EX570107

EST Overview


Name	EX570107
Unique Name	EX570107
Type	EST
Organism	Pisum sativum (pea)
Sequence length	351

Libraries


Library Name	Type
LIBEST_021973 Pisum sativum shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of EX570107 vs. TrEMBL
Match: B9SNT9_RICCO (Trehalose-6-phosphate synthase, putative OS=Ricinus communis GN=RCOM_1278420 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 7.830e-14
Identity = 42/49 (85.71%), Postives = 44/49 (89.80%), Query Frame = 3

Query:    3 MFGVITSARA--SLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI SARA  SLSP+A+VF CTVGQKPSKAKYYLEDTSEILRMLQGL
Sbjct:  737 MFEVIMSARAGPSLSPVAEVFACTVGQKPSKAKYYLEDTSEILRMLQGL 785

[Close]

BLAST of EX570107 vs. TrEMBL
Match: B9PB85_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_794676 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.886e-13
Identity = 41/49 (83.67%), Postives = 44/49 (89.80%), Query Frame = 3

Query:    3 MFGVITSARA--SLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI SAR+  SLSPIA+VF CTVG+KPSKAKYYLEDTSEILRMLQGL
Sbjct:  151 MFEVILSARSGPSLSPIAEVFACTVGRKPSKAKYYLEDTSEILRMLQGL 199

[Close]

BLAST of EX570107 vs. TrEMBL
Match: B9GZA1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_757196 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.075e-13
Identity = 40/49 (81.63%), Postives = 44/49 (89.80%), Query Frame = 3

Query:    3 MFGVITSARA--SLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI SAR+  SLSP+A+VF CTVG+KPSKAKYYLEDTSEILRMLQGL
Sbjct:  790 MFEVIMSARSGPSLSPVAEVFACTVGRKPSKAKYYLEDTSEILRMLQGL 838

[Close]

BLAST of EX570107 vs. TrEMBL
Match: Q5D6D9_GINBI (Trehalose-6-phosphate synthase OS=Ginkgo biloba PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.611e-12
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 3

Query:    3 MFGVITSARA--SLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF  ITSA A  SLSPIA+VF CTVGQKPSKAKYYL+DT E+LRMLQGL
Sbjct:  791 MFEGITSAMAGPSLSPIAEVFACTVGQKPSKAKYYLDDTVEVLRMLQGL 839

[Close]

BLAST of EX570107 vs. TrEMBL
Match: D7TTU3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_12.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00013122001 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.250e-11
Identity = 37/49 (75.51%), Postives = 42/49 (85.71%), Query Frame = 3

Query:    3 MFGVITSARA--SLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI  A+   SLSP+A+VF CTVG+KPSKAKYYLEDT+EILRMLQGL
Sbjct:  189 MFEVIIRAKGLPSLSPVAEVFACTVGRKPSKAKYYLEDTTEILRMLQGL 237

[Close]

BLAST of EX570107 vs. TrEMBL
Match: A5C0J5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_016593 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.250e-11
Identity = 37/49 (75.51%), Postives = 42/49 (85.71%), Query Frame = 3

Query:    3 MFGVITSARA--SLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI  A+   SLSP+A+VF CTVG+KPSKAKYYLEDT+EILRMLQGL
Sbjct:  792 MFEVIIRAKGLPSLSPVAEVFACTVGRKPSKAKYYLEDTTEILRMLQGL 840

[Close]

BLAST of EX570107 vs. TrEMBL
Match: Q56YF4_ARATH (Trehalose-6-phosphate synthase like protein OS=Arabidopsis thaliana GN=At4g17770 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.204e-11
Identity = 34/49 (69.39%), Postives = 42/49 (85.71%), Query Frame = 3

Query:    3 MFGVITSAR--ASLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI SA+   +LSP+A++F CTVGQKPSKAKYYL+DT+EI+RML GL
Sbjct:  103 MFEVIMSAKDGPALSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLDGL 151

[Close]

BLAST of EX570107 vs. TrEMBL
Match: D7MDE9_ARALY (ATTPS5 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_329750 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.103e-11
Identity = 33/49 (67.35%), Postives = 43/49 (87.76%), Query Frame = 3

Query:    3 MFGVITSAR--ASLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI +A+   +LSP+A++F CTVGQKPSKAKYYL+DT+EI+RML+GL
Sbjct:  791 MFEVIMTAKDGPALSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLEGL 839

[Close]

BLAST of EX570107 vs. TrEMBL
Match: Q2A970_BRAOL (Trehalose-6-phosphate synthase, putative OS=Brassica oleracea GN=40.t00052 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.058e-10
Identity = 33/49 (67.35%), Postives = 42/49 (85.71%), Query Frame = 3

Query:    3 MFGVITSAR--ASLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI  A+   +LSP+A++F CTVGQKPSKAKYYL+DT+EI+RML+GL
Sbjct:  791 MFEVIMGAKDGPALSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLEGL 839

[Close]

BLAST of EX570107 vs. TrEMBL
Match: B9SNF1_RICCO (Trehalose-6-phosphate synthase, putative OS=Ricinus communis GN=RCOM_1148340 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 6.859e-10
Identity = 33/49 (67.35%), Postives = 43/49 (87.76%), Query Frame = 3

Query:    3 MFGVITSARA--SLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VITS+ A  S++P A+VF CTVG+KPSKAKYYL+DT+EI+R++QGL
Sbjct:  695 MFEVITSSVAGPSIAPRAEVFACTVGKKPSKAKYYLDDTAEIVRLMQGL 743

[Close]

BLAST of EX570107 vs. SwissProt
Match: TPS5_ARATH (Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 5 OS=Arabidopsis thaliana GN=TPS5 PE=1 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 3.127e-12
Identity = 34/49 (69.39%), Postives = 42/49 (85.71%), Query Frame = 3

Query:    3 MFGVITSAR--ASLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI SA+   +LSP+A++F CTVGQKPSKAKYYL+DT+EI+RML GL
Sbjct:  791 MFEVIMSAKDGPALSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLDGL 839

[Close]

BLAST of EX570107 vs. SwissProt
Match: TPS6_ARATH (Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6 OS=Arabidopsis thaliana GN=TPS6 PE=1 SV=2)

HSP 1 Score: 62.7734 bits (151), Expect = 6.520e-10
Identity = 30/49 (61.22%), Postives = 40/49 (81.63%), Query Frame = 3

Query:    3 MFGVITSARA--SLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI S+    S++P A++F CTVGQKPSKAKYYL+DT+EI+R++ GL
Sbjct:  802 MFEVICSSTEGPSIAPRAEIFACTVGQKPSKAKYYLDDTTEIVRLMHGL 850

[Close]

BLAST of EX570107 vs. TAIR peptide
Match: AT4G17770.1 (| Symbols: ATTPS5, TPS5 | trehalose phosphatase/synthase 5 | chr4:9877055-9880084 FORWARD LENGTH=862)

HSP 1 Score: 70.4774 bits (171), Expect = 2.405e-13
Identity = 34/49 (69.39%), Postives = 42/49 (85.71%), Query Frame = 3

Query:    3 MFGVITSAR--ASLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI SA+   +LSP+A++F CTVGQKPSKAKYYL+DT+EI+RML GL
Sbjct:  791 MFEVIMSAKDGPALSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLDGL 839

[Close]

BLAST of EX570107 vs. TAIR peptide
Match: AT1G68020.2 (| Symbols: ATTPS6, TPS6 | UDP-Glycosyltransferase / trehalose-phosphatase family protein | chr1:25497493-25500241 FORWARD LENGTH=860)

HSP 1 Score: 62.7734 bits (151), Expect = 5.014e-11
Identity = 30/49 (61.22%), Postives = 40/49 (81.63%), Query Frame = 3

Query:    3 MFGVITSARA--SLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF VI S+    S++P A++F CTVGQKPSKAKYYL+DT+EI+R++ GL
Sbjct:  802 MFEVICSSTEGPSIAPRAEIFACTVGQKPSKAKYYLDDTTEIVRLMHGL 850

[Close]

BLAST of EX570107 vs. TAIR peptide
Match: AT2G18700.1 (| Symbols: ATTPS11, TPS11, ATTPSB | trehalose phosphatase/synthase 11 | chr2:8109043-8111799 FORWARD LENGTH=862)

HSP 1 Score: 51.2174 bits (121), Expect = 1.510e-7
Identity = 21/33 (63.64%), Postives = 28/33 (84.85%), Query Frame = 3

Query:   45 IADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            + +VF CTVGQKPSKAKYYL+DT  +++ML+ L
Sbjct:  799 LEEVFACTVGQKPSKAKYYLDDTPSVIKMLEWL 831

[Close]

BLAST of EX570107 vs. TAIR peptide
Match: AT1G06410.1 (| Symbols: ATTPS7, TPS7, ATTPSA | trehalose-phosphatase/synthase 7 | chr1:1955413-1958153 FORWARD LENGTH=851)

HSP 1 Score: 51.2174 bits (121), Expect = 1.510e-7
Identity = 28/49 (57.14%), Postives = 34/49 (69.39%), Query Frame = 3

Query:    3 MFGVITSARAS--LSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            MF  I +A +   L   A VF CTVGQKPSKAKYYL+DT+E+  ML+ L
Sbjct:  785 MFEAIGNAMSKRLLCDNALVFACTVGQKPSKAKYYLDDTTEVTCMLESL 833

[Close]

BLAST of EX570107 vs. TAIR peptide
Match: AT1G60140.1 (| Symbols: ATTPS10, TPS10 | trehalose phosphate synthase | chr1:22177246-22180073 REVERSE LENGTH=861)

HSP 1 Score: 50.8322 bits (120), Expect = 1.972e-7
Identity = 21/42 (50.00%), Postives = 33/42 (78.57%), Query Frame = 3

Query:   18 TSARASLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            T +  S S   ++F CTVG+KPSKAKY+L++ S+++++LQGL
Sbjct:  798 TLSAQSSSMSTEIFACTVGRKPSKAKYFLDEVSDVVKLLQGL 839

[Close]

BLAST of EX570107 vs. TAIR peptide
Match: AT1G70290.1 (| Symbols: ATTPS8, TPS8, ATTPSC | trehalose-6-phosphatase synthase S8 | chr1:26471286-26474078 REVERSE LENGTH=856)

HSP 1 Score: 49.6766 bits (117), Expect = 4.393e-7
Identity = 21/47 (44.68%), Postives = 35/47 (74.47%), Query Frame = 3

Query:    3 MFGVITSARASLSPIADVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            +   +T+    + P  +VF CTVG+KPSKAKY+L+D +++L++L+GL
Sbjct:  790 ILSTVTNPELLVQP--EVFACTVGRKPSKAKYFLDDEADVLKLLRGL 834

[Close]

BLAST of EX570107 vs. TAIR peptide
Match: AT1G23870.1 (| Symbols: ATTPS9, TPS9 | trehalose-phosphatase/synthase 9 | chr1:8432695-8435506 FORWARD LENGTH=867)

HSP 1 Score: 49.2914 bits (116), Expect = 5.737e-7
Identity = 19/31 (61.29%), Postives = 28/31 (90.32%), Query Frame = 3

Query:   51 DVFPCTVGQKPSKAKYYLEDTSEILRMLQGL 143
            ++F CTVG+KPSKAKY+L+D S++L++L GL
Sbjct:  809 EIFACTVGRKPSKAKYFLDDVSDVLKLLGGL 839

The following BLAST results are available for this feature:

BLAST of EX570107 vs. TrEMBL
BLAST of EX570107 vs. SwissProt
BLAST of EX570107 vs. TAIR peptide

BLAST of EX570107 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SNT9_RICCO	7.830e-14	85.71	Trehalose-6-phosphate synthase, putative OS=Ricinu...	[more]
B9PB85_POPTR	3.886e-13	83.67	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9GZA1_POPTR	5.075e-13	81.63	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q5D6D9_GINBI	5.611e-12	79.59	Trehalose-6-phosphate synthase OS=Ginkgo biloba PE...	[more]
D7TTU3_VITVI	1.250e-11	75.51	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5C0J5_VITVI	1.250e-11	75.51	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q56YF4_ARATH	6.204e-11	69.39	Trehalose-6-phosphate synthase like protein OS=Ara...	[more]
D7MDE9_ARALY	8.103e-11	67.35	ATTPS5 OS=Arabidopsis lyrata subsp. lyrata GN=ARAL...	[more]
Q2A970_BRAOL	1.058e-10	67.35	Trehalose-6-phosphate synthase, putative OS=Brassi...	[more]
B9SNF1_RICCO	6.859e-10	67.35	Trehalose-6-phosphate synthase, putative OS=Ricinu...	[more]

BLAST of EX570107 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
TPS5_ARATH	3.127e-12	69.39	Alpha,alpha-trehalose-phosphate synthase [UDP-form...	[more]
TPS6_ARATH	6.520e-10	61.22	Alpha,alpha-trehalose-phosphate synthase [UDP-form...	[more]

BLAST of EX570107 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT4G17770.1	2.405e-13	69.39	\| Symbols: ATTPS5, TPS5 \| trehalose phosphatase/sy...	[more]
AT1G68020.2	5.014e-11	61.22	\| Symbols: ATTPS6, TPS6 \| UDP-Glycosyltransferase ...	[more]
AT2G18700.1	1.510e-7	63.64	\| Symbols: ATTPS11, TPS11, ATTPSB \| trehalose phos...	[more]
AT1G06410.1	1.510e-7	57.14	\| Symbols: ATTPS7, TPS7, ATTPSA \| trehalose-phosph...	[more]
AT1G60140.1	1.972e-7	50.00	\| Symbols: ATTPS10, TPS10 \| trehalose phosphate sy...	[more]
AT1G70290.1	4.393e-7	44.68	\| Symbols: ATTPS8, TPS8, ATTPSC \| trehalose-6-phos...	[more]
AT1G23870.1	5.737e-7	61.29	\| Symbols: ATTPS9, TPS9 \| trehalose-phosphatase/sy...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.1000	no description	coord: 17..57 score: 1.4
None	No IPR available	SEG	seg	seg	coord: 48..60 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EX570107 ID=EX570107; Name=EX570107; organism=Pisum sativum; type=EST; length=351bp
ACATGTTTGGAGTAATAACAAGTGCAAGGGCATCGCTATCGCCTATTGCT
GACGTGTTTCCTTGTACTGTTGGTCAGAAACCAAGCAAGGCAAAGTATTA
CTTGGAAGACACAAGTGAGATTTTAAGGATGTTGCAGGGACTTGCCAATG
CTTCTGAGCAAAGTGCTGCTAGAAGTTCTTCACAGTTTCCTCATCATTAA
GCTTTTTCTAATATATGTAGGAAACATATACTAATATCATTGGCTAAATT
GTAGTATGTGTGAATACTTGGTTGGTTTTTTGTGCTGTATTTTTAGATCT
GTTTTTATTTATAATCCCAACATTGTTGCTACTATAGATTGTATCTATGT
T

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