EX568960

EST Overview


Name	EX568960
Unique Name	EX568960
Type	EST
Organism	Pisum sativum (pea)
Sequence length	816

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of EX568960 vs. TrEMBL
Match: B9ZZP2_PEA (Ascorbate oxidase OS=Pisum sativum GN=PsAAO1 PE=2 SV=1)

HSP 1 Score: 451.44 bits (1160), Expect = 1.193e-134
Identity = 216/241 (89.63%), Postives = 216/241 (89.63%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            QALTFLNYKTISA            LWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFK             YVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK
Sbjct:  314 QALTFLNYKTISASVFPTSPPPVTPLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKNPVNPNTTTGNGVYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 554

HSP 2 Score: 54.6842 bits (130), Expect = 1.193e-134
Identity = 23/25 (92.00%), Postives = 25/25 (100.00%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNNGH 783
            +GK+GKVPPQALTCGATARFVNNGH
Sbjct:  549 VGKIGKVPPQALTCGATARFVNNGH 573

[Close]

BLAST of EX568960 vs. TrEMBL
Match: O24093_MEDTR (L-ascorbate oxidase OS=Medicago truncatula GN=MtN23 PE=2 SV=1)

HSP 1 Score: 392.119 bits (1006), Expect = 3.002e-107
Identity = 183/239 (76.57%), Postives = 196/239 (82.01%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKI 719
            QALT LNYK +SA            LWND + SKAFTKQIISKMG PQPPK SH+ I  LNTQN IG+FTKWAINNVSLTLP TPY+GS+KF LKNTFD+ PPP  FP+DYDIFK             Y FQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEG+FRPG+DE++FNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAE V K+
Sbjct:  314 QALTILNYKPLSASVFPTSPPPVTPLWNDTDHSKAFTKQIISKMGNPQPPKSSHRSIHFLNTQNKIGSFTKWAINNVSLTLPTTPYLGSIKFNLKNTFDKNPPPERFPMDYDIFKNPVNPNTTTGNGVYTFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGRFRPGVDERSFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEAVHKV 552

[Close]

BLAST of EX568960 vs. TrEMBL
Match: Q8LJU3_SOYBN (Ascorbate oxidase (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 368.622 bits (945), Expect = 3.236e-103
Identity = 174/241 (72.20%), Postives = 190/241 (78.84%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            Q LT LNYKTISA            LWNDF  SKAFTK+II+KMGTPQPPK   +++ LLNTQN +  FTKW+INNVSLTLP TPY+GS+KFK+ N FD+ PPP  FP DYDIF              Y+F LNEVVDVILQN+NQL+ NGSEIHPWHLHGHDFWVLGYGEGKF+ G DEK FNLT APLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGV K+GK
Sbjct:  169 QGLTILNYKTISASIFPTSSPPITPLWNDFEHSKAFTKKIIAKMGTPQPPKLYDRRVFLLNTQNRVDGFTKWSINNVSLTLPPTPYLGSIKFKINNAFDQTPPPMNFPQDYDIFNPPVNPNATIGNGVYMFNLNEVVDVILQNSNQLSVNGSEIHPWHLHGHDFWVLGYGEGKFKLG-DEKKFNLTHAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGK 408

HSP 2 Score: 32.7278 bits (73), Expect = 3.236e-103
Identity = 14/24 (58.33%), Postives = 18/24 (75.00%), Query Frame = 1

Query:  715 KLGKVPPQALTCGATA-RFVNNGH 783
            K+GK+P +ALTCG T  + V NGH
Sbjct:  405 KVGKIPREALTCGLTGKKLVENGH 428

[Close]

BLAST of EX568960 vs. TrEMBL
Match: D7M0Q2_ARALY (Ascorbate oxidase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_489021 PE=4 SV=1)

HSP 1 Score: 324.324 bits (830), Expect = 3.960e-89
Identity = 149/241 (61.83%), Postives = 175/241 (72.61%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            QALT LNY T  A             W+DF RSK F+K+I S MG+P PPK   ++++LLNTQNLI  +TKWAINNVSL  P TPY+GS+K+ LK  F+RK PPR++ +DYDI               YVF  N  VDVI+QNAN L G  SEIHPWHLHGHDFWVLGYG+GKF+PGIDEK +NL   PLRNTA+++PYGWTALRF  DNPGVW FHCHIEPHLHMGMGV+FAEG+ +IGK
Sbjct:  309 QALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGVVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 549

HSP 2 Score: 30.0314 bits (66), Expect = 3.960e-89
Identity = 11/23 (47.83%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNN 777
            L ++GK+P +AL CG T +F+ N
Sbjct:  544 LNRIGKIPDEALGCGLTKQFLMN 566

[Close]

BLAST of EX568960 vs. TrEMBL
Match: O04947_ARATH (Ascorbate oxidase (Fragment) OS=Arabidopsis thaliana GN=At5g21105 PE=2 SV=1)

HSP 1 Score: 323.553 bits (828), Expect = 5.165e-89
Identity = 148/241 (61.41%), Postives = 175/241 (72.61%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            QALT LNY T  A             W+DF RSK F+K+I S MG+P PPK   ++++LLNTQNLI  +TKWAINNVSL  P TPY+GS+K+ LK  F+RK PPR++ +DYDI               YVF  N  VDVI+QNAN L G  SEIHPWHLHGHDFWVLGYG+GKF+PGIDEK +NL   PLRNTA+++PYGWTA+RF  DNPGVW FHCHIEPHLHMGMGV+FAEG+ +IGK
Sbjct:  306 QALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 546

HSP 2 Score: 30.4166 bits (67), Expect = 5.165e-89
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNN 777
            L ++GKVP +AL CG T +F+ N
Sbjct:  541 LNRIGKVPDEALGCGLTKQFLMN 563

[Close]

BLAST of EX568960 vs. TrEMBL
Match: Q94A37_ARATH (AT5g21100/T10F18_130 OS=Arabidopsis thaliana GN=At5g21105 PE=2 SV=1)

HSP 1 Score: 323.553 bits (828), Expect = 5.167e-89
Identity = 148/241 (61.41%), Postives = 175/241 (72.61%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            QALT LNY T  A             W+DF RSK F+K+I S MG+P PPK   ++++LLNTQNLI  +TKWAINNVSL  P TPY+GS+K+ LK  F+RK PPR++ +DYDI               YVF  N  VDVI+QNAN L G  SEIHPWHLHGHDFWVLGYG+GKF+PGIDEK +NL   PLRNTA+++PYGWTA+RF  DNPGVW FHCHIEPHLHMGMGV+FAEG+ +IGK
Sbjct:  269 QALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 509

HSP 2 Score: 30.4166 bits (67), Expect = 5.167e-89
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNN 777
            L ++GKVP +AL CG T +F+ N
Sbjct:  504 LNRIGKVPDEALGCGLTKQFLMN 526

[Close]

BLAST of EX568960 vs. TrEMBL
Match: Q9SDV8_BRAJU (Ascorbate oxidase (Fragment) OS=Brassica juncea PE=2 SV=1)

HSP 1 Score: 323.168 bits (827), Expect = 8.789e-89
Identity = 145/240 (60.42%), Postives = 175/240 (72.92%), Query Frame = 3

Query:    6 ALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            ALT L+Y T  +             WNDF+RSK+F+++I++ MG+P PP+   ++++LLNTQN I   TKWA+NNVSL +P TPY+GS K++L+  FDRK PP TFP+DYDI               YVF  N  VDVILQNAN L+ N SEIHPWHLHGHDFWVLGYGEGKFRPG+DEK +NL   PL+NT  +FPYGWTALRF  DNPGVW FHCHIEPHLHMGMGV+FAEG+ +IGK
Sbjct:  314 ALTVLHYVTAPSSQPHSSPPPETPRWNDFDRSKSFSRKILAAMGSPPPPRKFKKRLILLNTQNTIDGATKWALNNVSLVVPATPYLGSGKYRLRRGFDRKSPPTTFPVDYDIMNPPRNRNTTKGNGIYVFPFNVTVDVILQNANGLDANASEIHPWHLHGHDFWVLGYGEGKFRPGVDEKTYNLKNPPLKNTVALFPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 553

HSP 2 Score: 30.0314 bits (66), Expect = 8.789e-89
Identity = 11/23 (47.83%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNN 777
            L ++GK+P +AL CG T +F+ N
Sbjct:  548 LNRIGKIPDEALGCGLTKQFLMN 570

[Close]

BLAST of EX568960 vs. TrEMBL
Match: Q0WN00_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 322.013 bits (824), Expect = 1.500e-88
Identity = 147/241 (61.00%), Postives = 175/241 (72.61%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            +ALT LNY T  A             W+DF RSK F+K+I S MG+P PPK   ++++LLNTQNLI  +TKWAINNVSL  P TPY+GS+K+ LK  F+RK PPR++ +DYDI               YVF  N  VDVI+QNAN L G  SEIHPWHLHGHDFWVLGYG+GKF+PGIDEK +NL   PLRNTA+++PYGWTA+RF  DNPGVW FHCHIEPHLHMGMGV+FAEG+ +IGK
Sbjct:  100 RALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 340

HSP 2 Score: 30.4166 bits (67), Expect = 1.500e-88
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNN 777
            L ++GKVP +AL CG T +F+ N
Sbjct:  335 LNRIGKVPDEALGCGLTKQFLMN 357

[Close]

BLAST of EX568960 vs. TrEMBL
Match: Q9SDV7_BRAJU (Ascorbate oxidase (Fragment) OS=Brassica juncea PE=2 SV=1)

HSP 1 Score: 321.627 bits (823), Expect = 2.545e-88
Identity = 144/240 (60.00%), Postives = 175/240 (72.92%), Query Frame = 3

Query:    6 ALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            ALT L+Y T  +             WNDF+RSK+F+++I++ MG+P PP+   ++++LLNTQN I   TKWA+N VSL +P TPY+GS+K++L+  FDRK PP TFP+DYDI               YVF  N  VDVILQNAN L+ N SEIHPWHLHGHDFWVLGYGEGKFRPG+DEK +NL   PL+NT  +FPYGWTALRF  DNPGVW FHCHIEPHLHMGMGV+FAEG+ +IGK
Sbjct:  313 ALTVLHYVTAPSSQPPSSPPPETPRWNDFDRSKSFSRKILAAMGSPPPPRKFKKRLILLNTQNTIDGATKWALNIVSLVVPATPYLGSVKYRLRRGFDRKSPPTTFPVDYDIMNPPRNRNTTKGNGIYVFPFNVTVDVILQNANGLDANASEIHPWHLHGHDFWVLGYGEGKFRPGVDEKTYNLKNPPLKNTVALFPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 552

HSP 2 Score: 30.0314 bits (66), Expect = 2.545e-88
Identity = 11/23 (47.83%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNN 777
            L ++GK+P +AL CG T +F+ N
Sbjct:  547 LNRIGKIPDEALGCGLTKQFLMN 569

[Close]

BLAST of EX568960 vs. TrEMBL
Match: B3IWI2_BRARC (Ascorbic acid oxidase OS=Brassica rapa subsp. chinensis GN=BcAAO PE=2 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 3.320e-88
Identity = 145/240 (60.42%), Postives = 174/240 (72.50%), Query Frame = 3

Query:    6 ALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            ALT L+Y T  +             WN+F+RS+ F+K+I S MG+P PP+   ++++LLNTQN+I   TKWA+NNVSL +P TPY+GS+K+KL+  FDRK PP TFP+DYD+               YVF  N  VDVILQNAN L+ N SEIHPWHLHGHDFWVLGYGEGKFRP IDEK +NL   PLRNT  ++PYGWTALRF  DNPGVW FHCHIEPHLHMGMGV+FAEG+ +IGK
Sbjct:  317 ALTVLHYVTAPSSQPPSSPPPETPRWNNFDRSRNFSKRIFSAMGSPPPPRKFKKRLILLNTQNMIDGATKWALNNVSLVVPATPYLGSVKYKLRRGFDRKSPPTTFPMDYDVMNPPRNRNTTKGNGIYVFPFNVTVDVILQNANGLDANASEIHPWHLHGHDFWVLGYGEGKFRPEIDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 556

HSP 2 Score: 30.4166 bits (67), Expect = 3.320e-88
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNN 777
            L ++GKVP +AL CG T +F+ N
Sbjct:  551 LNRIGKVPDEALGCGLTKQFLMN 573

[Close]

BLAST of EX568960 vs. SwissProt
Match: ASO_CUCSA (L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1)

HSP 1 Score: 303.523 bits (776), Expect = 3.692e-83
Identity = 143/238 (60.08%), Postives = 164/238 (68.91%), Query Frame = 3

Query:    9 LTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIG 722
            LT LNY   SA             W DF+RSK FT +I + MG+P+PP   ++++ LLNTQN I  F KWAINNVSL LP TPY+ ++K +L   F++ PPP TFP++YDI               Y F + E VDVILQNAN LN N SEIHPWHLHGHDFWVLGYGEGKF    DEK  NL   PLRNT VIFPYGWTA+RF ADNPGVWAFHCHIEPHLHMGMGV+FAEGV  +G
Sbjct:  328 LTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHMVG 565

HSP 2 Score: 26.9498 bits (58), Expect = 3.692e-83
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 1

Query:  718 LGKVPPQALTCGATARFVN 774
            +G +PP+AL CG+TA   N
Sbjct:  564 VGMIPPKALACGSTALVKN 582

[Close]

BLAST of EX568960 vs. SwissProt
Match: ASO_CUCMA (L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=1 SV=2)

HSP 1 Score: 301.597 bits (771), Expect = 6.282e-83
Identity = 141/239 (59.00%), Postives = 169/239 (70.71%), Query Frame = 3

Query:    9 LTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            LT LNY   S              W+DF+RSK FT +I + MG+P+PP  S+++I LLNTQN+I  + KWAIN+VSL LP TPY+G++KF L + FD+ PPP  FP DYDI               Y F++ E+VDVILQNAN +  N SEIHPWHLHGHDFWVLGYG+GKF    +E + NL   PLRNT VIFPYGWTA+RF ADNPGVWAFHCHIEPHLHMGMGV+FAEGV K+G+
Sbjct:  323 LTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGYGDGKF-TAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGR 560

HSP 2 Score: 28.1054 bits (61), Expect = 6.282e-83
Identity = 11/22 (50.00%), Postives = 17/22 (77.27%), Query Frame = 1

Query:  715 KLGKVPPQALTCGATAR-FVNN 777
            K+G++P +AL CG TA+  +NN
Sbjct:  557 KVGRIPTKALACGGTAKSLINN 578

[Close]

BLAST of EX568960 vs. SwissProt
Match: ASO_CUCPM (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo PE=1 SV=1)

HSP 1 Score: 296.59 bits (758), Expect = 1.988e-81
Identity = 139/239 (58.16%), Postives = 167/239 (69.87%), Query Frame = 3

Query:    9 LTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            LT LNY   S              W+DF+RSK FT +I + MG+P+PP   +++I LLNTQN+I  + KWAIN+VSL LP TPY+G++K+ L + FD+ PPP  FP DYDI               Y F++ EVVDVILQNAN +  N SE HPWHLHGHDFWVLGYG+GKF    +E + NL   PLRNT VIFPYGWTA+RF ADNPGVWAFHCHIEPHLHMGMGV+FAEGV K+G+
Sbjct:  293 LTLLNYLPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKF-SAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGR 530

HSP 2 Score: 28.1054 bits (61), Expect = 1.988e-81
Identity = 11/22 (50.00%), Postives = 17/22 (77.27%), Query Frame = 1

Query:  715 KLGKVPPQALTCGATAR-FVNN 777
            K+G++P +AL CG TA+  +NN
Sbjct:  527 KVGRIPTKALACGGTAKSLINN 548

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BLAST of EX568960 vs. SwissProt
Match: ASO_TOBAC (L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE=2 SV=1)

HSP 1 Score: 300.056 bits (767), Expect = 7.503e-81
Identity = 143/241 (59.34%), Postives = 168/241 (69.71%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            Q LT LNY   SA            LWND+N SK+F+ +I + MG+P+PP  +H++I+LLNTQN I  +TKWAINNVSL LP   Y+GS+++ + N FD KPPP  FP DYD+ K             Y+ + N  +D+ILQNAN L  + SEIHPWHLHGHDFWVLGYGEGKF    D K FNL   PLRNTAVIFP+GWTALRF  DNPGVWAFHCHIEPHLHMGMGVIFAEGV  + K
Sbjct:  319 QGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSPKPPPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGI-NAFDTKPPPDNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVLGYGEGKFSEK-DVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVHLVKK 557

HSP 2 Score: 22.7126 bits (47), Expect = 7.503e-81
Identity = 9/21 (42.86%), Postives = 14/21 (66.67%), Query Frame = 1

Query:  724 KVPPQALTCGATAR-FVNNGH 783
            K+P +AL CG T +  ++N H
Sbjct:  557 KIPKEALACGLTGKMLMSNKH 577

[Close]

BLAST of EX568960 vs. SwissProt
Match: LAC13_ARATH (Laccase-13 OS=Arabidopsis thaliana GN=LAC13 PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 4.317e-23
Identity = 74/224 (33.04%), Postives = 102/224 (45.54%), Query Frame = 3

Query:   81 WNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLL----------NTQNLIG-NFTKW--AINNVSLTLP-----VTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIF 698
            +ND   + AFT Q+ S      P +        +          NTQ   G N T++  +INNVS   P     +  Y       +  T     PP TF  DY                 Y  + N  V +ILQ+ + +    +E HP HLHG++F+V+G G G F P  D  +FNL   P RNT    P GW A+RF A+NPG W  HCHI+ H+  G+ ++F
Sbjct:  329 FNDTATAAAFTAQMKSPSKVKVPLEIDENLFFTVGLGLFNCPTPNTQRCQGPNGTRFTASINNVSFVFPKQNSIMQAYYQGTPTGVFTTDFPPTPPVTF--DYTGNVSRGLWQPTRGTKAYKLKFNSQVQIILQDTSIVT---TENHPMHLHGYEFYVVGTGVGNFNPNTDTSSFNLIDPPRRNTIGTPPGGWVAIRFVANNPGAWLMHCHIDSHIFWGLAMVF 547

[Close]

BLAST of EX568960 vs. SwissProt
Match: LAC12_ARATH (Laccase-12 OS=Arabidopsis thaliana GN=LAC12 PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 7.364e-23
Identity = 64/184 (34.78%), Postives = 89/184 (48.37%), Query Frame = 3

Query:  189 QNLIGNFTKWAINNVSLTLP-----VTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFA--EGVGKI 719
            Q L G     ++NNVS  LP     +  +   +       F  KPP +      +I +             Y  +    V V+LQ+ N +    SE HP HLHG+DF+++G G G F P  D   FNL   PLRNT  +   GW  +RF ADNPGVW  HCH++ H+  G+ + F    GVG++
Sbjct:  374 QGLNGTRFTASMNNVSFVLPSNFSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNISRALFQPVKGTKL--YKLKYGSRVQVVLQDTNIVT---SENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKWGLAMAFLVDNGVGEL 552

[Close]

BLAST of EX568960 vs. SwissProt
Match: LAC16_ARATH (Laccase-16 OS=Arabidopsis thaliana GN=LAC16 PE=2 SV=2)

HSP 1 Score: 106.686 bits (265), Expect = 1.641e-22
Identity = 62/161 (38.51%), Postives = 81/161 (50.31%), Query Frame = 3

Query:  222 INNVSLTLPVTPYIGSLKFKLKNTF-DRKPPPRTFPIDYDI-FKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIF 698
            INNV+ T+P T  + +  F +   F D  P   + P DY    K             Y    N  V ++LQN   +    S+ HP+HLHG +F+ +G G G F P  D K FNL     RNT  +   GWTA+RF ADNPGVW  HCH+E H   G+ + F
Sbjct:  387 INNVTFTMPKTALLQAHFFNISGVFTDDFPAKPSNPYDYTAPVKLGVNAATMKGTKLYRLPYNATVQIVLQNTAMIL---SDNHPFHLHGFNFFEVGRGLGNFNPEKDPKAFNLVDPVERNTVGVPAGGWTAIRFIADNPGVWFMHCHLELHTTWGLKMAF 544

[Close]

BLAST of EX568960 vs. SwissProt
Match: LAC2_ORYSJ (Laccase-2 OS=Oryza sativa subsp. japonica GN=LAC2 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 6.234e-22
Identity = 57/164 (34.76%), Postives = 84/164 (51.22%), Query Frame = 3

Query:  219 AINNVSLTLPVTPYIGSLKFKLKNTFDR----KPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIF 698
            ++NN++  +P T  + +  +  K  F      +PP R    +Y                      N  V+++LQ+ N L+    E HP+HLHG++F+V+G G G F P  D   +NL   P RNT  +   GWTA+RF+ADNPGVW  HCH+E H   G+ + F
Sbjct:  383 SLNNITFVMPRTALLQAHYYGQKGVFAADFPDRPPAR---FNYTGVPLTAGLGTSLGTRLSKIAYNATVELVLQDTNLLS---VESHPFHLHGYNFFVVGRGVGNFDPAKDPAKYNLVDPPERNTVGVPAGGWTAIRFRADNPGVWFLHCHLEVHTSWGLKMAF 540

[Close]

BLAST of EX568960 vs. SwissProt
Match: LAC11_ARATH (Laccase-11 OS=Arabidopsis thaliana GN=LAC11 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.369e-21
Identity = 58/165 (35.15%), Postives = 84/165 (50.91%), Query Frame = 3

Query:  219 AINNVSLTLPVTPYIGSLKFKLKNTF-----DRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIF 698
            +INN++  +P T  + +    +   F     DR  PP+ F  +Y                    + N  ++++LQ+ N L     E HP+HLHG++F+V+G G G F P  D   FNL   P RNT  +   GW A+RF+ADNPGVW  HCH+E H   G+ + F
Sbjct:  378 SINNITFIMPKTALLKAHYSNISGVFRTDFPDR--PPKAF--NYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQDTNLLT---VESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRFRADNPGVWFMHCHLEVHTMWGLKMAF 535

[Close]

BLAST of EX568960 vs. SwissProt
Match: LAC23_ORYSJ (Laccase-23 OS=Oryza sativa subsp. japonica GN=LAC23 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.041e-21
Identity = 48/113 (42.48%), Postives = 65/113 (57.52%), Query Frame = 3

Query:  387 YVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFA--EGVGKI 719
            Y  +    V V+LQ  N   G   E HP HLHG+DF++L  G G F  G D   FN+   P+RNT  +   GW  +RF ADNPGVW  HCH++ H+  G+ + F   +GVG++
Sbjct:  445 YKLKYGSAVQVVLQGTNIFAG---ENHPIHLHGYDFYILAEGLGNFDAGADTAKFNMEDPPMRNTVGVPVNGWAVIRFVADNPGVWLMHCHLDVHITWGLAMAFLVDDGVGEL 554

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT5G21105.1 (| Symbols: | Plant L-ascorbate oxidase | chr5:7172727-7177409 FORWARD LENGTH=588)

HSP 1 Score: 323.553 bits (828), Expect = 3.291e-91
Identity = 148/241 (61.41%), Postives = 175/241 (72.61%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            QALT LNY T  A             W+DF RSK F+K+I S MG+P PPK   ++++LLNTQNLI  +TKWAINNVSL  P TPY+GS+K+ LK  F+RK PPR++ +DYDI               YVF  N  VDVI+QNAN L G  SEIHPWHLHGHDFWVLGYG+GKF+PGIDEK +NL   PLRNTA+++PYGWTA+RF  DNPGVW FHCHIEPHLHMGMGV+FAEG+ +IGK
Sbjct:  327 QALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 567

HSP 2 Score: 30.4166 bits (67), Expect = 3.291e-91
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNN 777
            L ++GKVP +AL CG T +F+ N
Sbjct:  562 LNRIGKVPDEALGCGLTKQFLMN 584

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT5G21105.2 (| Symbols: | Plant L-ascorbate oxidase | chr5:7174321-7177409 FORWARD LENGTH=397)

HSP 1 Score: 323.553 bits (828), Expect = 3.298e-91
Identity = 148/241 (61.41%), Postives = 175/241 (72.61%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGK 725
            QALT LNY T  A             W+DF RSK F+K+I S MG+P PPK   ++++LLNTQNLI  +TKWAINNVSL  P TPY+GS+K+ LK  F+RK PPR++ +DYDI               YVF  N  VDVI+QNAN L G  SEIHPWHLHGHDFWVLGYG+GKF+PGIDEK +NL   PLRNTA+++PYGWTA+RF  DNPGVW FHCHIEPHLHMGMGV+FAEG+ +IGK
Sbjct:  136 QALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 376

HSP 2 Score: 30.4166 bits (67), Expect = 3.298e-91
Identity = 12/23 (52.17%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  709 LGKLGKVPPQALTCGATARFVNN 777
            L ++GKVP +AL CG T +F+ N
Sbjct:  371 LNRIGKVPDEALGCGLTKQFLMN 393

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT5G21100.1 (| Symbols: | Plant L-ascorbate oxidase | chr5:7168312-7170719 FORWARD LENGTH=573)

HSP 1 Score: 301.982 bits (772), Expect = 2.607e-82
Identity = 144/264 (54.55%), Postives = 182/264 (68.94%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPID-YDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGKSSSTSSYLWCYC*VCKQWTLVR 791
            QALT +NY   +             +WND +RSK+F+K+I +  G P+PP+ SH +++LLNTQNL  ++TKW+INNVSL++PVTPY+GS+++ LK+ +D K P +   +D YDI K             Y F    VVDVILQNAN L G  SEIHPWH+HGHDFWVLGYGEGKF+PGIDEK FNL   PLRNT V++P+GWTA+RF  DNPGVW FHCHIEPHLHMGMGV+F EGV +IGK       L C   + ++W + R
Sbjct:  310 QALTVINYVD-ATESRPSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQLILLNTQNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGKMEIPDEALGCG--LTRKWLMNR 570

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT5G21105.3 (| Symbols: | Plant L-ascorbate oxidase | chr5:7172727-7177657 FORWARD LENGTH=543)

HSP 1 Score: 266.544 bits (680), Expect = 1.214e-71
Identity = 124/212 (58.49%), Postives = 148/212 (69.81%), Query Frame = 3

Query:    3 QALTFLNYKTISAXXXXXXXXXXXXLWNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPG 638
            QALT LNY T  A             W+DF RSK F+K+I S MG+P PPK   ++++LLNTQNLI  +TKWAINNVSL  P TPY+GS+K+ LK  F+RK PPR++ +DYDI               YVF  N  VDVI+QNAN L G  SEIHPWHLHGHDFWVLGYG+GKF+PGIDEK +NL   PLRNTA+++PYGWTA+RF  DNPG
Sbjct:  327 QALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT4G39830.1 (| Symbols: | Cupredoxin superfamily protein | chr4:18479103-18481184 FORWARD LENGTH=582)

HSP 1 Score: 217.238 bits (552), Expect = 8.445e-57
Identity = 110/228 (48.25%), Postives = 138/228 (60.53%), Query Frame = 3

Query:   81 WNDFNRSKAFTKQIISKMGTPQP-PKYSHQKILLLNTQNLIGNFTKWAINNVSLTLPVTPYIGSLKFKLKNTFD-RKPPPRTFPI-DYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGKIGKSSSTSSYLWC 755
            WND     A +  I ++ G     P+ S + I+LLNTQN +  + +W++NNVS   P TPY+ +LK  L N FD R   P  +   +YDIF              Y  + N  VDVILQNAN +N N SE HPWHLHGHDFWVLGYGEGKF    D K +N      +NT  + P+GWTALRF+ADNPGVW+FHCHIE H  MGMG++F  G+ K+  SS  SS + C
Sbjct:  352 WNDTRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGIDKV--SSLPSSIMGC 577

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT5G07130.1 (| Symbols: LAC13 | laccase 13 | chr5:2210567-2212525 FORWARD LENGTH=569)

HSP 1 Score: 108.612 bits (270), Expect = 4.230e-24
Identity = 74/224 (33.04%), Postives = 102/224 (45.54%), Query Frame = 3

Query:   81 WNDFNRSKAFTKQIISKMGTPQPPKYSHQKILLL----------NTQNLIG-NFTKW--AINNVSLTLP-----VTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIF 698
            +ND   + AFT Q+ S      P +        +          NTQ   G N T++  +INNVS   P     +  Y       +  T     PP TF  DY                 Y  + N  V +ILQ+ + +    +E HP HLHG++F+V+G G G F P  D  +FNL   P RNT    P GW A+RF A+NPG W  HCHI+ H+  G+ ++F
Sbjct:  329 FNDTATAAAFTAQMKSPSKVKVPLEIDENLFFTVGLGLFNCPTPNTQRCQGPNGTRFTASINNVSFVFPKQNSIMQAYYQGTPTGVFTTDFPPTPPVTF--DYTGNVSRGLWQPTRGTKAYKLKFNSQVQIILQDTSIVT---TENHPMHLHGYEFYVVGTGVGNFNPNTDTSSFNLIDPPRRNTIGTPPGGWVAIRFVANNPGAWLMHCHIDSHIFWGLAMVF 547

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT5G05390.1 (| Symbols: LAC12 | laccase 12 | chr5:1594753-1597042 FORWARD LENGTH=565)

HSP 1 Score: 107.842 bits (268), Expect = 7.216e-24
Identity = 64/184 (34.78%), Postives = 89/184 (48.37%), Query Frame = 3

Query:  189 QNLIGNFTKWAINNVSLTLP-----VTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFA--EGVGKI 719
            Q L G     ++NNVS  LP     +  +   +       F  KPP +      +I +             Y  +    V V+LQ+ N +    SE HP HLHG+DF+++G G G F P  D   FNL   PLRNT  +   GW  +RF ADNPGVW  HCH++ H+  G+ + F    GVG++
Sbjct:  374 QGLNGTRFTASMNNVSFVLPSNFSLLQAHSNGIPGVFTTDFPSKPPVKFDYTGNNISRALFQPVKGTKL--YKLKYGSRVQVVLQDTNIVT---SENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKWGLAMAFLVDNGVGEL 552

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT5G58910.1 (| Symbols: LAC16 | laccase 16 | chr5:23789522-23791681 REVERSE LENGTH=523)

HSP 1 Score: 106.686 bits (265), Expect = 1.608e-23
Identity = 62/161 (38.51%), Postives = 81/161 (50.31%), Query Frame = 3

Query:  222 INNVSLTLPVTPYIGSLKFKLKNTF-DRKPPPRTFPIDYDI-FKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIF 698
            INNV+ T+P T  + +  F +   F D  P   + P DY    K             Y    N  V ++LQN   +    S+ HP+HLHG +F+ +G G G F P  D K FNL     RNT  +   GWTA+RF ADNPGVW  HCH+E H   G+ + F
Sbjct:  344 INNVTFTMPKTALLQAHFFNISGVFTDDFPAKPSNPYDYTAPVKLGVNAATMKGTKLYRLPYNATVQIVLQNTAMIL---SDNHPFHLHGFNFFEVGRGLGNFNPEKDPKAFNLVDPVERNTVGVPAGGWTAIRFIADNPGVWFMHCHLELHTTWGLKMAF 501

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT5G03260.1 (| Symbols: LAC11 | laccase 11 | chr5:777198-779338 REVERSE LENGTH=557)

HSP 1 Score: 102.834 bits (255), Expect = 2.321e-22
Identity = 58/165 (35.15%), Postives = 84/165 (50.91%), Query Frame = 3

Query:  219 AINNVSLTLPVTPYIGSLKFKLKNTF-----DRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIF 698
            +INN++  +P T  + +    +   F     DR  PP+ F  +Y                    + N  ++++LQ+ N L     E HP+HLHG++F+V+G G G F P  D   FNL   P RNT  +   GW A+RF+ADNPGVW  HCH+E H   G+ + F
Sbjct:  378 SINNITFIMPKTALLKAHYSNISGVFRTDFPDR--PPKAF--NYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQDTNLLT---VESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRFRADNPGVWFMHCHLEVHTMWGLKMAF 535

[Close]

BLAST of EX568960 vs. TAIR peptide
Match: AT5G01190.1 (| Symbols: LAC10 | laccase 10 | chr5:72392-74612 FORWARD LENGTH=558)

HSP 1 Score: 101.679 bits (252), Expect = 5.171e-22
Identity = 59/174 (33.91%), Postives = 82/174 (47.13%), Query Frame = 3

Query:  201 GNFTKW--AINNVSLTLPVTPYIGSLKFKLKNTFDRKPPPRTFPIDYDIFKXXXXXXXXXXXXXYVFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGEGKFRPGIDEKNFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHMGMGVIFAEGVGK 716
            GNF++   AINN++  +P T  + +  F L   +    P +   +     K             Y    N  V V+LQ+   +     E HP HLHG +F+V+G G G +    D   FNL     RNT  +   GW A+RF+ADNPGVW  HCH+E H   G+ + F    GK
Sbjct:  372 GNFSRVVAAINNITFKMPKTALLQAHYFNLTGIYTTDFPAKPRRVFDFTGKPPSNLATMKATKLYKLPYNSTVQVVLQDTGNV---APENHPIHLHGFNFFVVGLGTGNYNSKKDSNKFNLVDPVERNTVGVPSGGWAAIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVENGK 542

The following BLAST results are available for this feature:

BLAST of EX568960 vs. TrEMBL
BLAST of EX568960 vs. SwissProt
BLAST of EX568960 vs. TAIR peptide

BLAST of EX568960 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9ZZP2_PEA	1.193e-134	89.63	Ascorbate oxidase OS=Pisum sativum GN=PsAAO1 PE=2 ...	[more]
O24093_MEDTR	3.002e-107	76.57	L-ascorbate oxidase OS=Medicago truncatula GN=MtN2...	[more]
Q8LJU3_SOYBN	3.236e-103	72.20	Ascorbate oxidase (Fragment) OS=Glycine max PE=2 S...	[more]
D7M0Q2_ARALY	3.960e-89	61.83	Ascorbate oxidase OS=Arabidopsis lyrata subsp. lyr...	[more]
O04947_ARATH	5.165e-89	61.41	Ascorbate oxidase (Fragment) OS=Arabidopsis thalia...	[more]
Q94A37_ARATH	5.167e-89	61.41	AT5g21100/T10F18_130 OS=Arabidopsis thaliana GN=At...	[more]
Q9SDV8_BRAJU	8.789e-89	60.42	Ascorbate oxidase (Fragment) OS=Brassica juncea PE...	[more]
Q0WN00_ARATH	1.500e-88	61.00	Putative uncharacterized protein OS=Arabidopsis th...	[more]
Q9SDV7_BRAJU	2.545e-88	60.00	Ascorbate oxidase (Fragment) OS=Brassica juncea PE...	[more]
B3IWI2_BRARC	3.320e-88	60.42	Ascorbic acid oxidase OS=Brassica rapa subsp. chin...	[more]

BLAST of EX568960 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ASO_CUCSA	3.692e-83	60.08	L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1	[more]
ASO_CUCMA	6.282e-83	59.00	L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=...	[more]
ASO_CUCPM	1.988e-81	58.16	L-ascorbate oxidase OS=Cucurbita pepo var. melopep...	[more]
ASO_TOBAC	7.503e-81	59.34	L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE...	[more]
LAC13_ARATH	4.317e-23	33.04	Laccase-13 OS=Arabidopsis thaliana GN=LAC13 PE=2 S...	[more]
LAC12_ARATH	7.364e-23	34.78	Laccase-12 OS=Arabidopsis thaliana GN=LAC12 PE=2 S...	[more]
LAC16_ARATH	1.641e-22	38.51	Laccase-16 OS=Arabidopsis thaliana GN=LAC16 PE=2 S...	[more]
LAC2_ORYSJ	6.234e-22	34.76	Laccase-2 OS=Oryza sativa subsp. japonica GN=LAC2 ...	[more]
LAC11_ARATH	2.369e-21	35.15	Laccase-11 OS=Arabidopsis thaliana GN=LAC11 PE=2 S...	[more]
LAC23_ORYSJ	4.041e-21	42.48	Laccase-23 OS=Oryza sativa subsp. japonica GN=LAC2...	[more]

BLAST of EX568960 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G21105.1	3.291e-91	61.41	\| Symbols: \| Plant L-ascorbate oxidase \| chr5:717...	[more]
AT5G21105.2	3.298e-91	61.41	\| Symbols: \| Plant L-ascorbate oxidase \| chr5:717...	[more]
AT5G21100.1	2.607e-82	54.55	\| Symbols: \| Plant L-ascorbate oxidase \| chr5:716...	[more]
AT5G21105.3	1.214e-71	58.49	\| Symbols: \| Plant L-ascorbate oxidase \| chr5:717...	[more]
AT4G39830.1	8.445e-57	48.25	\| Symbols: \| Cupredoxin superfamily protein \| chr...	[more]
AT5G07130.1	4.230e-24	33.04	\| Symbols: LAC13 \| laccase 13 \| chr5:2210567-22125...	[more]
AT5G05390.1	7.216e-24	34.78	\| Symbols: LAC12 \| laccase 12 \| chr5:1594753-15970...	[more]
AT5G58910.1	1.608e-23	38.51	\| Symbols: LAC16 \| laccase 16 \| chr5:23789522-2379...	[more]
AT5G03260.1	2.321e-22	35.15	\| Symbols: LAC11 \| laccase 11 \| chr5:777198-779338...	[more]
AT5G01190.1	5.171e-22	33.91	\| Symbols: LAC10 \| laccase 10 \| chr5:72392-74612 F...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 212..232 score
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 217..228 score
IPR008972	Cupredoxin	GENE3D	2.60.40.420	no description	coord: 50..235 score: 7.4
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 49..234 score: 3
IPR011706	Multicopper oxidase, type 2	PFAM	PF07731	Cu-oxidase_2	coord: 112..235 score: 3.9
None	No IPR available	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 1..251 score: 3.6e
None	No IPR available	PANTHER	PTHR11709:SF10	ASCORBATE OXIDASE	coord: 1..251 score: 3.6e
None	No IPR available	SEG	seg	seg	coord: 14..25 score: NAcoord: 116..128 score
None	No IPR available	SEG	seg	seg	coord: 61..72 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX568960 ID=EX568960; Name=EX568960; organism=Pisum sativum; type=EST; length=816bp
CACAAGCCTTAACATTCCTCAACTACAAAACAATCTCCGCCTCCGTTTTC
CCAACTTCACCACCGCCAGTAACTCCATTATGGAACGATTTCAACCGTAG
CAAAGCCTTCACAAAACAAATCATTTCCAAAATGGGAACACCGCAGCCAC
CAAAATATTCACACCAGAAAATTCTCCTCCTCAACACACAAAACTTGATC
GGTAATTTCACCAAATGGGCTATCAACAATGTTTCATTGACATTACCAGT
TACACCTTACATAGGATCATTAAAGTTTAAACTGAAAAACACATTCGATA
GAAAACCGCCACCAAGAACATTTCCAATCGACTACGACATATTTAAGAAT
CCGGTTAATCCGAACACAACTACCGGTAACGGTGTTTATGTTTTTCAGTT
AAACGAGGTTGTTGATGTGATCTTACAGAATGCAAACCAGTTAAACGGGA
ATGGTAGTGAGATTCATCCTTGGCATTTGCATGGACATGATTTTTGGGTT
TTAGGATATGGAGAAGGAAAATTCAGACCAGGTATTGATGAGAAGAATTT
TAACTTGACACGTGCGCCTTTGAGGAACACAGCGGTTATATTTCCATATG
GTTGGACAGCTTTAAGGTTTAAGGCTGATAATCCAGGTGTTTGGGCGTTT
CATTGTCATATTGAACCTCATTTGCATATGGGAATGGGTGTTATTTTTGC
TGAGGGTGTTGGGAAAATTGGGAAAAGTTCCTCCACAAGCTCTTACTTGT
GGTGCTACTGCTAGGTTTGTAAACAATGGACACTAGTGAGAGTGTGAGGC
TAGTTATTCTTAATCA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005507	copper ion binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR002355	Cu_oxidase_Cu_BS
IPR008972	Cupredoxin
IPR011706	Cu-oxidase_C

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