CD860180

EST Overview


Name	CD860180
Unique Name	CD860180
Type	EST
Organism	Pisum sativum (pea)
Sequence length	615

Libraries


Library Name	Type
LIBEST_014002 PW	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v2	Pisum sativum unigene v2	EST
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Pisum sativum unigene v2	2011-03-01
Homology Analysis: Pisum sativum unigene v2 vs Trembl	2011-04-27
InterProScan analysis for Pisum sativum unigene v2	2011-04-27
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD860180 vs. TrEMBL
Match: Q4W8M2_9BACI (SC protease OS=Bacillus sp. KSM-LD1 PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.563e-7
Identity = 42/149 (28.19%), Postives = 65/149 (43.62%), Query Frame = -1

Query:   25 LKDHADVVQADVTAMLEKARVAGKRHYLDQLWITNELIVRDIDRETVEQLRNHPDVESLVAEQFIQLEETEEGESFDLRDYNNTIQN---QWGVVNVNAPAAWARXXXXXXXXXXXXXXGARHTHVSLAPTYRGNNPGE----THDYNW 450
            L+  A   QADV A LEK   AG    +   +I N + V    +E +E++ + P+VE +   + +QL  + E  +    +  NT  N   +W +  V AP AW                G +  H +L   YRG +P      TH Y++
Sbjct:  109 LRSTAISTQADVKAYLEKQEAAGNAKDIQSFFIVNGMAV-TATKEVMEKIASFPEVEMITPNEEVQLYPSVEAPAGTTENDKNTPTNDNVEWNIQQVGAPQAWEMGVDGQGAVVAVMDTGVQWNHPALMQQYRGFDPANPNSPTHTYSF 256

[Close]

BLAST of CD860180 vs. TrEMBL
Match: Q4W8M2_9BACI (SC protease OS=Bacillus sp. KSM-LD1 PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 3.145e-7
Identity = 42/149 (28.19%), Postives = 65/149 (43.62%), Query Frame = -1

Query:   25 LKDHADVVQADVTAMLEKARVAGKRHYLDQLWITNELIVRDIDRETVEQLRNHPDVESLVAEQFIQLEETEEGESFDLRDYNNTIQN---QWGVVNVNAPAAWARXXXXXXXXXXXXXXGARHTHVSLAPTYRGNNPGE----THDYNW 450
            L+  A   QADV A LEK   AG    +   +I N + V    +E +E++ + P+VE +   + +QL  + E  +    +  NT  N   +W +  V AP AW                G +  H +L   YRG +P      TH Y++
Sbjct:  109 LRSTAISTQADVKAYLEKQEAAGNAKDIQSFFIVNGMAV-TATKEVMEKIASFPEVEMITPNEEVQLYPSVEAPAGTTENDKNTPTNDNVEWNIQQVGAPQAWEMGVDGQGAVVAVMDTGVQWNHPALMQQYRGFDPANPNSPTHTYSF 256

[Close]

BLAST of CD860180 vs. TrEMBL
Match: E5WDV6_9BACI (Bacillopeptidase F OS=Bacillus sp. 2_A_57_CT2 GN=HMPREF1013_00630 PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 5.365e-7
Identity = 38/146 (26.03%), Postives = 62/146 (42.47%), Query Frame = -1

Query:   25 LKDHADVVQADVTAMLEKARVAGKRHYLDQLWITNELIVRDIDRETVEQLRNHPDVESLVAEQFIQLEETEEGESFDLRDYNNTIQNQWGVVNVNAPAAWARXXXXXXXXXXXXXXGARHTHVSLAPTYRGNNPGE----THDYNW 450
            L+  A   Q  VT  L+K    GK   +   +I N + V    ++ +EQ+   P+VE ++  +  QL +  +     L+    T   +W +  V APA W                G +  H +L   YRG NP +    TH+++W
Sbjct:  113 LRSTAIETQGQVTNFLQKQEKDGKAKDVQSFYIVNAIAVT-ATKDVMEQIAMFPEVEKILPNETRQLHKPVKEAEKALKTQAETSSIEWNIDRVGAPAVWEMGIDGSGTVVASIDTGVQWNHPALKEKYRGYNPAQPDSPTHEFSW 257

The following BLAST results are available for this feature:

BLAST of CD860180 vs. TrEMBL
BLAST of CD860180 vs. TrEMBL

BLAST of CD860180 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 1


Match Name	E-value	Identity	Description
Q4W8M2_9BACI	2.563e-7	28.19	SC protease OS=Bacillus sp. KSM-LD1 PE=3 SV=1	[more]

BLAST of CD860180 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 2


Match Name	E-value	Identity	Description
Q4W8M2_9BACI	3.145e-7	28.19	SC protease OS=Bacillus sp. KSM-LD1 PE=3 SV=1	[more]
E5WDV6_9BACI	5.365e-7	26.03	Bacillopeptidase F OS=Bacillus sp. 2_A_57_CT2 GN=H...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 29..53 score
None	No IPR available	SEG	seg	seg	coord: 1..13 score
None	No IPR available	PANTHER	PTHR10795:SF32	SUBTILISIN	coord: 45..137 score: 9.7
None	No IPR available	SEG	seg	seg	coord: 106..120 score
None	No IPR available	SEG	seg	seg	coord: 9..32 score
IPR000209	Peptidase S8/S53, subtilisin/kexin/sedolisin	GENE3D	3.40.50.200	no description	coord: 86..135 score: 1
IPR000209	Peptidase S8/S53, subtilisin/kexin/sedolisin	PFAM	PF00082	Peptidase_S8	coord: 96..136 score: 1.2
IPR000209	Peptidase S8/S53, subtilisin/kexin/sedolisin	SUPERFAMILY	52743	Subtilisin-like	coord: 53..132 score: 8.4
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	SUBTILISIN/KEXIN-RELATED SERINE PROTEASE	coord: 45..137 score: 9.7

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 29..53 score
None	No IPR available	SEG	seg	seg	coord: 1..13 score
None	No IPR available	PANTHER	PTHR10795:SF32	SUBTILISIN	coord: 45..137 score: 9.7
None	No IPR available	SEG	seg	seg	coord: 106..120 score
None	No IPR available	SEG	seg	seg	coord: 9..32 score
IPR000209	Peptidase S8/S53, subtilisin/kexin/sedolisin	GENE3D	3.40.50.200	no description	coord: 86..135 score: 1
IPR000209	Peptidase S8/S53, subtilisin/kexin/sedolisin	PFAM	PF00082	Peptidase_S8	coord: 96..136 score: 1.2
IPR000209	Peptidase S8/S53, subtilisin/kexin/sedolisin	SUPERFAMILY	52743	Subtilisin-like	coord: 53..132 score: 8.4
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	SUBTILISIN/KEXIN-RELATED SERINE PROTEASE	coord: 45..137 score: 9.7

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v2
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD860180 ID=CD860180; Name=CD860180; organism=Pisum sativum; type=EST; length=615bp
GGCAGCGTTACCAGTTGGTGCACGCCAGTTATAATCGTGAGTCTCTCCGG
GGTTGTTACCGCGATATGTTGGTGCCAAAGATACGTGAGTGTGACGTGCT
CCAGTGTCAATGTTTCCAATGGTGACTCCTGTTCCGGTGATTCCTCTAGC
CCATGCAGCTGGGGCATTGACATTCACGACTCCCCATTGATTTTGGATGG
TATTGTTATAATCTCTGAGGTCGAAGCTTTCGCCTTCTTCGGTCTCTTCC
AATTGGATGAATTGTTCAGCAACCAAGGATTCAACGTCTGGGTGGTTTCT
CAATTGTTCAACGGTTTCTCTGTCGATATCGCGAACGATCAATTCGTTTG
TGATCCATAGTTGATCGAGGTAGTGTCTCTTGCCAGCAACTCTAGCCTTC
TCCAACATTGCGGTAACATCAGCTTGAACAACATCAGCATGATCCTTAAG
GATGGCGTGTTAAGTTTCCAAAATGGTAACACGGCTTGTGAGTCTCAACG
AGTAGAAACGATCCCATGCAGACTTNGGTGGTCGGCTCTTCGCGAAAGTG
ATCATNAACGTTGGTCAGTTCCTGATTCTTCAAGAATTTCTTGTAAATTT
TGTTCCTTCTACTCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004252	serine-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR000209	Peptidase_S8/S53_dom
IPR015500	Peptidase_S8_subtilisin-rel

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