AM161865

EST Overview


Name	AM161865
Unique Name	AM161865
Type	EST
Organism	Pisum sativum (pea)
Sequence length	540

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v2	Pisum sativum unigene v2	EST
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Pisum sativum unigene v2	2011-03-01
Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Trembl	2011-04-27
Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Swissprot	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins	2011-04-21
InterProScan analysis for Pisum sativum unigene v2	2011-04-27
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM161865 vs. TrEMBL
Match: D7T9F5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00012030001 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 7.503e-29
Identity = 53/81 (65.43%), Postives = 59/81 (72.84%), Query Frame = 2

Query:  296 QNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            QNKQAGG IVMDADATSALHKR +APTDDSFKF WF+DH SV+KAIY+ GSEF  +   + G           FYAEQGGQ
Sbjct:  531 QNKQAGGTIVMDADATSALHKRGVAPTDDSFKFTWFQDHGSVIKAIYS-GSEF--LESASAGNEVGIVLETTSFYAEQGGQ 608

HSP 2 Score: 53.1434 bits (126), Expect = 7.503e-29
Identity = 24/30 (80.00%), Postives = 27/30 (90.00%), Query Frame = 3

Query:  162 AFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            AF LWDT+GFPLDLTQLMAEE+ L VDV+G
Sbjct:  486 AFVLWDTYGFPLDLTQLMAEERGLIVDVQG 515

[Close]

BLAST of AM161865 vs. TrEMBL
Match: B9H602_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_558710 PE=4 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.615e-24
Identity = 81/181 (44.75%), Postives = 97/181 (53.59%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTE-RGGSV*VVGYIRVPIRSDTAY-G*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            + DVFPELK+ E HIR   +     F + L  G  K         GK    +   V +VGYI +PIR D+   G R  +      FN             QNKQAGGAIVMDADATSALHK+ ++ TDDSFKF+ F+DHESV+KAIY  GSEF       +    V       FYAEQGGQ
Sbjct:  415 MSDVFPELKRNEEHIRETIAAEEASFGKTLLKGIEKFKKAAQEVEGKVLSGQARCVCLVGYIWLPIRFDSEERGLRVDVEG----FNNAMDEARERSRNAQNKQAGGAIVMDADATSALHKKGVSATDDSFKFICFEDHESVIKAIYN-GSEFLESAAAANEVGIV--LESTSFYAEQGGQ 588

[Close]

BLAST of AM161865 vs. TrEMBL
Match: D7T7J0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_39.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024320001 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.280e-21
Identity = 72/179 (40.22%), Postives = 87/179 (48.60%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVFPELKQ E+HIR + +     F + L  G  K         GK      +  +      P+           +T     FN             QNK+AGGAIVMDADAT+ALHK  +A TDD  KF WF+DHESV+KAIYT G EF       D    +       FYAEQGGQ
Sbjct:  367 MGDVFPELKQHEMHIRGIIAAEEASFGKTLIKGIEKFKKAAQEVQGKILSGQEAFVLWDTYGFPLDLTQLMAEERGLTVDVMGFNIAMDEARERSRNAQNKKAGGAIVMDADATAALHKMGVAATDDISKFTWFEDHESVIKAIYT-GIEFLESAAAGDEVGII--LETTSFYAEQGGQ 542

[Close]

BLAST of AM161865 vs. TrEMBL
Match: B9SYR5_RICCO (Alanyl-tRNA synthetase, putative OS=Ricinus communis GN=RCOM_0036070 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 6.352e-21
Identity = 70/179 (39.11%), Postives = 88/179 (49.16%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVFPEL Q EV IR +       F + L  G  K         GK +    +  +      P+           +      FN             QNKQAGG I+MDADATSALHK+ ++ TDDS+KF+WF+DHESV+KAIYT G+EF  +   T             FYAEQGGQ
Sbjct:  432 MGDVFPELIQNEVRIREIIKEEEASFGKTLLKGIEKFKKAAQEVQGKVSCLQDAFVLWDTYGFPLDLTQLMAEERGLWVDVEGFNNAMDEARERSRNAQNKQAGGDIIMDADATSALHKKGVSVTDDSYKFIWFQDHESVIKAIYT-GTEF--LGSTTSSNEVGIVLESTSFYAEQGGQ 607

[Close]

BLAST of AM161865 vs. TrEMBL
Match: D7KFW7_ARALY (Cytosolic tRNA-Ala synthetase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_474152 PE=4 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 9.172e-20
Identity = 69/179 (38.55%), Postives = 84/179 (46.93%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVF ELK+ E  I  +       F + L  G  K         G       +  +      P+          ++      FN             QNKQAGGAIVMDADATS LHK  ++ TDDSFK++WF+DHES +KAIYT GS F   L  TD    V       FYAEQGGQ
Sbjct:  362 MGDVFTELKEHEKKITEIIKEEEASFCKTLVKGIEKFQKAEQAVQGNTLSGEDAFVLWDTYGFPLDLTQLMAEERRLLVDVEGFNKAMEEARERSRSAQNKQAGGAIVMDADATSTLHKTGVSATDDSFKYIWFQDHESELKAIYT-GSTFLESLAATDNVGLV--LGSTSFYAEQGGQ 537

[Close]

BLAST of AM161865 vs. TrEMBL
Match: A9T4K7_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_140193 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 8.884e-15
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            + EEE SF RTLVKGI KFK A   V+   L  + AF LWDTFGFP+DLT+LMAEE  L+VD EG
Sbjct:  376 ISEEESSFSRTLVKGIEKFKKAAAEVKDGVLGAQDAFILWDTFGFPIDLTELMAEEMGLKVDKEG 440

HSP 2 Score: 57.7658 bits (138), Expect = 5.220e-7
Identity = 51/186 (27.42%), Postives = 73/186 (39.25%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYT-------PGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +G+ FPELK+ +  I+ + S     F + L  G  K     +           +  +      PI           +   +  FN             + K  G A+VM+A+ATS L K  I PTDD  K++W K+    VKAI+T          E   I+L T             +YAEQGGQ
Sbjct:  358 MGEAFPELKKNQAKIKEIISEEESSFSRTLVKGIEKFKKAAAEVKDGVLGAQDAFILWDTFGFPIDLTELMAEEMGLKVDKEGFNRTMEEAREKARGARGKAGGKALVMEAEATSQLQKLGILPTDDKPKYIWHKNPTVTVKAIFTSSGFEESTSDEDVGIVLDT-----------TSYYAEQGGQ 532

[Close]

BLAST of AM161865 vs. TrEMBL
Match: Q583T3_9TRYP (Alanyl-tRNA synthetase, putative OS=Trypanosoma brucei GN=Tb927.6.700 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.142e-14
Identity = 33/74 (44.59%), Postives = 43/74 (58.11%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQE 278
            L +EE SF RT   G+  F  AV     N +SGE AF L D +GFP+DLT L+AE+  + VD+EG     K  +
Sbjct:  378 LADEETSFARTWETGLKHFNKAVAEAVNNVISGENAFILHDRYGFPVDLTSLLAEKANMSVDLEGFHATMKASQ 451

HSP 2 Score: 36.965 bits (84), Expect = 3.142e-14
Identity = 26/81 (32.10%), Postives = 37/81 (45.68%), Query Frame = 2

Query:  308 AGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV----*FWNLQDFYAEQGGQ 538
            A     +D      L    ++PTDDS K+ W K H + VKAI+   S     +L+ D            ++ +FYAE GGQ
Sbjct:  458 AAAKTFIDVHQLEELKAGGVSPTDDSAKYTW-KKHVAEVKAIFDKKSGSFVDVLLPDSEMGPEDIGIILDVTNFYAEAGGQ 537

[Close]

BLAST of AM161865 vs. TrEMBL
Match: C9ZQ79_TRYBG (Alanyl-tRNA synthetase, putative OS=Trypanosoma brucei gambiense DAL972 GN=TbgDal_VI390 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.142e-14
Identity = 33/74 (44.59%), Postives = 43/74 (58.11%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQE 278
            L +EE SF RT   G+  F  AV     N +SGE AF L D +GFP+DLT L+AE+  + VD+EG     K  +
Sbjct:  378 LADEETSFARTWETGLKHFNKAVAEAVNNVISGENAFILHDRYGFPVDLTSLLAEKANMSVDLEGFHATMKASQ 451

HSP 2 Score: 36.965 bits (84), Expect = 3.142e-14
Identity = 26/81 (32.10%), Postives = 37/81 (45.68%), Query Frame = 2

Query:  308 AGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV----*FWNLQDFYAEQGGQ 538
            A     +D      L    ++PTDDS K+ W K H + VKAI+   S     +L+ D            ++ +FYAE GGQ
Sbjct:  458 AAAKTFIDVHQLEELKAGGVSPTDDSAKYTW-KKHVAEVKAIFDKKSGSFVDVLLPDSEMGPEDIGIILDVTNFYAEAGGQ 537

[Close]

BLAST of AM161865 vs. TrEMBL
Match: C1EDP7_9CHLO (Alanyl-tRNA synthetase OS=Micromonas sp. RCC299 GN=MICPUN_62346 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 4.084e-14
Identity = 37/81 (45.68%), Postives = 53/81 (65.43%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQG---NTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWK-LQEKGQ 287
            + EEEESFGRTL+KG+ +FK      +    +T+SG  AF LW++FGFP+DL ++M EE  ++VD  G    +K  QEK +
Sbjct:  376 IAEEEESFGRTLLKGVEQFKKIAAKAKEEGRDTVSGAEAFLLWESFGFPVDLVEIMCEENGMKVDNAGFEEAFKEAQEKSR 456

HSP 2 Score: 32.3426 bits (72), Expect = 4.084e-14
Identity = 26/79 (32.91%), Postives = 37/79 (46.84%), Query Frame = 2

Query:  302 KQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            K  G  ++ +A+AT+ L    +  TDDS K+      E+ VKAI T    F       +G   +   +   FYAE GGQ
Sbjct:  461 KGGGRQLLFEAEATAWLANNGVPLTDDSHKYAAGSVPEATVKAILTLDG-FVESTENVEGPIGL-VLDKTSFYAESGGQ 537

[Close]

BLAST of AM161865 vs. TrEMBL
Match: D3B7Q7_POLPA (Alanyl-tRNA synthetase OS=Polysphondylium pallidum PN500 GN=alaS PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 5.304e-14
Identity = 31/69 (44.93%), Postives = 45/69 (65.22%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVL 263
            ++ EE+ F RTL KGI +F+  ++      +S   A  L  +FGFP+DLT LMAEEK ++VD++G  VL
Sbjct:  378 IESEEKMFERTLDKGILEFENILKKTSNGVISASHAHYLSSSFGFPIDLTTLMAEEKGVKVDLKGYEVL 446

HSP 2 Score: 39.6614 bits (91), Expect = 5.304e-14
Identity = 30/82 (36.59%), Postives = 43/82 (52.44%), Query Frame = 2

Query:  296 QNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV-*FWNLQDFYAEQGGQ 538
            + K+    + + A+  S L KRSI PT+D +K+   K+ ++VVKAI+T G E    L        V       +FY EQGGQ
Sbjct:  457 RQKEKKSELNLGAEPISILQKRSIQPTNDQYKYEQ-KEVQTVVKAIWT-GKELIDQLTAEHKNTLVGVILESTNFYPEQGGQ 536

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_ARATH (Alanyl-tRNA synthetase, mitochondrial OS=Arabidopsis thaliana GN=ALATS PE=1 SV=3)

HSP 1 Score: 96.2857 bits (238), Expect = 1.001e-19
Identity = 67/179 (37.43%), Postives = 84/179 (46.93%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVF ELK+ E  I ++       F + L+ G  K         G       +  +      P+           +      FN             QNKQAGGAIVMDADATS LHK  ++ TDDSFK++WF+DHES +KAIYT GS F      +D    V       FYAEQGGQ
Sbjct:  410 MGDVFTELKEHEKKITDIIKEEEASFCKTLAKGIEKFRKAGQAVQGNTLSGDDAFILWDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNKQAGGAIVMDADATSTLHKAGVSATDDSFKYIWFQDHESELKAIYT-GSTFLESSAASDNVGLV--LGSTSFYAEQGGQ 585

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_DICDI (Alanyl-tRNA synthetase OS=Dictyostelium discoideum GN=alaS PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.251e-17
Identity = 34/79 (43.04%), Postives = 51/79 (64.56%), Query Frame = 3

Query:   45 LEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            ++M L  EE+ F +TL KGI +F+  ++    NTLS E A+ L   +GFP+DLT +MAEEK  +VD++G   L + Q +
Sbjct:  368 IKMVLTREEDMFNKTLEKGIVEFEKMIKKSVNNTLSAENAYFLSTCYGFPIDLTTIMAEEKNYKVDIKGYEGLCEAQSE 446

HSP 2 Score: 42.743 bits (99), Expect = 1.251e-17
Identity = 32/83 (38.55%), Postives = 43/83 (51.81%), Query Frame = 2

Query:  296 QNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHE--SVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            + K+    + + A+A + L    I PTDDSFK   +K HE  SV+KAI+  G EF  +     G          +FYAEQGGQ
Sbjct:  451 RQKEKKVELTLGAEANAWLKNNDIKPTDDSFK---YKQHEIQSVIKAIWN-GKEF--VDTAPKGTLIGVVLENSNFYAEQGGQ 527

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_ACTPJ (Alanyl-tRNA synthetase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.322e-13
Identity = 35/65 (53.85%), Postives = 48/65 (73.85%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            L+ EEE F RTL +G+A  + A+  V+ NTLSGEVAF+L+DT+GFPLDLT  +  E++L +D  G
Sbjct:  359 LKAEEEQFARTLERGLALLEDALTKVENNTLSGEVAFKLYDTYGFPLDLTADVCRERELTIDEAG 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_ACTP2 (Alanyl-tRNA synthetase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.322e-13
Identity = 35/65 (53.85%), Postives = 48/65 (73.85%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            L+ EEE F RTL +G+A  + A+  V+ NTLSGEVAF+L+DT+GFPLDLT  +  E++L +D  G
Sbjct:  359 LKAEEEQFARTLERGLALLEDALTKVENNTLSGEVAFKLYDTYGFPLDLTADVCRERELTIDEAG 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_LEGPH (Alanyl-tRNA synthetase OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.078e-13
Identity = 36/84 (42.86%), Postives = 55/84 (65.48%), Query Frame = 3

Query:   30 NRKCTLEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            N K  +E  LQ+EE  F RTL +G+   +  ++++QG  LSGEVAF+L+DT+GFP+DLT  +  E+ L +D+E    L + Q +
Sbjct:  340 NSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYGFPIDLTADIIREQGLHIDMEAFNQLMQQQRE 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_LEGPC (Alanyl-tRNA synthetase OS=Legionella pneumophila (strain Corby) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.078e-13
Identity = 36/84 (42.86%), Postives = 55/84 (65.48%), Query Frame = 3

Query:   30 NRKCTLEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            N K  +E  LQ+EE  F RTL +G+   +  ++++QG  LSGEVAF+L+DT+GFP+DLT  +  E+ L +D+E    L + Q +
Sbjct:  340 NSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYGFPIDLTADIIREQGLHIDMEAFNQLMQQQRE 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_ACTP7 (Alanyl-tRNA synthetase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.078e-13
Identity = 34/65 (52.31%), Postives = 48/65 (73.85%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            L+ EEE F RTL +G+A  + A+  V+ NTLSGEVAF+L+DT+GFPLDLT  +  E+++ +D  G
Sbjct:  359 LKAEEEQFARTLERGLALLEDALTKVENNTLSGEVAFKLYDTYGFPLDLTADVCREREITIDEAG 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_FRATW (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. tularensis (strain WY96-3418) GN=alaS PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.548e-12
Identity = 35/79 (44.30%), Postives = 55/79 (69.62%), Query Frame = 3

Query:   45 LEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            +E  L +EEE F +T+  GI  F   +++++ NT+SGEVAF+L+DT+GFP DLT  MA EK L+VD +  +   ++Q++
Sbjct:  346 IEKTLIKEEELFLKTIENGIKIFDAEIENLKDNTISGEVAFKLYDTYGFPFDLTADMAREKGLKVDEQAFLAQMQIQKQ 424

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_FRATT (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. tularensis GN=alaS PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.548e-12
Identity = 35/79 (44.30%), Postives = 55/79 (69.62%), Query Frame = 3

Query:   45 LEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            +E  L +EEE F +T+  GI  F   +++++ NT+SGEVAF+L+DT+GFP DLT  MA EK L+VD +  +   ++Q++
Sbjct:  346 IEKTLIKEEELFLKTIENGIKIFDAEIENLKDNTISGEVAFKLYDTYGFPFDLTADMAREKGLKVDEQAFLAQMQIQKQ 424

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_FRATO (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=alaS PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.548e-12
Identity = 35/79 (44.30%), Postives = 55/79 (69.62%), Query Frame = 3

Query:   45 LEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            +E  L +EEE F +T+  GI  F   +++++ NT+SGEVAF+L+DT+GFP DLT  MA EK L+VD +  +   ++Q++
Sbjct:  346 IEKTLIKEEELFLKTIENGIKIFDAEIENLKDNTISGEVAFKLYDTYGFPFDLTADMAREKGLKVDEQAFLAQMQIQKQ 424

[Close]

BLAST of AM161865 vs. TAIR peptide
Match: AT1G50200.2 (| Symbols: ALATS | Alanyl-tRNA synthetase | chr1:18591429-18598167 REVERSE LENGTH=963)

HSP 1 Score: 96.2857 bits (238), Expect = 1.091e-20
Identity = 67/179 (37.43%), Postives = 84/179 (46.93%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVF ELK+ E  I ++       F + L+ G  K         G       +  +      P+           +      FN             QNKQAGGAIVMDADATS LHK  ++ TDDSFK++WF+DHES +KAIYT GS F      +D    V       FYAEQGGQ
Sbjct:  362 MGDVFTELKEHEKKITDIIKEEEASFCKTLAKGIEKFRKAGQAVQGNTLSGDDAFILWDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNKQAGGAIVMDADATSTLHKAGVSATDDSFKYIWFQDHESELKAIYT-GSTFLESSAASDNVGLV--LGSTSFYAEQGGQ 537

[Close]

BLAST of AM161865 vs. TAIR peptide
Match: AT1G50200.1 (| Symbols: ALATS, ACD | Alanyl-tRNA synthetase | chr1:18591429-18598311 REVERSE LENGTH=1003)

HSP 1 Score: 96.2857 bits (238), Expect = 1.091e-20
Identity = 67/179 (37.43%), Postives = 84/179 (46.93%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVF ELK+ E  I ++       F + L+ G  K         G       +  +      P+           +      FN             QNKQAGGAIVMDADATS LHK  ++ TDDSFK++WF+DHES +KAIYT GS F      +D    V       FYAEQGGQ
Sbjct:  410 MGDVFTELKEHEKKITDIIKEEEASFCKTLAKGIEKFRKAGQAVQGNTLSGDDAFILWDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNKQAGGAIVMDADATSTLHKAGVSATDDSFKYIWFQDHESELKAIYT-GSTFLESSAASDNVGLV--LGSTSFYAEQGGQ 585

[Close]

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVF ELK+ E  I ++       F + L+ G  K         G       +  +      P+           +      FN             QNKQAGGAIVMDADATS LHK  ++ TDDSFK++WF+DHES +KAIYT GS F      +D    V       FYAEQGGQ
Sbjct:  362 MGDVFTELKEHEKKITDIIKEEEASFCKTLAKGIEKFRKAGQAVQGNTLSGDDAFILWDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNKQAGGAIVMDADATSTLHKAGVSATDDSFKYIWFQDHESELKAIYT-GSTFLESSAASDNVGLV--LGSTSFYAEQGGQ 537

[Close]

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVF ELK+ E  I ++       F + L+ G  K         G       +  +      P+           +      FN             QNKQAGGAIVMDADATS LHK  ++ TDDSFK++WF+DHES +KAIYT GS F      +D    V       FYAEQGGQ
Sbjct:  410 MGDVFTELKEHEKKITDIIKEEEASFCKTLAKGIEKFRKAGQAVQGNTLSGDDAFILWDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNKQAGGAIVMDADATSTLHKAGVSATDDSFKYIWFQDHESELKAIYT-GSTFLESSAASDNVGLV--LGSTSFYAEQGGQ 585

[Close]

BLAST of AM161865 vs. TrEMBL
Match: D7T9F5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00012030001 PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 9.153e-29
Identity = 53/81 (65.43%), Postives = 59/81 (72.84%), Query Frame = 2

Query:  296 QNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            QNKQAGG IVMDADATSALHKR +APTDDSFKF WF+DH SV+KAIY+ GSEF  +   + G           FYAEQGGQ
Sbjct:  531 QNKQAGGTIVMDADATSALHKRGVAPTDDSFKFTWFQDHGSVIKAIYS-GSEF--LESASAGNEVGIVLETTSFYAEQGGQ 608

HSP 2 Score: 53.1434 bits (126), Expect = 9.153e-29
Identity = 24/30 (80.00%), Postives = 27/30 (90.00%), Query Frame = 3

Query:  162 AFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            AF LWDT+GFPLDLTQLMAEE+ L VDV+G
Sbjct:  486 AFVLWDTYGFPLDLTQLMAEERGLIVDVQG 515

[Close]

BLAST of AM161865 vs. TrEMBL
Match: B9H602_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_558710 PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.970e-24
Identity = 81/181 (44.75%), Postives = 97/181 (53.59%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTE-RGGSV*VVGYIRVPIRSDTAY-G*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            + DVFPELK+ E HIR   +     F + L  G  K         GK    +   V +VGYI +PIR D+   G R  +      FN             QNKQAGGAIVMDADATSALHK+ ++ TDDSFKF+ F+DHESV+KAIY  GSEF       +    V       FYAEQGGQ
Sbjct:  415 MSDVFPELKRNEEHIRETIAAEEASFGKTLLKGIEKFKKAAQEVEGKVLSGQARCVCLVGYIWLPIRFDSEERGLRVDVEG----FNNAMDEARERSRNAQNKQAGGAIVMDADATSALHKKGVSATDDSFKFICFEDHESVIKAIYN-GSEFLESAAAANEVGIV--LESTSFYAEQGGQ 588

[Close]

BLAST of AM161865 vs. TrEMBL
Match: D7T7J0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_39.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024320001 PE=3 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.561e-21
Identity = 72/179 (40.22%), Postives = 87/179 (48.60%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVFPELKQ E+HIR + +     F + L  G  K         GK      +  +      P+           +T     FN             QNK+AGGAIVMDADAT+ALHK  +A TDD  KF WF+DHESV+KAIYT G EF       D    +       FYAEQGGQ
Sbjct:  367 MGDVFPELKQHEMHIRGIIAAEEASFGKTLIKGIEKFKKAAQEVQGKILSGQEAFVLWDTYGFPLDLTQLMAEERGLTVDVMGFNIAMDEARERSRNAQNKKAGGAIVMDADATAALHKMGVAATDDISKFTWFEDHESVIKAIYT-GIEFLESAAAGDEVGII--LETTSFYAEQGGQ 542

[Close]

BLAST of AM161865 vs. TrEMBL
Match: B9SYR5_RICCO (Alanyl-tRNA synthetase, putative OS=Ricinus communis GN=RCOM_0036070 PE=3 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 7.747e-21
Identity = 70/179 (39.11%), Postives = 88/179 (49.16%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVFPEL Q EV IR +       F + L  G  K         GK +    +  +      P+           +      FN             QNKQAGG I+MDADATSALHK+ ++ TDDS+KF+WF+DHESV+KAIYT G+EF  +   T             FYAEQGGQ
Sbjct:  432 MGDVFPELIQNEVRIREIIKEEEASFGKTLLKGIEKFKKAAQEVQGKVSCLQDAFVLWDTYGFPLDLTQLMAEERGLWVDVEGFNNAMDEARERSRNAQNKQAGGDIIMDADATSALHKKGVSVTDDSYKFIWFQDHESVIKAIYT-GTEF--LGSTTSSNEVGIVLESTSFYAEQGGQ 607

[Close]

BLAST of AM161865 vs. TrEMBL
Match: D7KFW7_ARALY (Cytosolic tRNA-Ala synthetase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_474152 PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.119e-19
Identity = 69/179 (38.55%), Postives = 84/179 (46.93%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVF ELK+ E  I  +       F + L  G  K         G       +  +      P+          ++      FN             QNKQAGGAIVMDADATS LHK  ++ TDDSFK++WF+DHES +KAIYT GS F   L  TD    V       FYAEQGGQ
Sbjct:  362 MGDVFTELKEHEKKITEIIKEEEASFCKTLVKGIEKFQKAEQAVQGNTLSGEDAFVLWDTYGFPLDLTQLMAEERRLLVDVEGFNKAMEEARERSRSAQNKQAGGAIVMDADATSTLHKTGVSATDDSFKYIWFQDHESELKAIYT-GSTFLESLAATDNVGLV--LGSTSFYAEQGGQ 537

[Close]

BLAST of AM161865 vs. TrEMBL
Match: D8M1P4_BLAHO (Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_16 OS=Blastocystis hominis GN=GSBLH_T00002066001 PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 3.631e-15
Identity = 34/76 (44.74%), Postives = 51/76 (67.11%), Query Frame = 3

Query:   33 RKCTLEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGN---TLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            + C ++  + +EE SF RTL KG+ +F    +  Q +    ++G+VAF L+ T GFP+DLT+LMAEE  L+VD+EG
Sbjct:  370 KMCFVQEVILDEELSFNRTLEKGLKRFAQEAEACQASGAKVITGDVAFFLYGTLGFPIDLTELMAEEAGLKVDMEG 445

HSP 2 Score: 38.891 bits (89), Expect = 3.631e-15
Identity = 29/83 (34.94%), Postives = 37/83 (44.58%), Query Frame = 2

Query:  299 NKQAGG---AIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            NK+ G     +VM+A+  S L K  +  TDD  K+ WF+  ES V A Y     F   +    G   V       FYAE  GQ
Sbjct:  460 NKKNGAEQTTLVMEAEQVSHLQKSGVPVTDDLPKYTWFETLESEVVAFYGADKSFPEQIAPESGVVGV-VLKQTPFYAESEGQ 541

[Close]

BLAST of AM161865 vs. TrEMBL
Match: A9T4K7_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_140193 PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.083e-14
Identity = 41/65 (63.08%), Postives = 47/65 (72.31%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            + EEE SF RTLVKGI KFK A   V+   L  + AF LWDTFGFP+DLT+LMAEE  L+VD EG
Sbjct:  376 ISEEESSFSRTLVKGIEKFKKAAAEVKDGVLGAQDAFILWDTFGFPIDLTELMAEEMGLKVDKEG 440

HSP 2 Score: 57.7658 bits (138), Expect = 6.367e-7
Identity = 51/186 (27.42%), Postives = 73/186 (39.25%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYT-------PGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +G+ FPELK+ +  I+ + S     F + L  G  K     +           +  +      PI           +   +  FN             + K  G A+VM+A+ATS L K  I PTDD  K++W K+    VKAI+T          E   I+L T             +YAEQGGQ
Sbjct:  358 MGEAFPELKKNQAKIKEIISEEESSFSRTLVKGIEKFKKAAAEVKDGVLGAQDAFILWDTFGFPIDLTELMAEEMGLKVDKEGFNRTMEEAREKARGARGKAGGKALVMEAEATSQLQKLGILPTDDKPKYIWHKNPTVTVKAIFTSSGFEESTSDEDVGIVLDT-----------TSYYAEQGGQ 532

[Close]

BLAST of AM161865 vs. TrEMBL
Match: Q583T3_9TRYP (Alanyl-tRNA synthetase, putative OS=Trypanosoma brucei GN=Tb927.6.700 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.833e-14
Identity = 33/74 (44.59%), Postives = 43/74 (58.11%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQE 278
            L +EE SF RT   G+  F  AV     N +SGE AF L D +GFP+DLT L+AE+  + VD+EG     K  +
Sbjct:  378 LADEETSFARTWETGLKHFNKAVAEAVNNVISGENAFILHDRYGFPVDLTSLLAEKANMSVDLEGFHATMKASQ 451

HSP 2 Score: 36.965 bits (84), Expect = 3.833e-14
Identity = 26/81 (32.10%), Postives = 37/81 (45.68%), Query Frame = 2

Query:  308 AGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV----*FWNLQDFYAEQGGQ 538
            A     +D      L    ++PTDDS K+ W K H + VKAI+   S     +L+ D            ++ +FYAE GGQ
Sbjct:  458 AAAKTFIDVHQLEELKAGGVSPTDDSAKYTW-KKHVAEVKAIFDKKSGSFVDVLLPDSEMGPEDIGIILDVTNFYAEAGGQ 537

[Close]

BLAST of AM161865 vs. TrEMBL
Match: C9ZQ79_TRYBG (Alanyl-tRNA synthetase, putative OS=Trypanosoma brucei gambiense DAL972 GN=TbgDal_VI390 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.833e-14
Identity = 33/74 (44.59%), Postives = 43/74 (58.11%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQE 278
            L +EE SF RT   G+  F  AV     N +SGE AF L D +GFP+DLT L+AE+  + VD+EG     K  +
Sbjct:  378 LADEETSFARTWETGLKHFNKAVAEAVNNVISGENAFILHDRYGFPVDLTSLLAEKANMSVDLEGFHATMKASQ 451

HSP 2 Score: 36.965 bits (84), Expect = 3.833e-14
Identity = 26/81 (32.10%), Postives = 37/81 (45.68%), Query Frame = 2

Query:  308 AGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV----*FWNLQDFYAEQGGQ 538
            A     +D      L    ++PTDDS K+ W K H + VKAI+   S     +L+ D            ++ +FYAE GGQ
Sbjct:  458 AAAKTFIDVHQLEELKAGGVSPTDDSAKYTW-KKHVAEVKAIFDKKSGSFVDVLLPDSEMGPEDIGIILDVTNFYAEAGGQ 537

[Close]

BLAST of AM161865 vs. TrEMBL
Match: C1EDP7_MICSR (Alanyl-tRNA synthetase OS=Micromonas sp. (strain RCC299 / NOUM17) GN=MICPUN_62346 PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 4.983e-14
Identity = 37/81 (45.68%), Postives = 53/81 (65.43%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQG---NTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWK-LQEKGQ 287
            + EEEESFGRTL+KG+ +FK      +    +T+SG  AF LW++FGFP+DL ++M EE  ++VD  G    +K  QEK +
Sbjct:  376 IAEEEESFGRTLLKGVEQFKKIAAKAKEEGRDTVSGAEAFLLWESFGFPVDLVEIMCEENGMKVDNAGFEEAFKEAQEKSR 456

HSP 2 Score: 32.3426 bits (72), Expect = 4.983e-14
Identity = 26/79 (32.91%), Postives = 37/79 (46.84%), Query Frame = 2

Query:  302 KQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            K  G  ++ +A+AT+ L    +  TDDS K+      E+ VKAI T    F       +G   +   +   FYAE GGQ
Sbjct:  461 KGGGRQLLFEAEATAWLANNGVPLTDDSHKYAAGSVPEATVKAILTLDG-FVESTENVEGPIGL-VLDKTSFYAESGGQ 537

[Close]

BLAST of AM161865 vs. Lotus protein
Match: LjSGA_130854.1 (- phase: 0 /partial)

HSP 1 Score: 68.5514 bits (166), Expect = 1.874e-23
Identity = 31/36 (86.11%), Postives = 33/36 (91.67%), Query Frame = 2

Query:  296 QNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWF 403
            Q KQAGGAIVMDADATSALHK+ I PTDDSFK+VWF
Sbjct:   47 QTKQAGGAIVMDADATSALHKKGILPTDDSFKYVWF 82

HSP 2 Score: 56.9954 bits (136), Expect = 1.874e-23
Identity = 27/30 (90.00%), Postives = 27/30 (90.00%), Query Frame = 3

Query:  162 AFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            AF LWDTFGFPLDLTQLMAEEK L VDVEG
Sbjct:    2 AFVLWDTFGFPLDLTQLMAEEKGLLVDVEG 31

[Close]

BLAST of AM161865 vs. Lotus protein
Match: LjSGA_068125.1 (- phase: 0 /pseudo/partial)

HSP 1 Score: 56.9954 bits (136), Expect = 3.711e-18
Identity = 27/30 (90.00%), Postives = 27/30 (90.00%), Query Frame = 3

Query:  162 AFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            AF LWDTFGFPLDLTQLMAEEK L VDVEG
Sbjct:    2 AFVLWDTFGFPLDLTQLMAEEKGLLVDVEG 31

HSP 2 Score: 45.0542 bits (105), Expect = 3.711e-18
Identity = 21/26 (80.77%), Postives = 23/26 (88.46%), Query Frame = 2

Query:  296 QNKQAGGAIVMDADATSALHKRSIAP 373
            Q KQAGGAIVMDADATSALHK+  +P
Sbjct:   47 QTKQAGGAIVMDADATSALHKKGNSP 72

HSP 3 Score: 25.409 bits (54), Expect = 3.711e-18
Identity = 11/12 (91.67%), Postives = 11/12 (91.67%), Query Frame = 1

Query:  373 NR*QFQICLVQG 408
            NR*Q QICLVQG
Sbjct:   73 NR*QLQICLVQG 84

[Close]

BLAST of AM161865 vs. Lotus protein
Match: LjSGA_020303.0.1 (- phase: 1 /partial)

HSP 1 Score: 78.9518 bits (193), Expect = 1.077e-15
Identity = 39/62 (62.90%), Postives = 47/62 (75.81%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEV----------AFELWDTFGFPLDLTQL 212
            ++EEEESFGRTL+KGI KF+++V+HVQG  LSGE           AF LWDTFGFPLDLTQ+
Sbjct:   38 IKEEEESFGRTLIKGIEKFESSVRHVQGKLLSGEASRICIFSATEAFVLWDTFGFPLDLTQV 99

[Close]

BLAST of AM161865 vs. Soybean peptides
Match: Glyma12g03140.1|PACid:16286196 ()

HSP 1 Score: 131.339 bits (329), Expect = 4.578e-31
Identity = 91/197 (46.19%), Postives = 103/197 (52.28%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERG------------------GSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVFPELKQQE HIRNV       F + L  G  K         GK                       GS+ +VGYIRV IRS +  G    +    + F+             Q KQAGGAIVM+ADATSALHKR I PTDDSFK+ WFKDHESVVKAIYT GSEF   +  +D    V       FYAEQGGQ
Sbjct:  362 MGDVFPELKQQETHIRNVIEEEEESFGRTLIKGIEKFETAVRHVQGKILSGEAIAHKVAVKSDSYCLLVIGSICLVGYIRVSIRSHSEKG----LVVDFKGFDSAMEAARERSRSAQTKQAGGAIVMEADATSALHKRGIVPTDDSFKYAWFKDHESVVKAIYT-GSEFVDTVNTSDDIGVV--LESTSFYAEQGGQ 551

[Close]

BLAST of AM161865 vs. Soybean peptides
Match: Glyma11g10860.1|PACid:16283413 ()

HSP 1 Score: 116.316 bits (290), Expect = 1.524e-26
Identity = 77/179 (43.02%), Postives = 91/179 (50.84%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVFPELKQQE HIRN+       F + L  G  K         G       +  +      P+         + +    + F+             Q KQAGGAIVMDADATSALHKR I PT+DSFK+ WFKDHESVVKAIYT GSEF  +  +  G           FYAEQGGQ
Sbjct:  362 MGDVFPELKQQETHIRNIIEEEEESFGRTLIKGIEKFEAAVRQVQGNILSGEEAFVLWDTYGFPLDLTQLMAEEKGLVVDVKGFDSAMEAARERSRSAQTKQAGGAIVMDADATSALHKRGIVPTNDSFKYAWFKDHESVVKAIYT-GSEF--VDTVNTGDDIGVVLESTSFYAEQGGQ 537

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_ARATH (Alanyl-tRNA synthetase OS=Arabidopsis thaliana GN=ALATS PE=1 SV=3)

HSP 1 Score: 96.2857 bits (238), Expect = 1.020e-19
Identity = 67/179 (37.43%), Postives = 84/179 (46.93%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            +GDVF ELK+ E  I ++       F + L+ G  K         G       +  +      P+           +      FN             QNKQAGGAIVMDADATS LHK  ++ TDDSFK++WF+DHES +KAIYT GS F      +D    V       FYAEQGGQ
Sbjct:  410 MGDVFTELKEHEKKITDIIKEEEASFCKTLAKGIEKFRKAGQAVQGNTLSGDDAFILWDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEEARERSRSAQNKQAGGAIVMDADATSTLHKAGVSATDDSFKYIWFQDHESELKAIYT-GSTFLESSAASDNVGLV--LGSTSFYAEQGGQ 585

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYAC_DICDI (Alanyl-tRNA synthetase, cytoplasmic OS=Dictyostelium discoideum GN=alaS PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.273e-17
Identity = 34/79 (43.04%), Postives = 51/79 (64.56%), Query Frame = 3

Query:   45 LEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            ++M L  EE+ F +TL KGI +F+  ++    NTLS E A+ L   +GFP+DLT +MAEEK  +VD++G   L + Q +
Sbjct:  368 IKMVLTREEDMFNKTLEKGIVEFEKMIKKSVNNTLSAENAYFLSTCYGFPIDLTTIMAEEKNYKVDIKGYEGLCEAQSE 446

HSP 2 Score: 42.743 bits (99), Expect = 1.273e-17
Identity = 32/83 (38.55%), Postives = 43/83 (51.81%), Query Frame = 2

Query:  296 QNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHE--SVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            + K+    + + A+A + L    I PTDDSFK   +K HE  SV+KAI+  G EF  +     G          +FYAEQGGQ
Sbjct:  451 RQKEKKVELTLGAEANAWLKNNDIKPTDDSFK---YKQHEIQSVIKAIWN-GKEF--VDTAPKGTLIGVVLENSNFYAEQGGQ 527

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_ACTPJ (Alanyl-tRNA synthetase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.419e-13
Identity = 35/65 (53.85%), Postives = 48/65 (73.85%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            L+ EEE F RTL +G+A  + A+  V+ NTLSGEVAF+L+DT+GFPLDLT  +  E++L +D  G
Sbjct:  359 LKAEEEQFARTLERGLALLEDALTKVENNTLSGEVAFKLYDTYGFPLDLTADVCRERELTIDEAG 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_ACTP2 (Alanyl-tRNA synthetase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.419e-13
Identity = 35/65 (53.85%), Postives = 48/65 (73.85%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            L+ EEE F RTL +G+A  + A+  V+ NTLSGEVAF+L+DT+GFPLDLT  +  E++L +D  G
Sbjct:  359 LKAEEEQFARTLERGLALLEDALTKVENNTLSGEVAFKLYDTYGFPLDLTADVCRERELTIDEAG 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_LEGPH (Alanyl-tRNA synthetase OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.243e-13
Identity = 36/84 (42.86%), Postives = 55/84 (65.48%), Query Frame = 3

Query:   30 NRKCTLEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            N K  +E  LQ+EE  F RTL +G+   +  ++++QG  LSGEVAF+L+DT+GFP+DLT  +  E+ L +D+E    L + Q +
Sbjct:  340 NSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYGFPIDLTADIIREQGLHIDMEAFNQLMQQQRE 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_LEGPC (Alanyl-tRNA synthetase OS=Legionella pneumophila (strain Corby) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.243e-13
Identity = 36/84 (42.86%), Postives = 55/84 (65.48%), Query Frame = 3

Query:   30 NRKCTLEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            N K  +E  LQ+EE  F RTL +G+   +  ++++QG  LSGEVAF+L+DT+GFP+DLT  +  E+ L +D+E    L + Q +
Sbjct:  340 NSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYGFPIDLTADIIREQGLHIDMEAFNQLMQQQRE 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_ACTP7 (Alanyl-tRNA synthetase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=alaS PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.243e-13
Identity = 34/65 (52.31%), Postives = 48/65 (73.85%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            L+ EEE F RTL +G+A  + A+  V+ NTLSGEVAF+L+DT+GFPLDLT  +  E+++ +D  G
Sbjct:  359 LKAEEEQFARTLERGLALLEDALTKVENNTLSGEVAFKLYDTYGFPLDLTADVCREREITIDEAG 423

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_FRATW (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. tularensis (strain WY96-3418) GN=alaS PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.577e-12
Identity = 35/79 (44.30%), Postives = 55/79 (69.62%), Query Frame = 3

Query:   45 LEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            +E  L +EEE F +T+  GI  F   +++++ NT+SGEVAF+L+DT+GFP DLT  MA EK L+VD +  +   ++Q++
Sbjct:  346 IEKTLIKEEELFLKTIENGIKIFDAEIENLKDNTISGEVAFKLYDTYGFPFDLTADMAREKGLKVDEQAFLAQMQIQKQ 424

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_FRATT (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. tularensis GN=alaS PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.577e-12
Identity = 35/79 (44.30%), Postives = 55/79 (69.62%), Query Frame = 3

Query:   45 LEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            +E  L +EEE F +T+  GI  F   +++++ NT+SGEVAF+L+DT+GFP DLT  MA EK L+VD +  +   ++Q++
Sbjct:  346 IEKTLIKEEELFLKTIENGIKIFDAEIENLKDNTISGEVAFKLYDTYGFPFDLTADMAREKGLKVDEQAFLAQMQIQKQ 424

[Close]

BLAST of AM161865 vs. SwissProt
Match: SYA_FRATO (Alanyl-tRNA synthetase OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=alaS PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.577e-12
Identity = 35/79 (44.30%), Postives = 55/79 (69.62%), Query Frame = 3

Query:   45 LEM*LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEGLIVLWKLQEK 281
            +E  L +EEE F +T+  GI  F   +++++ NT+SGEVAF+L+DT+GFP DLT  MA EK L+VD +  +   ++Q++
Sbjct:  346 IEKTLIKEEELFLKTIENGIKIFDAEIENLKDNTISGEVAFKLYDTYGFPFDLTADMAREKGLKVDEQAFLAQMQIQKQ 424

[Close]

BLAST of AM161865 vs. Medicago proteins
Match: IMGA|Medtr4g074640.1 (Alanyl-tRNA synthetase, mitochondrial (AHRD V1 **** P36428); contains Interpro domain(s) IPR002318 Alanyl-tRNA synthetase, class IIc chr04_pseudomolecule_IMGAG_V3.5 23711320-23725867 E EGN_Mt100125 20100825)

HSP 1 Score: 123.25 bits (308), Expect = 7.273e-29
Identity = 60/65 (92.31%), Postives = 63/65 (96.92%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            +QEEEESFGRTLVKGIAKFKTAV HVQGN LSGEVAFELWDT+GFPLDLTQLMAEEKKL+VDVEG
Sbjct:  380 IQEEEESFGRTLVKGIAKFKTAVGHVQGNILSGEVAFELWDTYGFPLDLTQLMAEEKKLKVDVEG 444

HSP 2 Score: 122.479 bits (306), Expect = 1.241e-28
Identity = 85/179 (47.49%), Postives = 94/179 (52.51%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            LGDVFPELKQQEVHIRNV       F + L  G  K         G       +  +      P+         +K+      FN             QNKQAGGAIVMDADATSAL KRSI PTDDSFKF  F+DHE+VVKAIYT GSEF    + TDG   V       FYAEQGGQ
Sbjct:  362 LGDVFPELKQQEVHIRNVIQEEEESFGRTLVKGIAKFKTAVGHVQGNILSGEVAFELWDTYGFPLDLTQLMAEEKKLKVDVEGFNSAMEAARERSRTAQNKQAGGAIVMDADATSALQKRSIVPTDDSFKFASFRDHETVVKAIYT-GSEFV-DSVNTDGDVGV-ILESTSFYAEQGGQ 537

[Close]

BLAST of AM161865 vs. Medicago proteins
Match: IMGA|Medtr6g045600.1 (Alanyl-tRNA synthetase, mitochondrial (AHRD V1 **** P36428); contains Interpro domain(s) IPR002318 Alanyl-tRNA synthetase, class IIc chr06_pseudomolecule_IMGAG_V3.5 9122747-9109637 E EGN_Mt100125 20100825)

HSP 1 Score: 123.25 bits (308), Expect = 7.273e-29
Identity = 60/65 (92.31%), Postives = 63/65 (96.92%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            +QEEEESFGRTLVKGIAKFKTAV HVQGN LSGEVAFELWDT+GFPLDLTQLMAEEKKL+VDVEG
Sbjct:  380 IQEEEESFGRTLVKGIAKFKTAVGHVQGNILSGEVAFELWDTYGFPLDLTQLMAEEKKLKVDVEG 444

HSP 2 Score: 122.479 bits (306), Expect = 1.241e-28
Identity = 85/179 (47.49%), Postives = 94/179 (52.51%), Query Frame = 2

Query:    2 LGDVFPELKQQEVHIRNVTSRGRGKFWQNLS*GHCKI*DCCSTRPGKHTERGGSV*VVGYIRVPIRSDTAYG*REKITS*RRRFNXXXXXXXXXXXXXQNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            LGDVFPELKQQEVHIRNV       F + L  G  K         G       +  +      P+         +K+      FN             QNKQAGGAIVMDADATSAL KRSI PTDDSFKF  F+DHE+VVKAIYT GSEF    + TDG   V       FYAEQGGQ
Sbjct:  362 LGDVFPELKQQEVHIRNVIQEEEESFGRTLVKGIAKFKTAVGHVQGNILSGEVAFELWDTYGFPLDLTQLMAEEKKLKVDVEGFNSAMEAARERSRTAQNKQAGGAIVMDADATSALQKRSIVPTDDSFKFASFRDHETVVKAIYT-GSEFV-DSVNTDGDVGV-ILESTSFYAEQGGQ 537

[Close]

BLAST of AM161865 vs. Medicago proteins
Match: IMGA|Medtr4g074670.2 (Alanyl-tRNA synthetase (AHRD V1 ***- D2VJ86_NAEGR); contains Interpro domain(s) IPR018164 Alanyl-tRNA synthetase, class IIc, N-terminal chr04_pseudomolecule_IMGAG_V3.5 23733334-23740196 E EGN_Mt100125 20100825)

HSP 1 Score: 100.523 bits (249), Expect = 5.049e-22
Identity = 50/65 (76.92%), Postives = 53/65 (81.54%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            +Q EEESF R L KGI KF  AV+HVQGN LSGEVAFEL  TFGFPLDLTQLMAEEKK  VD+EG
Sbjct:  134 IQVEEESFSRNLFKGIRKFNKAVEHVQGNILSGEVAFELSHTFGFPLDLTQLMAEEKKFSVDIEG 198

[Close]

BLAST of AM161865 vs. Medicago proteins
Match: IMGA|Medtr4g074670.1 (Alanyl-tRNA synthetase (Fragment) (AHRD V1 *-** C9D9A7_BLAGE); contains Interpro domain(s) IPR018164 Alanyl-tRNA synthetase, class IIc, N-terminal chr04_pseudomolecule_IMGAG_V3.5 23732393-23740196 E EGN_Mt100125 20100825)

HSP 1 Score: 100.523 bits (249), Expect = 5.049e-22
Identity = 50/65 (76.92%), Postives = 53/65 (81.54%), Query Frame = 3

Query:   57 LQEEEESFGRTLVKGIAKFKTAVQHVQGNTLSGEVAFELWDTFGFPLDLTQLMAEEKKLQVDVEG 251
            +Q EEESF R L KGI KF  AV+HVQGN LSGEVAFEL  TFGFPLDLTQLMAEEKK  VD+EG
Sbjct:  380 IQVEEESFSRNLFKGIRKFNKAVEHVQGNILSGEVAFELSHTFGFPLDLTQLMAEEKKFSVDIEG 444

[Close]

BLAST of AM161865 vs. Medicago proteins
Match: IMGA|Medtr6g045600.2 (Alanyl-tRNA synthetase, mitochondrial (AHRD V1 ***- P36428); contains Interpro domain(s) IPR018163 Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain chr06_pseudomolecule_IMGAG_V3.5 9119363-9109637 E EGN_Mt100125 20100825)

HSP 1 Score: 98.9821 bits (245), Expect = 1.469e-21
Identity = 58/81 (71.60%), Postives = 61/81 (75.31%), Query Frame = 2

Query:  296 QNKQAGGAIVMDADATSALHKRSIAPTDDSFKFVWFKDHESVVKAIYTPGSEFC*ILLITDGRCXV*FWNLQDFYAEQGGQ 538
            QNKQAGGAIVMDADATSAL KRSI PTDDSFKF  F+DHE+VVKAIYT GSEF    + TDG   V       FYAEQGGQ
Sbjct:   12 QNKQAGGAIVMDADATSALQKRSIVPTDDSFKFASFRDHETVVKAIYT-GSEFV-DSVNTDGDVGV-ILESTSFYAEQGGQ 89

The following BLAST results are available for this feature:

BLAST of AM161865 vs. TrEMBL
BLAST of AM161865 vs. SwissProt
BLAST of AM161865 vs. TAIR peptide
BLAST of AM161865 vs. TAIR peptide
BLAST of AM161865 vs. TrEMBL
BLAST of AM161865 vs. Lotus protein
BLAST of AM161865 vs. Soybean peptides
BLAST of AM161865 vs. SwissProt
BLAST of AM161865 vs. Medicago proteins

BLAST of AM161865 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7T9F5_VITVI	7.503e-29	65.43	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9H602_POPTR	1.615e-24	44.75	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7T7J0_VITVI	1.280e-21	40.22	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SYR5_RICCO	6.352e-21	39.11	Alanyl-tRNA synthetase, putative OS=Ricinus commun...	[more]
D7KFW7_ARALY	9.172e-20	38.55	Cytosolic tRNA-Ala synthetase OS=Arabidopsis lyrat...	[more]
A9T4K7_PHYPA	8.884e-15	63.08	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
Q583T3_9TRYP	3.142e-14	44.59	Alanyl-tRNA synthetase, putative OS=Trypanosoma br...	[more]
C9ZQ79_TRYBG	3.142e-14	44.59	Alanyl-tRNA synthetase, putative OS=Trypanosoma br...	[more]
C1EDP7_9CHLO	4.084e-14	45.68	Alanyl-tRNA synthetase OS=Micromonas sp. RCC299 GN...	[more]
D3B7Q7_POLPA	5.304e-14	44.93	Alanyl-tRNA synthetase OS=Polysphondylium pallidum...	[more]

BLAST of AM161865 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SYA_ARATH	1.001e-19	37.43	Alanyl-tRNA synthetase, mitochondrial OS=Arabidops...	[more]
SYA_DICDI	1.251e-17	43.04	Alanyl-tRNA synthetase OS=Dictyostelium discoideum...	[more]
SYA_ACTPJ	5.322e-13	53.85	Alanyl-tRNA synthetase OS=Actinobacillus pleuropne...	[more]
SYA_ACTP2	5.322e-13	53.85	Alanyl-tRNA synthetase OS=Actinobacillus pleuropne...	[more]
SYA_LEGPH	9.078e-13	42.86	Alanyl-tRNA synthetase OS=Legionella pneumophila s...	[more]
SYA_LEGPC	9.078e-13	42.86	Alanyl-tRNA synthetase OS=Legionella pneumophila (...	[more]
SYA_ACTP7	9.078e-13	52.31	Alanyl-tRNA synthetase OS=Actinobacillus pleuropne...	[more]
SYA_FRATW	1.548e-12	44.30	Alanyl-tRNA synthetase OS=Francisella tularensis s...	[more]
SYA_FRATT	1.548e-12	44.30	Alanyl-tRNA synthetase OS=Francisella tularensis s...	[more]
SYA_FRATO	1.548e-12	44.30	Alanyl-tRNA synthetase OS=Francisella tularensis s...	[more]

BLAST of AM161865 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G50200.2	1.091e-20	37.43	\| Symbols: ALATS \| Alanyl-tRNA synthetase \| chr1:1...	[more]
AT1G50200.1	1.091e-20	37.43	\| Symbols: ALATS, ACD \| Alanyl-tRNA synthetase \| c...	[more]

BLAST of AM161865 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G50200.2	1.091e-20	37.43	\| Symbols: ALATS \| Alanyl-tRNA synthetase \| chr1:1...	[more]
AT1G50200.1	1.091e-20	37.43	\| Symbols: ALATS, ACD \| Alanyl-tRNA synthetase \| c...	[more]

BLAST of AM161865 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7T9F5_VITVI	9.153e-29	65.43	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9H602_POPTR	1.970e-24	44.75	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7T7J0_VITVI	1.561e-21	40.22	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SYR5_RICCO	7.747e-21	39.11	Alanyl-tRNA synthetase, putative OS=Ricinus commun...	[more]
D7KFW7_ARALY	1.119e-19	38.55	Cytosolic tRNA-Ala synthetase OS=Arabidopsis lyrat...	[more]
D8M1P4_BLAHO	3.631e-15	44.74	Singapore isolate B (sub-type 7) whole genome shot...	[more]
A9T4K7_PHYPA	1.083e-14	63.08	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
Q583T3_9TRYP	3.833e-14	44.59	Alanyl-tRNA synthetase, putative OS=Trypanosoma br...	[more]
C9ZQ79_TRYBG	3.833e-14	44.59	Alanyl-tRNA synthetase, putative OS=Trypanosoma br...	[more]
C1EDP7_MICSR	4.983e-14	45.68	Alanyl-tRNA synthetase OS=Micromonas sp. (strain R...	[more]

BLAST of AM161865 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 3


Match Name	E-value	Identity	Description
LjSGA_130854.1	1.874e-23	86.11	- phase: 0 /partial	[more]
LjSGA_068125.1	3.711e-18	90.00	- phase: 0 /pseudo/partial	[more]
LjSGA_020303.0.1	1.077e-15	62.90	- phase: 1 /partial	[more]

BLAST of AM161865 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 2


Match Name	E-value	Identity	Description
Glyma12g03140.1\|PACid:16286196	4.578e-31	46.19		[more]
Glyma11g10860.1\|PACid:16283413	1.524e-26	43.02		[more]

BLAST of AM161865 vs. SwissProt
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SYA_ARATH	1.020e-19	37.43	Alanyl-tRNA synthetase OS=Arabidopsis thaliana GN=...	[more]
SYAC_DICDI	1.273e-17	43.04	Alanyl-tRNA synthetase, cytoplasmic OS=Dictyosteli...	[more]
SYA_ACTPJ	5.419e-13	53.85	Alanyl-tRNA synthetase OS=Actinobacillus pleuropne...	[more]
SYA_ACTP2	5.419e-13	53.85	Alanyl-tRNA synthetase OS=Actinobacillus pleuropne...	[more]
SYA_LEGPH	9.243e-13	42.86	Alanyl-tRNA synthetase OS=Legionella pneumophila s...	[more]
SYA_LEGPC	9.243e-13	42.86	Alanyl-tRNA synthetase OS=Legionella pneumophila (...	[more]
SYA_ACTP7	9.243e-13	52.31	Alanyl-tRNA synthetase OS=Actinobacillus pleuropne...	[more]
SYA_FRATW	1.577e-12	44.30	Alanyl-tRNA synthetase OS=Francisella tularensis s...	[more]
SYA_FRATT	1.577e-12	44.30	Alanyl-tRNA synthetase OS=Francisella tularensis s...	[more]
SYA_FRATO	1.577e-12	44.30	Alanyl-tRNA synthetase OS=Francisella tularensis s...	[more]

BLAST of AM161865 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 5


Match Name	E-value	Identity	Description
IMGA\|Medtr4g074640.1	7.273e-29	92.31	Alanyl-tRNA synthetase, mitochondrial (AHRD V1 ***...	[more]
IMGA\|Medtr6g045600.1	7.273e-29	92.31	Alanyl-tRNA synthetase, mitochondrial (AHRD V1 ***...	[more]
IMGA\|Medtr4g074670.2	5.049e-22	76.92	Alanyl-tRNA synthetase (AHRD V1 ***- D2VJ86_NAEGR)...	[more]
IMGA\|Medtr4g074670.1	5.049e-22	76.92	Alanyl-tRNA synthetase (Fragment) (AHRD V1 -* C9...	[more]
IMGA\|Medtr6g045600.2	1.469e-21	71.60	Alanyl-tRNA synthetase, mitochondrial (AHRD V1 ***...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 6..18 score
None	No IPR available	PANTHER	PTHR11777	ALANYL-TRNA SYNTHETASE	coord: 1..75 score: 4.5
None	No IPR available	SEG	seg	seg	coord: 79..90 score
IPR018162	Alanyl-tRNA synthetase, class IIc, anti-codon-binding domain	SUPERFAMILY	101353	Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)	coord: 1..111 score: 8.8
IPR018164	Alanyl-tRNA synthetase, class IIc, N-terminal	PFAM	PF01411	tRNA-synt_2c	coord: 1..75 score: 2.7

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 6..18 score
None	No IPR available	PANTHER	PTHR11777	ALANYL-TRNA SYNTHETASE	coord: 1..75 score: 4.5
None	No IPR available	SEG	seg	seg	coord: 79..90 score
IPR018162	Alanyl-tRNA synthetase, class IIc, anti-codon-binding domain	SUPERFAMILY	101353	Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)	coord: 1..111 score: 8.8
IPR018164	Alanyl-tRNA synthetase, class IIc, N-terminal	PFAM	PF01411	tRNA-synt_2c	coord: 1..75 score: 2.7

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v2
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM161865 ID=AM161865; Name=AM161865; organism=Pisum sativum; type=EST; length=540bp
ACTGGGTGATGTTTTCCCTGAGCTAAAACAACAGGAAGTGCACATTAGAA
ATGTGACTTCAAGAGGAAGAGGAAAGTTTTGGCAGAACCTTAGTTAAGGG
CATTGCAAAATTTAAGACTGCTGTTCAACACGTCCAGGGAAACACACTGA
GCGGGGAGGTAGCGTTTGAGTTGTGGGATACATTCGGGTTCCCATTAGAT
CTGACACAGCTTATGGCTGAAGAGAAAAAATTACAAGTTGACGTCGAAGG
TTTAATAGTGCTATGGAAGCTGCAAGAGAAAGGTCAAGAAGTGCTCAAAA
TAAGCAAGCTGGTGGTGCTATTGTCATGGATGCTGATGCTACATCAGCAT
TGCACAAGAGAAGCATTGCTCCAACAGATGACAGTTTCAAATTTGTTTGG
TTCAAGGACCATGAAAGTGTGGTAAAAGCAATATATACACCGGGTTCTGA
GTTTTGTTAGATTCTGTTAATCACTGATGGTCGATGTANGGTGTGATTCT
GGAATCTACAAGACTTCTATGCTGAGCAAGGTGGTCAGAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0004813	alanine-tRNA ligase activity
GO:0005524	ATP binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation
GO:0006419	alanyl-tRNA aminoacylation

Vocabulary: **INTERPRO**
Term	Definition
IPR018162	Ala-tRNA-ligase_IIc_anticod-bd
IPR018164	Ala-tRNA-synth_IIc_N

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