 3

Query:   72 NCFQLLRATFLLAL*GFPAWHSSGFHGLLTVYGRRQRGEEIQLQLRSRCRWAQTYMASIPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED 392
            NCF      F L    F    +  +   L   G     ++    L       +     IPRS+RDSH KVRDSQDGLII RN ALYFSGGD+QELKLR+TGRIWKE+
Sbjct:  205 NCFG---RQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

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BLAST of EX570234 vs. TAIR peptide
Match: AT2G41980.1 (| Symbols: | Protein with RING/U-box and TRAF-like domains | chr2:17523494-17524692 REVERSE LENGTH=305)

HSP 1 Score: 155.606 bits (392), Expect = 8.846e-39
Identity = 70/81 (86.42%), Postives = 74/81 (91.36%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG TFNHRYVK+NPHEVENATWMLTVFNC+GR FCLHFEAFQLG APV+MAFLRFMGD+NE KKFSYSLEVGA  RKL WQ
Sbjct:  175 DGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQ 255

HSP 2 Score: 89.7373 bits (221), Expect = 5.961e-19
Identity = 50/107 (46.73%), Postives = 59/107 (55.14%), Query Frame = 3

Query:   72 NCFQLLRATFLLAL*GFPAWHSSGFHGLLTVYGRRQRGEEIQLQLRSRCRWAQTYMASIPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDKQELKLRITGRIWKED 392
            NCF      F L    F    +  +   L   G     ++    L       +     IPRS+RDSH KVRDSQDGLII RN ALYFSG DK+ELKLR+TGRIWKE+
Sbjct:  202 NCFG---RQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWKEE 305

[Close]

BLAST of EX570234 vs. TAIR peptide
Match: AT3G13672.2 (| Symbols: | TRAF-like superfamily protein | chr3:4473541-4474343 REVERSE LENGTH=220)

HSP 1 Score: 109.768 bits (273), Expect = 4.816e-38
Identity = 47/81 (58.02%), Postives = 62/81 (76.54%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG +F+HRYV  +P  + +ATWMLT+ +C GR FCL+FEAF L   P++MAF++FMGD+ E   FSYSL+VG +GRKL WQ
Sbjct:   83 DGRSFSHRYVHHDPKHLHHATWMLTLLDCCGRKFCLYFEAFHLRKTPMYMAFMQFMGDEEEAMSFSYSLQVGGNGRKLTWQ 163

HSP 2 Score: 64.6994 bits (156), Expect = 4.816e-38
Identity = 30/51 (58.82%), Postives = 40/51 (78.43%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDK---QELKLRITGRIWKE 389
            +PRS+RDSH  VRDSQDGLII R  AL+FS  +    +ELKL+++GR+W+E
Sbjct:  165 VPRSIRDSHKTVRDSQDGLIITRKLALFFSTDNNTTDKELKLKVSGRVWRE 215

[Close]

BLAST of EX570234 vs. TAIR peptide
Match: AT3G13672.1 (| Symbols: | TRAF-like superfamily protein | chr3:4473541-4474343 REVERSE LENGTH=216)

HSP 1 Score: 109.768 bits (273), Expect = 4.817e-38
Identity = 47/81 (58.02%), Postives = 62/81 (76.54%), Query Frame = 1

Query:    1 DGFTFNHRYVKTNPHEVENATWMLTVFNCYGRHFCLHFEAFQLGTAPVFMAFLRFMGDDNEEKKFSYSLEVGADGRKLIWQ 243
            DG +F+HRYV  +P  + +ATWMLT+ +C GR FCL+FEAF L   P++MAF++FMGD+ E   FSYSL+VG +GRKL WQ
Sbjct:   79 DGRSFSHRYVHHDPKHLHHATWMLTLLDCCGRKFCLYFEAFHLRKTPMYMAFMQFMGDEEEAMSFSYSLQVGGNGRKLTWQ 159

HSP 2 Score: 64.6994 bits (156), Expect = 4.817e-38
Identity = 30/51 (58.82%), Postives = 40/51 (78.43%), Query Frame = 3

Query:  246 IPRSVRDSHSKVRDSQDGLIIQRNQALYFSGGDK---QELKLRITGRIWKE 389
            +PRS+RDSH  VRDSQDGLII R  AL+FS  +    +ELKL+++GR+W+E
Sbjct:  161 VPRSIRDSHKTVRDSQDGLIITRKLALFFSTDNNTTDKELKLKVSGRVWRE 211

The following BLAST results are available for this feature:

BLAST of EX570234 vs. TrEMBL
BLAST of EX570234 vs. SwissProt
BLAST of EX570234 vs. TAIR peptide

BLAST of EX570234 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HA10_POPTR	2.064e-59	87.65	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C6TM08_SOYBN	2.691e-59	90.12	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9IIA7_POPTR	5.965e-59	87.65	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9T806_RICCO	5.965e-59	87.65	Ubiquitin ligase SINAT2, putative OS=Ricinus commu...	[more]
Q9XGC3_VITVI	4.979e-58	86.42	SINA2p OS=Vitis vinifera GN=VIT_00033473001 PE=2 S...	[more]
A9SSC3_PHYPA	1.106e-57	86.42	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
A9RY27_PHYPA	7.085e-57	86.42	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
A9NV72_PICSI	2.222e-55	82.72	Putative uncharacterized protein OS=Picea sitchens...	[more]
A9NX30_PICSI	8.363e-55	83.75	Putative uncharacterized protein OS=Picea sitchens...	[more]
A9PB73_POPTR	1.854e-54	80.25	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of EX570234 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 5


Match Name	E-value	Identity	Description
SINA3_ARATH	1.409e-53	76.25	E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis ...	[more]
SINA5_ARATH	1.997e-52	72.50	E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis ...	[more]
SINA4_ARATH	5.762e-52	73.75	E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis ...	[more]
SINA2_ARATH	1.457e-38	87.65	E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis ...	[more]
SINA1_ARATH	7.229e-38	86.42	Putative E3 ubiquitin-protein ligase SINAT1 OS=Ara...	[more]

BLAST of EX570234 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT3G61790.1	1.719e-54	76.25	\| Symbols: \| Protein with RING/U-box and TRAF-lik...	[more]
AT5G53360.1	2.444e-53	72.50	\| Symbols: \| TRAF-like superfamily protein \| chr5...	[more]
AT4G27880.1	7.030e-53	73.75	\| Symbols: \| Protein with RING/U-box and TRAF-lik...	[more]
AT3G58040.1	1.782e-39	87.65	\| Symbols: SINAT2 \| seven in absentia of Arabidops...	[more]
AT2G41980.1	8.846e-39	86.42	\| Symbols: \| Protein with RING/U-box and TRAF-lik...	[more]
AT3G13672.2	4.816e-38	58.02	\| Symbols: \| TRAF-like superfamily protein \| chr3...	[more]
AT3G13672.1	4.817e-38	58.02	\| Symbols: \| TRAF-like superfamily protein \| chr3...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004162	Seven-in-absentia protein, sina	PANTHER	PTHR10315	SEVEN IN ABSENTIA HOMOLOG	coord: 4..81 score: 9
IPR004162	Seven-in-absentia protein, sina	PANTHER	PTHR10315	SEVEN IN ABSENTIA HOMOLOG	coord: 44..92 score: 1.7
IPR008974	TRAF-like	SUPERFAMILY	49599	TRAF domain-like	coord: 3..84 score: 1.5
IPR008974	TRAF-like	SUPERFAMILY	49599	TRAF domain-like	coord: 13..89 score: 2.1
IPR013322	TRAF-type	GENE3D	2.60.210.10	no description	coord: 2..83 score: 2.7
IPR013322	TRAF-type	GENE3D	2.60.210.10	no description	coord: 39..86 score: 1.6
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 3..83 score: 2
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 15..85 score: 4.6
None	No IPR available	PANTHER	PTHR10315:SF4	SEVEN IN ABSENTIA HOMOLOG 4	coord: 4..81 score: 9
None	No IPR available	PANTHER	PTHR10315:SF4	SEVEN IN ABSENTIA HOMOLOG 4	coord: 44..92 score: 1.7

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX570234 ID=EX570234; Name=EX570234; organism=Pisum sativum; type=EST; length=419bp
GATGGATTCACCTTCAATCATCGATACGTCAAAACAAATCCACACGAAGT
TGAAAATGCCACATGGATGCTAACTGTTTTCAACTGTTACGGGCGACATT
TCTGCTTGCACTTTGAGGCTTTCCAGCTTGGCACAGCTCCGGTTTTCATG
GCCTTCTTACGGTTTATGGGCGACGACAACGAGGAGAAGAAATTCAGCTA
CAGCTTAGAAGTCGGTGCAGATGGGCGCAAACTTATATGGCAAGCATTCC
GCGGAGCGTCCGTGACAGTCATAGTAAAGTTAGAGATAGTCAAGATGGAC
TTATAATTCAGAGAAATCAAGCCCTTTATTTTTCTGGCGGTGATAAACAG
GAGTTGAAGTTAAGAATCACGGGTCGAATATGGAAAGAAGATTGAGAAAA
AGGTGTAAATATAACAATG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **Biological Process**
Term	Definition
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0007275	multicellular organism development

Vocabulary: **INTERPRO**
Term	Definition
IPR004162	SINA-like
IPR008974	TRAF-like
IPR013322	TRAF-type
IPR018121	7-in-absentia-prot_TRAF-dom

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