AM161886

EST Overview


Name	AM161886
Unique Name	AM161886
Type	EST
Organism	Pisum sativum (pea)
Sequence length	396

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of AM161886 vs. TrEMBL
Match: B9SI39_RICCO (Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial, putative OS=Ricinus communis GN=RCOM_0612620 PE=4 SV=1)

HSP 1 Score: 235.343 bits (599), Expect = 1.454e-60
Identity = 118/131 (90.08%), Postives = 123/131 (93.89%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            GV EHAIFLREVHHAQEIRRKLLLNLMLSDVPGI EEEK RLLHCVVVGGGPTGVEFSGELSDFI +DVRQRY HVKDYI VTLIEANEILSSFDDRLR YATKQLTKSGV+LVRGIVKDVK QK++L+DG
Sbjct:  188 GVKEHAIFLREVHHAQEIRRKLLLNLMLSDVPGISEEEKSRLLHCVVVGGGPTGVEFSGELSDFITRDVRQRYAHVKDYIHVTLIEANEILSSFDDRLRHYATKQLTKSGVQLVRGIVKDVKPQKLVLSDG 318

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BLAST of AM161886 vs. TrEMBL
Match: C3VXF8_ACTDE (Putative mitochondrial type II NAD(P)H dehydrogenase (Fragment) OS=Actinidia deliciosa GN=NDA1 PE=2 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 1.608e-59
Identity = 116/132 (87.88%), Postives = 124/132 (93.94%), Query Frame = 1

Query:    1 QGVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            +GVNEHAIFLREV+HAQEIRRKLLLNLMLSDVPG+ EEEK RLLHCVVVGGGPTGVEFSGELSDFI++DV QRY HVK+YI VTLIEANEILSSFDDRLR YATKQL KSGVRLVRGIVKDV+ QKIIL+DG
Sbjct:   75 KGVNEHAIFLREVYHAQEIRRKLLLNLMLSDVPGVTEEEKSRLLHCVVVGGGPTGVEFSGELSDFIIRDVHQRYAHVKNYIHVTLIEANEILSSFDDRLRQYATKQLVKSGVRLVRGIVKDVQPQKIILSDG 206

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BLAST of AM161886 vs. TrEMBL
Match: D7SJW3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024722001 PE=4 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 7.979e-59
Identity = 116/131 (88.55%), Postives = 120/131 (91.60%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            GV EHA FLREVHHAQEIRRKLLLNLMLSDVPGI E EKQRLLHCVVVGGGPTGVEFSGELSDFI +DV QRY HVK+YI VTLIEANEILSSFDDRLR YAT+QLTKSGVRLVRGIVKDVK  KIILN+G
Sbjct:  189 GVEEHAFFLREVHHAQEIRRKLLLNLMLSDVPGISEAEKQRLLHCVVVGGGPTGVEFSGELSDFITRDVHQRYAHVKNYIHVTLIEANEILSSFDDRLRHYATRQLTKSGVRLVRGIVKDVKVDKIILNNG 319

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BLAST of AM161886 vs. TrEMBL
Match: Q9ST63_SOLTU (Putative internal rotenone-insensitive NADH dehydrogenase OS=Solanum tuberosum GN=nda1 PE=2 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.777e-58
Identity = 113/132 (85.61%), Postives = 122/132 (92.42%), Query Frame = 1

Query:    1 QGVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            +GVNEHA FLREVHHAQEIRRKLLLNLMLSDVPG+ EEEK+RLLHCVVVGGGPTGVEFSGELSDFI+KDV QRY HVKDYI VTLIEANEILSSFDDRLR+YAT QLTKSGVRLVRG+V+ V+   IIL+DG
Sbjct:  185 KGVNEHATFLREVHHAQEIRRKLLLNLMLSDVPGVSEEEKRRLLHCVVVGGGPTGVEFSGELSDFILKDVHQRYAHVKDYIHVTLIEANEILSSFDDRLRVYATNQLTKSGVRLVRGLVQHVQPDNIILSDG 316

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BLAST of AM161886 vs. TrEMBL
Match: B9HP27_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_203252 PE=4 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.777e-58
Identity = 115/131 (87.79%), Postives = 120/131 (91.60%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            GV EHAIFLREV HAQEIRRKLLLNLMLSD+PG+ EEEK RLLHCVVVGGGPTGVEFSGELSDFI+KDVRQ Y+HVKDYI VTLIEANEILSSFDD LR YATKQLTKSGV LVRGIVKDVK QK+IL DG
Sbjct:  142 GVKEHAIFLREVRHAQEIRRKLLLNLMLSDMPGLSEEEKSRLLHCVVVGGGPTGVEFSGELSDFILKDVRQTYSHVKDYIHVTLIEANEILSSFDDSLRRYATKQLTKSGVHLVRGIVKDVKPQKLILTDG 272

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BLAST of AM161886 vs. TrEMBL
Match: A9PHN0_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.777e-58
Identity = 115/131 (87.79%), Postives = 120/131 (91.60%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            GV EHAIFLREV HAQEIRRKLLLNLMLSD+PG+ EEEK RLLHCVVVGGGPTGVEFSGELSDFI+KDVRQ Y+HVKDYI VTLIEANEILSSFDD LR YATKQLTKSGV LVRGIVKDVK QK+IL DG
Sbjct:  178 GVKEHAIFLREVRHAQEIRRKLLLNLMLSDMPGLSEEEKSRLLHCVVVGGGPTGVEFSGELSDFILKDVRQTYSHVKDYIHVTLIEANEILSSFDDSLRRYATKQLTKSGVHLVRGIVKDVKPQKLILTDG 308

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BLAST of AM161886 vs. TrEMBL
Match: Q5QLT6_ORYSJ (Os01g0830100 protein OS=Oryza sativa subsp. japonica GN=B1088C09.41-1 PE=2 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 6.754e-58
Identity = 113/132 (85.61%), Postives = 124/132 (93.94%), Query Frame = 1

Query:    1 QGVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            +GV +HAIFLREVHHAQEIRRKLLLNLMLSDVPGI EEEK+RLLHCVVVGGGPTGVEFSGELSDFI++DV+QRY+HVKDYI VTLIEANEILSSFD RLR YAT QLTKSGVRLVRGIVKDV+  K+IL++G
Sbjct:  188 RGVTDHAIFLREVHHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGGGPTGVEFSGELSDFIIRDVKQRYSHVKDYIHVTLIEANEILSSFDVRLRQYATNQLTKSGVRLVRGIVKDVQPNKLILDNG 319

[Close]

BLAST of AM161886 vs. TrEMBL
Match: B9EU97_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_03953 PE=4 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 6.754e-58
Identity = 113/132 (85.61%), Postives = 124/132 (93.94%), Query Frame = 1

Query:    1 QGVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            +GV +HAIFLREVHHAQEIRRKLLLNLMLSDVPGI EEEK+RLLHCVVVGGGPTGVEFSGELSDFI++DV+QRY+HVKDYI VTLIEANEILSSFD RLR YAT QLTKSGVRLVRGIVKDV+  K+IL++G
Sbjct:  188 RGVTDHAIFLREVHHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGGGPTGVEFSGELSDFIIRDVKQRYSHVKDYIHVTLIEANEILSSFDVRLRQYATNQLTKSGVRLVRGIVKDVQPNKLILDNG 319

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BLAST of AM161886 vs. TrEMBL
Match: B8ABM9_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_04297 PE=4 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 6.754e-58
Identity = 113/132 (85.61%), Postives = 124/132 (93.94%), Query Frame = 1

Query:    1 QGVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            +GV +HAIFLREVHHAQEIRRKLLLNLMLSDVPGI EEEK+RLLHCVVVGGGPTGVEFSGELSDFI++DV+QRY+HVKDYI VTLIEANEILSSFD RLR YAT QLTKSGVRLVRGIVKDV+  K+IL++G
Sbjct:  188 RGVTDHAIFLREVHHAQEIRRKLLLNLMLSDVPGISEEEKRRLLHCVVVGGGPTGVEFSGELSDFIIRDVKQRYSHVKDYIHVTLIEANEILSSFDVRLRQYATNQLTKSGVRLVRGIVKDVQPNKLILDNG 319

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BLAST of AM161886 vs. TrEMBL
Match: D7KGZ8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_887930 PE=4 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 9.753e-57
Identity = 113/131 (86.26%), Postives = 122/131 (93.13%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            GV E+AIFLREVHHAQEIRRKLLLNLMLS+VPGI E+EK+RLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRY HVKD IRVTLIEA +ILSSFDDRLR YA KQL KSGV+LVRGIVK+VK QK+IL+DG
Sbjct:  199 GVLENAIFLREVHHAQEIRRKLLLNLMLSEVPGISEDEKKRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYAHVKDDIRVTLIEARDILSSFDDRLRQYAIKQLNKSGVKLVRGIVKEVKPQKLILDDG 329

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BLAST of AM161886 vs. SwissProt
Match: NDH_DICDI (Probable NADH dehydrogenase OS=Dictyostelium discoideum GN=DDB_G0270104 PE=3 SV=2)

HSP 1 Score: 96.2857 bits (238), Expect = 5.178e-20
Identity = 53/135 (39.26%), Postives = 89/135 (65.93%), Query Frame = 1

Query:    1 QGVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVK-DYIRVTLIEAN-EILSSFDDRLRLYATKQLTKSGVRL-VRGIVKDVKAQKIILNDG 396
            +GV E+A FL+E+HHA+EIR+K++     + +P +  EE++RLL  V+VGGG TG+EF+ EL+DF  +D+ + +  V  + +++ L+EA+ +ILS+FD +L   A      SG+ +     VK+V    +IL++G
Sbjct:  156 KGVEENANFLKELHHAREIRQKIIECFERASLPDVSTEERERLLSFVIVGGGATGIEFTSELNDFFSEDLSRLFPFVPVNEVKIILLEASGKILSTFDQKLVKKALINFRNSGIDVRTHSSVKEVLKDYVILDNG 290

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BLAST of AM161886 vs. SwissProt
Match: NDH1_SCHPO (Probable NADH-ubiquinone oxidoreductase C3A11.07, mitochondrial OS=Schizosaccharomyces pombe GN=SPAC3A11.07 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.267e-18
Identity = 49/128 (38.28%), Postives = 76/128 (59.38%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEA-NEILSSFDDRLRLYATKQLTKSGVRL-VRGIVKDVKAQKI 381
            G+ E+  FL+E+  AQ+IR ++L  L  +    +P E ++R +H VVVGGGPTG+EF+GE++DFI  D++  Y  + D   VTL+EA   +L  F  +LR Y       S +++     +K V A+ I
Sbjct:  213 GIAEYGCFLKEIWDAQKIRARILHCLEQAQFKDLPAETRRRYVHTVVVGGGPTGMEFAGEMADFIEDDLKSWYPELADDFAVTLVEALPSVLPMFSAKLRDYTQSLFDSSHIKIRTNTALKKVTAENI 340

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BLAST of AM161886 vs. SwissProt
Match: NDH1_YEAST (External NADH-ubiquinone oxidoreductase 1, mitochondrial OS=Saccharomyces cerevisiae GN=NDE1 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 3.473e-16
Identity = 53/133 (39.85%), Postives = 79/133 (59.40%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEA-NEILSSFDDRLRLYATKQLTKSGVRL-VRGIVKDVKAQKIILNDG 396
            GV E++ FL+E+  AQEIR K++ ++  +      + E+ RLL  VVVGGGPTGVEF+ EL D++ +D+R+    +   I+VTL+EA   IL+ FD  L  YA     +  + L ++ +VK V A  I    G
Sbjct:  233 GVYEYSSFLKEISDAQEIRLKIMSSIEKAASLSPKDPERARLLSFVVVGGGPTGVEFAAELRDYVDQDLRKWMPELSKEIKVTLVEALPNILNMFDKYLVDYAQDLFKEEKIDLRLKTMVKKVDATTITAKTG 365

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BLAST of AM161886 vs. SwissProt
Match: NDI1_YEAST (Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial OS=Saccharomyces cerevisiae GN=NDI1 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.251e-15
Identity = 45/129 (34.88%), Postives = 82/129 (63.57%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEI-LSSFDDRLRLYATKQLTKSGVRL-VRGIVKDVKAQKII 384
            GV ++  FL+E+ ++ EIRR    NL  +++    + E++RLL  VVVGGGPTGVE +GEL D++ +D+R+    + + +++ L+EA  I L+ F+ +L  YA   L  + +++ +R  V  V+ ++++
Sbjct:  187 GVTDYGHFLKEIPNSLEIRRTFAANLEKANLLPKGDPERRRLLSIVVVGGGPTGVEAAGELQDYVHQDLRKFLPALAEEVQIHLVEALPIVLNMFEKKLSSYAQSHLENTSIKVHLRTAVAKVEEKQLL 315

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BLAST of AM161886 vs. SwissProt
Match: NDH2_SCHPO (Probable NADH-ubiquinone oxidoreductase C947.15c, mitochondrial OS=Schizosaccharomyces pombe GN=SPBC947.15c PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.550e-15
Identity = 47/132 (35.61%), Postives = 74/132 (56.06%), Query Frame = 1

Query:    1 QGVNEHAIFLREVHHAQEIRRKLLLNL-MLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANE-ILSSFDDRLRLYATKQLTKSGVRLV-RGIVKDVKAQKIIL 387
            QGV +H  FL+E   A+++  ++   L  +     +  EE+ RLLH  VVGGGPTG+EF+ E+ DFI  DV+  +  ++  I VTLIEA   +L  F   L  Y         ++++ + +VKDV  + +I+
Sbjct:  211 QGVRDHGCFLKEAGDAKKVFNRIFEILEQVRFNKDLSPEERARLLHITVVGGGPTGMEFAAEMQDFIDNDVKDMFPELQKDIHVTLIEAAPGVLPMFTKSLITYTENLFKNLNIKIMTKTVVKDVNEKNLIV 342

[Close]

BLAST of AM161886 vs. SwissProt
Match: NDH2_YEAST (External NADH-ubiquinone oxidoreductase 2, mitochondrial OS=Saccharomyces cerevisiae GN=NDE2 PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.459e-14
Identity = 47/114 (41.23%), Postives = 68/114 (59.65%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEA-NEILSSFDDRLRLYATKQLTKSGVRL 342
            GV  +A FL+E+  AQ IR KL+  +  +    + + E++RLL  VVVGGGPTGVEF+ EL D+I +D+R+    +   ++V LIEA   IL+ FD  L  YA     +  + L
Sbjct:  218 GVYGNANFLKEIEDAQNIRMKLMKTIEQASSFPVNDPERKRLLTFVVVGGGPTGVEFAAELQDYINQDLRKWMPDLSKEMKVILIEALPNILNMFDKTLIKYAEDLFARDEIDL 331

[Close]

BLAST of AM161886 vs. SwissProt
Match: DHNA_ECOLI (NADH dehydrogenase OS=Escherichia coli (strain K12) GN=ndh PE=1 SV=2)

HSP 1 Score: 56.225 bits (134), Expect = 5.938e-8
Identity = 41/116 (35.34%), Postives = 62/116 (53.45%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHV-KDYIRVTLIEANE-ILSSFDDRLRLYATKQLTKSGVRLV 345
            GV E+ IFL   H A+   +++L NL L     +    K   ++  +VGGG TGVE S EL + + +     Y  +  + + VTL+EA E IL +   R+   A  +LTK GVR++
Sbjct:  133 GVKENCIFLDNPHQARRFHQEML-NLFLKYSANLGANGK---VNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLGVRVL 244

[Close]

BLAST of AM161886 vs. TAIR peptide
Match: AT1G07180.1 (| Symbols: ATNDI1, NDA1 | alternative NAD(P)H dehydrogenase 1 | chr1:2204414-2206773 FORWARD LENGTH=510)

HSP 1 Score: 222.246 bits (565), Expect = 6.775e-59
Identity = 113/131 (86.26%), Postives = 123/131 (93.89%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            GV E+AIFLREVHHAQEIRRKLLLNLMLS+VPGI E+EK+RLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRY+HVKD IRVTLIEA +ILSSFDDRLR YA KQL KSGV+LVRGIVK+VK QK+IL+DG
Sbjct:  200 GVLENAIFLREVHHAQEIRRKLLLNLMLSEVPGIGEDEKKRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYSHVKDDIRVTLIEARDILSSFDDRLRHYAIKQLNKSGVKLVRGIVKEVKPQKLILDDG 330

[Close]

BLAST of AM161886 vs. TAIR peptide
Match: AT2G29990.1 (| Symbols: NDA2 | alternative NAD(P)H dehydrogenase 2 | chr2:12793562-12795913 REVERSE LENGTH=508)

HSP 1 Score: 221.09 bits (562), Expect = 1.509e-58
Identity = 112/131 (85.50%), Postives = 120/131 (91.60%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANEILSSFDDRLRLYATKQLTKSGVRLVRGIVKDVKAQKIILNDG 396
            GV E+AIFLREVHHAQEIRRKLLLNLMLSD PGI +EEK+RLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRY HVKD I VTLIEA +ILSSFDDRLR YA KQL KSGVR VRGIVKDV++QK+IL+DG
Sbjct:  198 GVMENAIFLREVHHAQEIRRKLLLNLMLSDTPGISKEEKRRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYAHVKDDIHVTLIEARDILSSFDDRLRRYAIKQLNKSGVRFVRGIVKDVQSQKLILDDG 328

[Close]

BLAST of AM161886 vs. TAIR peptide
Match: AT4G28220.1 (| Symbols: NDB1 | NAD(P)H dehydrogenase B1 | chr4:13993078-13995651 FORWARD LENGTH=571)

HSP 1 Score: 105.916 bits (263), Expect = 7.076e-24
Identity = 51/118 (43.22%), Postives = 85/118 (72.03%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANE-ILSSFDDRLRLYATKQLTKSGVRLVRGI 354
            GV E+  FL+EV  AQ IRR ++     + +PG+ EE+++R LH V+VGGGPTGVEF+ EL DFI++D+ + Y  VK+ +++TLI++ + IL++FD+R+  +A ++ T+ G+ +  G+
Sbjct:  173 GVLENCHFLKEVEDAQRIRRGVIDCFEKAILPGLTEEQRRRKLHFVIVGGGPTGVEFAAELHDFIIEDITKIYPSVKELVKITLIQSGDHILNTFDERISSFAEQKFTRDGIDVQTGM 290

[Close]

BLAST of AM161886 vs. TAIR peptide
Match: AT2G20800.1 (| Symbols: NDB4 | NAD(P)H dehydrogenase B4 | chr2:8953252-8955699 REVERSE LENGTH=582)

HSP 1 Score: 105.916 bits (263), Expect = 7.076e-24
Identity = 55/128 (42.97%), Postives = 85/128 (66.41%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIEANE-ILSSFDDRLRLYATKQLTKSGVRLVRG-IVKDVKAQKI 381
            GV EHA FL+E   A  IR  ++     + +P + EEE++++LH VVVGGGPTGVEFS EL DF+++DV + Y  V+++ ++TL+EA + IL+ FD R+  +A ++  + G+ L  G +V  V A +I
Sbjct:  185 GVEEHAYFLKEAEDALNIRHSVIDCFERASLPNLTEEERKKILHFVVVGGGPTGVEFSAELHDFLVQDVAKIYPKVQEFTKITLLEAGDHILNMFDKRITAFAEEKFQRDGIDLKTGSMVVGVTADEI 312

[Close]

BLAST of AM161886 vs. TAIR peptide
Match: AT4G21490.1 (| Symbols: NDB3 | NAD(P)H dehydrogenase B3 | chr4:11436495-11439326 REVERSE LENGTH=580)

HSP 1 Score: 102.449 bits (254), Expect = 7.823e-23
Identity = 50/117 (42.74%), Postives = 81/117 (69.23%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIE-ANEILSSFDDRLRLYATKQLTKSGVRLVRG 351
            GV E+  FL+EV  AQ IR  ++ +   + +PG+ E+E++R+LH VVVGGGPTGVEF+ EL DF+ +D+ + Y   K+ +++TL+E A+ IL+ FD R+  +A ++ T+ G+ +  G
Sbjct:  179 GVEENCHFLKEVEDAQRIRSTVIDSFEKASLPGLNEQERKRMLHFVVVGGGPTGVEFASELHDFVNEDLVKLYPKAKNLVQITLLEAADHILTMFDKRITEFAEEKFTRDGIDVKLG 295

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BLAST of AM161886 vs. TAIR peptide
Match: AT4G05020.2 (| Symbols: NDB2 | NAD(P)H dehydrogenase B2 | chr4:2572752-2576222 FORWARD LENGTH=619)

HSP 1 Score: 100.523 bits (249), Expect = 2.973e-22
Identity = 52/131 (39.69%), Postives = 85/131 (64.89%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIE-ANEILSSFDDRLRLYATKQLTKSGVRLVRGIV------KDVKAQ 375
            GV E+  FL+EV  AQ IR+ ++ +   + +P + +EE++R+LH VVVGGGPTGVEF+ EL DF+ +D+   Y   K  +R+TL+E A+ IL+ FD R+  +A ++ ++ G+ +  G +      KD+ A+
Sbjct:  181 GVEENCHFLKEVEDAQRIRKTVIDSFEKASLPELSDEERKRILHFVVVGGGPTGVEFAAELHDFVTEDLVSLYPRAKGSVRITLLEAADHILTMFDKRITEFAEEKFSRDGIDVKLGSMVTKVNEKDISAK 311

[Close]

BLAST of AM161886 vs. TAIR peptide
Match: AT4G05020.1 (| Symbols: NDB2 | NAD(P)H dehydrogenase B2 | chr4:2572752-2576222 FORWARD LENGTH=582)

HSP 1 Score: 100.523 bits (249), Expect = 2.973e-22
Identity = 52/131 (39.69%), Postives = 85/131 (64.89%), Query Frame = 1

Query:    4 GVNEHAIFLREVHHAQEIRRKLLLNLMLSDVPGIPEEEKQRLLHCVVVGGGPTGVEFSGELSDFIMKDVRQRYTHVKDYIRVTLIE-ANEILSSFDDRLRLYATKQLTKSGVRLVRGIV------KDVKAQ 375
            GV E+  FL+EV  AQ IR+ ++ +   + +P + +EE++R+LH VVVGGGPTGVEF+ EL DF+ +D+   Y   K  +R+TL+E A+ IL+ FD R+  +A ++ ++ G+ +  G +      KD+ A+
Sbjct:  181 GVEENCHFLKEVEDAQRIRKTVIDSFEKASLPELSDEERKRILHFVVVGGGPTGVEFAAELHDFVTEDLVSLYPRAKGSVRITLLEAADHILTMFDKRITEFAEEKFSRDGIDVKLGSMVTKVNEKDISAK 311

The following BLAST results are available for this feature:

BLAST of AM161886 vs. TrEMBL
BLAST of AM161886 vs. SwissProt
BLAST of AM161886 vs. TAIR peptide

BLAST of AM161886 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SI39_RICCO	1.454e-60	90.08	Rotenone-insensitive NADH-ubiquinone oxidoreductas...	[more]
C3VXF8_ACTDE	1.608e-59	87.88	Putative mitochondrial type II NAD(P)H dehydrogena...	[more]
D7SJW3_VITVI	7.979e-59	88.55	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q9ST63_SOLTU	1.777e-58	85.61	Putative internal rotenone-insensitive NADH dehydr...	[more]
B9HP27_POPTR	1.777e-58	87.79	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
A9PHN0_POPTR	1.777e-58	87.79	Putative uncharacterized protein OS=Populus tricho...	[more]
Q5QLT6_ORYSJ	6.754e-58	85.61	Os01g0830100 protein OS=Oryza sativa subsp. japoni...	[more]
B9EU97_ORYSJ	6.754e-58	85.61	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8ABM9_ORYSI	6.754e-58	85.61	Putative uncharacterized protein OS=Oryza sativa s...	[more]
D7KGZ8_ARALY	9.753e-57	86.26	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of AM161886 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
NDH_DICDI	5.178e-20	39.26	Probable NADH dehydrogenase OS=Dictyostelium disco...	[more]
NDH1_SCHPO	8.267e-18	38.28	Probable NADH-ubiquinone oxidoreductase C3A11.07, ...	[more]
NDH1_YEAST	3.473e-16	39.85	External NADH-ubiquinone oxidoreductase 1, mitocho...	[more]
NDI1_YEAST	2.251e-15	34.88	Rotenone-insensitive NADH-ubiquinone oxidoreductas...	[more]
NDH2_SCHPO	6.550e-15	35.61	Probable NADH-ubiquinone oxidoreductase C947.15c, ...	[more]
NDH2_YEAST	1.459e-14	41.23	External NADH-ubiquinone oxidoreductase 2, mitocho...	[more]
DHNA_ECOLI	5.938e-8	35.34	NADH dehydrogenase OS=Escherichia coli (strain K12...	[more]

BLAST of AM161886 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT1G07180.1	6.775e-59	86.26	\| Symbols: ATNDI1, NDA1 \| alternative NAD(P)H deh...	[more]
AT2G29990.1	1.509e-58	85.50	\| Symbols: NDA2 \| alternative NAD(P)H dehydrogenas...	[more]
AT4G28220.1	7.076e-24	43.22	\| Symbols: NDB1 \| NAD(P)H dehydrogenase B1 \| chr4:...	[more]
AT2G20800.1	7.076e-24	42.97	\| Symbols: NDB4 \| NAD(P)H dehydrogenase B4 \| chr2:...	[more]
AT4G21490.1	7.823e-23	42.74	\| Symbols: NDB3 \| NAD(P)H dehydrogenase B3 \| chr4:...	[more]
AT4G05020.2	2.973e-22	39.69	\| Symbols: NDB2 \| NAD(P)H dehydrogenase B2 \| chr4:...	[more]
AT4G05020.1	2.973e-22	39.69	\| Symbols: NDB2 \| NAD(P)H dehydrogenase B2 \| chr4:...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001327	Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region	PFAM	PF00070	Pyr_redox	coord: 45..116 score: 4.7
None	No IPR available	GENE3D	3.50.50.60	no description	coord: 10..129 score: 2.5
None	No IPR available	PANTHER	PTHR22915	NADH DEHYDROGENASE-RELATED	coord: 1..131 score: 2.1
None	No IPR available	PANTHER	PTHR22915:SF4	NADH DEHYDROGENASE-RELATED	coord: 1..131 score: 2.1
None	No IPR available	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 3..132 score: 8.8

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM161886 ID=AM161886; Name=AM161886; organism=Pisum sativum; type=EST; length=396bp
CAAGGAGTCAATGAACATGCCATTTTTCTTCGCGAAGTTCACCATGCACA
GGAAATTCGTAGGAAGTTGCTCCTAAACTTGATGCTATCTGATGTTCCAG
GAATTCCAGAAGAGGAAAAGCAAAGGCTTTTGCACTGTGTTGTTGTTGGG
GGTGGTCCTACTGGAGTTGAATTCAGTGGTGAACTTAGTGATTTTATCAT
GAAGGATGTGCGCCAAAGATATACACATGTGAAGGATTACATCCGTGTTA
CTTTAATTGAGGCAAATGAGATATTATCTTCTTTTGATGACCGACTCAGG
CTCTATGCTACCAAGCAGTTGACAAAGTCAGGAGTTCGTCTTGTTCGTGG
CATTGTGAAAGATGTTAAAGCTCAGAAGATTATCCTAAATGACGGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0050660	flavin adenine dinucleotide binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR001327	Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain

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