AM162121

EST Overview
NameAM162121
Unique NameAM162121
TypeEST
OrganismPisum sativum (pea)
Sequence length439
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v2Pisum sativum unigene v2EST
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of AM162121 vs. TrEMBL
Match: C6T8P2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.402e-9
Identity = 39/80 (48.75%), Postives = 48/80 (60.00%), Query Frame = -2 
Query:  199 SLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIW-RRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVLG 435
            SLAKTVF SDYRDIL+KVETPCTIIQT       +  N  ++ + K+KGK+                  +LVDV+KGVLG
Sbjct:  201 SLAKTVFYSDYRDILDKVETPCTIIQT--SSDIVVPHNAAVYMKNKIKGKVTLEFVDTKGHFPQLTARLQLVDVIKGVLG 278
[Close]
BLAST of AM162121 vs. TrEMBL
Match: C6TKX7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.688e-8
Identity = 40/83 (48.19%), Postives = 46/83 (55.42%), Query Frame = -2 
Query:  199 SLAKTVFCSDYRDILEKVETPCTIIQT----F*RHGCSI*CNTLIWRRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVLG 435
            SLAKTVF SDYRDILEKVETPCTIIQT       H  ++         K+KGK+                  +LVDV+KGVLG
Sbjct:  201 SLAKTVFYSDYRDILEKVETPCTIIQTSSDIVVPHKAAV-----YMESKIKGKVTLEVVDTKGHFPQLTASLQLVDVIKGVLG 278
[Close]
BLAST of AM162121 vs. TrEMBL
Match: A9PH43_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 40.817 bits (94), Expect = 3.463e-8
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = -1 
Query:  230 MEKKIKGKVTLEVIDTFGHFPQLTAPLQ 313
            M++KIKGK T+E+I+T GHFP LTA  Q
Sbjct:  232 MQEKIKGKSTVEIIETDGHFPHLTAHQQ 259

HSP 2 Score: 40.4318 bits (93), Expect = 3.463e-8
Identity = 19/26 (73.08%), Postives = 21/26 (80.77%), Query Frame = -2 
Query:  355 LAKTVFCSDYRDILEKVETPCTIIQT 432
            +AKTVF  D RDILEKV TPC I+QT
Sbjct:  193 VAKTVFYCDERDILEKVLTPCIIVQT 218
[Close]
BLAST of AM162121 vs. TrEMBL
Match: B9H996_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_416208 PE=4 SV=1)

HSP 1 Score: 40.817 bits (94), Expect = 3.471e-8
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = -1 
Query:  230 MEKKIKGKVTLEVIDTFGHFPQLTAPLQ 313
            M++KIKGK T+E+I+T GHFP LTA  Q
Sbjct:  232 MQEKIKGKSTVEIIETDGHFPHLTAHQQ 259

HSP 2 Score: 40.4318 bits (93), Expect = 3.471e-8
Identity = 19/26 (73.08%), Postives = 21/26 (80.77%), Query Frame = -2 
Query:  355 LAKTVFCSDYRDILEKVETPCTIIQT 432
            +AKTVF  D RDILEKV TPC I+QT
Sbjct:  193 VAKTVFYCDERDILEKVLTPCIIVQT 218
[Close]
BLAST of AM162121 vs. TrEMBL
Match: C6TLG3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 8.379e-8
Identity = 35/80 (43.75%), Postives = 47/80 (58.75%), Query Frame = -2 
Query:  202 LSLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIW-RRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVL 438
            LSLAKT+F SD+R+IL+KVETPCTIIQT  +   ++  N  ++ + K+KGK+                H K V VLK  L
Sbjct:  192 LSLAKTIFYSDWREILDKVETPCTIIQT--KKDAAVPHNVALYMKNKIKGKVTLEIIDTLGHFPQLTAHLKFVQVLKAAL 269
[Close]
BLAST of AM162121 vs. TAIR peptide
Match: AT3G24420.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:8863111-8864883 REVERSE LENGTH=273)

HSP 1 Score: 38.1206 bits (87), Expect = 1.169e-7
Identity = 17/29 (58.62%), Postives = 24/29 (82.76%), Query Frame = -1 
Query:  230 MEKKIKGKVTLEVI-DTFGHFPQLTAPLQ 313
            M++KIKGK T+E+I D  GHFPQ+T+ L+
Sbjct:  234 MQEKIKGKSTVEIIEDAIGHFPQMTSHLE 262

HSP 2 Score: 33.8834 bits (76), Expect = 1.169e-7
Identity = 16/27 (59.26%), Postives = 20/27 (74.07%), Query Frame = -2 
Query:  358 LSLAKTVFCSDYRDILEKVETPCTIIQ 438
            L+LAK VF SD R+IL +V  PC +IQ
Sbjct:  193 LALAKIVFGSDEREILGQVSVPCHVIQ 219
[Close]
BLAST of AM162121 vs. TAIR peptide
Match: AT3G24420.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:8863111-8864883 REVERSE LENGTH=273)

HSP 1 Score: 38.1206 bits (87), Expect = 1.169e-7
Identity = 17/29 (58.62%), Postives = 24/29 (82.76%), Query Frame = -1 
Query:  230 MEKKIKGKVTLEVI-DTFGHFPQLTAPLQ 313
            M++KIKGK T+E+I D  GHFPQ+T+ L+
Sbjct:  234 MQEKIKGKSTVEIIEDAIGHFPQMTSHLE 262

HSP 2 Score: 33.8834 bits (76), Expect = 1.169e-7
Identity = 16/27 (59.26%), Postives = 20/27 (74.07%), Query Frame = -2 
Query:  358 LSLAKTVFCSDYRDILEKVETPCTIIQ 438
            L+LAK VF SD R+IL +V  PC +IQ
Sbjct:  193 LALAKIVFGSDEREILGQVSVPCHVIQ 219
[Close]
BLAST of AM162121 vs. TrEMBL
Match: C6T8P2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.254e-9
Identity = 39/80 (48.75%), Postives = 48/80 (60.00%), Query Frame = -2 
Query:  199 SLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIW-RRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVLG 435
            SLAKTVF SDYRDIL+KVETPCTIIQT       +  N  ++ + K+KGK+                  +LVDV+KGVLG
Sbjct:  201 SLAKTVFYSDYRDILDKVETPCTIIQT--SSDIVVPHNAAVYMKNKIKGKVTLEFVDTKGHFPQLTARLQLVDVIKGVLG 278
[Close]
BLAST of AM162121 vs. TrEMBL
Match: C6TKX7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 2.111e-8
Identity = 40/83 (48.19%), Postives = 46/83 (55.42%), Query Frame = -2 
Query:  199 SLAKTVFCSDYRDILEKVETPCTIIQT----F*RHGCSI*CNTLIWRRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVLG 435
            SLAKTVF SDYRDILEKVETPCTIIQT       H  ++         K+KGK+                  +LVDV+KGVLG
Sbjct:  201 SLAKTVFYSDYRDILEKVETPCTIIQTSSDIVVPHKAAV-----YMESKIKGKVTLEVVDTKGHFPQLTASLQLVDVIKGVLG 278
[Close]
BLAST of AM162121 vs. TrEMBL
Match: A9PH43_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 40.817 bits (94), Expect = 4.331e-8
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = -1 
Query:  230 MEKKIKGKVTLEVIDTFGHFPQLTAPLQ 313
            M++KIKGK T+E+I+T GHFP LTA  Q
Sbjct:  232 MQEKIKGKSTVEIIETDGHFPHLTAHQQ 259

HSP 2 Score: 40.4318 bits (93), Expect = 4.331e-8
Identity = 19/26 (73.08%), Postives = 21/26 (80.77%), Query Frame = -2 
Query:  355 LAKTVFCSDYRDILEKVETPCTIIQT 432
            +AKTVF  D RDILEKV TPC I+QT
Sbjct:  193 VAKTVFYCDERDILEKVLTPCIIVQT 218
[Close]
BLAST of AM162121 vs. TrEMBL
Match: B9H996_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_416208 PE=4 SV=1)

HSP 1 Score: 40.817 bits (94), Expect = 4.340e-8
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = -1 
Query:  230 MEKKIKGKVTLEVIDTFGHFPQLTAPLQ 313
            M++KIKGK T+E+I+T GHFP LTA  Q
Sbjct:  232 MQEKIKGKSTVEIIETDGHFPHLTAHQQ 259

HSP 2 Score: 40.4318 bits (93), Expect = 4.340e-8
Identity = 19/26 (73.08%), Postives = 21/26 (80.77%), Query Frame = -2 
Query:  355 LAKTVFCSDYRDILEKVETPCTIIQT 432
            +AKTVF  D RDILEKV TPC I+QT
Sbjct:  193 VAKTVFYCDERDILEKVLTPCIIVQT 218
[Close]
BLAST of AM162121 vs. TrEMBL
Match: C6TLG3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.048e-7
Identity = 35/80 (43.75%), Postives = 47/80 (58.75%), Query Frame = -2 
Query:  202 LSLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIW-RRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVL 438
            LSLAKT+F SD+R+IL+KVETPCTIIQT  +   ++  N  ++ + K+KGK+                H K V VLK  L
Sbjct:  192 LSLAKTIFYSDWREILDKVETPCTIIQT--KKDAAVPHNVALYMKNKIKGKVTLEIIDTLGHFPQLTAHLKFVQVLKAAL 269
[Close]
BLAST of AM162121 vs. Lotus protein
Match: chr2.CM0153.40.r2.d (- phase: 0 /partial)

HSP 1 Score: 67.781 bits (164), Expect = 1.675e-12
Identity = 38/85 (44.71%), Postives = 53/85 (62.35%), Query Frame = -2 
Query:  187 LSLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIW-RRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVLGFELS 438
            +SLAKTVFC DYRDILEKVET CTIIQ+   +   +  +  ++  +K+KGK+                H +L+D++KGVLGF L+
Sbjct:    7 VSLAKTVFCCDYRDILEKVETACTIIQS--SNDMVVPYSVALYMEKKIKGKVTLEVIDTVGHFPQLTAHLQLIDLIKGVLGFGLT 89
[Close]
BLAST of AM162121 vs. Soybean peptides
Match: Glyma09g31970.1|PACid:16276886 ()

HSP 1 Score: 63.929 bits (154), Expect = 5.747e-11
Identity = 39/80 (48.75%), Postives = 48/80 (60.00%), Query Frame = -2 
Query:  199 SLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIW-RRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVLG 435
            SLAKTVF SDYRDIL+KVETPCTIIQT       +  N  ++ + K+KGK+                  +LVDV+KGVLG
Sbjct:  201 SLAKTVFYSDYRDILDKVETPCTIIQT--SSDIVVPHNAAVYMKNKIKGKVTLEFVDTKGHFPQLTAWLQLVDVIKGVLG 278
[Close]
BLAST of AM162121 vs. Soybean peptides
Match: Glyma07g09840.1|PACid:16266797 ()

HSP 1 Score: 62.3882 bits (150), Expect = 1.672e-10
Identity = 40/83 (48.19%), Postives = 46/83 (55.42%), Query Frame = -2 
Query:  199 SLAKTVFCSDYRDILEKVETPCTIIQT----F*RHGCSI*CNTLIWRRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVLG 435
            SLAKTVF SDYRDILEKVETPCTIIQT       H  ++         K+KGK+                  +LVDV+KGVLG
Sbjct:  201 SLAKTVFYSDYRDILEKVETPCTIIQTSSDIVVPHKAAV-----YMESKIKGKVTLEVVDTKGHFPQLTASLQLVDVIKGVLG 278
[Close]
BLAST of AM162121 vs. Soybean peptides
Match: Glyma05g35270.1|PACid:16260772 ()

HSP 1 Score: 62.3882 bits (150), Expect = 1.672e-10
Identity = 36/80 (45.00%), Postives = 48/80 (60.00%), Query Frame = -2 
Query:  202 LSLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIW-RRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVL 438
            LSLAKT+F SD+R+IL+KVETPCTIIQT  +   ++  N  ++   K+KGK+                H K V+VLKG L
Sbjct:  166 LSLAKTIFYSDWREILDKVETPCTIIQT--KKDAAVPHNVALYMENKIKGKVTLEIIDTLGHFPQLTAHLKFVEVLKGAL 243
[Close]
BLAST of AM162121 vs. Soybean peptides
Match: Glyma08g04450.1|PACid:16269937 ()

HSP 1 Score: 60.077 bits (144), Expect = 8.298e-10
Identity = 35/80 (43.75%), Postives = 47/80 (58.75%), Query Frame = -2 
Query:  202 LSLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIW-RRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVL 438
            LSLAKT+F SD+R+IL+KVETPCTIIQT  +   ++  N  ++ + K+KGK+                H K V VLK  L
Sbjct:  192 LSLAKTIFYSDWREILDKVETPCTIIQT--KKDAAVPHNVALYMKNKIKGKVTLEIIDTLGHFPQLTAHLKFVQVLKAAL 269
[Close]
BLAST of AM162121 vs. Soybean peptides
Match: Glyma20g08560.1|PACid:16315734 ()

HSP 1 Score: 56.225 bits (134), Expect = 1.198e-8
Identity = 35/79 (44.30%), Postives = 43/79 (54.43%), Query Frame = -2 
Query:  202 LSLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIWRRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVL 438
            +SLAKTVF SDYRD+LEKV+ PCTIIQ+       +     +   K+KG                  H KLV+VLKGVL
Sbjct:  191 ISLAKTVFYSDYRDMLEKVQIPCTIIQSSNDMAVPLNIGHYL-EEKIKGVSTLEIIDMIGHFPHLTAHLKLVEVLKGVL 268
[Close]
BLAST of AM162121 vs. Medicago proteins
Match: IMGA|Medtr6g086560.1 (Sigma factor sigB regulation protein rsbQ (AHRD V1 ***- O07015); contains Interpro domain(s) IPR000073 Alpha/beta hydrolase fold-1 chr06_pseudomolecule_IMGAG_V3.5 20278664-20274301 E EGN_Mt100125 20100825)

HSP 1 Score: 70.8626 bits (172), Expect = 2.831e-13
Identity = 43/82 (52.44%), Postives = 48/82 (58.54%), Query Frame = -2 
Query:  193 LSLAKTVFCSDYRDILEKVETPCTIIQTF*RHGCSI*CNTLIWRRKLKGKLHWRXXXXXXXXXXXXLHFKLVDVLKGVLGFE 438
            LSLAKTVFC DYRDILEKVETPCTIIQT            L   +K+KGK+                  +LVDVLKGVLGF+
Sbjct:  201 LSLAKTVFCHDYRDILEKVETPCTIIQTSSDMVVPY-SVALYMEKKIKGKVTLEVIDTFGHFPQLTAPLQLVDVLKGVLGFD 281
The following BLAST results are available for this feature:
BLAST of AM162121 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 5
Match NameE-valueIdentityDescription
C6T8P2_SOYBN3.402e-948.75Putative uncharacterized protein OS=Glycine max PE... [more]
C6TKX7_SOYBN1.688e-848.19Putative uncharacterized protein OS=Glycine max PE... [more]
A9PH43_POPTR3.463e-864.29Putative uncharacterized protein OS=Populus tricho... [more]
B9H996_POPTR3.471e-864.29Predicted protein (Fragment) OS=Populus trichocarp... [more]
C6TLG3_SOYBN8.379e-843.75Putative uncharacterized protein OS=Glycine max PE... [more]
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BLAST of AM162121 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT3G24420.11.169e-758.62| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
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BLAST of AM162121 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT3G24420.11.169e-758.62| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
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BLAST of AM162121 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 5
Match NameE-valueIdentityDescription
C6T8P2_SOYBN4.254e-948.75Putative uncharacterized protein OS=Glycine max PE... [more]
C6TKX7_SOYBN2.111e-848.19Putative uncharacterized protein OS=Glycine max PE... [more]
A9PH43_POPTR4.331e-864.29Putative uncharacterized protein OS=Populus tricho... [more]
B9H996_POPTR4.340e-864.29Predicted protein (Fragment) OS=Populus trichocarp... [more]
C6TLG3_SOYBN1.048e-743.75Putative uncharacterized protein OS=Glycine max PE... [more]
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BLAST of AM162121 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 1
Match NameE-valueIdentityDescription
chr2.CM0153.40.r2.d1.675e-1244.71- phase: 0 /partial[more]
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BLAST of AM162121 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 5
Match NameE-valueIdentityDescription
Glyma09g31970.1|PACid:162768865.747e-1148.75[more]
Glyma07g09840.1|PACid:162667971.672e-1048.19[more]
Glyma05g35270.1|PACid:162607721.672e-1045.00[more]
Glyma08g04450.1|PACid:162699378.298e-1043.75[more]
Glyma20g08560.1|PACid:163157341.198e-844.30[more]
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BLAST of AM162121 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 1
Match NameE-valueIdentityDescription
IMGA|Medtr6g086560.12.831e-1352.44Sigma factor sigB regulation protein rsbQ (AHRD V1... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v2
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM162121 ID=AM162121; Name=AM162121; organism=Pisum sativum; type=EST; length=439bp
AATTAACACCACTAATCAATATTAATAACTAATTCAAAAATACACACAAT
AAATTATTAATAAATCCCAAACATACTATTTCCACAACATTACTAAAGAA
AACCATCAATTTTACAAACAAGTAAAATCAAATGTTACAAAAGAAATGAA
ATTGAAAATAACCCTACACATACAATACTAGACATGACTAAGCTCAAACC
CTAAAACACCCTTAAGAACATCAACTAGTTTGAAGTGGAGCAGTTAATTG
AGGAAAATGTCCAAAAGTGTCAATTACCTCCAATGTAACTTTCCCTTTAA
TTTTCTTCTCCATATCAAAGTGTTACACTATATGGAACAGCCATGTCGCT
AGAAGGTTTGAATTATTGTGCATGGAGTTTCAACTTTCTCAAGTATATCT
CTATAGTCACTGCAAAACACTGTCTTGGCTAAGGACAAA
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