AM161719

EST Overview


Name	AM161719
Unique Name	AM161719
Type	EST
Organism	Pisum sativum (pea)
Sequence length	353

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM161719 vs. TrEMBL
Match: Q5K3Q6_SOYBN (Delta 1-pyrroline-5-carboxylate synthetase (Fragment) OS=Glycine max GN=p5cs PE=4 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 5.350e-47
Identity = 96/117 (82.05%), Postives = 100/117 (85.47%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+ITS MPDTVG KLIGLVTSRDEIP            VPRGSNKLVSQIKEST+IPVLGHADGICH+YVDKSAN DMAK IVRDAKTDYPAACNA+ETLL
Sbjct:   27 LLKGGKEARRSNAILHKVITSVMPDTVGDKLIGLVTSRDEIPDLLKLDDVIDLVVPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANFDMAKQIVRDAKTDYPAACNAMETLL 143

[Close]

BLAST of AM161719 vs. TrEMBL
Match: Q9AYM4_VIGUN (VuP5CS protein OS=Vigna unguiculata GN=VuP5CS PE=2 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 1.457e-44
Identity = 91/117 (77.78%), Postives = 99/117 (84.62%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA+RSNA LHK+ITS +P+TVG KLIGLV SRDEIP            VPRGSNKLVSQIKEST+IPVLGHADGICH+YVDKSA IDMAK I+RDAKTDYPAACNA+ETLL
Sbjct:  468 LLKGGKEAQRSNAILHKVITSVIPNTVGDKLIGLVNSRDEIPYLLKLDDVIDLVVPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSAKIDMAKQIIRDAKTDYPAACNAMETLL 584

[Close]

BLAST of AM161719 vs. TrEMBL
Match: C7G4C3_VIGUN (Delta 1-pyrroline-5-carboxylate synthase OS=Vigna unguiculata GN=VuP5CS PE=2 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 1.457e-44
Identity = 91/117 (77.78%), Postives = 99/117 (84.62%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA+RSNA LHK+ITS +P+TVG KLIGLV SRDEIP            VPRGSNKLVSQIKEST+IPVLGHADGICH+YVDKSA IDMAK I+RDAKTDYPAACNA+ETLL
Sbjct:  468 LLKGGKEAQRSNAILHKVITSVIPNTVGDKLIGLVNSRDEIPYLLKLDDVIDLVVPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSAKIDMAKQIIRDAKTDYPAACNAMETLL 584

[Close]

BLAST of AM161719 vs. TrEMBL
Match: D7TLM5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_19.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016467001 PE=4 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 7.231e-44
Identity = 88/117 (75.21%), Postives = 99/117 (84.62%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA+RSNA LHK+IT A+PD+VG KLIGLVTSR+EIP            +PRGSNKLVSQIK+ST+IPVLGHADGICH+YVDKSAN+D AKHIV DAK DYPAACNA+ETLL
Sbjct:  436 LLKGGKEAKRSNAILHKVITEAIPDSVGKKLIGLVTSREEIPNLLKLDDVIDLVIPRGSNKLVSQIKDSTKIPVLGHADGICHVYVDKSANMDTAKHIVLDAKVDYPAACNAMETLL 552

[Close]

BLAST of AM161719 vs. TrEMBL
Match: D7THH7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00034097001 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 9.443e-44
Identity = 87/117 (74.36%), Postives = 99/117 (84.62%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA RSNA LHK+IT A+PDTVG KLIGLVT+R++IP            +PRGSNKLVSQIK+ST+IPVLGHADGICH+Y+DKSAN+ MAKHIV DAKTDYPAACNA+ETLL
Sbjct:  431 LLKGGKEANRSNAILHKVITEAIPDTVGEKLIGLVTTREQIPDLLKLDDVIDLVIPRGSNKLVSQIKDSTKIPVLGHADGICHVYIDKSANMKMAKHIVLDAKTDYPAACNAMETLL 547

[Close]

BLAST of AM161719 vs. TrEMBL
Match: Q5K3Q5_LACSA (Delta 1-pyrroline-5-carboxylate synthetase (Fragment) OS=Lactuca sativa GN=p5cs PE=4 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 3.589e-43
Identity = 86/117 (73.50%), Postives = 101/117 (86.32%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA+RSNA LHK+ITSA+P+TVG+KLIGL+TSR+EIP            +PRGSNKLVSQIK ST+IPVLGH+DGICH+YVDK+AN++MAK IV DAKTDYPAACNA+ETLL
Sbjct:   29 LLKGGKEAKRSNAILHKVITSALPETVGTKLIGLLTSREEIPDLLRLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHSDGICHVYVDKAANLEMAKKIVLDAKTDYPAACNAMETLL 145

[Close]

BLAST of AM161719 vs. TrEMBL
Match: B9HT60_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_833794 PE=4 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 4.687e-43
Identity = 86/117 (73.50%), Postives = 99/117 (84.62%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA+RSNA LHK+IT+A+PDTVG +LIGLVTSRDEIP            +PRGSNKLVSQIK ST+IPVLGHADGICH+Y+DKSAN++MAK +V DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEAKRSNAILHKVITTAIPDTVGGRLIGLVTSRDEIPDLLKLDDVIDLVIPRGSNKLVSQIKSSTKIPVLGHADGICHVYMDKSANMEMAKRVVLDAKIDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. TrEMBL
Match: B1NJ24_OPUST (Delta 1-pyrroline-5-carboxylate synthetase (Fragment) OS=Opuntia streptacantha GN=P5CS PE=2 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 6.121e-43
Identity = 88/117 (75.21%), Postives = 98/117 (83.76%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA+RSNA LHK+ITSA+P  VG KLIGLVTSR+EIP            +PRGSNKLVSQIKEST+IPVLGHADGICH+YVDKSAN+DMA+ IV DAK DYPAACNA+ETLL
Sbjct:  347 LLKGGKEAKRSNAILHKVITSAIPGKVGEKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANMDMARRIVLDAKMDYPAACNAMETLL 463

[Close]

BLAST of AM161719 vs. TrEMBL
Match: A5GZ76_9ERIC (Pyrroline-5-carboxylate synthetase OS=Aegiceras corniculatum GN=P5CS PE=2 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 6.121e-43
Identity = 89/117 (76.07%), Postives = 98/117 (83.76%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+ITSA+P+ VG KLIGLVTSRDEIP            +PRGSNKLVSQIKEST+IPVLGHADGICH+YVDKSA++  AK IV DAKTDYPAACNA+ETLL
Sbjct:  434 LLKGGKEARRSNAALHKVITSAIPENVGQKLIGLVTSRDEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSADMGKAKGIVLDAKTDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. TrEMBL
Match: Q9AXN4_BRANA (Delta 1-pyrroline-5-carboxylate synthetase A OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 7.994e-43
Identity = 86/117 (73.50%), Postives = 97/117 (82.91%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+IT A+P+TVG KLIGLVTSR+EIP            +PRGSNKLVSQIK +T+IPVLGHADGICH+YVDK+ N+DMAK IV DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNTTKIPVLGHADGICHVYVDKACNVDMAKRIVSDAKLDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. SwissProt
Match: P5CS_MESCR (Delta-1-pyrroline-5-carboxylate synthase OS=Mesembryanthemum crystallinum GN=P5CS PE=2 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 6.645e-47
Identity = 93/117 (79.49%), Postives = 100/117 (85.47%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA+RSNA LHK+ITSA+PD VG KLIGLVTSRDEIP            +PRGSNKLVSQIKESTRIPVLGHADGICH+YVDKSAN+DMAK IV DAKTDYPAACNA+ETLL
Sbjct:  431 LLKGGKEAKRSNAILHKVITSAIPDKVGEKLIGLVTSRDEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTRIPVLGHADGICHVYVDKSANMDMAKRIVLDAKTDYPAACNAMETLL 547

[Close]

BLAST of AM161719 vs. SwissProt
Match: P5CS_ACTDE (Delta-1-pyrroline-5-carboxylate synthase OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 1.061e-44
Identity = 87/117 (74.36%), Postives = 100/117 (85.47%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            +LKGGKEA+RSNA LHK+ITSA+P+ VG +LIGLVTSR+EIP            +PRGSNKLVSQIKEST+IPVLGHADGICH+YVDKSAN+DMAK +V DAKTDYPAACNA+ETLL
Sbjct:  434 VLKGGKEAKRSNAILHKVITSAIPENVGPRLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANMDMAKKVVLDAKTDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. SwissProt
Match: P5CS2_ARATH (Delta-1-pyrroline-5-carboxylate synthase B OS=Arabidopsis thaliana GN=P5CSB PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 5.265e-44
Identity = 87/117 (74.36%), Postives = 96/117 (82.05%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+IT A+P+TVG KLIGLVTSR+EIP            +PRGSNKLVSQIK ST+IPVLGHADGICH+YVDKS  +DMAK IV DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. SwissProt
Match: P5CS_VIGAC (Delta-1-pyrroline-5-carboxylate synthase OS=Vigna aconitifolia PE=2 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 1.173e-43
Identity = 86/117 (73.50%), Postives = 96/117 (82.05%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA+RSNA LHK+I  A+PD VG KLIGLVTSR+EIP            +PRGSNKLVSQIK ST+IPVLGHADGICH+YVDKSAN++MAK IV DAK DYPAACNA+ETLL
Sbjct:  435 LLKGGKEAKRSNAILHKVIIEAIPDNVGGKLIGLVTSREEIPELLKLDDVIDLVIPRGSNKLVSQIKSSTKIPVLGHADGICHVYVDKSANVEMAKRIVLDAKVDYPAACNAMETLL 551

[Close]

BLAST of AM161719 vs. SwissProt
Match: P5CS1_ARATH (Delta-1-pyrroline-5-carboxylate synthase A OS=Arabidopsis thaliana GN=P5CSA PE=1 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 9.929e-43
Identity = 84/117 (71.79%), Postives = 96/117 (82.05%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+IT A+P+TVG KLIGLVTSR+EIP            +PRGSNKLV+QIK +T+IPVLGHADGICH+YVDK+ + DMAK IV DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. SwissProt
Match: P5CS_SOLLC (Delta-1-pyrroline-5-carboxylate synthase OS=Solanum lycopersicum GN=PRO2 PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 3.773e-42
Identity = 84/117 (71.79%), Postives = 97/117 (82.91%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            +LKGGKEA+RSNA LHK+ITSA+P +VG +LIGLVTSR+EIP            +PRGSNKLVSQIK ST+IPVLGHADGICH+YVDKSA++DMAK I  DAK DYPAACNA+ETLL
Sbjct:  434 MLKGGKEAKRSNAILHKVITSAIPVSVGERLIGLVTSREEIPELLKLDDVIDLVIPRGSNKLVSQIKASTKIPVLGHADGICHVYVDKSADMDMAKRITVDAKIDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. SwissProt
Match: P5CS_ORYSJ (Delta-1-pyrroline-5-carboxylate synthase OS=Oryza sativa subsp. japonica GN=P5CS PE=2 SV=2)

HSP 1 Score: 169.088 bits (427), Expect = 6.436e-42
Identity = 84/117 (71.79%), Postives = 94/117 (80.34%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEA RSN  LHK+IT A+P  VG KLIGLVT+RDEI             +PRGSNKLVSQIK ST+IPVLGHADGICH+Y+DKSA++DMAKHIV DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEAIRSNTILHKVITDAIPRNVGEKLIGLVTTRDEIADLLKLDDVIDLVIPRGSNKLVSQIKASTKIPVLGHADGICHVYIDKSADMDMAKHIVMDAKIDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. SwissProt
Match: PROA_SYNY3 (Probable gamma-glutamyl phosphate reductase OS=Synechocystis sp. (strain PCC 6803) GN=proA PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 3.209e-25
Identity = 60/118 (50.85%), Postives = 81/118 (68.64%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGS-KLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            +LKGG+EA RS   L ++I +A+  TV S + I L+T+R+EI             +PRGSN+ V  I+++T+IPVLGHADGICH+Y+D  A++  A  I  DAKT YPAACNA+ETLL
Sbjct:  149 ILKGGREATRSCQVLTEVIQTALAKTVVSPEAINLLTTREEIRELLGLNQYVDLIIPRGSNEFVQYIQQNTQIPVLGHADGICHLYLDAQADLSKAIPITVDAKTQYPAACNAIETLL 266

[Close]

BLAST of AM161719 vs. SwissProt
Match: PROA_SYNP2 (Gamma-glutamyl phosphate reductase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=proA PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 5.474e-25
Identity = 59/118 (50.00%), Postives = 80/118 (67.80%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDT-VGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            +LKGGKEA +S   L +II +A+ DT V  + + L+T+R+EI             +PRGSN  V  ++++T IPVLGHADG+CH+YVD +A++     IV DAKT YPAACNA+ETLL
Sbjct:  144 ILKGGKEAIQSCTVLTEIIQTALQDTAVSPQAVTLLTTREEIKTLLSLDQYVDLIIPRGSNAFVQYVQQNTTIPVLGHADGVCHLYVDVAADLSKTIPIVVDAKTQYPAACNAVETLL 261

[Close]

BLAST of AM161719 vs. SwissProt
Match: PROA_MICAN (Gamma-glutamyl phosphate reductase OS=Microcystis aeruginosa (strain NIES-843) GN=proA PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.219e-24
Identity = 60/118 (50.85%), Postives = 75/118 (63.56%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDT-VGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            +LKGGKEA RS   L  II  A+  T V    + L+T+R+EI             +PRGSN+ V  I+ +TRIPVLGHADGICH+Y+DK A++  A  I  D KT YPAACN +ETLL
Sbjct:  147 ILKGGKEAIRSCQALVTIIHQALSQTKVNPAAVRLLTTREEIQELLKLDQYIDLIIPRGSNEFVRYIQNNTRIPVLGHADGICHLYIDKEADLSKAIRITVDGKTQYPAACNTIETLL 264

[Close]

BLAST of AM161719 vs. TAIR peptide
Match: AT3G55610.2 (| Symbols: P5CS2 | delta 1-pyrroline-5-carboxylate synthase 2 | chr3:20625005-20628989 REVERSE LENGTH=622)

HSP 1 Score: 176.022 bits (445), Expect = 4.052e-45
Identity = 87/117 (74.36%), Postives = 96/117 (82.05%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+IT A+P+TVG KLIGLVTSR+EIP            +PRGSNKLVSQIK ST+IPVLGHADGICH+YVDKS  +DMAK IV DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. TAIR peptide
Match: AT3G55610.1 (| Symbols: P5CS2 | delta 1-pyrroline-5-carboxylate synthase 2 | chr3:20624278-20628989 REVERSE LENGTH=726)

HSP 1 Score: 176.022 bits (445), Expect = 4.052e-45
Identity = 87/117 (74.36%), Postives = 96/117 (82.05%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+IT A+P+TVG KLIGLVTSR+EIP            +PRGSNKLVSQIK ST+IPVLGHADGICH+YVDKS  +DMAK IV DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. TAIR peptide
Match: AT2G39800.4 (| Symbols: P5CS1 | delta1-pyrroline-5-carboxylate synthase 1 | chr2:16598516-16602939 REVERSE LENGTH=717)

HSP 1 Score: 171.785 bits (434), Expect = 7.641e-44
Identity = 84/117 (71.79%), Postives = 96/117 (82.05%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+IT A+P+TVG KLIGLVTSR+EIP            +PRGSNKLV+QIK +T+IPVLGHADGICH+YVDK+ + DMAK IV DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. TAIR peptide
Match: AT2G39800.1 (| Symbols: P5CS1, ATP5CS | delta1-pyrroline-5-carboxylate synthase 1 | chr2:16598516-16602939 REVERSE LENGTH=717)

HSP 1 Score: 171.785 bits (434), Expect = 7.641e-44
Identity = 84/117 (71.79%), Postives = 96/117 (82.05%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+IT A+P+TVG KLIGLVTSR+EIP            +PRGSNKLV+QIK +T+IPVLGHADGICH+YVDK+ + DMAK IV DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLL 550

[Close]

BLAST of AM161719 vs. TAIR peptide
Match: AT2G39800.2 (| Symbols: P5CS1, ATP5CS | delta1-pyrroline-5-carboxylate synthase 1 | chr2:16598516-16601976 REVERSE LENGTH=614)

HSP 1 Score: 171.785 bits (434), Expect = 7.641e-44
Identity = 84/117 (71.79%), Postives = 96/117 (82.05%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+IT A+P+TVG KLIGLVTSR+EIP            +PRGSNKLV+QIK +T+IPVLGHADGICH+YVDK+ + DMAK IV DAK DYPAACNA+ETLL
Sbjct:  331 LLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLL 447

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BLAST of AM161719 vs. TAIR peptide
Match: AT2G39800.3 (| Symbols: P5CS1 | delta1-pyrroline-5-carboxylate synthase 1 | chr2:16598516-16602939 REVERSE LENGTH=714)

HSP 1 Score: 159.458 bits (402), Expect = 3.924e-40
Identity = 81/117 (69.23%), Postives = 93/117 (79.49%), Query Frame = -1

Query:    3 LLKGGKEARRSNAXLHKIITSAMPDTVGSKLIGLVTSRDEIPXXXXXXXXXXXXVPRGSNKLVSQIKESTRIPVLGHADGICHIYVDKSANIDMAKHIVRDAKTDYPAACNALETLL 353
            LLKGGKEARRSNA LHK+IT A+P+TVG KLIGLVTSR+EIP            +PRGSNKLV+QIK +T+IPVL   DGICH+YVDK+ + DMAK IV DAK DYPAACNA+ETLL
Sbjct:  434 LLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVL---DGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLL 547

The following BLAST results are available for this feature:

BLAST of AM161719 vs. TrEMBL
BLAST of AM161719 vs. SwissProt
BLAST of AM161719 vs. TAIR peptide

BLAST of AM161719 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q5K3Q6_SOYBN	5.350e-47	82.05	Delta 1-pyrroline-5-carboxylate synthetase (Fragme...	[more]
Q9AYM4_VIGUN	1.457e-44	77.78	VuP5CS protein OS=Vigna unguiculata GN=VuP5CS PE=2...	[more]
C7G4C3_VIGUN	1.457e-44	77.78	Delta 1-pyrroline-5-carboxylate synthase OS=Vigna ...	[more]
D7TLM5_VITVI	7.231e-44	75.21	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7THH7_VITVI	9.443e-44	74.36	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q5K3Q5_LACSA	3.589e-43	73.50	Delta 1-pyrroline-5-carboxylate synthetase (Fragme...	[more]
B9HT60_POPTR	4.687e-43	73.50	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B1NJ24_OPUST	6.121e-43	75.21	Delta 1-pyrroline-5-carboxylate synthetase (Fragme...	[more]
A5GZ76_9ERIC	6.121e-43	76.07	Pyrroline-5-carboxylate synthetase OS=Aegiceras co...	[more]
Q9AXN4_BRANA	7.994e-43	73.50	Delta 1-pyrroline-5-carboxylate synthetase A OS=Br...	[more]

BLAST of AM161719 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
P5CS_MESCR	6.645e-47	79.49	Delta-1-pyrroline-5-carboxylate synthase OS=Mesemb...	[more]
P5CS_ACTDE	1.061e-44	74.36	Delta-1-pyrroline-5-carboxylate synthase OS=Actini...	[more]
P5CS2_ARATH	5.265e-44	74.36	Delta-1-pyrroline-5-carboxylate synthase B OS=Arab...	[more]
P5CS_VIGAC	1.173e-43	73.50	Delta-1-pyrroline-5-carboxylate synthase OS=Vigna ...	[more]
P5CS1_ARATH	9.929e-43	71.79	Delta-1-pyrroline-5-carboxylate synthase A OS=Arab...	[more]
P5CS_SOLLC	3.773e-42	71.79	Delta-1-pyrroline-5-carboxylate synthase OS=Solanu...	[more]
P5CS_ORYSJ	6.436e-42	71.79	Delta-1-pyrroline-5-carboxylate synthase OS=Oryza ...	[more]
PROA_SYNY3	3.209e-25	50.85	Probable gamma-glutamyl phosphate reductase OS=Syn...	[more]
PROA_SYNP2	5.474e-25	50.00	Gamma-glutamyl phosphate reductase OS=Synechococcu...	[more]
PROA_MICAN	1.219e-24	50.85	Gamma-glutamyl phosphate reductase OS=Microcystis ...	[more]

BLAST of AM161719 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT3G55610.2	4.052e-45	74.36	\| Symbols: P5CS2 \| delta 1-pyrroline-5-carboxylate...	[more]
AT3G55610.1	4.052e-45	74.36	\| Symbols: P5CS2 \| delta 1-pyrroline-5-carboxylate...	[more]
AT2G39800.4	7.641e-44	71.79	\| Symbols: P5CS1 \| delta1-pyrroline-5-carboxylate ...	[more]
AT2G39800.1	7.641e-44	71.79	\| Symbols: P5CS1, ATP5CS \| delta1-pyrroline-5-carb...	[more]
AT2G39800.2	7.641e-44	71.79	\| Symbols: P5CS1, ATP5CS \| delta1-pyrroline-5-carb...	[more]
AT2G39800.3	3.924e-40	69.23	\| Symbols: P5CS1 \| delta1-pyrroline-5-carboxylate ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 8..27 score
None	No IPR available	PANTHER	PTHR11063	GLUTAMATE SEMIALDEHYDE DEHYDROGENASE	coord: 1..118 score: 2.3
None	No IPR available	PANTHER	PTHR11063:SF2	DELTA 1-PYRROLINE-5-CARBOXYLATE SYNTHETASE	coord: 1..118 score: 2.3
None	No IPR available	SEG	seg	seg	coord: 43..55 score
IPR016161	Aldehyde/histidinol dehydrogenase	SUPERFAMILY	53720	ALDH-like	coord: 1..118 score: 1.2
IPR016162	Aldehyde dehydrogenase, N-terminal	GENE3D	3.40.605.10	no description	coord: 1..86 score: 1.6
IPR016163	Aldehyde dehydrogenase, C-terminal	GENE3D	3.40.309.10	no description	coord: 87..118 score: 3.9

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM161719 ID=AM161719; Name=AM161719; organism=Pisum sativum; type=EST; length=353bp
ACAAGAAGAGTCTCCAATGCATTGCAAGCTGCAGGGTAATCAGTCTTTGC
ATCCCTAACTATATGCTTTGCCATATCAATATTAGCAGACTTGTCAACAT
AGATATGACAAATTCCATCAGCATGACCAAGAACAGGGATTCTTGTTGAC
TCCTTGATTTGAGAAACCAGCTTATTACTGCCTCTAGGGACCACAAGATC
TATCACATCATCAAGCTTGAGTAGATCTGGAATTTCATCTCTTGAAGTGA
CAAGCCCGATAAGTTTGCTACCGACTGTATCTGGCATGGCTGAAGTAATG
ATCTTGTGTAANATCGCATTTGATCTTCGGGCTTCCTTTCCTCCTTTAAG
TAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR016161	Ald_DH/histidinol_DH
IPR016162	Ald_DH_N
IPR016163	Ald_DH_C

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