AM162007

EST Overview
NameAM162007
Unique NameAM162007
TypeEST
OrganismPisum sativum (pea)
Sequence length420
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
Homology
[Close]
BLAST of AM162007 vs. TrEMBL
Match: B7FK62_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.445e-15
Identity = 37/48 (77.08%), Postives = 41/48 (85.42%), Query Frame = -2 
Query:  195 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYD 338
            ANEIAVELFV  K ++  DIFK+ ELTCNEHQKELVTSP+LEEI HYD
Sbjct:  274 ANEIAVELFVAEK-ISYLDIFKIAELTCNEHQKELVTSPTLEEIFHYD 320

HSP 2 Score: 33.113 bits (74), Expect = 1.445e-15
Identity = 16/23 (69.57%), Postives = 18/23 (78.26%), Query Frame = -3 
Query:  149 LKKFFTMI*WARQYAAGLQKALS 217
            L++ F    WARQYAAGLQKALS
Sbjct:  313 LEEIFHYDQWARQYAAGLQKALS 335
[Close]
BLAST of AM162007 vs. TrEMBL
Match: Q6EJD0_PUELO (1-deoxy-D-xylulose 5-phosphate reductoisomerase OS=Pueraria lobata GN=Dxr PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.846e-12
Identity = 35/48 (72.92%), Postives = 40/48 (83.33%), Query Frame = -2 
Query:  195 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYD 338
            ANE AVE+FV  K ++  DIFKVVELTC EHQKELV +PSLEEI+HYD
Sbjct:  403 ANEKAVEMFVEEK-ISYLDIFKVVELTCQEHQKELVVAPSLEEIIHYD 449

HSP 2 Score: 27.7202 bits (60), Expect = 2.846e-12
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = -3 
Query:  149 WARQYAAGLQKALS 190
            WARQYAA LQKA S
Sbjct:  451 WARQYAASLQKASS 464
[Close]
BLAST of AM162007 vs. TrEMBL
Match: Q703F3_LINUS (1-deoxy-D-xylulose 5-phosphate reductoisomerase OS=Linum usitatissimum GN=dxr PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.877e-11
Identity = 36/52 (69.23%), Postives = 43/52 (82.69%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVELF+  K +A  DIFK+VELTC +H++ELVTSPSLEEI+HYDL  K
Sbjct:  409 ANEKAVELFIDEK-IAYLDIFKIVELTCAKHREELVTSPSLEEIIHYDLWAK 459

HSP 2 Score: 22.3274 bits (46), Expect = 3.877e-11
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = -3 
Query:  155 WARQYAAGLQKA 190
            WA+ YAA LQ+A
Sbjct:  457 WAKDYAASLQQA 468
[Close]
BLAST of AM162007 vs. TrEMBL
Match: Q1L7P3_9GENT (1-deoxy-D-xylulose 5-phosphate reductoisomerase OS=Rauvolfia verticillata PE=2 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 5.035e-11
Identity = 36/52 (69.23%), Postives = 42/52 (80.77%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVELF+  K ++  DIFKVVELTC +HQ ELVTSPSLEEI+HYDL  +
Sbjct:  407 ANEKAVELFINEK-ISYLDIFKVVELTCEKHQAELVTSPSLEEIIHYDLWSR 457

HSP 2 Score: 22.3274 bits (46), Expect = 5.035e-11
Identity = 8/13 (61.54%), Postives = 10/13 (76.92%), Query Frame = -3 
Query:  152 WARQYAAGLQKAL 190
            W+R YAAG+Q  L
Sbjct:  455 WSRDYAAGVQGTL 467
[Close]
BLAST of AM162007 vs. TrEMBL
Match: Q9FXZ7_MAIZE (1-deoxy-D-xylulose 5-phosphate reductoisomerase OS=Zea mays GN=dxr PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 6.537e-11
Identity = 36/52 (69.23%), Postives = 42/52 (80.77%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVELF+  K ++  DIFKVVELTCN H+ ELVTSPSLEEI+HYDL  +
Sbjct:  405 ANEKAVELFIDEK-ISYLDIFKVVELTCNAHRNELVTSPSLEEIVHYDLWAR 455

HSP 2 Score: 21.9422 bits (45), Expect = 6.537e-11
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = -3 
Query:  161 WARQYAAGLQ 190
            WAR+YAA LQ
Sbjct:  453 WARRYAASLQ 462
[Close]
BLAST of AM162007 vs. TrEMBL
Match: Q0MW94_TOBAC (1-deoxy-D-xylulose-5-phosphate reductoisomerase OS=Nicotiana tabacum GN=DXR PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.410e-10
Identity = 33/52 (63.46%), Postives = 43/52 (82.69%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVELF+  + ++  DIFK+VELTC +H++ELVTSPSLEEI+HYDL  +
Sbjct:  406 ANEKAVELFIS-ERISYLDIFKIVELTCAKHREELVTSPSLEEIIHYDLWAR 456

HSP 2 Score: 22.3274 bits (46), Expect = 2.410e-10
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = -3 
Query:  146 WARQYAAGLQKALSF 190
            WAR YAA L+ +  F
Sbjct:  454 WARDYAASLETSAGF 468
[Close]
BLAST of AM162007 vs. TrEMBL
Match: B9I3A1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_728585 PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.410e-10
Identity = 34/52 (65.38%), Postives = 42/52 (80.77%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVE+F+  K ++  DIFKVVELTC++HQ ELV SPSLEEI+HYDL  +
Sbjct:  404 ANEKAVEMFIDEK-ISYLDIFKVVELTCDKHQAELVVSPSLEEIIHYDLWAR 454

HSP 2 Score: 22.3274 bits (46), Expect = 2.410e-10
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = -3 
Query:  161 WARQYAAGLQ 190
            WAR+YAA LQ
Sbjct:  452 WAREYAANLQ 461
[Close]
BLAST of AM162007 vs. TrEMBL
Match: B3GEM1_POPTR (Putative chloroplast 1-deoxy-D-xylulose 5-phosphate reductoisomerase (Fragment) OS=Populus trichocarpa GN=DXR PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.417e-10
Identity = 34/52 (65.38%), Postives = 42/52 (80.77%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVE+F+  K ++  DIFKVVELTC++HQ ELV SPSLEEI+HYDL  +
Sbjct:  359 ANEKAVEMFIDEK-ISYLDIFKVVELTCDKHQAELVVSPSLEEIIHYDLWAR 409

HSP 2 Score: 22.3274 bits (46), Expect = 2.417e-10
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = -3 
Query:  161 WARQYAAGLQ 190
            WAR+YAA LQ
Sbjct:  407 WAREYAANLQ 416
[Close]
BLAST of AM162007 vs. TrEMBL
Match: Q70KX8_9ROSI (1-deoxy-D-xylulose-5-phosphate reductoisomerase OS=Populus tremula x Populus alba GN=ptxa_dxr PE=2 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 3.128e-10
Identity = 34/52 (65.38%), Postives = 42/52 (80.77%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVE+F+  K ++  DIFKVVELTC++HQ ELV SPSLEEI+HYDL  +
Sbjct:  405 ANEKAVEMFIDEK-ISYLDIFKVVELTCDKHQAELVVSPSLEEIVHYDLWAR 455

HSP 2 Score: 22.3274 bits (46), Expect = 3.128e-10
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = -3 
Query:  161 WARQYAAGLQ 190
            WAR+YAA LQ
Sbjct:  453 WAREYAASLQ 462
[Close]
BLAST of AM162007 vs. TrEMBL
Match: D7SHS1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00007752001 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 4.061e-10
Identity = 35/52 (67.31%), Postives = 42/52 (80.77%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVE+F+  K +   DIFKVVELTC++HQ ELVTSPSLEEI+HYDL  +
Sbjct:  404 ANEKAVEMFIDEK-IGYLDIFKVVELTCDKHQAELVTSPSLEEIVHYDLWAR 454

HSP 2 Score: 20.4014 bits (41), Expect = 4.061e-10
Identity = 8/14 (57.14%), Postives = 9/14 (64.29%), Query Frame = -3 
Query:  149 WARQYAAGLQKALS 190
            WAR YAA L  + S
Sbjct:  452 WARDYAASLHHSSS 465
[Close]
BLAST of AM162007 vs. SwissProt
Match: DXR_ARATH (1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic OS=Arabidopsis thaliana GN=DXR PE=2 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 2.003e-11
Identity = 34/52 (65.38%), Postives = 43/52 (82.69%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVE+F+  K ++  DIFKVVELTC++H+ ELVTSPSLEEI+HYDL  +
Sbjct:  410 ANEKAVEMFIDEK-ISYLDIFKVVELTCDKHRNELVTSPSLEEIVHYDLWAR 460

HSP 2 Score: 21.1718 bits (43), Expect = 2.003e-11
Identity = 7/10 (70.00%), Postives = 9/10 (90.00%), Query Frame = -3 
Query:  161 WARQYAAGLQ 190
            WAR+YAA +Q
Sbjct:  458 WAREYAANVQ 467
[Close]
BLAST of AM162007 vs. SwissProt
Match: DXR_ORYSJ (1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic OS=Oryza sativa subsp. japonica GN=DXR PE=2 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 3.375e-11
Identity = 34/52 (65.38%), Postives = 40/52 (76.92%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVELF+  K +   DIFKVVELTC+ H+ ELVT PSLEEI+HYDL  +
Sbjct:  406 ANEKAVELFIDEK-IGYLDIFKVVELTCDAHRNELVTRPSLEEIIHYDLWAR 456

HSP 2 Score: 22.3274 bits (46), Expect = 3.375e-11
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = -3 
Query:  161 WARQYAAGLQ 190
            WAR+YAA LQ
Sbjct:  454 WAREYAASLQ 463
[Close]
BLAST of AM162007 vs. SwissProt
Match: DXR_MENPI (1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic OS=Mentha piperita GN=DXR PE=2 SV=2)

HSP 1 Score: 60.8474 bits (146), Expect = 2.423e-9
Identity = 32/61 (52.46%), Postives = 41/61 (67.21%), Query Frame = -2 
Query:  156 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGKTICCWFAKS 338
            ANE AVE+F+  K +   DIFKVVELTC++H+ E+  SPSLEEI+HYD   +       KS
Sbjct:  403 ANEKAVEMFIDEK-IGYLDIFKVVELTCDKHRSEMAVSPSLEEIVHYDQWARDYAATVLKS 462
[Close]
BLAST of AM162007 vs. TAIR peptide
Match: AT5G62790.2 (| Symbols: DXR | 1-deoxy-D-xylulose 5-phosphate reductoisomerase | chr5:25214358-25217289 REVERSE LENGTH=497)

HSP 1 Score: 66.6254 bits (161), Expect = 2.427e-12
Identity = 34/52 (65.38%), Postives = 43/52 (82.69%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVE+F+  K ++  DIFKVVELTC++H+ ELVTSPSLEEI+HYDL  +
Sbjct:  430 ANEKAVEMFIDEK-ISYLDIFKVVELTCDKHRNELVTSPSLEEIVHYDLWAR 480

HSP 2 Score: 21.1718 bits (43), Expect = 2.427e-12
Identity = 7/10 (70.00%), Postives = 9/10 (90.00%), Query Frame = -3 
Query:  161 WARQYAAGLQ 190
            WAR+YAA +Q
Sbjct:  478 WAREYAANVQ 487
[Close]
BLAST of AM162007 vs. TAIR peptide
Match: AT5G62790.1 (| Symbols: DXR, PDE129 | 1-deoxy-D-xylulose 5-phosphate reductoisomerase | chr5:25214358-25217292 REVERSE LENGTH=477)

HSP 1 Score: 66.6254 bits (161), Expect = 2.430e-12
Identity = 34/52 (65.38%), Postives = 43/52 (82.69%), Query Frame = -2 
Query:  183 ANEIAVELFVV*KGLAI*DIFKVVELTCNEHQKELVTSPSLEEILHYDLMGK 338
            ANE AVE+F+  K ++  DIFKVVELTC++H+ ELVTSPSLEEI+HYDL  +
Sbjct:  410 ANEKAVEMFIDEK-ISYLDIFKVVELTCDKHRNELVTSPSLEEIVHYDLWAR 460

HSP 2 Score: 21.1718 bits (43), Expect = 2.430e-12
Identity = 7/10 (70.00%), Postives = 9/10 (90.00%), Query Frame = -3 
Query:  161 WARQYAAGLQ 190
            WAR+YAA +Q
Sbjct:  458 WAREYAANVQ 467
The following BLAST results are available for this feature:
BLAST of AM162007 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
B7FK62_MEDTR1.445e-1577.08Putative uncharacterized protein OS=Medicago trunc... [more]
Q6EJD0_PUELO2.846e-1272.921-deoxy-D-xylulose 5-phosphate reductoisomerase OS... [more]
Q703F3_LINUS3.877e-1169.231-deoxy-D-xylulose 5-phosphate reductoisomerase OS... [more]
Q1L7P3_9GENT5.035e-1169.231-deoxy-D-xylulose 5-phosphate reductoisomerase OS... [more]
Q9FXZ7_MAIZE6.537e-1169.231-deoxy-D-xylulose 5-phosphate reductoisomerase OS... [more]
Q0MW94_TOBAC2.410e-1063.461-deoxy-D-xylulose-5-phosphate reductoisomerase OS... [more]
B9I3A1_POPTR2.410e-1065.38Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B3GEM1_POPTR2.417e-1065.38Putative chloroplast 1-deoxy-D-xylulose 5-phosphat... [more]
Q70KX8_9ROSI3.128e-1065.381-deoxy-D-xylulose-5-phosphate reductoisomerase OS... [more]
D7SHS1_VITVI4.061e-1067.31Whole genome shotgun sequence of line PN40024, sca... [more]
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BLAST of AM162007 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 3
Match NameE-valueIdentityDescription
DXR_ARATH2.003e-1165.381-deoxy-D-xylulose 5-phosphate reductoisomerase, c... [more]
DXR_ORYSJ3.375e-1165.381-deoxy-D-xylulose 5-phosphate reductoisomerase, c... [more]
DXR_MENPI2.423e-952.461-deoxy-D-xylulose 5-phosphate reductoisomerase, c... [more]
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BLAST of AM162007 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G62790.22.427e-1265.38| Symbols: DXR | 1-deoxy-D-xylulose 5-phosphate re... [more]
AT5G62790.12.430e-1265.38| Symbols: DXR, PDE129 | 1-deoxy-D-xylulose 5-phos... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM162007 ID=AM162007; Name=AM162007; organism=Pisum sativum; type=EST; length=420bp
ATCTCAAAAGATTACAGCATGCTTTAAGAGTTTCCATTGAAACCCTTTTT
AACACATATATAATATATTGTTACCCTTTACATTTTTATACAATTTATGA
ACATCAACAAAATATGCTATTGTTGATAACAATTAACCACATTCAAAAAC
TCAAAGCTTTTTGCAAACCAGCAGCATATTGTCTTGCCCATTAGATCATA
GTGAAGAATTTCTTCAAGTGAAGGAGATGTTACCAATTCCTTCTGATGTT
CATTGCATGTTAGCTCCACAACCTTGAAAATATCTCAGATAGCTAATCCT
TTCTAGACAACAAACAGTTCTACAGCTATCTCGTTTGCAAGCACTGATAA
CTCCGGTCATGGTGCCTCCGGCTCGTCCAGCAGCAGTAGCAAAGATTCAN
CGGACGGAAACTTCTTGGTG
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