EX570317

EST Overview


Name	EX570317
Unique Name	EX570317
Type	EST
Organism	Pisum sativum (pea)
Sequence length	351

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of EX570317 vs. TrEMBL
Match: B9HS16_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_557395 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.090e-23
Identity = 54/74 (72.97%), Postives = 57/74 (77.03%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KKKQGIQW              AYEREG+ YYSTARLWDDGIIDPADTRKIIG C+SASLNRP E+TKYGVFRM
Sbjct:  500 KKKQGIQWTEEEQENFKSKITEAYEREGNCYYSTARLWDDGIIDPADTRKIIGFCISASLNRPSEDTKYGVFRM 573

[Close]

BLAST of EX570317 vs. TrEMBL
Match: Q56ZH9_ARATH (3-methylcrotonyl-CoA carboxylase non-biotinylated subunit OS=Arabidopsis thaliana GN=At4g34030 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 5.981e-22
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KK+QGI+W              AYERE +PYYSTARLWDDG+IDP DTRK++GLC+SA+LNRP+E+T++GVFRM
Sbjct:   66 KKRQGIKWTEEEEEAFKKKTVDAYEREANPYYSTARLWDDGVIDPCDTRKVLGLCLSAALNRPLEDTRFGVFRM 139

[Close]

BLAST of EX570317 vs. TrEMBL
Match: A2THC0_9SOLN (3-methylcrotonyl-CoA carboxylase (Fragment) OS=Solanum trachycarpum PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.020e-21
Identity = 50/74 (67.57%), Postives = 56/74 (75.68%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KKK+GIQW              AY+REGSPYY+TARLWDDGIIDPADTR+I+ LC+SASLNR  E TKYGVFRM
Sbjct:   57 KKKRGIQWTKEEEEKFKAEVVEAYDREGSPYYATARLWDDGIIDPADTRRILSLCISASLNRGPEATKYGVFRM 130

[Close]

BLAST of EX570317 vs. TrEMBL
Match: A2THB8_9SOLN (3-methylcrotonyl-CoA carboxylase (Fragment) OS=Solanum suaveolens PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.020e-21
Identity = 50/74 (67.57%), Postives = 56/74 (75.68%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KKK+GIQW              AY+REGSPYY+TARLWDDGIIDPADTR+I+ LC+SASLNR  E TKYGVFRM
Sbjct:   57 KKKKGIQWTKEEEEKFKAEVVEAYDREGSPYYATARLWDDGIIDPADTRRILSLCISASLNRGPEATKYGVFRM 130

[Close]

BLAST of EX570317 vs. TrEMBL
Match: A2THB3_9SOLN (3-methylcrotonyl-CoA carboxylase (Fragment) OS=Solanum tabanoense PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.020e-21
Identity = 50/74 (67.57%), Postives = 56/74 (75.68%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KKK+GIQW              AY+REGSPYY+TARLWDDGIIDPADTR+I+ LC+SASLNR  E TKYGVFRM
Sbjct:   57 KKKKGIQWTKEEEEKFKAEVVEAYDREGSPYYATARLWDDGIIDPADTRRILSLCISASLNRGPEATKYGVFRM 130

[Close]

BLAST of EX570317 vs. TrEMBL
Match: A2THB0_9SOLN (3-methylcrotonyl-CoA carboxylase (Fragment) OS=Solanum muricatum PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.020e-21
Identity = 50/74 (67.57%), Postives = 56/74 (75.68%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KKK+GIQW              AY+REGSPYY+TARLWDDGIIDPADTR+I+ LC+SASLNR  E TKYGVFRM
Sbjct:   57 KKKRGIQWTKEEEEKFKAEVVEAYDREGSPYYATARLWDDGIIDPADTRRILSLCISASLNRGPEATKYGVFRM 130

[Close]

BLAST of EX570317 vs. TrEMBL
Match: A2THA6_9SOLN (3-methylcrotonyl-CoA carboxylase (Fragment) OS=Solanum perlongistylum PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.020e-21
Identity = 50/74 (67.57%), Postives = 56/74 (75.68%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KKK+GIQW              AY+REGSPYY+TARLWDDGIIDPADTR+I+ LC+SASLNR  E TKYGVFRM
Sbjct:   57 KKKRGIQWTKEEEEKFKAEVVEAYDREGSPYYATARLWDDGIIDPADTRRILSLCISASLNRGPEATKYGVFRM 130

[Close]

BLAST of EX570317 vs. TrEMBL
Match: A2THA4_9SOLN (3-methylcrotonyl-CoA carboxylase (Fragment) OS=Solanum perlongistylum PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.020e-21
Identity = 50/74 (67.57%), Postives = 56/74 (75.68%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KKK+GIQW              AY+REGSPYY+TARLWDDGIIDPADTR+I+ LC+SASLNR  E TKYGVFRM
Sbjct:   57 KKKRGIQWTKEEEEKFKAEVVEAYDREGSPYYATARLWDDGIIDPADTRRILSLCISASLNRGPEATKYGVFRM 130

[Close]

BLAST of EX570317 vs. TrEMBL
Match: A2THA2_9SOLN (3-methylcrotonyl-CoA carboxylase (Fragment) OS=Solanum catilliflorum PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.020e-21
Identity = 50/74 (67.57%), Postives = 56/74 (75.68%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KKK+GIQW              AY+REGSPYY+TARLWDDGIIDPADTR+I+ LC+SASLNR  E TKYGVFRM
Sbjct:   57 KKKRGIQWTKEEEEKFKAEVVEAYDREGSPYYATARLWDDGIIDPADTRRILSLCISASLNRGPEATKYGVFRM 130

[Close]

BLAST of EX570317 vs. TrEMBL
Match: A2THA1_9SOLN (3-methylcrotonyl-CoA carboxylase (Fragment) OS=Solanum caripense PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.020e-21
Identity = 50/74 (67.57%), Postives = 56/74 (75.68%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KKK+GIQW              AY+REGSPYY+TARLWDDGIIDPADTR+I+ LC+SASLNR  E TKYGVFRM
Sbjct:   57 KKKRGIQWTKEEEEKFKAEVVAAYDREGSPYYATARLWDDGIIDPADTRRILSLCISASLNRGPEATKYGVFRM 130

[Close]

BLAST of EX570317 vs. SwissProt
Match: MCCB_ARATH (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Arabidopsis thaliana GN=MCCB PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 3.015e-23
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KK+QGI+W              AYERE +PYYSTARLWDDG+IDP DTRK++GLC+SA+LNRP+E+T++GVFRM
Sbjct:  514 KKRQGIKWTEEEEEAFKKKTVDAYEREANPYYSTARLWDDGVIDPCDTRKVLGLCLSAALNRPLEDTRFGVFRM 587

[Close]

BLAST of EX570317 vs. SwissProt
Match: MCCB_CAEEL (Probable methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Caenorhabditis elegans GN=F02A9.4 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.602e-16
Identity = 35/74 (47.30%), Postives = 49/74 (66.22%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KK++G  W               +E+EG PY+++ARLWDDG+IDP DTRK++GL   ++L +PI  TK+GVFRM
Sbjct:  535 KKREGADWTDQQDLELRKPVEEKFEKEGHPYFASARLWDDGVIDPKDTRKVLGLAFQSTLQKPIPETKFGVFRM 608

[Close]

BLAST of EX570317 vs. SwissProt
Match: MCCB_MOUSE (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Mus musculus GN=Mccc2 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 3.569e-16
Identity = 37/74 (50.00%), Postives = 51/74 (68.92%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            K ++G Q++              +E EG+PYYS+ARLWDDGIIDP DTR ++GL +SA+LN PI+ T +G+FRM
Sbjct:  490 KAREGKQFSSAEEAALKEPIIKRFEEEGNPYYSSARLWDDGIIDPVDTRLVLGLSLSAALNAPIQRTDFGIFRM 563

[Close]

BLAST of EX570317 vs. SwissProt
Match: MCCB_RAT (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Rattus norvegicus GN=Mccc2 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 4.662e-16
Identity = 36/74 (48.65%), Postives = 51/74 (68.92%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            + ++G Q++              +E EG+PYYS+ARLWDDGIIDP DTR ++GL +SA+LN PI+ T +G+FRM
Sbjct:  490 RAREGKQFSSAEEAALKEPIIKRFEEEGNPYYSSARLWDDGIIDPVDTRLVLGLSISAALNAPIQRTDFGIFRM 563

[Close]

BLAST of EX570317 vs. SwissProt
Match: MCCB_HUMAN (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Homo sapiens GN=MCCC2 PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 4.662e-16
Identity = 37/74 (50.00%), Postives = 51/74 (68.92%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            + ++G Q++              +E EG+PYYS+AR+WDDGIIDPADTR ++GL  SA+LN PIE T +G+FRM
Sbjct:  490 RAREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM 563

[Close]

BLAST of EX570317 vs. SwissProt
Match: MCCB_DROME (Probable methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Drosophila melanogaster GN=L(2)04524 PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 1.771e-15
Identity = 38/74 (51.35%), Postives = 51/74 (68.92%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            +K+ G +++              +E EGSPYYSTARLWDDGIIDPA+TR+I+GL + A+LN   + TK+GVFRM
Sbjct:  505 RKRAGKEFSEEEAQKLKAPIVEMFEAEGSPYYSTARLWDDGIIDPANTRQILGLSLKAALNNAGQETKFGVFRM 578

[Close]

BLAST of EX570317 vs. SwissProt
Match: MCCB_DICDI (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Dictyostelium discoideum GN=mccb PE=3 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 3.127e-12
Identity = 34/74 (45.95%), Postives = 46/74 (62.16%), Query Frame = 2

Query:    8 KQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIE--NTKYGVFRM 223
            K+  QW+              +E EGS YYS+AR WDDG+IDP D+RK+I L +SA +N+PI   +  +GVFRM
Sbjct:  515 KENKQWSPEEENTFKKPISDKFEEEGSIYYSSARCWDDGVIDPQDSRKVIALSLSACMNQPINPPSDGFGVFRM 588

[Close]

BLAST of EX570317 vs. TAIR peptide
Match: AT4G34030.1 (| Symbols: MCCB | 3-methylcrotonyl-CoA carboxylase | chr4:16301298-16303949 FORWARD LENGTH=587)

HSP 1 Score: 107.071 bits (266), Expect = 2.318e-24
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = 2

Query:    2 KKKQGIQWNXXXXXXXXXXXXXAYEREGSPYYSTARLWDDGIIDPADTRKIIGLCVSASLNRPIENTKYGVFRM 223
            KK+QGI+W              AYERE +PYYSTARLWDDG+IDP DTRK++GLC+SA+LNRP+E+T++GVFRM
Sbjct:  514 KKRQGIKWTEEEEEAFKKKTVDAYEREANPYYSTARLWDDGVIDPCDTRKVLGLCLSAALNRPLEDTRFGVFRM 587

The following BLAST results are available for this feature:

BLAST of EX570317 vs. TrEMBL
BLAST of EX570317 vs. SwissProt
BLAST of EX570317 vs. TAIR peptide

BLAST of EX570317 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HS16_POPTR	1.090e-23	72.97	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q56ZH9_ARATH	5.981e-22	62.16	3-methylcrotonyl-CoA carboxylase non-biotinylated ...	[more]
A2THC0_9SOLN	1.020e-21	67.57	3-methylcrotonyl-CoA carboxylase (Fragment) OS=Sol...	[more]
A2THB8_9SOLN	1.020e-21	67.57	3-methylcrotonyl-CoA carboxylase (Fragment) OS=Sol...	[more]
A2THB3_9SOLN	1.020e-21	67.57	3-methylcrotonyl-CoA carboxylase (Fragment) OS=Sol...	[more]
A2THB0_9SOLN	1.020e-21	67.57	3-methylcrotonyl-CoA carboxylase (Fragment) OS=Sol...	[more]
A2THA6_9SOLN	1.020e-21	67.57	3-methylcrotonyl-CoA carboxylase (Fragment) OS=Sol...	[more]
A2THA4_9SOLN	1.020e-21	67.57	3-methylcrotonyl-CoA carboxylase (Fragment) OS=Sol...	[more]
A2THA2_9SOLN	1.020e-21	67.57	3-methylcrotonyl-CoA carboxylase (Fragment) OS=Sol...	[more]
A2THA1_9SOLN	1.020e-21	67.57	3-methylcrotonyl-CoA carboxylase (Fragment) OS=Sol...	[more]

BLAST of EX570317 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
MCCB_ARATH	3.015e-23	62.16	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]
MCCB_CAEEL	1.602e-16	47.30	Probable methylcrotonoyl-CoA carboxylase beta chai...	[more]
MCCB_MOUSE	3.569e-16	50.00	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]
MCCB_RAT	4.662e-16	48.65	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]
MCCB_HUMAN	4.662e-16	50.00	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]
MCCB_DROME	1.771e-15	51.35	Probable methylcrotonoyl-CoA carboxylase beta chai...	[more]
MCCB_DICDI	3.127e-12	45.95	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]

BLAST of EX570317 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G34030.1	2.318e-24	62.16	\| Symbols: MCCB \| 3-methylcrotonyl-CoA carboxylase...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000022	Carboxyl transferase	PFAM	PF01039	Carboxyl_trans	coord: 5..66 score: 5.7
None	No IPR available	GENE3D	3.90.226.10	no description	coord: 3..67 score: 8.8
None	No IPR available	PANTHER	PTHR22855	ACETYL, PROPIONYL, PYRUVATE, AND GLUTACONYL CARBOXYLASE-RELATED	coord: 7..74 score: 3.2
None	No IPR available	PANTHER	PTHR22855:SF13	ACETYL-COA CARBOXYLASE CARBOXYLTRANSFERASE-RELATED	coord: 7..74 score: 3.2
None	No IPR available	SEG	seg	seg	coord: 10..22 score
None	No IPR available	SUPERFAMILY	52096	ClpP/crotonase	coord: 4..69 score: 5.4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX570317 ID=EX570317; Name=EX570317; organism=Pisum sativum; type=EST; length=351bp
TAAGAAAAAGCAAGGAATTCAGTGGAACAAGGAGGAGGAAGAAAAGTTCA
AAAGAAAAGTAGTTGAGGCGTACGAGAGAGAAGGAAGTCCTTATTACTCA
ACGGCGAGGCTTTGGGATGATGGAATTATTGATCCAGCTGATACAAGAAA
AATAATTGGTCTATGTGTTTCAGCTTCCTTGAACCGTCCCATAGAAAATA
CCAAATATGGTGTATTTAGAATGTGAAGCTTTGTTGTGTGCCAAATGTTG
TTAAAGTCTGTTGTAGATTTGTTAAGAAATGGAAGAGAGGAAGGGGGGTG
TTGAATGGATAAACATGTAAAACGGGATGAGTGAAAAGTGAATTCCTTCT
T

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016874	ligase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000022	Carboxyl_trans

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