AM161962

EST Overview
NameAM161962
Unique NameAM161962
TypeEST
OrganismPisum sativum (pea)
Sequence length215
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v2Pisum sativum unigene v2EST
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of AM161962 vs. TrEMBL
Match: B9SJ24_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0597980 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.342e-11
Identity = 33/50 (66.00%), Postives = 43/50 (86.00%), Query Frame = 3 
Query:    6 AAMLMSSSLSHVRPLTCASS-SRLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            A+ ++ +SL H RPLTCA+S +RLVSHP DL+KWV+ EGGFVH+AVKI+Q
Sbjct:    3 ASKMVMASLIHRRPLTCAASITRLVSHPPDLIKWVRHEGGFVHQAVKISQ 52
[Close]
BLAST of AM161962 vs. TrEMBL
Match: D7KAL1_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472886 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.875e-8
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3 
Query:   12 MLMSSSLSHVRPLTCASSS---RLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            M M+S  + +RP TC ++S   RL  HP DL++W+KREGGFVH A+K++Q
Sbjct:    1 MAMASLAAQIRPFTCLAASQPSRLAPHPPDLIRWIKREGGFVHHAIKLSQ 50
[Close]
BLAST of AM161962 vs. TrEMBL
Match: B9HJG0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_766182 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 5.061e-8
Identity = 32/71 (45.07%), Postives = 46/71 (64.79%), Query Frame = 3 
Query:   24 SSLSHVRPLTCASS-------SRLVSHPHDLVKWVKREGGFVHRAVKIAQPDSXXXXXXXXXVAKEEIPIG 215
            +SL++ RPLTCA++        RLV +P DL+KWV+REGGFVH+A+KI+Q  +         +A  +IP G
Sbjct:    9 ASLTNCRPLTCAATFTASSYQERLVPNPPDLIKWVRREGGFVHQAIKISQDGT----NGPGLIASHDIPKG 75
[Close]
BLAST of AM161962 vs. TrEMBL
Match: Q9FYK3_ARATH (F21J9.27 OS=Arabidopsis thaliana GN=At1g24610 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 8.633e-8
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3 
Query:   12 MLMSSSLSHVRPLTCASSS---RLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            M ++S  + +RP TC ++S   RL  HP DL++W+KREGGFVH AVK++Q
Sbjct:    6 MAIASLAAQIRPFTCLAASLPSRLAPHPPDLIRWIKREGGFVHHAVKLSQ 55
[Close]
BLAST of AM161962 vs. TrEMBL
Match: Q8L9I6_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 8.633e-8
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3 
Query:   12 MLMSSSLSHVRPLTCASSS---RLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            M ++S  + +RP TC ++S   RL  HP DL++W+KREGGFVH AVK++Q
Sbjct:    1 MAIASLAAQIRPFTCLAASLPSRLAPHPPDLIRWIKREGGFVHHAVKLSQ 50
[Close]
BLAST of AM161962 vs. TrEMBL
Match: D7T8L6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011666001 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.127e-7
Identity = 37/80 (46.25%), Postives = 47/80 (58.75%), Query Frame = 3 
Query:    6 AAMLMSSSLSHVRPLTCASSS---------RLVSHPHDLVKWVKREGGFVHRAVKIA-QPDSXXXXXXXXXVAKEEIPIG 215
            A +  S  L+  RPLTCA+++         RLV HP DL+KWV+REGGFVH+AV IA   DS         VA ++IP G
Sbjct:    2 AGLGASKILTQFRPLTCAAATATATACHPGRLVPHPPDLIKWVRREGGFVHQAVTIAPSADS----CGLGLVASQDIPKG 77
[Close]
BLAST of AM161962 vs. TAIR peptide
Match: AT1G24610.1 (| Symbols: | Rubisco methyltransferase family protein | chr1:8720906-8722711 REVERSE LENGTH=476)

HSP 1 Score: 60.077 bits (144), Expect = 3.316e-10
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3 
Query:   12 MLMSSSLSHVRPLTCASSS---RLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            M ++S  + +RP TC ++S   RL  HP DL++W+KREGGFVH AVK++Q
Sbjct:    6 MAIASLAAQIRPFTCLAASLPSRLAPHPPDLIRWIKREGGFVHHAVKLSQ 55
[Close]
BLAST of AM161962 vs. TAIR peptide
Match: AT1G24610.1 (| Symbols: | Rubisco methyltransferase family protein | chr1:8720906-8722711 REVERSE LENGTH=476)

HSP 1 Score: 60.077 bits (144), Expect = 3.316e-10
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3 
Query:   12 MLMSSSLSHVRPLTCASSS---RLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            M ++S  + +RP TC ++S   RL  HP DL++W+KREGGFVH AVK++Q
Sbjct:    6 MAIASLAAQIRPFTCLAASLPSRLAPHPPDLIRWIKREGGFVHHAVKLSQ 55
[Close]
BLAST of AM161962 vs. TrEMBL
Match: B9SJ24_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0597980 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.043e-10
Identity = 33/50 (66.00%), Postives = 43/50 (86.00%), Query Frame = 3 
Query:    6 AAMLMSSSLSHVRPLTCASS-SRLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            A+ ++ +SL H RPLTCA+S +RLVSHP DL+KWV+ EGGFVH+AVKI+Q
Sbjct:    3 ASKMVMASLIHRRPLTCAASITRLVSHPPDLIKWVRHEGGFVHQAVKISQ 52
[Close]
BLAST of AM161962 vs. TrEMBL
Match: D7KAL1_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472886 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.846e-8
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3 
Query:   12 MLMSSSLSHVRPLTCASSS---RLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            M M+S  + +RP TC ++S   RL  HP DL++W+KREGGFVH A+K++Q
Sbjct:    1 MAMASLAAQIRPFTCLAASQPSRLAPHPPDLIRWIKREGGFVHHAIKLSQ 50
[Close]
BLAST of AM161962 vs. TrEMBL
Match: B9HJG0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_766182 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 6.329e-8
Identity = 32/71 (45.07%), Postives = 46/71 (64.79%), Query Frame = 3 
Query:   24 SSLSHVRPLTCASS-------SRLVSHPHDLVKWVKREGGFVHRAVKIAQPDSXXXXXXXXXVAKEEIPIG 215
            +SL++ RPLTCA++        RLV +P DL+KWV+REGGFVH+A+KI+Q  +         +A  +IP G
Sbjct:    9 ASLTNCRPLTCAATFTASSYQERLVPNPPDLIKWVRREGGFVHQAIKISQDGT----NGPGLIASHDIPKG 75
[Close]
BLAST of AM161962 vs. TrEMBL
Match: Q9FYK3_ARATH (F21J9.27 OS=Arabidopsis thaliana GN=At1g24610 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.080e-7
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3 
Query:   12 MLMSSSLSHVRPLTCASSS---RLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            M ++S  + +RP TC ++S   RL  HP DL++W+KREGGFVH AVK++Q
Sbjct:    6 MAIASLAAQIRPFTCLAASLPSRLAPHPPDLIRWIKREGGFVHHAVKLSQ 55
[Close]
BLAST of AM161962 vs. TrEMBL
Match: Q8L9I6_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.080e-7
Identity = 26/50 (52.00%), Postives = 37/50 (74.00%), Query Frame = 3 
Query:   12 MLMSSSLSHVRPLTCASSS---RLVSHPHDLVKWVKREGGFVHRAVKIAQ 152
            M ++S  + +RP TC ++S   RL  HP DL++W+KREGGFVH AVK++Q
Sbjct:    1 MAIASLAAQIRPFTCLAASLPSRLAPHPPDLIRWIKREGGFVHHAVKLSQ 50
[Close]
BLAST of AM161962 vs. TrEMBL
Match: D7T8L6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011666001 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.410e-7
Identity = 37/80 (46.25%), Postives = 47/80 (58.75%), Query Frame = 3 
Query:    6 AAMLMSSSLSHVRPLTCASSS---------RLVSHPHDLVKWVKREGGFVHRAVKIA-QPDSXXXXXXXXXVAKEEIPIG 215
            A +  S  L+  RPLTCA+++         RLV HP DL+KWV+REGGFVH+AV IA   DS         VA ++IP G
Sbjct:    2 AGLGASKILTQFRPLTCAAATATATACHPGRLVPHPPDLIKWVRREGGFVHQAVTIAPSADS----CGLGLVASQDIPKG 77
[Close]
BLAST of AM161962 vs. Lotus protein
Match: chr4.CM0042.1220.r2.m (- phase: 0 )

HSP 1 Score: 81.2629 bits (199), Expect = 9.451e-17
Identity = 43/68 (63.24%), Postives = 52/68 (76.47%), Query Frame = 3 
Query:   15 LMSSSLSHVRPLTCASS-SRLVSHPHDLVKWVKREGGFVHRAVKIAQPDSXXXXXXXXXVAKEEIPIG 215
            ++ SSL+H+RPLTCA+S SR+VSHP DL+ WV REGGFVHRAVKIAQ  S         VAK+EIP+G
Sbjct:    6 MVMSSLTHLRPLTCAASASRIVSHPPDLINWVTREGGFVHRAVKIAQLGS----NGLGLVAKQEIPMG 69
[Close]
BLAST of AM161962 vs. Soybean peptides
Match: Glyma08g05020.1|PACid:16270013 ()

HSP 1 Score: 75.0998 bits (183), Expect = 1.561e-14
Identity = 43/65 (66.15%), Postives = 46/65 (70.77%), Query Frame = 3 
Query:   27 SLSHVRPLTCASSS--RLVSHPHDLVKWVKREGGFVHRAVKIAQPDSXXXXXXXXXVAKEEIPIG 215
            SLS +RPLTCAS+S  RLV  P DL+KWV REGGFVHRAVKI Q DS         VAKEEIP G
Sbjct:   10 SLSQLRPLTCASTSASRLVPQPPDLIKWVTREGGFVHRAVKITQLDS---SNGLGLVAKEEIPRG 71
[Close]
BLAST of AM161962 vs. Soybean peptides
Match: Glyma05g34650.1|PACid:16260694 ()

HSP 1 Score: 63.929 bits (154), Expect = 3.598e-11
Identity = 37/63 (58.73%), Postives = 42/63 (66.67%), Query Frame = 3 
Query:   27 SLSHVRPLTCASSSRLVSHPHDLVKWVKREGGFVHRAVKIAQPDSXXXXXXXXXVAKEEIPIG 215
            SL+ +RPLTCA+S+       DL+KWV REGGFVHRAVKIAQ DS         V KEEIP G
Sbjct:    3 SLTQLRPLTCAASAP------DLIKWVAREGGFVHRAVKIAQLDS---SNGLGLVTKEEIPRG 56
[Close]
BLAST of AM161962 vs. Medicago proteins
Match: IMGA|Medtr8g105200.1 (SET domain-containing protein (AHRD V1 ***- B9HJF9_POPTR); contains Interpro domain(s) IPR001214 SET chr08_pseudomolecule_IMGAG_V3.5 31185978-31182186 E EGN_Mt100125 20100825)

HSP 1 Score: 98.2117 bits (243), Expect = 1.058e-21
Identity = 53/75 (70.67%), Postives = 57/75 (76.00%), Query Frame = 3 
Query:    3 KAAMLMSSSLSHVRPLTCASS----SRLVSHPHDLVKWVKREGGFVHRAVKIAQPDSXXXXXXXXXVAKEEIPIG 215
            K AMLMSSSLSHVRPLTC+SS    SR+  HPHDL+KWV REGGFVHRAVKIAQ DS         VAKE+IPIG
Sbjct:   10 KTAMLMSSSLSHVRPLTCSSSTSSSSRIAPHPHDLIKWVTREGGFVHRAVKIAQLDS---SNGLGLVAKEDIPIG 81
The following BLAST results are available for this feature:
BLAST of AM161962 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 6
Match NameE-valueIdentityDescription
B9SJ24_RICCO8.342e-1166.00Putative uncharacterized protein OS=Ricinus commun... [more]
D7KAL1_ARALY3.875e-852.00Putative uncharacterized protein OS=Arabidopsis ly... [more]
B9HJG0_POPTR5.061e-845.07Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q9FYK3_ARATH8.633e-852.00F21J9.27 OS=Arabidopsis thaliana GN=At1g24610 PE=4... [more]
Q8L9I6_ARATH8.633e-852.00Putative uncharacterized protein OS=Arabidopsis th... [more]
D7T8L6_VITVI1.127e-746.25Whole genome shotgun sequence of line PN40024, sca... [more]
back to top
BLAST of AM161962 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT1G24610.13.316e-1052.00| Symbols: | Rubisco methyltransferase family pro... [more]
back to top
BLAST of AM161962 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT1G24610.13.316e-1052.00| Symbols: | Rubisco methyltransferase family pro... [more]
back to top
BLAST of AM161962 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 6
Match NameE-valueIdentityDescription
B9SJ24_RICCO1.043e-1066.00Putative uncharacterized protein OS=Ricinus commun... [more]
D7KAL1_ARALY4.846e-852.00Putative uncharacterized protein OS=Arabidopsis ly... [more]
B9HJG0_POPTR6.329e-845.07Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q9FYK3_ARATH1.080e-752.00F21J9.27 OS=Arabidopsis thaliana GN=At1g24610 PE=4... [more]
Q8L9I6_ARATH1.080e-752.00Putative uncharacterized protein OS=Arabidopsis th... [more]
D7T8L6_VITVI1.410e-746.25Whole genome shotgun sequence of line PN40024, sca... [more]
back to top
BLAST of AM161962 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 1
Match NameE-valueIdentityDescription
chr4.CM0042.1220.r2.m9.451e-1763.24- phase: 0 [more]
back to top
BLAST of AM161962 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 2
Match NameE-valueIdentityDescription
Glyma08g05020.1|PACid:162700131.561e-1466.15[more]
Glyma05g34650.1|PACid:162606943.598e-1158.73[more]
back to top
BLAST of AM161962 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 1
Match NameE-valueIdentityDescription
IMGA|Medtr8g105200.11.058e-2170.67SET domain-containing protein (AHRD V1 ***- B9HJF9... [more]
back to top
InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 52..62
score
NoneNo IPR availableSEGsegsegcoord: 54..62
score
NoneNo IPR availableSEGsegsegcoord: 2..8
score: NAcoord: 54..64
score

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 52..62
score
NoneNo IPR availableSEGsegsegcoord: 54..62
score
NoneNo IPR availableSEGsegsegcoord: 2..8
score: NAcoord: 54..64
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v2
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM161962 ID=AM161962; Name=AM161962; organism=Pisum sativum; type=EST; length=215bp
TGAAGGCGGCGATGTTAATGTCGTCGTCGTTGAGTCACGTGCGTCCTCTA
ACATGCGCTTCGTCGTCGCGATTAGTGTCGCATCCACACGACCTTGTGAA
ATGGGTAAAAAGAGAAGGTGGTTTCGTTCACCGTGCGGTGAAGATAGCGC
AACCCGATTCCGGTGGTTTAGGGTTAGGGTTAGGGTTAGTGGCTAAAGAA
GAAATTCCGATTGGT
back to top