AM161894

EST Overview


Name	AM161894
Unique Name	AM161894
Type	EST
Organism	Pisum sativum (pea)
Sequence length	165

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM161894 vs. TrEMBL
Match: B9RV94_RICCO (Signal recognition particle 54 kD protein, putative OS=Ricinus communis GN=RCOM_0901100 PE=4 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 9.359e-9
Identity = 24/31 (77.42%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVV 311

HSP 2 Score: 33.4982 bits (75), Expect = 9.359e-9
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+G+FTLRIM
Sbjct:  316 QPELLQKLSEGNFTLRIM 333

[Close]

BLAST of AM161894 vs. TrEMBL
Match: A9PHG6_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 9.360e-9
Identity = 24/31 (77.42%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVV 311

HSP 2 Score: 33.4982 bits (75), Expect = 9.360e-9
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+G+FTLRIM
Sbjct:  316 QPELLQKLSEGNFTLRIM 333

[Close]

BLAST of AM161894 vs. TrEMBL
Match: B9GIF9_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_172222 PE=4 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 9.361e-9
Identity = 24/31 (77.42%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVV 311

HSP 2 Score: 33.4982 bits (75), Expect = 9.361e-9
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+G+FTLRIM
Sbjct:  316 QPELLQKLSEGNFTLRIM 333

[Close]

BLAST of AM161894 vs. TrEMBL
Match: B9IG64_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_257619 PE=4 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 9.362e-9
Identity = 24/31 (77.42%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVV 311

HSP 2 Score: 33.4982 bits (75), Expect = 9.362e-9
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+G+FTLRIM
Sbjct:  316 QPELLQKLSEGNFTLRIM 333

[Close]

BLAST of AM161894 vs. TrEMBL
Match: D7U153_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_2.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00017362001 PE=4 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 9.395e-9
Identity = 24/31 (77.42%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVV 311

HSP 2 Score: 33.4982 bits (75), Expect = 9.395e-9
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+G+FTLRIM
Sbjct:  316 QPELLQKLSEGNFTLRIM 333

[Close]

BLAST of AM161894 vs. TrEMBL
Match: C5XLK5_SORBI (Putative uncharacterized protein Sb03g036030 OS=Sorghum bicolor GN=Sb03g036030 PE=4 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 2.650e-8
Identity = 24/31 (77.42%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVV 311

HSP 2 Score: 31.9574 bits (71), Expect = 2.650e-8
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+G FTLR+M
Sbjct:  316 QPELLQKLSEGSFTLRLM 333

[Close]

BLAST of AM161894 vs. TrEMBL
Match: Q8S1I8_ORYSJ (Os01g0772800 protein OS=Oryza sativa subsp. japonica GN=P0695H10.26 PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 3.437e-8
Identity = 24/31 (77.42%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVV 311

HSP 2 Score: 31.5722 bits (70), Expect = 3.437e-8
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+G FTLR+M
Sbjct:  316 QPELLQKLSEGTFTLRLM 333

[Close]

BLAST of AM161894 vs. TrEMBL
Match: B8AA60_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_03915 PE=4 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 3.437e-8
Identity = 24/31 (77.42%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVV 311

HSP 2 Score: 31.5722 bits (70), Expect = 3.437e-8
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+G FTLR+M
Sbjct:  316 QPELLQKLSEGTFTLRLM 333

[Close]

BLAST of AM161894 vs. TrEMBL
Match: B4FSY4_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 5.781e-8
Identity = 23/31 (74.19%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD +GFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWAGFMDKIHEVV 311

HSP 2 Score: 31.9574 bits (71), Expect = 5.781e-8
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+G FTLR+M
Sbjct:  316 QPELLQKLSEGSFTLRLM 333

[Close]

BLAST of AM161894 vs. TrEMBL
Match: C4JC13_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 4.618e-7
Identity = 22/31 (70.97%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            +FEVFDV      LLGMGD SGFMDKIHEV+
Sbjct:  281 DFEVFDVKPFVSRLLGMGDWSGFMDKIHEVI 311

HSP 2 Score: 29.261 bits (64), Expect = 4.618e-7
Identity = 12/18 (66.67%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QP+LLQ LS+G F+LR+M
Sbjct:  316 QPDLLQKLSEGSFSLRLM 333

[Close]

BLAST of AM161894 vs. SwissProt
Match: SR542_SOLLC (Signal recognition particle 54 kDa protein 2 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 7.454e-11
Identity = 24/31 (77.42%), Postives = 24/31 (77.42%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGFMDKIHEVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVV 311

HSP 2 Score: 36.1946 bits (82), Expect = 7.454e-11
Identity = 16/18 (88.89%), Postives = 17/18 (94.44%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+GHFTLRIM
Sbjct:  316 QPELLQKLSEGHFTLRIM 333

[Close]

BLAST of AM161894 vs. SwissProt
Match: SR541_SOLLC (Signal recognition particle 54 kDa protein 1 OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 41.9726 bits (97), Expect = 1.355e-8
Identity = 20/31 (64.52%), Postives = 23/31 (74.19%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGDLSG ++KI +VV
Sbjct:  282 EFEVFDVKPFVSRLLGMGDLSGLVNKIQDVV 312

HSP 2 Score: 36.1946 bits (82), Expect = 1.355e-8
Identity = 16/18 (88.89%), Postives = 17/18 (94.44%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELLQ LS+GHFTLRIM
Sbjct:  317 QPELLQKLSEGHFTLRIM 334

[Close]

BLAST of AM161894 vs. SwissProt
Match: SR543_ARATH (Signal recognition particle 54 kDa protein 3 OS=Arabidopsis thaliana GN=SRP-54C PE=2 SV=2)

HSP 1 Score: 44.2838 bits (103), Expect = 6.426e-8
Identity = 21/31 (67.74%), Postives = 23/31 (74.19%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGF+DK+ EVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFVDKLQEVV 311

HSP 2 Score: 31.5722 bits (70), Expect = 6.426e-8
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELL+ LS G+FTLRIM
Sbjct:  316 QPELLEKLSQGNFTLRIM 333

[Close]

BLAST of AM161894 vs. SwissProt
Match: SR542_ARATH (Signal recognition particle 54 kDa protein 2 OS=Arabidopsis thaliana GN=SRP-54B PE=2 SV=1)

HSP 1 Score: 39.6614 bits (91), Expect = 6.612e-7
Identity = 18/31 (58.06%), Postives = 22/31 (70.97%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFD       LLG GD+SGF++K+ EVV
Sbjct:  281 EFEVFDAKPFVSRLLGNGDMSGFVNKLQEVV 311

HSP 2 Score: 32.7278 bits (73), Expect = 6.612e-7
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELL+MLS G FTLRIM
Sbjct:  316 QPELLEMLSHGKFTLRIM 333

[Close]

BLAST of AM161894 vs. TAIR peptide
Match: AT1G48900.1 (| Symbols: | Signal recognition particle, SRP54 subunit protein | chr1:18084972-18087743 REVERSE LENGTH=495)

HSP 1 Score: 44.2838 bits (103), Expect = 5.136e-9
Identity = 21/31 (67.74%), Postives = 23/31 (74.19%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGF+DK+ EVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFVDKLQEVV 311

HSP 2 Score: 31.5722 bits (70), Expect = 5.136e-9
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELL+ LS G+FTLRIM
Sbjct:  316 QPELLEKLSQGNFTLRIM 333

[Close]

BLAST of AM161894 vs. TAIR peptide
Match: AT1G48900.2 (| Symbols: | Signal recognition particle, SRP54 subunit protein | chr1:18085031-18087743 REVERSE LENGTH=431)

HSP 1 Score: 44.2838 bits (103), Expect = 5.158e-9
Identity = 21/31 (67.74%), Postives = 23/31 (74.19%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFDV      LLGMGD SGF+DK+ EVV
Sbjct:  281 EFEVFDVKPFVSRLLGMGDWSGFVDKLQEVV 311

HSP 2 Score: 31.5722 bits (70), Expect = 5.158e-9
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELL+ LS G+FTLRIM
Sbjct:  316 QPELLEKLSQGNFTLRIM 333

[Close]

BLAST of AM161894 vs. TAIR peptide
Match: AT5G49500.1 (| Symbols: | Signal recognition particle, SRP54 subunit protein | chr5:20077556-20080206 REVERSE LENGTH=497)

HSP 1 Score: 39.6614 bits (91), Expect = 5.284e-8
Identity = 18/31 (58.06%), Postives = 22/31 (70.97%), Query Frame = -2

Query:   66 EFEVFDVXTICQLLLGMGDLSGFMDKIHEVV 158
            EFEVFD       LLG GD+SGF++K+ EVV
Sbjct:  283 EFEVFDAKPFVSRLLGNGDMSGFVNKLQEVV 313

HSP 2 Score: 32.7278 bits (73), Expect = 5.284e-8
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = -3

Query:    2 QPELLQMLSDGHFTLRIM 55
            QPELL+MLS G FTLRIM
Sbjct:  318 QPELLEMLSHGKFTLRIM 335

The following BLAST results are available for this feature:

BLAST of AM161894 vs. TrEMBL
BLAST of AM161894 vs. SwissProt
BLAST of AM161894 vs. TAIR peptide

BLAST of AM161894 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RV94_RICCO	9.359e-9	77.42	Signal recognition particle 54 kD protein, putativ...	[more]
A9PHG6_POPTR	9.360e-9	77.42	Putative uncharacterized protein OS=Populus tricho...	[more]
B9GIF9_POPTR	9.361e-9	77.42	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9IG64_POPTR	9.362e-9	77.42	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7U153_VITVI	9.395e-9	77.42	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5XLK5_SORBI	2.650e-8	77.42	Putative uncharacterized protein Sb03g036030 OS=So...	[more]
Q8S1I8_ORYSJ	3.437e-8	77.42	Os01g0772800 protein OS=Oryza sativa subsp. japoni...	[more]
B8AA60_ORYSI	3.437e-8	77.42	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B4FSY4_MAIZE	5.781e-8	74.19	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C4JC13_MAIZE	4.618e-7	70.97	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of AM161894 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
SR542_SOLLC	7.454e-11	77.42	Signal recognition particle 54 kDa protein 2 OS=So...	[more]
SR541_SOLLC	1.355e-8	64.52	Signal recognition particle 54 kDa protein 1 OS=So...	[more]
SR543_ARATH	6.426e-8	67.74	Signal recognition particle 54 kDa protein 3 OS=Ar...	[more]
SR542_ARATH	6.612e-7	58.06	Signal recognition particle 54 kDa protein 2 OS=Ar...	[more]

BLAST of AM161894 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G48900.1	5.136e-9	67.74	\| Symbols: \| Signal recognition particle, SRP54 s...	[more]
AT1G48900.2	5.158e-9	67.74	\| Symbols: \| Signal recognition particle, SRP54 s...	[more]
AT5G49500.1	5.284e-8	58.06	\| Symbols: \| Signal recognition particle, SRP54 s...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11564	GTPASE CONTAINING FAMILY OF SIGNAL RECOGNITION PARTICLE PROTEINS	coord: 3..33 score: 3.9
None	No IPR available	PANTHER	PTHR11564:SF5	SIGNAL RECOGNITION PARTICLE 54 KDA PROTEIN	coord: 3..33 score: 3.9

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM161894 ID=AM161894; Name=AM161894; organism=Pisum sativum; type=EST; length=165bp
ACATAATCCTCAAGGTAAAGTGTCCATCTGACAGCATTTGAAGCAGTTCG
GGCTGTTATCCATAGAACTACTTCATGGATTTTGTCCATAAACCCTGACA
AATCTCCCATGCCTAATAACAACTGACAAATGGTNTTAACATCAAAAACT
TCAAATTCATACCAT

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