CD860330

EST Overview


Name	CD860330
Unique Name	CD860330
Type	EST
Organism	Pisum sativum (pea)
Sequence length	413

Libraries


Library Name	Type
LIBEST_014003 TE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD860330 vs. TrEMBL
Match: C6TK07_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 1.329e-61
Identity = 113/137 (82.48%), Postives = 125/137 (91.24%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LLAKPQTYMNFSGESVGPLAA+Y+VPLRHILLV+DETSLPNGVL+LQPK GHGHHNGLKNV+GHLDG   FPRLA+GIGNPPGTMDLRA+LLQKFS  ERKQID SLE  V AVRT+ +NGF+ HVNRFNLGQKY+
Sbjct:  104 ILLAKPQTYMNFSGESVGPLAAYYQVPLRHILLVFDETSLPNGVLRLQPKGGHGHHNGLKNVMGHLDGCSAFPRLAVGIGNPPGTMDLRAYLLQKFSVEERKQIDASLEQAVAAVRTVVLNGFDQHVNRFNLGQKYK 240

[Close]

BLAST of CD860330 vs. TrEMBL
Match: B9RSL9_RICCO (Peptidyl-tRNA hydrolase, putative OS=Ricinus communis GN=RCOM_1726890 PE=3 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 8.912e-58
Identity = 103/137 (75.18%), Postives = 121/137 (88.32%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LL KPQ YMNFSGESVGPLAAHY+VPLRHILL+YDE SLPNGV++LQPK GHGHHNG+K+V+ HLDG  +FPRL IGIGNPPGTMD+RAFLLQKFSS ER+QID +L  G+ AVRTL ++GFN ++ RFNLGQKY+
Sbjct:  113 ILLVKPQAYMNFSGESVGPLAAHYQVPLRHILLIYDEMSLPNGVMRLQPKGGHGHHNGVKSVMEHLDGCREFPRLCIGIGNPPGTMDMRAFLLQKFSSTEREQIDAALGQGIDAVRTLVLSGFNQNITRFNLGQKYK 249

[Close]

BLAST of CD860330 vs. TrEMBL
Match: B9H311_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_648034 PE=3 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 9.854e-57
Identity = 100/137 (72.99%), Postives = 120/137 (87.59%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LLAKPQ YMNFSGESVGPLAAHY++PLRHILL+YDE +LPNG+L++QPK GHGHHNG+K+V+ HLDG  +FPRL IGIGNPPGTMD++AFLLQKFS  ER+QID SLE GV A+RTL + GF + + RFNLGQKY+
Sbjct:  104 ILLAKPQAYMNFSGESVGPLAAHYKIPLRHILLIYDEMNLPNGILRVQPKGGHGHHNGVKSVMDHLDGCREFPRLCIGIGNPPGTMDMKAFLLQKFSPTEREQIDASLEQGVEAMRTLILGGFTNKITRFNLGQKYK 240

[Close]

BLAST of CD860330 vs. TrEMBL
Match: D7TT16_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_6.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031009001 PE=4 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 1.681e-56
Identity = 102/137 (74.45%), Postives = 119/137 (86.86%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LLAKPQ YMNFSGESVGPLAA+Y+VPLRHIL+VYDE +LPNGVL+L P+ GHG+HNGLKNV+GHLDG  +FPRL IGIGNPPGTMD++A+LLQKFS  ERKQID + E G+ AVR L +NGFNH   RFNLGQKY+
Sbjct:  106 ILLAKPQAYMNFSGESVGPLAAYYQVPLRHILVVYDEMNLPNGVLRLAPRGGHGYHNGLKNVMGHLDGCREFPRLCIGIGNPPGTMDMKAYLLQKFSFEERKQIDAAQEQGIGAVRNLVLNGFNHSTTRFNLGQKYK 242

[Close]

BLAST of CD860330 vs. TrEMBL
Match: D7M817_ARALY (Peptidyl-tRNA hydrolase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_488464 PE=4 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 1.573e-54
Identity = 99/137 (72.26%), Postives = 120/137 (87.59%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LL KPQTYMNFSGESVG LA+HYRVPLRHIL++YDE +LPNGVL+LQPK G G+HNG+K+V+GHLDG  +FPRL+IGIG PPG MD++AFLLQKFS +ERKQIDE+LE G  AV+TL +NGFN  ++RFNL QKY+
Sbjct:   99 ILLVKPQTYMNFSGESVGSLASHYRVPLRHILMLYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLERKQIDEALEQGSEAVKTLVLNGFNQGISRFNLVQKYK 235

[Close]

BLAST of CD860330 vs. TrEMBL
Match: D7MK16_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_659872 PE=4 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 9.544e-52
Identity = 94/137 (68.61%), Postives = 113/137 (82.48%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LL KPQ YMNFSGESVGPLAA+Y++PLRHIL++YD+  LPNGVL+LQPK GH  HNGLKNV  HL+G   +PRL+IGIGNPPG MD++AFLLQKFS +ERKQID  LE GV  V+TL   GF+  ++RFNLGQKY+
Sbjct:  101 ILLVKPQAYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLPNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQIDAGLEQGVEGVKTLVEEGFSDSISRFNLGQKYK 237

[Close]

BLAST of CD860330 vs. TrEMBL
Match: B4FMU3_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 9.544e-52
Identity = 98/137 (71.53%), Postives = 116/137 (84.67%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            VL+ KPQ+YMN+SGE++GPLAA+Y+VPLRHILL+YD+TSLPNGVL+LQ K GHG HNGL+NVI HLDG  +FPRL+IGIG+PPG MD RAFLLQKFSS ER QID +LE GV AVRTL + GF+    RFNL QKY+
Sbjct:  116 VLVVKPQSYMNYSGEAIGPLAAYYQVPLRHILLIYDDTSLPNGVLRLQKKGGHGRHNGLQNVIEHLDGRREFPRLSIGIGSPPGKMDPRAFLLQKFSSEERVQIDTALEQGVDAVRTLVLKGFSGSTERFNLVQKYK 252

[Close]

BLAST of CD860330 vs. TrEMBL
Match: A8MQQ3_ARATH (Uncharacterized protein At5g38290.2 OS=Arabidopsis thaliana GN=At5g38290 PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 6.186e-51
Identity = 93/137 (67.88%), Postives = 113/137 (82.48%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LL KPQ YMNFSGESVGPLAA+Y++PLRHIL++YD+  L NGVL+LQPK GH  HNGLKNV  HL+G   +PRL+IGIGNPPG MD++AFLLQKFS +ERKQ+DE LE GV  V+TL   GF+  ++RFNLGQKY+
Sbjct:  110 ILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQKYK 246

[Close]

BLAST of CD860330 vs. TrEMBL
Match: C5XQU4_SORBI (Putative uncharacterized protein Sb03g007130 OS=Sorghum bicolor GN=Sb03g007130 PE=3 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 4.010e-50
Identity = 97/137 (70.80%), Postives = 114/137 (83.21%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            VL+ KPQ+YMN+SGE++GPLAA+Y+VPLRHILL+YD+ SLPNGVL+LQ K GHG HNGL+NVI +LDG  +FPRL+IGIG+PPG MD RAFLLQKFSS ER QID SLE GV AVRTL + GF     RFNL QKY+
Sbjct:  116 VLVVKPQSYMNYSGEAIGPLAAYYQVPLRHILLIYDDMSLPNGVLRLQKKGGHGRHNGLQNVIENLDGRREFPRLSIGIGSPPGKMDPRAFLLQKFSSEERVQIDASLEQGVDAVRTLVLKGFCGSTERFNLVQKYK 252

[Close]

BLAST of CD860330 vs. TrEMBL
Match: B7EEV8_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_00257 PE=2 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 9.876e-49
Identity = 94/137 (68.61%), Postives = 114/137 (83.21%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            VLL KPQ+Y+N+SGE++GPLAA+Y+VPLRHIL++YDE SLPNGVL+LQ K GHG HNGL+NV+  LD S + PRL+IGIG+PPG MD RAFLLQKFSS ER QID +LE GV AVRTL + GF+  + RFNL QKY+
Sbjct:  119 VLLVKPQSYINYSGEAIGPLAAYYQVPLRHILVMYDEMSLPNGVLRLQRKGGHGRHNGLQNVMECLDSSRELPRLSIGIGSPPGKMDTRAFLLQKFSSEERLQIDTALEQGVDAVRTLVLKGFSGSIERFNLVQKYK 255

[Close]

BLAST of CD860330 vs. SwissProt
Match: CRS2B_ARATH (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana GN=CRS2B PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 1.322e-55
Identity = 98/137 (71.53%), Postives = 121/137 (88.32%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LLAKPQTYMNFSGESVG LA+HYRVPLRHIL++YDE +LPNGVL+LQPK G G+HNG+K+V+GHLDG  +FPRL+IGIG PPG MD++AFLLQKFS +E+KQI+E+LE G  AV+TL +NGFN  ++RFNL QKY+
Sbjct:  100 ILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQGISRFNLVQKYK 236

[Close]

BLAST of CD860330 vs. SwissProt
Match: CRS2_MAIZE (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays GN=CRS2 PE=1 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 4.702e-53
Identity = 98/137 (71.53%), Postives = 116/137 (84.67%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            VL+ KPQ+YMN+SGE++GPLAA+Y+VPLRHILL+YD+TSLPNGVL+LQ K GHG HNGL+NVI HLDG  +FPRL+IGIG+PPG MD RAFLLQKFSS ER QID +LE GV AVRTL + GF+    RFNL QKY+
Sbjct:  116 VLVVKPQSYMNYSGEAIGPLAAYYQVPLRHILLIYDDTSLPNGVLRLQKKGGHGRHNGLQNVIEHLDGRREFPRLSIGIGSPPGKMDPRAFLLQKFSSEERVQIDTALEQGVDAVRTLVLKGFSGSTERFNLVQKYK 252

[Close]

BLAST of CD860330 vs. SwissProt
Match: CRS2A_ARATH (Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Arabidopsis thaliana GN=CRS2A PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 3.047e-52
Identity = 93/137 (67.88%), Postives = 113/137 (82.48%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LL KPQ YMNFSGESVGPLAA+Y++PLRHIL++YD+  L NGVL+LQPK GH  HNGLKNV  HL+G   +PRL+IGIGNPPG MD++AFLLQKFS +ERKQ+DE LE GV  V+TL   GF+  ++RFNLGQKY+
Sbjct:  106 ILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQKYK 242

[Close]

BLAST of CD860330 vs. SwissProt
Match: CRS2_ORYSJ (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza sativa subsp. japonica GN=Os01g0132800 PE=2 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 4.865e-50
Identity = 94/137 (68.61%), Postives = 114/137 (83.21%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            VLL KPQ+Y+N+SGE++GPLAA+Y+VPLRHIL++YDE SLPNGVL+LQ K GHG HNGL+NV+  LD S + PRL+IGIG+PPG MD RAFLLQKFSS ER QID +LE GV AVRTL + GF+  + RFNL QKY+
Sbjct:  119 VLLVKPQSYINYSGEAIGPLAAYYQVPLRHILVMYDEMSLPNGVLRLQRKGGHGRHNGLQNVMECLDSSRELPRLSIGIGSPPGKMDTRAFLLQKFSSEERLQIDTALEQGVDAVRTLVLKGFSGSIERFNLVQKYK 255

[Close]

BLAST of CD860330 vs. SwissProt
Match: PTHM_ARATH (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 8.608e-39
Identity = 76/137 (55.47%), Postives = 100/137 (72.99%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            V+LAKPQTYMN SGES GPLAA+Y++PL  +L+V+D+  LP GVL+LQ K GHG HNGLK+V+ H  G+ +F RL IGIG PPG MD +AFLLQKFS   R+++D++L  GV A++ +    F      FN+ QKY+
Sbjct:   73 VILAKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVMNHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDFGESWRLFNVEQKYK 209

[Close]

BLAST of CD860330 vs. SwissProt
Match: PTHC_ARATH (Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidopsis thaliana GN=At1g18440 PE=2 SV=2)

HSP 1 Score: 148.288 bits (373), Expect = 1.163e-35
Identity = 64/124 (51.61%), Postives = 93/124 (75.00%), Query Frame = -2

Query:   41 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFN 412
            ++LAKPQT+MN SGESVG + + Y++PL+ +L+VYD+  LP G L+L PK GHG HNG++++I  L GS DFPRL IGIG PPG MD   F+L++F+  E++++D + + G+ A+R L + GFN
Sbjct:  149 IMLAKPQTFMNLSGESVGQIVSFYKIPLKQVLVVYDDLDLPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQFNRQEQEELDHTFQTGLEAIRILLLEGFN 272

[Close]

BLAST of CD860330 vs. SwissProt
Match: PTHM_ORYSJ (Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sativa subsp. japonica GN=Os01g0693900 PE=2 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 8.338e-34
Identity = 64/134 (47.76%), Postives = 93/134 (69.40%), Query Frame = -2

Query:   11 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQ 412
            ++LAKPQT+MN SGESVG L +++++PL  +L++YD+  LP   L+L PK GHG HNG++++I HL  + DFPRL IGIG PPG MD   F+L+ F+  E++++D +   G+ AVR +A+ GFN      N  Q
Sbjct:  110 IMLAKPQTFMNASGESVGQLVSYFKIPLNQVLVMYDDLDLPFAKLRLLPKGGHGGHNGVRSIINHLKQNRDFPRLRIGIGRPPGKMDPANFVLRPFNRKEQEELDFAFHRGLEAVRIMALEGFNKSATYVNTAQ 243

[Close]

BLAST of CD860330 vs. SwissProt
Match: PTH_THEFY (Peptidyl-tRNA hydrolase OS=Thermobifida fusca (strain YX) GN=pth PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 5.989e-24
Identity = 55/122 (45.08%), Postives = 79/122 (64.75%), Query Frame = -2

Query:   47 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNG 412
            V+LAKP +YMN SG  V  L+A Y+VP+  I++V+DE  +P   LKL+   G   HNGL+++   L GS D+ R+ +GIG PPG MD  +F+LQ FS+ ERK++D  +     AV T+  +G
Sbjct:   89 VVLAKPASYMNLSGGPVAKLSAFYKVPVDRIIVVHDELDIPFARLKLKRGGGSAGHNGLRSITASL-GSPDYVRVRVGIGRPPGRMDAASFVLQDFSTAERKELDVHVARAADAVETVVTSG 209

[Close]

BLAST of CD860330 vs. SwissProt
Match: PTH_CLOPH (Peptidyl-tRNA hydrolase OS=Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) GN=pth PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 7.822e-24
Identity = 58/131 (44.27%), Postives = 80/131 (61.07%), Query Frame = -2

Query:   20 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFN 412
            V+LAKPQTYMN SGESV  L   Y+V    I+++YD+ SL  G L+++ K   G HNG+KN+I HL GS +F R+ IG+G+ P   DL  ++L +F   E   I E+LE    A  T+  +G    +N FN
Sbjct:   56 VILAKPQTYMNLSGESVRELIDFYKVTKEEIIVIYDDISLDVGQLRIRTKGSAGGHNGIKNIIAHL-GSDEFCRIKIGVGDKPKNWDLADYVLARFPKEEEPAIREALEKVSKACETILRDGAKVAMNLFN 185

[Close]

BLAST of CD860330 vs. SwissProt
Match: PTH_CHLAD (Peptidyl-tRNA hydrolase OS=Chloroflexus aggregans (strain MD-66 / DSM 9485) GN=pth PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.334e-23
Identity = 54/132 (40.91%), Postives = 80/132 (60.61%), Query Frame = -2

Query:   20 VLLAKPQTYMNFSGESVGPLAAHYRV-PLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFN 412
            V L KPQTYMN SG++V  L   Y++ P R +L++YD+  LP   L+L+ +   G HNG+++++G L G+ +FPRL IGIG PPG MD   ++L +F+  E   + E L     AV  +   G    +NR+N
Sbjct:   56 VALVKPQTYMNLSGQAVAALRQWYKIDPARELLVIYDDLDLPFAKLRLRERGSAGTHNGMRSIVGQL-GTTEFPRLRIGIGQPPGQMDAADYVLSRFTPEEEAVLPEVLARVADAVEVVVSEGLTAAMNRYN 186

[Close]

BLAST of CD860330 vs. TAIR peptide
Match: AT5G16140.2 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:5270308-5271517 REVERSE LENGTH=240)

HSP 1 Score: 214.542 bits (545), Expect = 1.539e-56
Identity = 98/137 (71.53%), Postives = 121/137 (88.32%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LLAKPQTYMNFSGESVG LA+HYRVPLRHIL++YDE +LPNGVL+LQPK G G+HNG+K+V+GHLDG  +FPRL+IGIG PPG MD++AFLLQKFS +E+KQI+E+LE G  AV+TL +NGFN  ++RFNL QKY+
Sbjct:  100 ILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQGISRFNLVQKYK 236

[Close]

BLAST of CD860330 vs. TAIR peptide
Match: AT5G16140.1 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:5270308-5271517 REVERSE LENGTH=240)

HSP 1 Score: 214.542 bits (545), Expect = 1.539e-56
Identity = 98/137 (71.53%), Postives = 121/137 (88.32%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LLAKPQTYMNFSGESVG LA+HYRVPLRHIL++YDE +LPNGVL+LQPK G G+HNG+K+V+GHLDG  +FPRL+IGIG PPG MD++AFLLQKFS +E+KQI+E+LE G  AV+TL +NGFN  ++RFNL QKY+
Sbjct:  100 ILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQGISRFNLVQKYK 236

[Close]

BLAST of CD860330 vs. TAIR peptide
Match: AT5G38290.1 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:15303603-15305381 FORWARD LENGTH=246)

HSP 1 Score: 203.371 bits (516), Expect = 3.548e-53
Identity = 93/137 (67.88%), Postives = 113/137 (82.48%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LL KPQ YMNFSGESVGPLAA+Y++PLRHIL++YD+  L NGVL+LQPK GH  HNGLKNV  HL+G   +PRL+IGIGNPPG MD++AFLLQKFS +ERKQ+DE LE GV  V+TL   GF+  ++RFNLGQKY+
Sbjct:  106 ILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQKYK 242

[Close]

BLAST of CD860330 vs. TAIR peptide
Match: AT5G38290.2 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:15303491-15305381 FORWARD LENGTH=250)

HSP 1 Score: 203.371 bits (516), Expect = 3.548e-53
Identity = 93/137 (67.88%), Postives = 113/137 (82.48%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            +LL KPQ YMNFSGESVGPLAA+Y++PLRHIL++YD+  L NGVL+LQPK GH  HNGLKNV  HL+G   +PRL+IGIGNPPG MD++AFLLQKFS +ERKQ+DE LE GV  V+TL   GF+  ++RFNLGQKY+
Sbjct:  110 ILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSDSISRFNLGQKYK 246

[Close]

BLAST of CD860330 vs. TAIR peptide
Match: AT5G19830.1 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr5:6703383-6705125 FORWARD LENGTH=219)

HSP 1 Score: 158.688 bits (400), Expect = 1.002e-39
Identity = 76/137 (55.47%), Postives = 100/137 (72.99%), Query Frame = -2

Query:    2 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFNHHVNRFNLGQKYQ 412
            V+LAKPQTYMN SGES GPLAA+Y++PL  +L+V+D+  LP GVL+LQ K GHG HNGLK+V+ H  G+ +F RL IGIG PPG MD +AFLLQKFS   R+++D++L  GV A++ +    F      FN+ QKY+
Sbjct:   73 VILAKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVMNHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDFGESWRLFNVEQKYK 209

[Close]

BLAST of CD860330 vs. TAIR peptide
Match: AT1G18440.1 (| Symbols: | Peptidyl-tRNA hydrolase family protein | chr1:6345994-6347686 FORWARD LENGTH=288)

HSP 1 Score: 148.288 bits (373), Expect = 1.355e-36
Identity = 64/124 (51.61%), Postives = 93/124 (75.00%), Query Frame = -2

Query:   41 VLLAKPQTYMNFSGESVGPLAAHYRVPLRHILLVYDETSLPNGVLKLQPKSGHGHHNGLKNVIGHLDGSHDFPRLAIGIGNPPGTMDLRAFLLQKFSSVERKQIDESLEPGVPAVRTLAMNGFN 412
            ++LAKPQT+MN SGESVG + + Y++PL+ +L+VYD+  LP G L+L PK GHG HNG++++I  L GS DFPRL IGIG PPG MD   F+L++F+  E++++D + + G+ A+R L + GFN
Sbjct:  149 IMLAKPQTFMNLSGESVGQIVSFYKIPLKQVLVVYDDLDLPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQFNRQEQEELDHTFQTGLEAIRILLLEGFN 272

The following BLAST results are available for this feature:

BLAST of CD860330 vs. TrEMBL
BLAST of CD860330 vs. SwissProt
BLAST of CD860330 vs. TAIR peptide

BLAST of CD860330 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TK07_SOYBN	1.329e-61	82.48	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9RSL9_RICCO	8.912e-58	75.18	Peptidyl-tRNA hydrolase, putative OS=Ricinus commu...	[more]
B9H311_POPTR	9.854e-57	72.99	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TT16_VITVI	1.681e-56	74.45	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7M817_ARALY	1.573e-54	72.26	Peptidyl-tRNA hydrolase family protein OS=Arabidop...	[more]
D7MK16_ARALY	9.544e-52	68.61	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
B4FMU3_MAIZE	9.544e-52	71.53	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
A8MQQ3_ARATH	6.186e-51	67.88	Uncharacterized protein At5g38290.2 OS=Arabidopsis...	[more]
C5XQU4_SORBI	4.010e-50	70.80	Putative uncharacterized protein Sb03g007130 OS=So...	[more]
B7EEV8_ORYSJ	9.876e-49	68.61	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of CD860330 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CRS2B_ARATH	1.322e-55	71.53	Chloroplastic group IIB intron splicing facilitato...	[more]
CRS2_MAIZE	4.702e-53	71.53	Chloroplastic group IIB intron splicing facilitato...	[more]
CRS2A_ARATH	3.047e-52	67.88	Chloroplastic group IIB intron splicing facilitato...	[more]
CRS2_ORYSJ	4.865e-50	68.61	Chloroplastic group IIB intron splicing facilitato...	[more]
PTHM_ARATH	8.608e-39	55.47	Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidop...	[more]
PTHC_ARATH	1.163e-35	51.61	Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidop...	[more]
PTHM_ORYSJ	8.338e-34	47.76	Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sa...	[more]
PTH_THEFY	5.989e-24	45.08	Peptidyl-tRNA hydrolase OS=Thermobifida fusca (str...	[more]
PTH_CLOPH	7.822e-24	44.27	Peptidyl-tRNA hydrolase OS=Clostridium phytofermen...	[more]
PTH_CHLAD	1.334e-23	40.91	Peptidyl-tRNA hydrolase OS=Chloroflexus aggregans ...	[more]

BLAST of CD860330 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT5G16140.2	1.539e-56	71.53	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT5G16140.1	1.539e-56	71.53	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT5G38290.1	3.548e-53	67.88	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT5G38290.2	3.548e-53	67.88	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT5G19830.1	1.002e-39	55.47	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]
AT1G18440.1	1.355e-36	51.61	\| Symbols: \| Peptidyl-tRNA hydrolase family prote...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001328	Peptidyl-tRNA hydrolase	GENE3D	3.40.50.1470	no description	coord: 1..132 score: 8
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 1..137 score: 8.4
IPR001328	Peptidyl-tRNA hydrolase	PFAM	PF01195	Pept_tRNA_hydro	coord: 1..131 score: 1.8
IPR001328	Peptidyl-tRNA hydrolase	SUPERFAMILY	53178	Peptidyl-tRNA hydrolase-like	coord: 1..134 score: 1.9
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01196	PEPT_TRNA_HYDROL_2	coord: 52..62 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD860330 ID=CD860330; Name=CD860330; organism=Pisum sativum; type=EST; length=413bp
ACTGGTATTTTTGCCCTAAGTTGAATCTATTCACATGATGGTTAAACCCA
TTCATCGCTAATGTCCTAACAGCTGGAACTCCTGGCTCCAATGACTCGTC
AATCTGCTTTCTTTCCACCGAACTGAACTTTTGTAGAAGAAACGCTCTTA
AGTCCATAGTTCCAGGAGGATTTCCAATTCCGATTGCAAGTCGAGGAAAA
TCATGGGAACCATCCAAATGGCCTATCACATTTTTCAATCCATTGTGATG
CCCATGTCCACTTTTAGGTTGAAGCTTCAAAACACCATTCGGAAGACTAG
TTTCATCATAAACTAGTAGAATATGACGCAAGGGCACTCGATAATGAGCA
GCAAGCGGCCCAACCGATTCGCCACTAAAATTCATGTAAGTTTGAGGCTT
TGCCAACAAAACC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004045	aminoacyl-tRNA hydrolase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR001328	Pept_tRNA_hydro
IPR018171	Pept_tRNA_hydro_CS

Search form