AM162167

EST Overview


Name	AM162167
Unique Name	AM162167
Type	EST
Organism	Pisum sativum (pea)
Sequence length	480

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM162167 vs. TrEMBL
Match: A5BEM6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021914 PE=3 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 6.034e-65
Identity = 77/83 (92.77%), Postives = 80/83 (96.39%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFTGKEKIIKF
Sbjct:  104 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENILAEMVISAVPSIEMVRFVNSGTEACMGMLRLARAFTGKEKIIKF 186

HSP 2 Score: 114.775 bits (286), Expect = 6.034e-65
Identity = 58/74 (78.38%), Postives = 62/74 (83.78%), Query Frame = 1

Query:    7 SLTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            S+ MAVS D+K K+    TLRKSEEAF  AKELMPGGVNSPVRAFKSVGGQPI+ DSVKGS MWDID NEYIDY
Sbjct:   33 SVKMAVSVDEKKKS---FTLRKSEEAFEIAKELMPGGVNSPVRAFKSVGGQPILFDSVKGSHMWDIDGNEYIDY 103

[Close]

BLAST of AM162167 vs. TrEMBL
Match: D7SH28_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00008589001 PE=4 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.747e-64
Identity = 77/83 (92.77%), Postives = 80/83 (96.39%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFTGKEKIIKF
Sbjct:   69 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENILAEMVISAVPSIEMVRFVNSGTEACMGMLRLARAFTGKEKIIKF 151

HSP 2 Score: 113.235 bits (282), Expect = 1.747e-64
Identity = 57/71 (80.28%), Postives = 60/71 (84.51%), Query Frame = 1

Query:   16 MAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            MAVS D+K K+    TLRKSEEAF  AKELMPGGVNSPVRAFKSVGGQPI+ DSVKGS MWDID NEYIDY
Sbjct:    1 MAVSVDEKKKS---FTLRKSEEAFEIAKELMPGGVNSPVRAFKSVGGQPILFDSVKGSHMWDIDGNEYIDY 68

[Close]

BLAST of AM162167 vs. TrEMBL
Match: Q84TK5_BRANA (Glutamate 1-semialdehyde aminotransferase OS=Brassica napus GN=Gsa PE=2 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 5.044e-64
Identity = 75/83 (90.36%), Postives = 78/83 (93.98%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFT KEK IKF
Sbjct:  104 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGVLRLARAFTNKEKFIKF 186

HSP 2 Score: 114.775 bits (286), Expect = 5.044e-64
Identity = 56/74 (75.68%), Postives = 65/74 (87.84%), Query Frame = 1

Query:    7 SLTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            S+ M+VS D+K K+    TL+KSEEAF AAK+LMPGGVNSPVRAFKSVGGQP++IDSVKGS+MWDID NEYIDY
Sbjct:   33 SIKMSVSVDEKKKS---FTLQKSEEAFNAAKDLMPGGVNSPVRAFKSVGGQPVLIDSVKGSKMWDIDGNEYIDY 103

[Close]

BLAST of AM162167 vs. TrEMBL
Match: D7MPQ2_ARALY (Glutamate-1-semialdehyde-2,1-aminomutase OS=Arabidopsis lyrata subsp. lyrata GN=GSA1 PE=4 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 2.480e-63
Identity = 75/83 (90.36%), Postives = 78/83 (93.98%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFT KEK IKF
Sbjct:  105 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGVLRLARAFTNKEKFIKF 187

HSP 2 Score: 112.464 bits (280), Expect = 2.480e-63
Identity = 55/73 (75.34%), Postives = 63/73 (86.30%), Query Frame = 1

Query:   10 LTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            + M+VS D+K K+    TL+KSEEAF AAK LMPGGVNSPVRAFKSVGGQP++IDSVKGS+MWDID NEYIDY
Sbjct:   35 IKMSVSVDEKKKS---FTLQKSEEAFNAAKNLMPGGVNSPVRAFKSVGGQPVLIDSVKGSKMWDIDGNEYIDY 104

[Close]

BLAST of AM162167 vs. TrEMBL
Match: D7LS04_ARALY (Glutamate-1-semialdehyde 2,1-aminomutase 2 OS=Arabidopsis lyrata subsp. lyrata GN=GSA2 PE=4 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 5.990e-62
Identity = 75/83 (90.36%), Postives = 79/83 (95.18%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFTGK+K IKF
Sbjct:  107 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGVLRLARAFTGKQKFIKF 189

HSP 2 Score: 106.686 bits (265), Expect = 5.990e-62
Identity = 53/75 (70.67%), Postives = 63/75 (84.00%), Query Frame = 1

Query:    4 RSLTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            R + M+VS ++K   T+K TL+KSEEAF AAK LMPGGVNSPVRAFKSVGGQP+++DS KGSR+ DID NEYIDY
Sbjct:   35 RCIKMSVSVEEK---TKKFTLQKSEEAFNAAKNLMPGGVNSPVRAFKSVGGQPVVMDSAKGSRIRDIDGNEYIDY 106

[Close]

BLAST of AM162167 vs. TrEMBL
Match: B9IFG3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_575404 PE=3 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 1.018e-61
Identity = 75/83 (90.36%), Postives = 79/83 (95.18%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI AVPSIEMVRFVNSGTEACMG LRLARA+TG+EKIIKF
Sbjct:  110 VGSWGPAIIGHADDEVLAALSETMKKGTSFGAPCLLENVLAEMVIKAVPSIEMVRFVNSGTEACMGVLRLARAYTGREKIIKF 192

HSP 2 Score: 106.301 bits (264), Expect = 1.018e-61
Identity = 53/74 (71.62%), Postives = 62/74 (83.78%), Query Frame = 1

Query:    7 SLTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            S+ MAVS +++ K  +  TL KSEEAF+AAKE+MPGGVNSPVRAFKSVGGQPI+ DSVKGS M DID N+YIDY
Sbjct:   37 SIKMAVSVEEEKKK-KSFTLEKSEEAFSAAKEMMPGGVNSPVRAFKSVGGQPIVFDSVKGSHMRDIDGNDYIDY 109

[Close]

BLAST of AM162167 vs. TrEMBL
Match: B7ZYW4_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 7.107e-60
Identity = 74/83 (89.16%), Postives = 79/83 (95.18%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+V AAL ET+KKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMGALRL RAFTG+EKIIKF
Sbjct:  105 VGSWGPAIIGHADDKVNAALIETLKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGALRLVRAFTGREKIIKF 187

HSP 2 Score: 103.99 bits (258), Expect = 7.107e-60
Identity = 48/62 (77.42%), Postives = 54/62 (87.10%), Query Frame = 1

Query:   43 KTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            K  +  T++KSEE F AAKELMPGGVNSPVRAFKSVGGQP++ DSVKGSRMWD+D NEYIDY
Sbjct:   43 KGEKAYTVQKSEEIFNAAKELMPGGVNSPVRAFKSVGGQPVVFDSVKGSRMWDVDGNEYIDY 104

[Close]

BLAST of AM162167 vs. TrEMBL
Match: B6TPE4_MAIZE (Glutamate-1-semialdehyde 2,1-aminomutase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 7.107e-60
Identity = 74/83 (89.16%), Postives = 79/83 (95.18%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+V AAL ET+KKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMGALRL RAFTG+EKIIKF
Sbjct:  104 VGSWGPAIIGHADDKVNAALIETLKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGALRLVRAFTGREKIIKF 186

HSP 2 Score: 103.99 bits (258), Expect = 7.107e-60
Identity = 48/62 (77.42%), Postives = 54/62 (87.10%), Query Frame = 1

Query:   43 KTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            K  +  T++KSEE F AAKELMPGGVNSPVRAFKSVGGQP++ DSVKGSRMWD+D NEYIDY
Sbjct:   42 KGEKAYTVQKSEEIFNAAKELMPGGVNSPVRAFKSVGGQPVVFDSVKGSRMWDVDGNEYIDY 103

[Close]

BLAST of AM162167 vs. TrEMBL
Match: Q0J464_ORYSJ (Os08g0532200 protein OS=Oryza sativa subsp. japonica GN=Os08g0532200 PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 4.552e-59
Identity = 72/83 (86.75%), Postives = 78/83 (93.98%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD V AAL ET+KKGTSFGAPC+LEN LAEMVI+AVPSIEMVRFVNSGTEACMGALRL RAFTG+EKI+KF
Sbjct:  109 VGSWGPAIIGHADDTVNAALIETLKKGTSFGAPCVLENVLAEMVISAVPSIEMVRFVNSGTEACMGALRLVRAFTGREKILKF 191

HSP 2 Score: 103.99 bits (258), Expect = 4.552e-59
Identity = 49/62 (79.03%), Postives = 53/62 (85.48%), Query Frame = 1

Query:   43 KTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            K  +  T+ KSEE F AAKELMPGGVNSPVRAFKSVGGQPI+ DSVKGSRMWD+D NEYIDY
Sbjct:   47 KGEKAYTVEKSEEIFNAAKELMPGGVNSPVRAFKSVGGQPIVFDSVKGSRMWDVDGNEYIDY 108

[Close]

BLAST of AM162167 vs. TrEMBL
Match: A9S7G9_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_181992 PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 5.326e-54
Identity = 63/83 (75.90%), Postives = 73/83 (87.95%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAI+G  D+QV   L  T++KGTSFGAPC+LEN LA+MVI AVPSIEMVRFVNSGTEACMG LRLARA+TG+EKI+KF
Sbjct:  116 VGSWGPAIVGACDEQVNEELIRTLQKGTSFGAPCVLENVLAQMVIDAVPSIEMVRFVNSGTEACMGMLRLARAYTGREKILKF 198

HSP 2 Score: 103.219 bits (256), Expect = 5.326e-54
Identity = 50/75 (66.67%), Postives = 59/75 (78.67%), Query Frame = 1

Query:    4 RSLTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            R + M +S ++K  TT     +KSEE FAAAKELMPGGVNSPVRAFKSVGGQPI+ D VKGS+ WDID N+Y+DY
Sbjct:   46 RVVQMTISVEKKQFTT-----KKSEETFAAAKELMPGGVNSPVRAFKSVGGQPIVFDRVKGSKAWDIDGNQYVDY 115

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA_BRANA (Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic OS=Brassica napus GN=GSA PE=2 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 6.058e-65
Identity = 75/83 (90.36%), Postives = 78/83 (93.98%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFT KEK IKF
Sbjct:  104 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGVLRLARAFTNKEKFIKF 186

HSP 2 Score: 114.005 bits (284), Expect = 6.058e-65
Identity = 56/74 (75.68%), Postives = 64/74 (86.49%), Query Frame = 1

Query:    7 SLTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            S+ M+VS D+K K+    TL+KSEEAF AAK LMPGGVNSPVRAFKSVGGQP++IDSVKGS+MWDID NEYIDY
Sbjct:   33 SIKMSVSVDEKKKS---FTLQKSEEAFNAAKNLMPGGVNSPVRAFKSVGGQPVLIDSVKGSKMWDIDGNEYIDY 103

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA1_ARATH (Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic OS=Arabidopsis thaliana GN=GSA1 PE=1 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 5.063e-64
Identity = 75/83 (90.36%), Postives = 78/83 (93.98%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFT KEK IKF
Sbjct:  105 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGVLRLARAFTNKEKFIKF 187

HSP 2 Score: 110.923 bits (276), Expect = 5.063e-64
Identity = 54/73 (73.97%), Postives = 63/73 (86.30%), Query Frame = 1

Query:   10 LTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            + M+VS D+K K+    +L+KSEEAF AAK LMPGGVNSPVRAFKSVGGQP++IDSVKGS+MWDID NEYIDY
Sbjct:   35 IKMSVSVDEKKKS---FSLQKSEEAFNAAKNLMPGGVNSPVRAFKSVGGQPVLIDSVKGSKMWDIDGNEYIDY 104

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA2_ARATH (Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic OS=Arabidopsis thaliana GN=GSA2 PE=2 SV=2)

HSP 1 Score: 155.221 bits (391), Expect = 4.231e-63
Identity = 75/83 (90.36%), Postives = 79/83 (95.18%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFTGK+K IKF
Sbjct:  103 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGVLRLARAFTGKQKFIKF 185

HSP 2 Score: 106.686 bits (265), Expect = 4.231e-63
Identity = 53/75 (70.67%), Postives = 63/75 (84.00%), Query Frame = 1

Query:    4 RSLTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            R + M+VS ++K   T+K TL+KSEEAF AAK LMPGGVNSPVRAFKSVGGQP+++DS KGSR+ DID NEYIDY
Sbjct:   31 RCIKMSVSVEEK---TKKFTLQKSEEAFNAAKNLMPGGVNSPVRAFKSVGGQPVVMDSAKGSRIRDIDGNEYIDY 102

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA_ORYSJ (Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic OS=Oryza sativa subsp. japonica GN=GSA PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 3.215e-60
Identity = 72/83 (86.75%), Postives = 78/83 (93.98%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD V AAL ET+KKGTSFGAPC+LEN LAEMVI+AVPSIEMVRFVNSGTEACMGALRL RAFTG+EKI+KF
Sbjct:  109 VGSWGPAIIGHADDTVNAALIETLKKGTSFGAPCVLENVLAEMVISAVPSIEMVRFVNSGTEACMGALRLVRAFTGREKILKF 191

HSP 2 Score: 103.99 bits (258), Expect = 3.215e-60
Identity = 49/62 (79.03%), Postives = 53/62 (85.48%), Query Frame = 1

Query:   43 KTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            K  +  T+ KSEE F AAKELMPGGVNSPVRAFKSVGGQPI+ DSVKGSRMWD+D NEYIDY
Sbjct:   47 KGEKAYTVEKSEEIFNAAKELMPGGVNSPVRAFKSVGGQPIVFDSVKGSRMWDVDGNEYIDY 108

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA_HORVU (Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic OS=Hordeum vulgare GN=GSA PE=1 SV=3)

HSP 1 Score: 147.132 bits (370), Expect = 5.466e-60
Identity = 71/83 (85.54%), Postives = 78/83 (93.98%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+V AAL ET+KKGTSFGAPC LEN LA+MVI+AVPSIEMVRFVNSGTEACMGALRL RAFTG+EKI+KF
Sbjct:  100 VGSWGPAIIGHADDKVNAALIETLKKGTSFGAPCALENVLAQMVISAVPSIEMVRFVNSGTEACMGALRLVRAFTGREKILKF 182

HSP 2 Score: 104.375 bits (259), Expect = 5.466e-60
Identity = 52/70 (74.29%), Postives = 57/70 (81.43%), Query Frame = 1

Query:   19 AVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            AVS D+K       T++KSEE F AAKELMPGGVNSPVRAFKSVGGQPI+ DSVKGS MWD+D NEYIDY
Sbjct:   35 AVSIDEKA-----YTVQKSEEIFNAAKELMPGGVNSPVRAFKSVGGQPIVFDSVKGSHMWDVDGNEYIDY 99

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA_SYNP2 (Glutamate-1-semialdehyde 2,1-aminomutase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=hemL PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 1.417e-54
Identity = 67/83 (80.72%), Postives = 74/83 (89.16%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +G+WGPAI GHA+D+V AAL ET++KGTSFGAPCL EN LAEMVI AVPSIEMVRFVNSGTEACM  LRL RAFTG+EKIIKF
Sbjct:   63 VGTWGPAICGHANDEVNAALRETLEKGTSFGAPCLKENILAEMVINAVPSIEMVRFVNSGTEACMSVLRLMRAFTGREKIIKF 145

HSP 2 Score: 95.9005 bits (237), Expect = 1.417e-54
Identity = 44/60 (73.33%), Postives = 51/60 (85.00%), Query Frame = 1

Query:   49 TEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            T  L   KSEE F+AA++LMPGGV+SPVRAFKSVGGQPI+ D VKGSR+WD+D NEYIDY
Sbjct:    3 TTALNTTKSEEIFSAAQKLMPGGVSSPVRAFKSVGGQPIVFDRVKGSRVWDVDGNEYIDY 62

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA_ACAM1 (Glutamate-1-semialdehyde 2,1-aminomutase OS=Acaryochloris marina (strain MBIC 11017) GN=hemL PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 1.285e-52
Identity = 63/83 (75.90%), Postives = 71/83 (85.54%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +G+WGPAI GHA  +VL AL E + KGTSFGAPCLLEN LAEMVI AVPS+E+VRFVNSGTEACM  LRL RAFTG+EK+IKF
Sbjct:   63 VGTWGPAICGHAHPEVLTALKEALDKGTSFGAPCLLENVLAEMVIDAVPSVEVVRFVNSGTEACMSVLRLMRAFTGREKVIKF 145

HSP 2 Score: 93.9745 bits (232), Expect = 1.285e-52
Identity = 42/60 (70.00%), Postives = 51/60 (85.00%), Query Frame = 1

Query:   49 TEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            T+ L   KSEE FAAA++LMPGGV+SPVRAFKSVGGQPI+ D VKG+ +WD+D N+YIDY
Sbjct:    3 TDTLNTTKSEEIFAAAQKLMPGGVSSPVRAFKSVGGQPIVFDRVKGAHVWDVDGNQYIDY 62

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA_CYAA5 (Glutamate-1-semialdehyde 2,1-aminomutase OS=Cyanothece sp. (strain ATCC 51142) GN=hemL PE=3 SV=2)

HSP 1 Score: 132.494 bits (332), Expect = 1.071e-51
Identity = 63/83 (75.90%), Postives = 72/83 (86.75%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +G+WGPAI GHA  +V+AAL + + +GTSFGAPCLLEN LAEMVI AVPSIEMVRFVNSGTEACM  LRL RAFTG++KIIKF
Sbjct:   64 VGTWGPAICGHAHPEVIAALHDALDRGTSFGAPCLLENVLAEMVIDAVPSIEMVRFVNSGTEACMSVLRLMRAFTGRDKIIKF 146

HSP 2 Score: 91.2781 bits (225), Expect = 1.071e-51
Identity = 41/61 (67.21%), Postives = 51/61 (83.61%), Query Frame = 1

Query:   46 TTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            +T  L   KSE+ FAAA++LMPGGV+SPVRAFKSVGGQPI+ D VKG+ +WD+D N+YIDY
Sbjct:    3 STTSLKTTKSEDIFAAAQKLMPGGVSSPVRAFKSVGGQPIVFDHVKGAYIWDVDGNQYIDY 63

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA_PROM0 (Glutamate-1-semialdehyde 2,1-aminomutase OS=Prochlorococcus marinus (strain MIT 9301) GN=hemL PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 1.396e-51
Identity = 63/83 (75.90%), Postives = 72/83 (86.75%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            IGSWGPAI GHA  +V+ AL E ++KGTSFGAPC+LEN LAEMVI AVPS+EMVRFVNSGTEACM  LRL RAFTG++K+IKF
Sbjct:   62 IGSWGPAICGHAHPEVITALHEAIEKGTSFGAPCVLENKLAEMVIDAVPSVEMVRFVNSGTEACMAVLRLMRAFTGRDKVIKF 144

HSP 2 Score: 90.5077 bits (223), Expect = 1.396e-51
Identity = 43/60 (71.67%), Postives = 48/60 (80.00%), Query Frame = 1

Query:   49 TEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            T+ L   KSEE F+AA+ELMPGGV+SPVRAFKSVGGQPI+ D VKG   WDID N YIDY
Sbjct:    2 TDILNYTKSEEIFSAAQELMPGGVSSPVRAFKSVGGQPIVFDRVKGPFAWDIDGNRYIDY 61

[Close]

BLAST of AM162167 vs. SwissProt
Match: GSA_THEEB (Glutamate-1-semialdehyde 2,1-aminomutase OS=Thermosynechococcus elongatus (strain BP-1) GN=hemL PE=1 SV=2)

HSP 1 Score: 131.724 bits (330), Expect = 1.819e-51
Identity = 62/83 (74.70%), Postives = 72/83 (86.75%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAI+GHA  +V+ AL   ++KGTSFGAPCLLEN LAEMVIAAVPS+EMVRFVNSGTEACM  LRL RA+T +EK+IKF
Sbjct:   68 VGSWGPAIVGHAHPEVIDALHAALEKGTSFGAPCLLENILAEMVIAAVPSVEMVRFVNSGTEACMAVLRLMRAYTQREKVIKF 150

HSP 2 Score: 91.2781 bits (225), Expect = 1.819e-51
Identity = 41/61 (67.21%), Postives = 50/61 (81.97%), Query Frame = 1

Query:   46 TTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            TT      KS+E FAAA++LMPGGV+SPVRAFKSVGGQPI+ D VKG+ +WD+D N+YIDY
Sbjct:    7 TTTVFQTTKSQEIFAAAQKLMPGGVSSPVRAFKSVGGQPIVFDHVKGAHIWDVDGNQYIDY 67

[Close]

BLAST of AM162167 vs. TAIR peptide
Match: AT5G63570.1 (| Symbols: GSA1 | glutamate-1-semialdehyde-2,1-aminomutase | chr5:25451957-25453620 FORWARD LENGTH=474)

HSP 1 Score: 154.066 bits (388), Expect = 5.860e-65
Identity = 75/83 (90.36%), Postives = 78/83 (93.98%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFT KEK IKF
Sbjct:  105 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGVLRLARAFTNKEKFIKF 187

HSP 2 Score: 110.923 bits (276), Expect = 5.860e-65
Identity = 54/73 (73.97%), Postives = 63/73 (86.30%), Query Frame = 1

Query:   10 LTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            + M+VS D+K K+    +L+KSEEAF AAK LMPGGVNSPVRAFKSVGGQP++IDSVKGS+MWDID NEYIDY
Sbjct:   35 IKMSVSVDEKKKS---FSLQKSEEAFNAAKNLMPGGVNSPVRAFKSVGGQPVLIDSVKGSKMWDIDGNEYIDY 104

[Close]

BLAST of AM162167 vs. TAIR peptide
Match: AT3G48730.1 (| Symbols: GSA2 | glutamate-1-semialdehyde 2,1-aminomutase 2 | chr3:18049697-18051550 FORWARD LENGTH=472)

HSP 1 Score: 155.221 bits (391), Expect = 4.897e-64
Identity = 75/83 (90.36%), Postives = 79/83 (95.18%), Query Frame = 2

Query:  230 IGSWGPAIIGHADDQVLAALGETMKKGTSFGAPCLLENTLAEMVIAAVPSIEMVRFVNSGTEACMGALRLARAFTGKEKIIKF 478
            +GSWGPAIIGHADD+VLAAL ETMKKGTSFGAPCLLEN LAEMVI+AVPSIEMVRFVNSGTEACMG LRLARAFTGK+K IKF
Sbjct:  103 VGSWGPAIIGHADDEVLAALAETMKKGTSFGAPCLLENVLAEMVISAVPSIEMVRFVNSGTEACMGVLRLARAFTGKQKFIKF 185

HSP 2 Score: 106.686 bits (265), Expect = 4.897e-64
Identity = 53/75 (70.67%), Postives = 63/75 (84.00%), Query Frame = 1

Query:    4 RSLTMAVSTDQKPKTTEKLTLRKSEEAFAAAKELMPGGVNSPVRAFKSVGGQPIIIDSVKGSRMWDIDDNEYIDY 228
            R + M+VS ++K   T+K TL+KSEEAF AAK LMPGGVNSPVRAFKSVGGQP+++DS KGSR+ DID NEYIDY
Sbjct:   31 RCIKMSVSVEEK---TKKFTLQKSEEAFNAAKNLMPGGVNSPVRAFKSVGGQPVVMDSAKGSRIRDIDGNEYIDY 102

The following BLAST results are available for this feature:

BLAST of AM162167 vs. TrEMBL
BLAST of AM162167 vs. SwissProt
BLAST of AM162167 vs. TAIR peptide

BLAST of AM162167 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A5BEM6_VITVI	6.034e-65	92.77	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7SH28_VITVI	1.747e-64	92.77	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q84TK5_BRANA	5.044e-64	90.36	Glutamate 1-semialdehyde aminotransferase OS=Brass...	[more]
D7MPQ2_ARALY	2.480e-63	90.36	Glutamate-1-semialdehyde-2,1-aminomutase OS=Arabid...	[more]
D7LS04_ARALY	5.990e-62	90.36	Glutamate-1-semialdehyde 2,1-aminomutase 2 OS=Arab...	[more]
B9IFG3_POPTR	1.018e-61	90.36	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B7ZYW4_MAIZE	7.107e-60	89.16	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6TPE4_MAIZE	7.107e-60	89.16	Glutamate-1-semialdehyde 2,1-aminomutase OS=Zea ma...	[more]
Q0J464_ORYSJ	4.552e-59	86.75	Os08g0532200 protein OS=Oryza sativa subsp. japoni...	[more]
A9S7G9_PHYPA	5.326e-54	75.90	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of AM162167 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GSA_BRANA	6.058e-65	90.36	Glutamate-1-semialdehyde 2,1-aminomutase, chloropl...	[more]
GSA1_ARATH	5.063e-64	90.36	Glutamate-1-semialdehyde 2,1-aminomutase 1, chloro...	[more]
GSA2_ARATH	4.231e-63	90.36	Glutamate-1-semialdehyde 2,1-aminomutase 2, chloro...	[more]
GSA_ORYSJ	3.215e-60	86.75	Glutamate-1-semialdehyde 2,1-aminomutase, chloropl...	[more]
GSA_HORVU	5.466e-60	85.54	Glutamate-1-semialdehyde 2,1-aminomutase, chloropl...	[more]
GSA_SYNP2	1.417e-54	80.72	Glutamate-1-semialdehyde 2,1-aminomutase OS=Synech...	[more]
GSA_ACAM1	1.285e-52	75.90	Glutamate-1-semialdehyde 2,1-aminomutase OS=Acaryo...	[more]
GSA_CYAA5	1.071e-51	75.90	Glutamate-1-semialdehyde 2,1-aminomutase OS=Cyanot...	[more]
GSA_PROM0	1.396e-51	75.90	Glutamate-1-semialdehyde 2,1-aminomutase OS=Prochl...	[more]
GSA_THEEB	1.819e-51	74.70	Glutamate-1-semialdehyde 2,1-aminomutase OS=Thermo...	[more]

BLAST of AM162167 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G63570.1	5.860e-65	90.36	\| Symbols: GSA1 \| glutamate-1-semialdehyde-2,1-ami...	[more]
AT3G48730.1	4.897e-64	90.36	\| Symbols: GSA2 \| glutamate-1-semialdehyde 2,1-ami...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005814	Aminotransferase class-III	PANTHER	PTHR11986	AMINOTRANSFERASE CLASS III	coord: 42..76 score: 1.4
IPR005814	Aminotransferase class-III	PANTHER	PTHR11986	AMINOTRANSFERASE CLASS III	coord: 42..124 score: 7.8
IPR005814	Aminotransferase class-III	PFAM	PF00202	Aminotran_3	coord: 39..124 score: 3.2
IPR015422	Pyridoxal phosphate-dependent transferase, major region, subdomain 2	GENE3D	3.90.1150.10	no description	coord: 22..76 score: 1.4
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 20..85 score: 9.4
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 42..124 score: 8.6
None	No IPR available	PANTHER	PTHR11986:SF5	GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE	coord: 42..76 score: 1.4
None	No IPR available	PANTHER	PTHR11986:SF5	GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE	coord: 42..124 score: 7.8
IPR015421	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	GENE3D	3.40.640.10	no description	coord: 56..124 score: 5.2

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162167 ID=AM162167; Name=AM162167; organism=Pisum sativum; type=EST; length=480bp
ACTCGTTCTCTAACTATGGCTGTTTCCACCGACCAGAAACCCAAAACCAC
CGAAAAACTCACTCTCCGCAAGTCCGAAGAAGCTTTCGCCGCTGCAAAGG
AATTGATGCCTGGAGGAGTTAACTCTCCCGTTCGTGCGTTCAAATCAGTT
GGTGGACAACCCATTATCATTGACTCAGTTAAAGGCTCTCGGATGTGGGA
CATTGATGACAACGAATATATTGACTACGATTGGTTCTTGGGGTCCTGCA
ATTATCGGTCACGCCGATGATCAGGTTCTTGCTGCTCTGGGGGAAACAAT
GAAGAAAGGAACCAGTTTTGGTGCACCTTGTCTATTGGAGAACACGTTAG
CAGAGATGGTTATCGCTGCTGTTCCCAGCATTGAAATGGTGAGATTTGTG
AATTCGGGAACGGAAGCGTGCATGGGTGCACTCCGTCTGGCGCGTGCTTT
TACTGGGAAGGAGAAGATCATCAAGTTTGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008483	transaminase activity
GO:0030170	pyridoxal phosphate binding
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR005814	Aminotrans_3
IPR015422	PyrdxlP-dep_Trfase_small
IPR015424	PyrdxlP-dep_Trfase
IPR015421	PyrdxlP-dep_Trfase_major

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