EX569844

EST Overview


Name	EX569844
Unique Name	EX569844
Type	EST
Organism	Pisum sativum (pea)
Sequence length	548

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of EX569844 vs. TrEMBL
Match: B9HUM5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1092581 PE=4 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 7.419e-28
Identity = 65/98 (66.33%), Postives = 81/98 (82.65%), Query Frame = 3

Query:    3 VRAGNSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGG 296
            VRA NS+L  ++ EVN THAEL+KEL SVQGQ  AERSRCF LEA+I+ELQK+LES+QS+E++VQ LR++KSA +QE+E +A AQRQ SGGVWR   G
Sbjct:  416 VRAENSRLTEKVDEVNGTHAELSKELHSVQGQLAAERSRCFKLEAQIAELQKILESLQSIENEVQLLRRQKSALEQEIERSA-AQRQGSGGVWRWIAG 512

[Close]

BLAST of EX569844 vs. TrEMBL
Match: D7TM16_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_19.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016168001 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.003e-24
Identity = 60/99 (60.61%), Postives = 79/99 (79.80%), Query Frame = 3

Query:    3 VRAGNSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            V+A NS+L  +I ++N T+AEL+KEL SVQGQ V ERSRC  LEA+I+ELQK+LE++QS+ED+VQ LR++KS  ++ ME AA AQRQ SGGVWR   G+
Sbjct:  408 VKAENSRLKEKIDDINGTYAELSKELHSVQGQLVVERSRCSKLEAQIAELQKMLETLQSIEDEVQLLRRQKSMLERNMEVAA-AQRQGSGGVWRWIAGT 505

[Close]

BLAST of EX569844 vs. TrEMBL
Match: Q9LZ83_ARATH (AT5G04420 protein OS=Arabidopsis thaliana GN=T32M21_20 PE=1 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.601e-22
Identity = 56/93 (60.22%), Postives = 74/93 (79.57%), Query Frame = 3

Query:   15 NSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFG 293
            N+KL  +I EVNS+H EL++ELQSV+GQ ++ERSRCF LEA+I+ELQK LES QS+E +V+ LR+++SA D+E +   T QRQ S GVW LFG
Sbjct:  423 NAKLREKIDEVNSSHTELSQELQSVEGQLISERSRCFKLEAQIAELQKALESGQSIEAEVEMLRRQRSASDEEED--GTVQRQGSAGVWGLFG 513

[Close]

BLAST of EX569844 vs. TrEMBL
Match: Q945N7_ARATH (AT5g04420/T32M21_20 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.601e-22
Identity = 56/93 (60.22%), Postives = 74/93 (79.57%), Query Frame = 3

Query:   15 NSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFG 293
            N+KL  +I EVNS+H EL++ELQSV+GQ ++ERSRCF LEA+I+ELQK LES QS+E +V+ LR+++SA D+E +   T QRQ S GVW LFG
Sbjct:  423 NAKLREKIDEVNSSHTELSQELQSVEGQLISERSRCFKLEAQIAELQKALESGQSIEAEVEMLRRQRSASDEEED--GTVQRQGSAGVWGLFG 513

[Close]

BLAST of EX569844 vs. TrEMBL
Match: D7LXI5_ARALY (Kelch repeat-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_487223 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 6.083e-22
Identity = 55/93 (59.14%), Postives = 73/93 (78.49%), Query Frame = 3

Query:   15 NSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFG 293
            N+KL  +I EVNS+H EL++ELQSV+GQ ++ERSRCF LEA+I+ELQK LES QS+E +V+ LR+++S  D+  +   T QRQ SGGVW LFG
Sbjct:  423 NAKLREKIDEVNSSHTELSQELQSVEGQLISERSRCFKLEAQIAELQKALESGQSIEAEVEMLRRQRSVSDEGED--GTVQRQGSGGVWGLFG 513

[Close]

BLAST of EX569844 vs. TrEMBL
Match: B9HL60_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_765221 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 6.295e-19
Identity = 50/75 (66.67%), Postives = 62/75 (82.67%), Query Frame = 3

Query:    3 VRAGNSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFD 227
            VRA N +L  +I EVN THAEL+KEL SVQGQ VAERSRCF LEA+ +ELQ +LES+QS+E++VQ LR++KSA D
Sbjct:  101 VRADNFRLTEKIDEVNGTHAELSKELHSVQGQLVAERSRCFKLEAQTAELQMMLESLQSIENEVQLLRRQKSASD 175

[Close]

BLAST of EX569844 vs. TrEMBL
Match: Q6K9X9_ORYSJ (Acyl-CoA binding protein-like OS=Oryza sativa subsp. japonica GN=OJ1136_C04.2 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 6.514e-16
Identity = 50/99 (50.51%), Postives = 64/99 (64.65%), Query Frame = 3

Query:    3 VRAGNSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            VR  NSKL   +  V  +H ELTKEL+SVQ Q  AE SRC  LE++I+   K LES  S+E++++ LRQ+ S  +Q M    TAQRQ SGGVW+   GS
Sbjct:  417 VRDENSKLKDRLDMVKLSHGELTKELKSVQHQLAAEGSRCQKLESQIAAAHKRLESTDSLENELEVLRQQISQVEQTM---TTAQRQKSGGVWKWVAGS 512

[Close]

BLAST of EX569844 vs. TrEMBL
Match: A2XB60_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_09500 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 6.514e-16
Identity = 50/99 (50.51%), Postives = 64/99 (64.65%), Query Frame = 3

Query:    3 VRAGNSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            VR  NSKL   +  V  +H ELTKEL+SVQ Q  AE SRC  LE++I+   K LES  S+E++++ LRQ+ S  +Q M    TAQRQ SGGVW+   GS
Sbjct:  377 VRDENSKLKDRLDMVKLSHGELTKELKSVQHQLAAEGSRCQKLESQIAAAHKRLESTDSLENELEVLRQQISLVEQTM---TTAQRQKSGGVWKWVAGS 472

[Close]

BLAST of EX569844 vs. TrEMBL
Match: C0PPX6_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 7.202e-15
Identity = 41/94 (43.62%), Postives = 69/94 (73.40%), Query Frame = 3

Query:   18 SKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            S+L  E+ E  + +A+LT+EL+SV+GQ  +E+SRCF LE  ++EL++ ++ M++++ +V+ L+++K+A DQ   +A  A+RQSSGGVW    GS
Sbjct:  565 SRLKQELAEAQTANADLTEELRSVRGQLASEQSRCFRLEVDVAELRQKMQQMEALQKEVELLQRQKAASDQAAANA--ARRQSSGGVWSWIAGS 656

[Close]

BLAST of EX569844 vs. TrEMBL
Match: C5WTY5_SORBI (Putative uncharacterized protein Sb01g002200 OS=Sorghum bicolor GN=Sb01g002200 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.096e-14
Identity = 43/93 (46.24%), Postives = 66/93 (70.97%), Query Frame = 3

Query:   21 KLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            +L  +I +  + +AELTKELQ+V+GQ  +E+SRCF LE  ++EL++ L+SM ++E +V+ LR++K+A +Q    A   QRQSSGG+W    GS
Sbjct:  564 QLKEDIAQAETRNAELTKELQAVRGQLASEQSRCFKLEVDVAELRQKLQSMDALEKEVELLRRQKAASEQAALDA--KQRQSSGGMWGWLAGS 654

[Close]

BLAST of EX569844 vs. SwissProt
Match: ACBP5_ARATH (Acyl-CoA-binding domain-containing protein 5 OS=Arabidopsis thaliana GN=ACBP5 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.461e-13
Identity = 38/93 (40.86%), Postives = 64/93 (68.82%), Query Frame = 3

Query:   21 KLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            +L  E+ E+++ + EL KELQSV+ Q  AE+SRCF LE +++EL++ L++M++++ +++ L Q++ A   E      A+RQSSGGVW    G+
Sbjct:  552 QLKEELAEIDTRNTELYKELQSVRNQLAAEQSRCFKLEVEVAELRQKLQTMETLQKELELL-QRQRAVASEQAATMNAKRQSSGGVWGWLAGT 643

[Close]

BLAST of EX569844 vs. SwissProt
Match: ACBP4_ARATH (Acyl-CoA-binding domain-containing protein 4 OS=Arabidopsis thaliana GN=ACBP4 PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.909e-13
Identity = 38/93 (40.86%), Postives = 64/93 (68.82%), Query Frame = 3

Query:   21 KLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            +L  E+ E    + +L KELQSV+GQ  AE+SRCF LE  ++EL++ L+++++++ +++ L+++K+A +Q    A  A+RQ SGGVW    GS
Sbjct:  570 QLRQELGEAELRNTDLYKELQSVRGQLAAEQSRCFKLEVDVAELRQKLQTLETLQKELELLQRQKAASEQA---AMNAKRQGSGGVWGWLAGS 659

[Close]

BLAST of EX569844 vs. TAIR peptide
Match: AT5G04420.3 (| Symbols: | Galactose oxidase/kelch repeat superfamily protein | chr5:1246867-1249455 REVERSE LENGTH=514)

HSP 1 Score: 109.383 bits (272), Expect = 1.289e-24
Identity = 56/93 (60.22%), Postives = 74/93 (79.57%), Query Frame = 3

Query:   15 NSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFG 293
            N+KL  +I EVNS+H EL++ELQSV+GQ ++ERSRCF LEA+I+ELQK LES QS+E +V+ LR+++SA D+E +   T QRQ S GVW LFG
Sbjct:  423 NAKLREKIDEVNSSHTELSQELQSVEGQLISERSRCFKLEAQIAELQKALESGQSIEAEVEMLRRQRSASDEEED--GTVQRQGSAGVWGLFG 513

[Close]

BLAST of EX569844 vs. TAIR peptide
Match: AT5G04420.2 (| Symbols: | Galactose oxidase/kelch repeat superfamily protein | chr5:1246867-1249455 REVERSE LENGTH=514)

HSP 1 Score: 109.383 bits (272), Expect = 1.289e-24
Identity = 56/93 (60.22%), Postives = 74/93 (79.57%), Query Frame = 3

Query:   15 NSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFG 293
            N+KL  +I EVNS+H EL++ELQSV+GQ ++ERSRCF LEA+I+ELQK LES QS+E +V+ LR+++SA D+E +   T QRQ S GVW LFG
Sbjct:  423 NAKLREKIDEVNSSHTELSQELQSVEGQLISERSRCFKLEAQIAELQKALESGQSIEAEVEMLRRQRSASDEEED--GTVQRQGSAGVWGLFG 513

[Close]

BLAST of EX569844 vs. TAIR peptide
Match: AT5G04420.1 (| Symbols: | Galactose oxidase/kelch repeat superfamily protein | chr5:1246867-1249455 REVERSE LENGTH=514)

HSP 1 Score: 109.383 bits (272), Expect = 1.289e-24
Identity = 56/93 (60.22%), Postives = 74/93 (79.57%), Query Frame = 3

Query:   15 NSKLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFG 293
            N+KL  +I EVNS+H EL++ELQSV+GQ ++ERSRCF LEA+I+ELQK LES QS+E +V+ LR+++SA D+E +   T QRQ S GVW LFG
Sbjct:  423 NAKLREKIDEVNSSHTELSQELQSVEGQLISERSRCFKLEAQIAELQKALESGQSIEAEVEMLRRQRSASDEEED--GTVQRQGSAGVWGLFG 513

[Close]

BLAST of EX569844 vs. TAIR peptide
Match: AT5G27630.1 (| Symbols: ACBP5 | acyl-CoA binding protein 5 | chr5:9776101-9780780 FORWARD LENGTH=648)

HSP 1 Score: 75.8702 bits (185), Expect = 1.580e-14
Identity = 38/93 (40.86%), Postives = 64/93 (68.82%), Query Frame = 3

Query:   21 KLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            +L  E+ E+++ + EL KELQSV+ Q  AE+SRCF LE +++EL++ L++M++++ +++ L Q++ A   E      A+RQSSGGVW    G+
Sbjct:  552 QLKEELAEIDTRNTELYKELQSVRNQLAAEQSRCFKLEVEVAELRQKLQTMETLQKELELL-QRQRAVASEQAATMNAKRQSSGGVWGWLAGT 643

[Close]

BLAST of EX569844 vs. TAIR peptide
Match: AT3G05420.2 (| Symbols: ACBP4 | acyl-CoA binding protein 4 | chr3:1561880-1567047 FORWARD LENGTH=669)

HSP 1 Score: 75.485 bits (184), Expect = 2.063e-14
Identity = 38/93 (40.86%), Postives = 64/93 (68.82%), Query Frame = 3

Query:   21 KLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            +L  E+ E    + +L KELQSV+GQ  AE+SRCF LE  ++EL++ L+++++++ +++ L+++K+A +Q    A  A+RQ SGGVW    GS
Sbjct:  571 QLRQELGEAELRNTDLYKELQSVRGQLAAEQSRCFKLEVDVAELRQKLQTLETLQKELELLQRQKAASEQA---AMNAKRQGSGGVWGWLAGS 660

[Close]

BLAST of EX569844 vs. TAIR peptide
Match: AT3G05420.1 (| Symbols: ACBP4 | acyl-CoA binding protein 4 | chr3:1561880-1567047 FORWARD LENGTH=668)

HSP 1 Score: 75.485 bits (184), Expect = 2.063e-14
Identity = 38/93 (40.86%), Postives = 64/93 (68.82%), Query Frame = 3

Query:   21 KLGGEIHEVNSTHAELTKELQSVQGQPVAERSRCFNLEAKISELQKLLESMQSVEDQVQALRQKKSAFDQEMEHAATAQRQSSGGVWRLFGGS 299
            +L  E+ E    + +L KELQSV+GQ  AE+SRCF LE  ++EL++ L+++++++ +++ L+++K+A +Q    A  A+RQ SGGVW    GS
Sbjct:  570 QLRQELGEAELRNTDLYKELQSVRGQLAAEQSRCFKLEVDVAELRQKLQTLETLQKELELLQRQKAASEQA---AMNAKRQGSGGVWGWLAGS 659

The following BLAST results are available for this feature:

BLAST of EX569844 vs. TrEMBL
BLAST of EX569844 vs. SwissProt
BLAST of EX569844 vs. TAIR peptide

BLAST of EX569844 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HUM5_POPTR	7.419e-28	66.33	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TM16_VITVI	1.003e-24	60.61	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9LZ83_ARATH	1.601e-22	60.22	AT5G04420 protein OS=Arabidopsis thaliana GN=T32M2...	[more]
Q945N7_ARATH	1.601e-22	60.22	AT5g04420/T32M21_20 OS=Arabidopsis thaliana PE=2 S...	[more]
D7LXI5_ARALY	6.083e-22	59.14	Kelch repeat-containing protein OS=Arabidopsis lyr...	[more]
B9HL60_POPTR	6.295e-19	66.67	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q6K9X9_ORYSJ	6.514e-16	50.51	Acyl-CoA binding protein-like OS=Oryza sativa subs...	[more]
A2XB60_ORYSI	6.514e-16	50.51	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C0PPX6_PICSI	7.202e-15	43.62	Putative uncharacterized protein OS=Picea sitchens...	[more]
C5WTY5_SORBI	2.096e-14	46.24	Putative uncharacterized protein Sb01g002200 OS=So...	[more]

BLAST of EX569844 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
ACBP5_ARATH	1.461e-13	40.86	Acyl-CoA-binding domain-containing protein 5 OS=Ar...	[more]
ACBP4_ARATH	1.909e-13	40.86	Acyl-CoA-binding domain-containing protein 4 OS=Ar...	[more]

BLAST of EX569844 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT5G04420.3	1.289e-24	60.22	\| Symbols: \| Galactose oxidase/kelch repeat super...	[more]
AT5G04420.2	1.289e-24	60.22	\| Symbols: \| Galactose oxidase/kelch repeat super...	[more]
AT5G04420.1	1.289e-24	60.22	\| Symbols: \| Galactose oxidase/kelch repeat super...	[more]
AT5G27630.1	1.580e-14	40.86	\| Symbols: ACBP5 \| acyl-CoA binding protein 5 \| ch...	[more]
AT3G05420.2	2.063e-14	40.86	\| Symbols: ACBP4 \| acyl-CoA binding protein 4 \| ch...	[more]
AT3G05420.1	2.063e-14	40.86	\| Symbols: ACBP4 \| acyl-CoA binding protein 4 \| ch...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR23244	KELCH REPEAT DOMAIN	coord: 2..71 score: 6.8
None	No IPR available	PANTHER	PTHR23244:SF29	RANGAP1-INTERACTING PROTEIN-RELATED	coord: 2..71 score: 6.8

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX569844 ID=EX569844; Name=EX569844; organism=Pisum sativum; type=EST; length=548bp
AAGTCAGGGCCGGAAATTCTAAGCTTGGAGGTGAGATACATGAAGTAAAT
AGTACTCATGCTGAATTGACCAAGGAACTCCAATCAGTTCAGGGACAACC
TGTGGCTGAGAGATCAAGATGCTTTAACCTGGAGGCCAAAATTTCAGAAC
TCCAAAAGTTGCTTGAATCAATGCAGTCTGTGGAAGATCAGGTACAGGCT
CTTCGGCAAAAGAAGTCTGCTTTTGACCAGGAGATGGAGCATGCTGCAAC
TGCTCAGAGGCAGAGTTCTGGTGGAGTTTGGCGATTGTTTGGTGGGAGTG
AAAAACGATGACTAAGGGCAATGCATCTTAAAGTGATTTGGGAGTTTATT
CTGAGAATTTTGCACTTATTTTTTTCAAGTAGCTGATTATTTTATGTTAT
TTAAGTTCATGCAGCAAATGACATTTCCTTGCTACAGAGGTTTTGGGCCC
TCATCTTGTGTTGCAATGCATGATGGATATTGAGCTCCAATTTTGATGGA
CTGCAACTTTCAAAAGACGGGTTCACTTTGTCTGGCTCATTTCATACT

Search form