GH720329

EST Overview


Name	GH720329
Unique Name	GH720329
Type	EST
Organism	Pisum sativum (pea)
Sequence length	300

Libraries


Library Name	Type
LIBEST_024270 552I	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720329 vs. TrEMBL
Match: C6ZRR5_SOYBN (Protein kinase family protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 2.167e-29
Identity = 44/49 (89.80%), Postives = 47/49 (95.92%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSNVLLSEDFEP+LSDFGLA WAST+SS+I CTDVAGTFGYMAPEY
Sbjct:  428 DVKSSNVLLSEDFEPQLSDFGLAKWASTTSSHIICTDVAGTFGYMAPEY 476

HSP 2 Score: 58.9214 bits (141), Expect = 2.167e-29
Identity = 27/40 (67.50%), Postives = 29/40 (72.50%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KKNP  FGW +RYKVA GVAEAL+YLH  D   VIHR  K
Sbjct:  391 KKNPLMFGWTERYKVAIGVAEALEYLHNNDGQSVIHRDVK 430

[Close]

BLAST of GH720329 vs. TrEMBL
Match: B9H7J5_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_714592 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.031e-28
Identity = 40/49 (81.63%), Postives = 45/49 (91.84%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA WA TSSS+I C DVAGTFGY+APEY
Sbjct:  476 DVKSSNILLSDDFEPQLSDFGLAKWAPTSSSHIICNDVAGTFGYLAPEY 524

HSP 2 Score: 60.4622 bits (145), Expect = 3.031e-28
Identity = 27/40 (67.50%), Postives = 30/40 (75.00%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK+PR FGW +RYKVA G+AEALDYLH     PVIHR  K
Sbjct:  439 KKDPRAFGWNERYKVALGIAEALDYLHSCSAQPVIHRDVK 478

[Close]

BLAST of GH720329 vs. TrEMBL
Match: B9GTZ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_816250 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 3.943e-28
Identity = 41/49 (83.67%), Postives = 46/49 (93.88%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA WA TSSS+I CTDVAGTFGY+APEY
Sbjct:  520 DVKSSNILLSDDFEPQLSDFGLAKWAPTSSSHIICTDVAGTFGYLAPEY 568

HSP 2 Score: 58.151 bits (139), Expect = 3.943e-28
Identity = 27/40 (67.50%), Postives = 29/40 (72.50%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK+P  FGW +RYKVA GVAEALDYLH     PVIHR  K
Sbjct:  483 KKDPLTFGWNERYKVALGVAEALDYLHSCSAQPVIHRDVK 522

[Close]

BLAST of GH720329 vs. TrEMBL
Match: B9RRF8_RICCO (ATP binding protein, putative OS=Ricinus communis GN=RCOM_1189890 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.922e-27
Identity = 40/49 (81.63%), Postives = 47/49 (95.92%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA+W STSSS++ CTDVAGTFGY+APEY
Sbjct:  451 DVKSSNILLSDDFEPQLSDFGLASWVSTSSSHMACTDVAGTFGYLAPEY 499

HSP 2 Score: 55.4546 bits (132), Expect = 1.922e-27
Identity = 26/40 (65.00%), Postives = 28/40 (70.00%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK+   FGW  R+KVA GVAEALDYLH   D PVIHR  K
Sbjct:  414 KKDGNSFGWQGRFKVAVGVAEALDYLHSFCDQPVIHRDVK 453

[Close]

BLAST of GH720329 vs. TrEMBL
Match: B9S2T6_RICCO (ATP binding protein, putative OS=Ricinus communis GN=RCOM_0561590 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 3.253e-27
Identity = 42/49 (85.71%), Postives = 47/49 (95.92%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA WASTSSS+I CTDVAGTFGY+APEY
Sbjct:  537 DVKSSNILLSDDFEPQLSDFGLAKWASTSSSHIICTDVAGTFGYLAPEY 585

HSP 2 Score: 53.1434 bits (126), Expect = 3.253e-27
Identity = 24/40 (60.00%), Postives = 28/40 (70.00%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            +K+P  F W +RYKVA GVAEAL+YLH     PVIHR  K
Sbjct:  500 RKDPLAFNWYERYKVAVGVAEALNYLHTGTAQPVIHRDVK 539

[Close]

BLAST of GH720329 vs. TrEMBL
Match: D7KUH5_ARALY (Kinase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_476891 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.681e-26
Identity = 38/49 (77.55%), Postives = 46/49 (93.88%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA WAS S+++I C+DVAGTFGY+APEY
Sbjct:  569 DVKSSNILLSDDFEPQLSDFGLARWASISTTHIICSDVAGTFGYLAPEY 617

HSP 2 Score: 56.225 bits (134), Expect = 2.681e-26
Identity = 26/40 (65.00%), Postives = 29/40 (72.50%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK+P  F W++RYKVA GVAEALDYLH     PVIHR  K
Sbjct:  532 KKDPLAFCWSERYKVAVGVAEALDYLHNTASQPVIHRDVK 571

[Close]

BLAST of GH720329 vs. TrEMBL
Match: O80661_ARATH (T14N5.13 protein OS=Arabidopsis thaliana GN=T14N5.13 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.681e-26
Identity = 38/49 (77.55%), Postives = 46/49 (93.88%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA WAS S+++I C+DVAGTFGY+APEY
Sbjct:  528 DVKSSNILLSDDFEPQLSDFGLARWASISTTHIICSDVAGTFGYLAPEY 576

HSP 2 Score: 56.225 bits (134), Expect = 2.681e-26
Identity = 26/40 (65.00%), Postives = 29/40 (72.50%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK+P  F W++RYKVA GVAEALDYLH     PVIHR  K
Sbjct:  491 KKDPLAFCWSERYKVAVGVAEALDYLHNTASQPVIHRDVK 530

[Close]

BLAST of GH720329 vs. TrEMBL
Match: B9HEY9_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_217237 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 6.348e-25
Identity = 40/49 (81.63%), Postives = 46/49 (93.88%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA+WASTS +  TCTDVAGTFGY+APEY
Sbjct:  501 DVKSSNILLSDDFEPQLSDFGLASWASTSCN-ATCTDVAGTFGYLAPEY 548

HSP 2 Score: 52.7582 bits (125), Expect = 6.348e-25
Identity = 25/40 (62.50%), Postives = 28/40 (70.00%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK+    GW +RYKVA GVAEALDYLH   D PVIH+  K
Sbjct:  464 KKDWNAVGWQERYKVAVGVAEALDYLHNCCDQPVIHKDVK 503

[Close]

BLAST of GH720329 vs. TrEMBL
Match: B9H4K7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_558448 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 8.262e-25
Identity = 40/49 (81.63%), Postives = 45/49 (91.84%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA WAST S + TCTDVAGTFGY+APEY
Sbjct:  502 DVKSSNILLSDDFEPQLSDFGLACWAST-SCHTTCTDVAGTFGYLAPEY 549

HSP 2 Score: 52.7582 bits (125), Expect = 8.262e-25
Identity = 24/40 (60.00%), Postives = 28/40 (70.00%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            +K+    GW +RYKVA GVAEALDYLH   D PVIH+  K
Sbjct:  465 RKDVNAIGWQERYKVAVGVAEALDYLHNCCDQPVIHKDVK 504

[Close]

BLAST of GH720329 vs. TrEMBL
Match: A5C274_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_043580 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.399e-24
Identity = 37/49 (75.51%), Postives = 44/49 (89.80%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA WA+T +S+  C DVAGTFGY+APEY
Sbjct:  520 DVKSSNILLSDDFEPQLSDFGLAAWANT-ASHKNCPDVAGTFGYLAPEY 567

HSP 2 Score: 56.9954 bits (136), Expect = 1.399e-24
Identity = 26/40 (65.00%), Postives = 29/40 (72.50%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK+P  FGW +RY+VA GVAEALDYLH     PVIHR  K
Sbjct:  483 KKDPMTFGWGERYRVALGVAEALDYLHNGCGQPVIHRDVK 522

[Close]

BLAST of GH720329 vs. SwissProt
Match: CRK3_ARATH (Cysteine-rich receptor-like protein kinase 3 OS=Arabidopsis thaliana GN=CRK3 PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 6.428e-14
Identity = 27/50 (54.00%), Postives = 37/50 (74.00%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEYL 285
            D+K SN+LL +DF PR++DFGLA       ++I+ T +AGT GYMAPEY+
Sbjct:  448 DIKLSNILLEDDFTPRIADFGLARLFPEDKTHIS-TAIAGTLGYMAPEYV 496

HSP 2 Score: 38.1206 bits (87), Expect = 6.428e-14
Identity = 15/41 (36.59%), Postives = 26/41 (63.41%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCKV 146
            +K+ +   WA+R+K+  G AE + YLH + +  +IHR  K+
Sbjct:  411 RKDVQPLNWAKRFKIILGTAEGMAYLHEESNLRIIHRDIKL 451

[Close]

BLAST of GH720329 vs. SwissProt
Match: PBS1_ARATH (Serine/threonine-protein kinase PBS1 OS=Arabidopsis thaliana GN=PBS1 PE=1 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 1.844e-13
Identity = 28/49 (57.14%), Postives = 31/49 (63.27%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D KSSN+LL E F P+LSDFGLA    T       T V GT+GY APEY
Sbjct:  213 DFKSSNILLDEGFHPKLSDFGLAKLGPTGDKSHVSTRVMGTYGYCAPEY 261

HSP 2 Score: 36.965 bits (84), Expect = 1.844e-13
Identity = 16/32 (50.00%), Postives = 22/32 (68.75%), Query Frame = 3

Query:   48 WAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            W  R K+AAG A+ L++LH K + PVI+R  K
Sbjct:  184 WNMRMKIAAGAAKGLEFLHDKANPPVIYRDFK 215

[Close]

BLAST of GH720329 vs. SwissProt
Match: IKU2_ARATH (Receptor-like protein kinase HAIKU2 OS=Arabidopsis thaliana GN=IKU2 PE=1 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 3.054e-13
Identity = 26/51 (50.98%), Postives = 34/51 (66.67%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLA--TWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LL E++ PR++DFGLA    A +     +   V GT GY+APEY
Sbjct:  814 DVKSSNILLDEEWRPRIADFGLAKIIQADSVQRDFSAPLVKGTLGYIAPEY 864

HSP 2 Score: 43.5134 bits (101), Expect = 3.054e-13
Identity = 19/46 (41.30%), Postives = 26/46 (56.52%), Query Frame = 3

Query:    6 WSRPRYKKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            W +   ++  +E GW  R  +A G A+ L+YLH   D PVIHR  K
Sbjct:  771 WEQLHERRGEQEIGWRVRQALALGAAKGLEYLHHGLDRPVIHRDVK 816

[Close]

BLAST of GH720329 vs. SwissProt
Match: RLCK7_ARATH (Probable serine/threonine-protein kinase RLCKVII OS=Arabidopsis thaliana GN=At1g07870 PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.494e-12
Identity = 25/49 (51.02%), Postives = 33/49 (67.35%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D+K SN+LL ED++P+LSDFGLA    +       T V GT+GY AP+Y
Sbjct:  230 DLKCSNILLGEDYQPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDY 278

HSP 2 Score: 35.8094 bits (81), Expect = 1.494e-12
Identity = 16/32 (50.00%), Postives = 20/32 (62.50%), Query Frame = 3

Query:   48 WAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            W  R K+AAG A  L+YLH +   PVI+R  K
Sbjct:  201 WNTRMKIAAGAARGLEYLHDRMTPPVIYRDLK 232

[Close]

BLAST of GH720329 vs. SwissProt
Match: CRK27_ARATH (Cysteine-rich receptor-like protein kinase 27 OS=Arabidopsis thaliana GN=CRK27 PE=2 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 4.201e-12
Identity = 25/49 (51.02%), Postives = 32/49 (65.31%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D+KSSNVLL E   P++SDFG+A      ++      V GT+GYMAPEY
Sbjct:  458 DLKSSNVLLDEQMLPKISDFGMARQFDFDNTQAVTRRVVGTYGYMAPEY 506

HSP 2 Score: 36.5798 bits (83), Expect = 4.201e-12
Identity = 14/36 (38.89%), Postives = 21/36 (58.33%), Query Frame = 3

Query:   36 REFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            ++  W +RY +  GV+  L YLH   + P+IHR  K
Sbjct:  425 KQLDWEKRYNIIVGVSRGLLYLHEGSEFPIIHRDLK 460

[Close]

BLAST of GH720329 vs. SwissProt
Match: Y2296_ARATH (Probable receptor-like protein kinase At2g42960 OS=Arabidopsis thaliana GN=At2g42960 PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 4.229e-12
Identity = 27/49 (55.10%), Postives = 37/49 (75.51%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D+K+SN+L+ ++F  +LSDFGLA    +  S+IT T V GTFGY+APEY
Sbjct:  309 DIKASNILIDDEFNAKLSDFGLAKLLDSGESHIT-TRVMGTFGYVAPEY 356

HSP 2 Score: 31.9574 bits (71), Expect = 4.229e-12
Identity = 14/32 (43.75%), Postives = 18/32 (56.25%), Query Frame = 3

Query:   48 WAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            W  R K+  G A+AL YLH   +  V+HR  K
Sbjct:  280 WEARMKIITGTAQALAYLHEAIEPKVVHRDIK 311

[Close]

BLAST of GH720329 vs. SwissProt
Match: BRL1_ARATH (Serine/threonine-protein kinase BRI1-like 1 OS=Arabidopsis thaliana GN=BRL1 PE=1 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 5.373e-12
Identity = 27/49 (55.10%), Postives = 37/49 (75.51%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D+KSSNVLL EDFE R+SDFG+A   S   ++++ + +AGT GY+ PEY
Sbjct:  987 DMKSSNVLLDEDFEARVSDFGMARLVSALDTHLSVSTLAGTPGYVPPEY 1035

HSP 2 Score: 30.8018 bits (68), Expect = 5.373e-12
Identity = 16/40 (40.00%), Postives = 19/40 (47.50%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK      WA R K+A G A  L +LH      +IHR  K
Sbjct:  950 KKGGIYLNWAARKKIAIGAARGLAFLHHSCIPHIIHRDMK 989

[Close]

BLAST of GH720329 vs. SwissProt
Match: BRL2_ARATH (Serine/threonine-protein kinase BRI1-like 2 OS=Arabidopsis thaliana GN=BRL1 PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 5.375e-12
Identity = 25/49 (51.02%), Postives = 36/49 (73.47%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D+KSSNVLL +D E R+SDFG+A   S   ++++ + +AGT GY+ PEY
Sbjct:  966 DMKSSNVLLDQDMEARVSDFGMARLISALDTHLSVSTLAGTPGYVPPEY 1014

HSP 2 Score: 34.6538 bits (78), Expect = 5.375e-12
Identity = 17/43 (39.53%), Postives = 23/43 (53.49%), Query Frame = 3

Query:   15 PRYKKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            PR  +  R  GW +R K+A G A+ L +LH      +IHR  K
Sbjct:  926 PRTGEKRRILGWEERKKIAKGAAKGLCFLHHNCIPHIIHRDMK 968

[Close]

BLAST of GH720329 vs. SwissProt
Match: RKF1_ARATH (Probable LRR receptor-like serine/threonine-protein kinase RFK1 OS=Arabidopsis thaliana GN=RKF1 PE=1 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 5.390e-12
Identity = 26/49 (53.06%), Postives = 36/49 (73.47%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D+K++N+LL +D  P++SDFGLA       ++I+ T VAGT GYMAPEY
Sbjct:  807 DIKATNILLDKDLTPKISDFGLARLDEEEKTHIS-TKVAGTIGYMAPEY 854

HSP 2 Score: 33.4982 bits (75), Expect = 5.390e-12
Identity = 14/43 (32.56%), Postives = 25/43 (58.14%), Query Frame = 3

Query:   15 PRYKKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            P++K+ P +  W  R+K+  G+A+ L +LH +     +HR  K
Sbjct:  769 PKHKQIPMD--WPTRFKICCGIAKGLAFLHEESPLKFVHRDIK 809

[Close]

BLAST of GH720329 vs. SwissProt
Match: RPK1_IPONI (Receptor-like protein kinase OS=Ipomoea nil GN=INRPK1 PE=2 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 6.982e-12
Identity = 24/48 (50.00%), Postives = 30/48 (62.50%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPE 279
            D+K  N+LL  D EP +SDFG+A     S++ I    V GT GYMAPE
Sbjct:  942 DIKPMNILLDSDLEPHISDFGIAKLLDQSATSIPSNTVQGTIGYMAPE 989

HSP 2 Score: 36.5798 bits (83), Expect = 6.982e-12
Identity = 14/42 (33.33%), Postives = 22/42 (52.38%), Query Frame = 3

Query:   33 PREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCKVLQCI 158
            P+   W+ R+ +A G A  L YLH   D  ++HR  K +  +
Sbjct:  908 PKPLDWSTRHNIAVGTAHGLAYLHFDCDPAIVHRDIKPMNIL 949

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT1G77280.1 (| Symbols: | Protein kinase protein with adenine nucleotide alpha hydrolases-like domain | chr1:29031468-29035882 REVERSE LENGTH=794)

HSP 1 Score: 86.2705 bits (212), Expect = 1.022e-28
Identity = 38/49 (77.55%), Postives = 46/49 (93.88%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA WAS S+++I C+DVAGTFGY+APEY
Sbjct:  570 DVKSSNILLSDDFEPQLSDFGLARWASISTTHIICSDVAGTFGYLAPEY 618

HSP 2 Score: 56.225 bits (134), Expect = 1.022e-28
Identity = 26/40 (65.00%), Postives = 29/40 (72.50%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK+P  F W++RYKVA GVAEALDYLH     PVIHR  K
Sbjct:  533 KKDPLAFCWSERYKVAVGVAEALDYLHNTASQPVIHRDVK 572

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT1G21590.1 (| Symbols: | Protein kinase protein with adenine nucleotide alpha hydrolases-like domain | chr1:7566613-7569694 REVERSE LENGTH=756)

HSP 1 Score: 85.1149 bits (209), Expect = 1.175e-26
Identity = 38/49 (77.55%), Postives = 45/49 (91.84%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSN+LLS+DFEP+LSDFGLA WAS S++ I C+DVAGTFGY+APEY
Sbjct:  534 DVKSSNILLSDDFEPQLSDFGLAKWASESTTQIICSDVAGTFGYLAPEY 582

HSP 2 Score: 50.447 bits (119), Expect = 1.175e-26
Identity = 24/40 (60.00%), Postives = 27/40 (67.50%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            KK+   F W +RYKVA G+AEALDYLH     PVIHR  K
Sbjct:  497 KKDLVAFRWNERYKVAVGIAEALDYLHNDAPQPVIHRDVK 536

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT5G63940.1 (| Symbols: | Protein kinase protein with adenine nucleotide alpha hydrolases-like domain | chr5:25588254-25591229 FORWARD LENGTH=705)

HSP 1 Score: 73.9442 bits (180), Expect = 6.112e-25
Identity = 33/49 (67.35%), Postives = 43/49 (87.76%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSNVLL++DFEP+LSDFG A+ AS++S ++   D+AGTFGY+APEY
Sbjct:  487 DVKSSNVLLADDFEPQLSDFGFASLASSTSQHVAGGDIAGTFGYLAPEY 535

HSP 2 Score: 55.8398 bits (133), Expect = 6.112e-25
Identity = 25/40 (62.50%), Postives = 30/40 (75.00%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            +K+ ++FGW +RYKVA GVAEALDYLH   D  VIHR  K
Sbjct:  450 RKDAKKFGWMERYKVAVGVAEALDYLHNTHDPEVIHRDVK 489

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT2G16750.1 (| Symbols: | Protein kinase protein with adenine nucleotide alpha hydrolases-like domain | chr2:7271786-7274446 FORWARD LENGTH=617)

HSP 1 Score: 73.9442 bits (180), Expect = 4.687e-21
Identity = 34/49 (69.39%), Postives = 39/49 (79.59%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            DVKSSNVLLS++FEP+LSDFGL+ W S S  Y    DV GTFGY+APEY
Sbjct:  399 DVKSSNVLLSDEFEPQLSDFGLSMWGSKSCRYTIQRDVVGTFGYLAPEY 447

HSP 2 Score: 42.743 bits (99), Expect = 4.687e-21
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = 3

Query:   48 WAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            W +R K+A G+ EALDYLH +  +PVIHR  K
Sbjct:  370 WEERLKIAIGLGEALDYLHNQCSNPVIHRDVK 401

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT4G35030.3 (| Symbols: | Protein kinase superfamily protein | chr4:16676234-16677962 FORWARD LENGTH=448)

HSP 1 Score: 65.0846 bits (157), Expect = 2.422e-19
Identity = 32/50 (64.00%), Postives = 39/50 (78.00%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWA-STSSSYITCTDVAGTFGYMAPEY 282
            DVK+SNVLLS + +P+LSDFGL+ W  +TSS Y    DV GTFGY+APEY
Sbjct:  230 DVKTSNVLLSLELQPQLSDFGLSMWGPTTSSRYSIQGDVVGTFGYLAPEY 279

HSP 2 Score: 45.8246 bits (107), Expect = 2.422e-19
Identity = 20/40 (50.00%), Postives = 26/40 (65.00%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            +K      W +R+K+A G+AEALDYLH +   PVIHR  K
Sbjct:  193 QKGKYVLSWEERFKIAIGLAEALDYLHNRCSKPVIHRDVK 232

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT4G35030.2 (| Symbols: | Protein kinase superfamily protein | chr4:16676234-16677684 FORWARD LENGTH=379)

HSP 1 Score: 65.0846 bits (157), Expect = 2.431e-19
Identity = 32/50 (64.00%), Postives = 39/50 (78.00%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWA-STSSSYITCTDVAGTFGYMAPEY 282
            DVK+SNVLLS + +P+LSDFGL+ W  +TSS Y    DV GTFGY+APEY
Sbjct:  230 DVKTSNVLLSLELQPQLSDFGLSMWGPTTSSRYSIQGDVVGTFGYLAPEY 279

HSP 2 Score: 45.8246 bits (107), Expect = 2.431e-19
Identity = 20/40 (50.00%), Postives = 26/40 (65.00%), Query Frame = 3

Query:   24 KKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            +K      W +R+K+A G+AEALDYLH +   PVIHR  K
Sbjct:  193 QKGKYVLSWEERFKIAIGLAEALDYLHNRCSKPVIHRDVK 232

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT1G66460.1 (| Symbols: | Protein kinase superfamily protein | chr1:24789894-24791988 REVERSE LENGTH=467)

HSP 1 Score: 62.3882 bits (150), Expect = 9.525e-18
Identity = 28/49 (57.14%), Postives = 34/49 (69.39%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D+K SN+LLS +  P+L DFGLATW +  S    C  V GTFGY+APEY
Sbjct:  262 DIKPSNILLSSNKIPKLCDFGLATWTAAPSVPFLCKTVKGTFGYLAPEY 310

HSP 2 Score: 43.1282 bits (100), Expect = 9.525e-18
Identity = 18/44 (40.91%), Postives = 27/44 (61.36%), Query Frame = 3

Query:   12 RPRYKKNPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            + + +K P    W+ RYKVA G+A+A+ YLH   +  V+HR  K
Sbjct:  221 KKKSRKTPLNLPWSTRYKVALGIADAIAYLHNGTEQCVVHRDIK 264

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT5G57670.2 (| Symbols: | Protein kinase superfamily protein | chr5:23360531-23363694 REVERSE LENGTH=579)

HSP 1 Score: 60.4622 bits (145), Expect = 1.693e-16
Identity = 27/50 (54.00%), Postives = 35/50 (70.00%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEYL 285
            D+KSSNVLL  D+EP+++DFGLA W     ++     V GTFGY+APE L
Sbjct:  392 DIKSSNVLLGPDYEPQITDFGLAKWLPNKWTHHAVIPVEGTFGYLAPESL 441

HSP 2 Score: 40.817 bits (94), Expect = 1.693e-16
Identity = 17/32 (53.12%), Postives = 21/32 (65.62%), Query Frame = 3

Query:   48 WAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            W  RYK+A GVA  L YLH + +H +IHR  K
Sbjct:  363 WPVRYKIAVGVARGLHYLHKRCNHRIIHRDIK 394

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT2G18890.1 (| Symbols: | Protein kinase superfamily protein | chr2:8184027-8186685 FORWARD LENGTH=392)

HSP 1 Score: 66.2402 bits (160), Expect = 1.708e-16
Identity = 28/49 (57.14%), Postives = 39/49 (79.59%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D+KSSNVLL++DFEP++SDFGLA W  +  S+ +   + GTFG++APEY
Sbjct:  195 DIKSSNVLLNQDFEPQISDFGLAKWLPSQWSHHSIAPIEGTFGHLAPEY 243

HSP 2 Score: 35.039 bits (79), Expect = 1.708e-16
Identity = 16/38 (42.11%), Postives = 19/38 (50.00%), Query Frame = 3

Query:   30 NPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            N     W  RYK+A G A+ L YLH      +IHR  K
Sbjct:  160 NQAPLEWETRYKIAIGTAKGLHYLHKGCQRRIIHRDIK 197

[Close]

BLAST of GH720329 vs. TAIR peptide
Match: AT2G18890.2 (| Symbols: | Protein kinase superfamily protein | chr2:8184027-8185673 FORWARD LENGTH=277)

HSP 1 Score: 66.2402 bits (160), Expect = 1.722e-16
Identity = 28/49 (57.14%), Postives = 39/49 (79.59%), Query Frame = 1

Query:  136 DVKSSNVLLSEDFEPRLSDFGLATWASTSSSYITCTDVAGTFGYMAPEY 282
            D+KSSNVLL++DFEP++SDFGLA W  +  S+ +   + GTFG++APEY
Sbjct:  195 DIKSSNVLLNQDFEPQISDFGLAKWLPSQWSHHSIAPIEGTFGHLAPEY 243

HSP 2 Score: 35.039 bits (79), Expect = 1.722e-16
Identity = 16/38 (42.11%), Postives = 19/38 (50.00%), Query Frame = 3

Query:   30 NPREFGWAQRYKVAAGVAEALDYLHCKDDHPVIHRGCK 143
            N     W  RYK+A G A+ L YLH      +IHR  K
Sbjct:  160 NQAPLEWETRYKIAIGTAKGLHYLHKGCQRRIIHRDIK 197

The following BLAST results are available for this feature:

BLAST of GH720329 vs. TrEMBL
BLAST of GH720329 vs. SwissProt
BLAST of GH720329 vs. TAIR peptide

BLAST of GH720329 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6ZRR5_SOYBN	2.167e-29	89.80	Protein kinase family protein OS=Glycine max PE=2 ...	[more]
B9H7J5_POPTR	3.031e-28	81.63	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9GTZ5_POPTR	3.943e-28	83.67	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RRF8_RICCO	1.922e-27	81.63	ATP binding protein, putative OS=Ricinus communis ...	[more]
B9S2T6_RICCO	3.253e-27	85.71	ATP binding protein, putative OS=Ricinus communis ...	[more]
D7KUH5_ARALY	2.681e-26	77.55	Kinase family protein OS=Arabidopsis lyrata subsp....	[more]
O80661_ARATH	2.681e-26	77.55	T14N5.13 protein OS=Arabidopsis thaliana GN=T14N5....	[more]
B9HEY9_POPTR	6.348e-25	81.63	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9H4K7_POPTR	8.262e-25	81.63	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5C274_VITVI	1.399e-24	75.51	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of GH720329 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CRK3_ARATH	6.428e-14	54.00	Cysteine-rich receptor-like protein kinase 3 OS=Ar...	[more]
PBS1_ARATH	1.844e-13	57.14	Serine/threonine-protein kinase PBS1 OS=Arabidopsi...	[more]
IKU2_ARATH	3.054e-13	50.98	Receptor-like protein kinase HAIKU2 OS=Arabidopsis...	[more]
RLCK7_ARATH	1.494e-12	51.02	Probable serine/threonine-protein kinase RLCKVII O...	[more]
CRK27_ARATH	4.201e-12	51.02	Cysteine-rich receptor-like protein kinase 27 OS=A...	[more]
Y2296_ARATH	4.229e-12	55.10	Probable receptor-like protein kinase At2g42960 OS...	[more]
BRL1_ARATH	5.373e-12	55.10	Serine/threonine-protein kinase BRI1-like 1 OS=Ara...	[more]
BRL2_ARATH	5.375e-12	51.02	Serine/threonine-protein kinase BRI1-like 2 OS=Ara...	[more]
RKF1_ARATH	5.390e-12	53.06	Probable LRR receptor-like serine/threonine-protei...	[more]
RPK1_IPONI	6.982e-12	50.00	Receptor-like protein kinase OS=Ipomoea nil GN=INR...	[more]

BLAST of GH720329 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G77280.1	1.022e-28	77.55	\| Symbols: \| Protein kinase protein with adenine ...	[more]
AT1G21590.1	1.175e-26	77.55	\| Symbols: \| Protein kinase protein with adenine ...	[more]
AT5G63940.1	6.112e-25	67.35	\| Symbols: \| Protein kinase protein with adenine ...	[more]
AT2G16750.1	4.687e-21	69.39	\| Symbols: \| Protein kinase protein with adenine ...	[more]
AT4G35030.3	2.422e-19	64.00	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT4G35030.2	2.431e-19	64.00	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT1G66460.1	9.525e-18	57.14	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT5G57670.2	1.693e-16	54.00	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT2G18890.1	1.708e-16	57.14	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT2G18890.2	1.722e-16	57.14	\| Symbols: \| Protein kinase superfamily protein \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase, catalytic domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 1..58 score: 13
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..58 score: 2.2
IPR017442	Serine/threonine-protein kinase-like domain	PFAM	PF00069	Pkinase	coord: 4..53 score: 3.3
None	No IPR available	GENE3D	1.10.510.10	no description	coord: 4..56 score: 1.3
None	No IPR available	PANTHER	PTHR23258	SERINE-THREONINE PROTEIN KINASE, PLANT-TYPE	coord: 4..53 score: 5.1
None	No IPR available	PANTHER	PTHR23258:SF425	SERINE/THREONINE PROTEIN KINASE-RELATED	coord: 4..53 score: 5.1
None	No IPR available	GENE3D	1.10.510.10	no description	coord: 9..45 score: 5.5
None	No IPR available	PANTHER	PTHR23258	SERINE-THREONINE PROTEIN KINASE, PLANT-TYPE	coord: 9..44 score: 1.6
None	No IPR available	PANTHER	PTHR23258:SF425	SERINE/THREONINE PROTEIN KINASE-RELATED	coord: 9..44 score: 1.6
None	No IPR available	SEG	seg	seg	coord: 24..35 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720329 ID=GH720329; Name=GH720329; organism=Pisum sativum; type=EST; length=300bp
TAGCGTGGTCGCGGCCGAGGTATAAGAAAAACCCTCGCGAGTTCGGTTGG
GCACAGAGATATAAGGTTGCTGCAGGTGTTGCCGAGGCTTTAGATTACCT
GCATTGTAAAGATGATCATCCTGTGATTCACCGGGGATGTAAAGTCCTCC
AATGTATTACTATCCGAAGATTTCGAACCGCGGCTATCTGATTTTGGATT
GGCTACGTGGGCATCAACCTCGTCATCATATATAACGTGCACAGATGTTG
CTGGAACCTTCGGTTATATGGCTCCCGAGTACCTGCCCGGGCGGCCGCTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR011009	Kinase-like_dom_sf
IPR017442	Serine/threonine-protein kinase-like domain

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