AM162151

EST Overview
NameAM162151
Unique NameAM162151
TypeEST
OrganismPisum sativum (pea)
Sequence length420
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Pisum sativum unigene v12010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide2011-02-03
Homology
[Close]
BLAST of AM162151 vs. TrEMBL
Match: D7SJ29_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00008332001 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 6.275e-12
Identity = 26/42 (61.90%), Postives = 35/42 (83.33%), Query Frame = -2 
Query:  243 KNGVSDNQLANSLLQAADNWLRLVQVNHPDYKFFVSHGTYHK 368
            +NG  ++Q  NSL+QAADN LRL++VNHPD++FFVSHG Y +
Sbjct:  326 QNGTCEHQRVNSLVQAADNRLRLLEVNHPDFQFFVSHGMYRR 367

HSP 2 Score: 32.3426 bits (72), Expect = 6.275e-12
Identity = 13/16 (81.25%), Postives = 13/16 (81.25%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LP F MASYK KGSIW
Sbjct:  309 LPVFGMASYKFKGSIW 324
[Close]
BLAST of AM162151 vs. TrEMBL
Match: B9HX03_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_833344 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.056e-11
Identity = 29/38 (76.32%), Postives = 34/38 (89.47%), Query Frame = -2 
Query:  252 NGVSDNQLANSLLQAADNWLRLVQVNHPDYKFFVSHGT 365
            NGV + Q A+SLL+AADNWLRL+QVNHPDY+FFVSH T
Sbjct:  357 NGVYECQKASSLLRAADNWLRLLQVNHPDYRFFVSHNT 394

HSP 2 Score: 28.1054 bits (61), Expect = 1.056e-11
Identity = 11/16 (68.75%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LPTF +ASYK K S W
Sbjct:  339 LPTFGLASYKFKVSFW 354
[Close]
BLAST of AM162151 vs. TrEMBL
Match: B9N000_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_295925 PE=4 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.880e-10
Identity = 28/38 (73.68%), Postives = 33/38 (86.84%), Query Frame = -2 
Query:  252 NGVSDNQLANSLLQAADNWLRLVQVNHPDYKFFVSHGT 365
            NGV + Q + SLL+AADNWLRL+QVNHPDY+FFVSH T
Sbjct:  275 NGVYECQKSGSLLKAADNWLRLLQVNHPDYRFFVSHNT 312

HSP 2 Score: 25.409 bits (54), Expect = 1.880e-10
Identity = 9/16 (56.25%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LPTF +ASYK + + W
Sbjct:  257 LPTFGLASYKFEVTFW 272
[Close]
BLAST of AM162151 vs. TrEMBL
Match: B9HJ57_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_719815 PE=4 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.173e-9
Identity = 24/33 (72.73%), Postives = 28/33 (84.85%), Query Frame = -2 
Query:  267 NGVSDNQLANSLLQAADNWLRLVQVNHPDYKFF 365
            NG  D+QL+NSL QAAD WLRL+QVNHPD+ FF
Sbjct:  241 NGECDHQLSNSLFQAADKWLRLLQVNHPDFLFF 273

HSP 2 Score: 30.4166 bits (67), Expect = 1.173e-9
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LP F +ASYK KGS+W
Sbjct:  223 LPVFGLASYKFKGSLW 238
[Close]
BLAST of AM162151 vs. TrEMBL
Match: B9SM03_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1311110 PE=4 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.527e-9
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  267 NGVSDNQLANSLLQAADNWLRLVQVNHPDYKFF 365
            +G S+ QLANSLLQAADNWLRLVQV HPD+ FF
Sbjct:  384 SGGSERQLANSLLQAADNWLRLVQVYHPDFVFF 416

HSP 2 Score: 28.4906 bits (62), Expect = 2.527e-9
Identity = 10/16 (62.50%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LP F +ASYK KG +W
Sbjct:  366 LPVFGLASYKFKGPLW 381
[Close]
BLAST of AM162151 vs. TrEMBL
Match: Q9M9Q2_ARATH (Putative uncharacterized protein At1g15030 OS=Arabidopsis thaliana GN=At1g15030 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.539e-9
Identity = 25/32 (78.12%), Postives = 27/32 (84.38%), Query Frame = -2 
Query:  267 GVSDNQLANSLLQAADNWLRLVQVNHPDYKFF 362
            G S +QLANSL QAADNWLRL QVNHPD+ FF
Sbjct:  326 GGSGHQLANSLFQAADNWLRLRQVNHPDFIFF 357

HSP 2 Score: 31.187 bits (69), Expect = 2.539e-9
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LP F +ASYKL+GS+W
Sbjct:  307 LPVFGLASYKLRGSVW 322
[Close]
BLAST of AM162151 vs. TrEMBL
Match: D7SY72_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_77.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035053001 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.606e-9
Identity = 28/41 (68.29%), Postives = 35/41 (85.37%), Query Frame = -2 
Query:  243 NGVSDNQLANSLLQAADNWLRLVQVNHPDYKFFVSHGTYHK 365
            NGV++   ANSLL+AADNWLR +QVNHPDY+FFVSH +Y +
Sbjct:  387 NGVNECPKANSLLRAADNWLRSLQVNHPDYQFFVSHNSYRR 427
[Close]
BLAST of AM162151 vs. TrEMBL
Match: D7KCM3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_888896 PE=4 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.211e-8
Identity = 23/30 (76.67%), Postives = 26/30 (86.67%), Query Frame = -2 
Query:  267 SDNQLANSLLQAADNWLRLVQVNHPDYKFF 356
            S +Q+ANSL QAADNWLRL QVNHPD+ FF
Sbjct:  328 SGHQVANSLFQAADNWLRLRQVNHPDFIFF 357

HSP 2 Score: 31.187 bits (69), Expect = 1.211e-8
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LP F +ASYKL+GS+W
Sbjct:  307 LPVFGLASYKLRGSVW 322
[Close]
BLAST of AM162151 vs. TrEMBL
Match: B9RZK3_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0999300 PE=4 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.631e-8
Identity = 25/38 (65.79%), Postives = 29/38 (76.32%), Query Frame = -2 
Query:  252 NGVSDNQLANSLLQAADNWLRLVQVNHPDYKFFVSHGT 365
            NG  + Q  NSLL+AADNWLRL+QV HPDY FF SH +
Sbjct:  376 NGAYECQKVNSLLRAADNWLRLLQVYHPDYMFFASHNS 413

HSP 2 Score: 25.0238 bits (53), Expect = 2.631e-8
Identity = 10/16 (62.50%), Postives = 11/16 (68.75%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            L TF +ASYK K S W
Sbjct:  358 LATFGLASYKFKVSFW 373
[Close]
BLAST of AM162151 vs. TrEMBL
Match: B9HWD9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_658850 PE=4 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 2.635e-8
Identity = 24/33 (72.73%), Postives = 27/33 (81.82%), Query Frame = -2 
Query:  267 NGVSDNQLANSLLQAADNWLRLVQVNHPDYKFF 365
            NG  D QLANSLLQAA  +LRL+QVNHPD+ FF
Sbjct:  357 NGGGDRQLANSLLQAASKFLRLLQVNHPDFLFF 389

HSP 2 Score: 29.6462 bits (65), Expect = 2.635e-8
Identity = 11/16 (68.75%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LP F  ASYK KGS+W
Sbjct:  339 LPVFGFASYKFKGSLW 354
[Close]
BLAST of AM162151 vs. TAIR peptide
Match: AT1G15030.1 (| Symbols: | Protein of unknown function (DUF789) | chr1:5177895-5179853 FORWARD LENGTH=360)

HSP 1 Score: 53.9138 bits (128), Expect = 1.519e-11
Identity = 25/32 (78.12%), Postives = 27/32 (84.38%), Query Frame = -2 
Query:  267 GVSDNQLANSLLQAADNWLRLVQVNHPDYKFF 362
            G S +QLANSL QAADNWLRL QVNHPD+ FF
Sbjct:  326 GGSGHQLANSLFQAADNWLRLRQVNHPDFIFF 357

HSP 2 Score: 31.187 bits (69), Expect = 1.519e-11
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LP F +ASYKL+GS+W
Sbjct:  307 LPVFGLASYKLRGSVW 322
[Close]
BLAST of AM162151 vs. TAIR peptide
Match: AT5G49220.1 (| Symbols: | Protein of unknown function (DUF789) | chr5:19956627-19958453 FORWARD LENGTH=409)

HSP 1 Score: 49.6766 bits (117), Expect = 3.434e-10
Identity = 20/37 (54.05%), Postives = 29/37 (78.38%), Query Frame = -2 
Query:  258 KNGVSDNQLANSLLQAADNWLRLVQVNHPDYKFFVSH 368
            +N + ++Q   SLLQAAD WL+ +QV+HPDY+FF S+
Sbjct:  368 QNRIQESQKMTSLLQAADKWLKRLQVDHPDYRFFTSN 404

HSP 2 Score: 30.8018 bits (68), Expect = 3.434e-10
Identity = 12/16 (75.00%), Postives = 14/16 (87.50%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LPTF +ASYKLK S+W
Sbjct:  351 LPTFGLASYKLKVSVW 366
[Close]
BLAST of AM162151 vs. TAIR peptide
Match: AT2G01260.1 (| Symbols: | Protein of unknown function (DUF789) | chr2:135494-137504 REVERSE LENGTH=369)

HSP 1 Score: 44.2838 bits (103), Expect = 3.676e-8
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = -2 
Query:  267 GVSDNQLANSLLQAADNWLRLVQVNHPDYKFF 362
            G S++QL NSL QAAD WL    V+HPD+ FF
Sbjct:  335 GGSEHQLVNSLFQAADKWLHSCHVSHPDFLFF 366

HSP 2 Score: 29.261 bits (64), Expect = 3.676e-8
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = -1 
Query:  373 LPTFAMASYKLKGSIW 420
            LP F +ASYK +GS+W
Sbjct:  316 LPVFGLASYKFRGSLW 331
The following BLAST results are available for this feature:
BLAST of AM162151 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D7SJ29_VITVI6.275e-1261.90Whole genome shotgun sequence of line PN40024, sca... [more]
B9HX03_POPTR1.056e-1176.32Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9N000_POPTR1.880e-1073.68Predicted protein (Fragment) OS=Populus trichocarp... [more]
B9HJ57_POPTR1.173e-972.73Predicted protein (Fragment) OS=Populus trichocarp... [more]
B9SM03_RICCO2.527e-978.79Putative uncharacterized protein OS=Ricinus commun... [more]
Q9M9Q2_ARATH2.539e-978.13Putative uncharacterized protein At1g15030 OS=Arab... [more]
D7SY72_VITVI2.606e-968.29Whole genome shotgun sequence of line PN40024, sca... [more]
D7KCM3_ARALY1.211e-876.67Putative uncharacterized protein OS=Arabidopsis ly... [more]
B9RZK3_RICCO2.631e-865.79Putative uncharacterized protein OS=Ricinus commun... [more]
B9HWD9_POPTR2.635e-872.73Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
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BLAST of AM162151 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT1G15030.11.519e-1178.13| Symbols: | Protein of unknown function (DUF789)... [more]
AT5G49220.13.434e-1054.05| Symbols: | Protein of unknown function (DUF789)... [more]
AT2G01260.13.676e-859.38| Symbols: | Protein of unknown function (DUF789)... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM162151 ID=AM162151; Name=AM162151; organism=Pisum sativum; type=EST; length=420bp
ACATATTTCACTCATTCTTTCTAAATACAAAACTCTTTTTTTTTCCCCAC
TTATAATTGTTATTTTCACTTTAGCAACAAACTATATTTCCTTCTTCAAT
TGTGATCACTTCATATTTTGTGCTACTTAGAGGTGTAGAGTAACACAAAC
CCACATTAGCAGTCGCAACACCATAAAAATCTCTGTAGATCGGTCGATTA
TGCCATGAGAATGTAATGATATAAGTCGAGTCATTTGGTTCACTTGTGGT
ATGTGCCATGCGACACAAAGAACTTATAATCCGGGTGATTCACTTGAACC
AGCCGTAACCAGTTATCTGCAGCCTGCAAGAGCGAATTCGCTAACTGATT
GTCACTAACTCCGTTTTTACATCCAAATAGATCCCTTTAACTTATAAGAT
GCCATTGCAAAAGTTGGTAA
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