GH720135

EST Overview


Name	GH720135
Unique Name	GH720135
Type	EST
Organism	Pisum sativum (pea)
Sequence length	355

Libraries


Library Name	Type
LIBEST_014004 TNE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of GH720135 vs. TrEMBL
Match: Q0IJ86_SOYBN (Chloride channel OS=Glycine max PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.485e-44
Identity = 93/117 (79.49%), Postives = 98/117 (83.76%), Query Frame = -3

Query:    3 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDDGV 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAKTVGD FNPSIYEIILHLKGLPFMDANP  WMRNLTVGELVDVKPAV+S +GVEKVA IV+ LKNTTHNGFPVMD G+
Sbjct:  519 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKTVGDSFNPSIYEIILHLKGLPFMDANPEPWMRNLTVGELVDVKPAVVSFKGVEKVANIVNALKNTTHNGFPVMDCGL 635

[Close]

BLAST of GH720135 vs. TrEMBL
Match: D7SVR2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_68.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033108001 PE=4 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.233e-43
Identity = 88/117 (75.21%), Postives = 99/117 (84.62%), Query Frame = -3

Query:    3 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDDGV 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAK+VGDC NPSIY+IILHLKGLPF+DANP  WMRNLTVGEL D KP V++L+GVEKVA+IVDVL+NTTHNGFPV+D+GV
Sbjct:  521 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKSVGDCINPSIYDIILHLKGLPFLDANPEPWMRNLTVGELADAKPPVVTLRGVEKVARIVDVLRNTTHNGFPVVDEGV 637

[Close]

BLAST of GH720135 vs. TrEMBL
Match: B9RBJ7_RICCO (Chloride channel clc, putative OS=Ricinus communis GN=RCOM_1677610 PE=4 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 6.121e-43
Identity = 89/117 (76.07%), Postives = 98/117 (83.76%), Query Frame = -3

Query:    3 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDDGV 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAKTVGD FNPSIYEIILHLKGLPF+DANP  WMRNLTVGEL D KP +++L GVEKV++IVDVLKNTT+NGFPV+DDGV
Sbjct:  519 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKTVGDSFNPSIYEIILHLKGLPFLDANPEPWMRNLTVGELADAKPPLVTLCGVEKVSRIVDVLKNTTYNGFPVVDDGV 635

[Close]

BLAST of GH720135 vs. TrEMBL
Match: D7LLW8_ARALY (CLC-B OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_484500 PE=4 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 4.387e-41
Identity = 85/115 (73.91%), Postives = 96/115 (83.48%), Query Frame = -3

Query:    9 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDD 353
            AA+LMAGSMRMTVSLCVIFLELT+N            IAKTVGD FNPSIY+IILHLKGLPF++ANP  WMRNL+VGEL D KP V++LQGVEKV+KIVDVLKNTTHN FPV+D+
Sbjct:  512 AAALMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPEPWMRNLSVGELGDAKPPVVTLQGVEKVSKIVDVLKNTTHNAFPVLDE 626

[Close]

BLAST of GH720135 vs. TrEMBL
Match: B9GJS4_POPTR (Cl-channel clc-7 OS=Populus trichocarpa GN=POPTRDRAFT_829777 PE=4 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 1.667e-40
Identity = 85/117 (72.65%), Postives = 96/117 (82.05%), Query Frame = -3

Query:    3 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDDGV 353
            AASLMAGSMRMTVSLCVIFLELT+N            I+KTVGD FNPSIYEIIL LKGLPF+DANP  WMRNLTV EL D KP V++L GVEKV++IV+VL+NTTHNGFPV+D+GV
Sbjct:  517 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLISKTVGDSFNPSIYEIILDLKGLPFLDANPEPWMRNLTVAELADAKPPVVTLCGVEKVSRIVEVLENTTHNGFPVVDEGV 633

[Close]

BLAST of GH720135 vs. TrEMBL
Match: Q9XF71_TOBAC (CLC-Nt2 protein OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 3.714e-40
Identity = 84/117 (71.79%), Postives = 95/117 (81.20%), Query Frame = -3

Query:    3 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDDGV 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAK+VGDCFN SIYEIIL LKGLPF+DANP  WMRN+T GEL DVKP V++L GVEKV +IV+ LKNTT+NGFPV+D+GV
Sbjct:  518 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMLVLLIAKSVGDCFNLSIYEIILELKGLPFLDANPEPWMRNITAGELADVKPPVVTLCGVEKVGRIVEALKNTTYNGFPVVDEGV 634

[Close]

BLAST of GH720135 vs. TrEMBL
Match: D3YP02_THEHA (Chloride channel A OS=Thellungiella halophila GN=CLC-A PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 3.714e-40
Identity = 87/114 (76.32%), Postives = 91/114 (79.82%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAKTVGD FN SIYEIILHLKGLPF+DANP  WMRNLTVGEL D KP VISL GVEKVA IVD L+NTTHN FPV+D
Sbjct:  513 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLDANPEPWMRNLTVGELGDAKPPVISLHGVEKVANIVDALRNTTHNAFPVLD 626

[Close]

BLAST of GH720135 vs. TrEMBL
Match: Q96325_ARATH (Voltage-gated chloride channel OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.080e-39
Identity = 85/114 (74.56%), Postives = 92/114 (80.70%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAKTVGD FN SIYEIILHLKGLPF++ANP  WMRNLTVGEL D KP V++L GVEKVA IVDVL+NTTHN FPV+D
Sbjct:  512 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLRNTTHNAFPVLD 625

[Close]

BLAST of GH720135 vs. TrEMBL
Match: Q2V324_ARATH (Putative uncharacterized protein At5g40890.2 OS=Arabidopsis thaliana GN=At5g40890 PE=4 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.080e-39
Identity = 85/114 (74.56%), Postives = 92/114 (80.70%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAKTVGD FN SIYEIILHLKGLPF++ANP  WMRNLTVGEL D KP V++L GVEKVA IVDVL+NTTHN FPV+D
Sbjct:  382 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLRNTTHNAFPVLD 495

[Close]

BLAST of GH720135 vs. TrEMBL
Match: D7MJ14_ARALY (ATCLC-A OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_493886 PE=4 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.080e-39
Identity = 85/114 (74.56%), Postives = 92/114 (80.70%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAKTVGD FN SIYEIILHLKGLPF++ANP  WMRNLTVGEL D KP V++L GVEKVA IVDVL+NTTHN FPV+D
Sbjct:  515 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLRNTTHNAFPVLD 628

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCB_ARATH (Chloride channel protein CLC-b OS=Arabidopsis thaliana GN=CLC-B PE=1 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 2.889e-42
Identity = 85/115 (73.91%), Postives = 95/115 (82.61%), Query Frame = -3

Query:    9 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDD 353
            AA+LMAGSMRMTVSLCVIFLELT+N            IAKTVGD FNPSIY+IILHLKGLPF++ANP  WMRNLTVGEL D KP V++LQGVEKV+ IVDVLKNTTHN FPV+D+
Sbjct:  513 AAALMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPPVVTLQGVEKVSNIVDVLKNTTHNAFPVLDE 627

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCA_ARATH (Chloride channel protein CLC-a OS=Arabidopsis thaliana GN=CLC-A PE=1 SV=2)

HSP 1 Score: 166.007 bits (419), Expect = 5.448e-41
Identity = 85/114 (74.56%), Postives = 92/114 (80.70%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAKTVGD FN SIYEIILHLKGLPF++ANP  WMRNLTVGEL D KP V++L GVEKVA IVDVL+NTTHN FPV+D
Sbjct:  514 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLRNTTHNAFPVLD 627

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCC_ARATH (Chloride channel protein CLC-c OS=Arabidopsis thaliana GN=CLC-C PE=1 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 1.141e-22
Identity = 57/115 (49.57%), Postives = 74/115 (64.35%), Query Frame = -3

Query:    9 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDD 353
            AAS + G+MRMTVSLCVI LELT+N            I+KTV DCFN  +Y+ I+ +KGLP+M+ +   +MRNL   ++  V  A+IS   VEKV  I   LK T HNGFPV+D+
Sbjct:  524 AASFLGGTMRMTVSLCVILLELTNNLLMLPLVMLVLLISKTVADCFNRGVYDQIVTMKGLPYMEDHAEPYMRNLVAKDV--VSGALISFSRVEKVGVIWQALKMTRHNGFPVIDE 636

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCG_ARATH (Putative chloride channel-like protein CLC-g OS=Arabidopsis thaliana GN=At5g33280 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 9.662e-22
Identity = 56/114 (49.12%), Postives = 73/114 (64.04%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AAS + G+MRMTVS CVI LELT+N            I+KTV D FN +IY +I+ LKG P++ ++   +MR L VG++V     V +  G+EKV  IV VLK T HNGFPV+D
Sbjct:  496 AASFLGGTMRMTVSTCVILLELTNNLLLLPMMMVVLLISKTVADGFNANIYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQVFN--GIEKVETIVHVLKTTNHNGFPVVD 607

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCD_ARATH (Chloride channel protein CLC-d OS=Arabidopsis thaliana GN=CLC-D PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 4.488e-19
Identity = 50/114 (43.86%), Postives = 67/114 (58.77%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AAS + GSMRMTVSLCVI +E+T+N            I+K VGD FN  +YE+   LKG+P +++ P   MR +   E       VISL  V +VA +  +L +  HNGFPV+D
Sbjct:  512 AASFLGGSMRMTVSLCVIMVEITNNLKLLPLIMLVLLISKAVGDAFNEGLYEVQARLKGIPLLESRPKYHMRQMIAKEACQ-SQKVISLPRVIRVADVASILGSNKHNGFPVID 624

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCA_DICDI (Chloride channel protein A OS=Dictyostelium discoideum GN=clcA PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 4.644e-16
Identity = 48/116 (41.38%), Postives = 71/116 (61.21%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTT--HNGFPVMD 353
            A+++MAG  RMT+SL +I +ELT              IAK VGD FN SIYE ++  K  PF+ + P   M  L V ++  +K  V++L  VE+V+K+++VLK+    HNGFPV++
Sbjct:  582 ASAMMAGFSRMTISLAIIMVELTEGTQYMLPVILSVMIAKWVGDFFNESIYEHLIEQKCYPFLQSQPPKSMIKLGVVDI--MKTEVVTLHEVERVSKVIEVLKSEQHFHNGFPVIE 695

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCB_DICDI (Chloride channel protein B OS=Dictyostelium discoideum GN=clcB PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 7.922e-16
Identity = 45/113 (39.82%), Postives = 66/113 (58.41%), Query Frame = -3

Query:   15 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVM 353
            AAS+MAGS+RMT+SL VI +ELT              + K  GD FN SIYE ++ LK +P++++ P + +R  TV E +     V +L  + KV   + +L+   HNGFPV+
Sbjct:  528 AASMMAGSLRMTISLVVIIVELTETTQYLLPVILSVMVGKWCGDIFNESIYEHLIELKHIPYLNSQPPNHLRKKTVAEAMSTD--VKTLPEIVKVKTALTILETCPHNGFPVV 638

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCC_DICDI (Chloride channel protein C OS=Dictyostelium discoideum GN=clcC PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.157e-13
Identity = 40/116 (34.48%), Postives = 65/116 (56.03%), Query Frame = -3

Query:    6 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDDG 353
            +A++MAG  RMTV + VI +ELT              I+K VGD FN S+YE ++  K +PF+ + P     N+ + +++     V+ L  V +V  +V++L +  HN FPV++ G
Sbjct:  521 SAAMMAGYCRMTVCIVVIMVELTEGTQYLVPIILAVMISKWVGDFFNESVYEHLMEQKSIPFLQSKPPHSTNNIRISDVMSKN--VVVLPEVCQVRLLVNILNSNNHNAFPVINSG 634

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCN7_RAT (H(+)/Cl(-) exchange transporter 7 OS=Rattus norvegicus GN=Clcn7 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.398e-12
Identity = 45/117 (38.46%), Postives = 64/117 (54.70%), Query Frame = -3

Query:    9 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNT--THNGFPVMDD 353
            AA+ + G +RMT+SL VI +E TSN             AK VGD F   +Y++ + L+ +PF+         +LT  E++     V  L+  EKV  IVDVL +T   HNGFPV++D
Sbjct:  550 AAAQLGGIVRMTLSLTVIMMEATSNVTYGFPIMLVLMTAKIVGDVFIEGLYDMHIQLQSVPFLHWEAPVTSHSLTAREVMST--PVTCLRRREKVGIIVDVLSDTASNHNGFPVVED 664

[Close]

BLAST of GH720135 vs. SwissProt
Match: CLCN7_MOUSE (H(+)/Cl(-) exchange transporter 7 OS=Mus musculus GN=Clcn7 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.398e-12
Identity = 45/117 (38.46%), Postives = 64/117 (54.70%), Query Frame = -3

Query:    9 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNT--THNGFPVMDD 353
            AA+ + G +RMT+SL VI +E TSN             AK VGD F   +Y++ + L+ +PF+         +LT  E++     V  L+  EKV  IVDVL +T   HNGFPV++D
Sbjct:  550 AAAQLGGIVRMTLSLTVIMMEATSNVTYGFPIMLVLMTAKIVGDVFIEGLYDMHIQLQSVPFLHWEAPVTSHSLTAREVMST--PVTCLRRREKVGIIVDVLSDTASNHNGFPVVED 664

[Close]

BLAST of GH720135 vs. TAIR peptide
Match: AT3G27170.1 (| Symbols: CLC-B, ATCLC-B | chloride channel B | chr3:10024147-10026921 FORWARD LENGTH=780)

HSP 1 Score: 170.244 bits (430), Expect = 2.223e-43
Identity = 85/115 (73.91%), Postives = 95/115 (82.61%), Query Frame = -3

Query:    9 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDD 353
            AA+LMAGSMRMTVSLCVIFLELT+N            IAKTVGD FNPSIY+IILHLKGLPF++ANP  WMRNLTVGEL D KP V++LQGVEKV+ IVDVLKNTTHN FPV+D+
Sbjct:  513 AAALMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPPVVTLQGVEKVSNIVDVLKNTTHNAFPVLDE 627

[Close]

BLAST of GH720135 vs. TAIR peptide
Match: AT5G40890.2 (| Symbols: ATCLC-A, CLC-A, CLCA, ATCLCA | chloride channel A | chr5:16381645-16383821 REVERSE LENGTH=643)

HSP 1 Score: 166.007 bits (419), Expect = 4.193e-42
Identity = 85/114 (74.56%), Postives = 92/114 (80.70%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAKTVGD FN SIYEIILHLKGLPF++ANP  WMRNLTVGEL D KP V++L GVEKVA IVDVL+NTTHN FPV+D
Sbjct:  382 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLRNTTHNAFPVLD 495

[Close]

BLAST of GH720135 vs. TAIR peptide
Match: AT5G40890.1 (| Symbols: ATCLC-A, CLC-A, CLCA, ATCLCA | chloride channel A | chr5:16381645-16384999 REVERSE LENGTH=775)

HSP 1 Score: 166.007 bits (419), Expect = 4.193e-42
Identity = 85/114 (74.56%), Postives = 92/114 (80.70%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AASLMAGSMRMTVSLCVIFLELT+N            IAKTVGD FN SIYEIILHLKGLPF++ANP  WMRNLTVGEL D KP V++L GVEKVA IVDVL+NTTHN FPV+D
Sbjct:  514 AASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLRNTTHNAFPVLD 627

[Close]

BLAST of GH720135 vs. TAIR peptide
Match: AT5G49890.1 (| Symbols: CLC-C, ATCLC-C | chloride channel C | chr5:20288489-20292143 REVERSE LENGTH=779)

HSP 1 Score: 105.145 bits (261), Expect = 8.783e-24
Identity = 57/115 (49.57%), Postives = 74/115 (64.35%), Query Frame = -3

Query:    9 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMDD 353
            AAS + G+MRMTVSLCVI LELT+N            I+KTV DCFN  +Y+ I+ +KGLP+M+ +   +MRNL   ++  V  A+IS   VEKV  I   LK T HNGFPV+D+
Sbjct:  524 AASFLGGTMRMTVSLCVILLELTNNLLMLPLVMLVLLISKTVADCFNRGVYDQIVTMKGLPYMEDHAEPYMRNLVAKDV--VSGALISFSRVEKVGVIWQALKMTRHNGFPVIDE 636

[Close]

BLAST of GH720135 vs. TAIR peptide
Match: AT5G33280.1 (| Symbols: | Voltage-gated chloride channel family protein | chr5:12549280-12552305 FORWARD LENGTH=765)

HSP 1 Score: 102.064 bits (253), Expect = 7.435e-23
Identity = 56/114 (49.12%), Postives = 73/114 (64.04%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AAS + G+MRMTVS CVI LELT+N            I+KTV D FN +IY +I+ LKG P++ ++   +MR L VG++V     V +  G+EKV  IV VLK T HNGFPV+D
Sbjct:  498 AASFLGGTMRMTVSTCVILLELTNNLLLLPMMMVVLLISKTVADGFNANIYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQVFN--GIEKVETIVHVLKTTNHNGFPVVD 609

[Close]

BLAST of GH720135 vs. TAIR peptide
Match: AT5G26240.1 (| Symbols: CLC-D, ATCLC-D | chloride channel D | chr5:9189622-9194347 FORWARD LENGTH=792)

HSP 1 Score: 93.2041 bits (230), Expect = 3.454e-20
Identity = 50/114 (43.86%), Postives = 67/114 (58.77%), Query Frame = -3

Query:   12 AASLMAGSMRMTVSLCVIFLELTSNXXXXXXXXXXXXIAKTVGDCFNPSIYEIILHLKGLPFMDANPASWMRNLTVGELVDVKPAVISLQGVEKVAKIVDVLKNTTHNGFPVMD 353
            AAS + GSMRMTVSLCVI +E+T+N            I+K VGD FN  +YE+   LKG+P +++ P   MR +   E       VISL  V +VA +  +L +  HNGFPV+D
Sbjct:  512 AASFLGGSMRMTVSLCVIMVEITNNLKLLPLIMLVLLISKAVGDAFNEGLYEVQARLKGIPLLESRPKYHMRQMIAKEACQ-SQKVISLPRVIRVADVASILGSNKHNGFPVID 624

The following BLAST results are available for this feature:

BLAST of GH720135 vs. TrEMBL
BLAST of GH720135 vs. SwissProt
BLAST of GH720135 vs. TAIR peptide

BLAST of GH720135 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q0IJ86_SOYBN	2.485e-44	79.49	Chloride channel OS=Glycine max PE=2 SV=1	[more]
D7SVR2_VITVI	1.233e-43	75.21	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RBJ7_RICCO	6.121e-43	76.07	Chloride channel clc, putative OS=Ricinus communis...	[more]
D7LLW8_ARALY	4.387e-41	73.91	CLC-B OS=Arabidopsis lyrata subsp. lyrata GN=ARALY...	[more]
B9GJS4_POPTR	1.667e-40	72.65	Cl-channel clc-7 OS=Populus trichocarpa GN=POPTRDR...	[more]
Q9XF71_TOBAC	3.714e-40	71.79	CLC-Nt2 protein OS=Nicotiana tabacum PE=2 SV=1	[more]
D3YP02_THEHA	3.714e-40	76.32	Chloride channel A OS=Thellungiella halophila GN=C...	[more]
Q96325_ARATH	1.080e-39	74.56	Voltage-gated chloride channel OS=Arabidopsis thal...	[more]
Q2V324_ARATH	1.080e-39	74.56	Putative uncharacterized protein At5g40890.2 OS=Ar...	[more]
D7MJ14_ARALY	1.080e-39	74.56	ATCLC-A OS=Arabidopsis lyrata subsp. lyrata GN=ARA...	[more]

BLAST of GH720135 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CLCB_ARATH	2.889e-42	73.91	Chloride channel protein CLC-b OS=Arabidopsis thal...	[more]
CLCA_ARATH	5.448e-41	74.56	Chloride channel protein CLC-a OS=Arabidopsis thal...	[more]
CLCC_ARATH	1.141e-22	49.57	Chloride channel protein CLC-c OS=Arabidopsis thal...	[more]
CLCG_ARATH	9.662e-22	49.12	Putative chloride channel-like protein CLC-g OS=Ar...	[more]
CLCD_ARATH	4.488e-19	43.86	Chloride channel protein CLC-d OS=Arabidopsis thal...	[more]
CLCA_DICDI	4.644e-16	41.38	Chloride channel protein A OS=Dictyostelium discoi...	[more]
CLCB_DICDI	7.922e-16	39.82	Chloride channel protein B OS=Dictyostelium discoi...	[more]
CLCC_DICDI	2.157e-13	34.48	Chloride channel protein C OS=Dictyostelium discoi...	[more]
CLCN7_RAT	1.398e-12	38.46	H(+)/Cl(-) exchange transporter 7 OS=Rattus norveg...	[more]
CLCN7_MOUSE	1.398e-12	38.46	H(+)/Cl(-) exchange transporter 7 OS=Mus musculus ...	[more]

BLAST of GH720135 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT3G27170.1	2.223e-43	73.91	\| Symbols: CLC-B, ATCLC-B \| chloride channel B \| c...	[more]
AT5G40890.2	4.193e-42	74.56	\| Symbols: ATCLC-A, CLC-A, CLCA, ATCLCA \| chloride...	[more]
AT5G40890.1	4.193e-42	74.56	\| Symbols: ATCLC-A, CLC-A, CLCA, ATCLCA \| chloride...	[more]
AT5G49890.1	8.783e-24	49.57	\| Symbols: CLC-C, ATCLC-C \| chloride channel C \| c...	[more]
AT5G33280.1	7.435e-23	49.12	\| Symbols: \| Voltage-gated chloride channel famil...	[more]
AT5G26240.1	3.454e-20	43.86	\| Symbols: CLC-D, ATCLC-D \| chloride channel D \| c...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001807	Chloride channel, voltage gated	PRINTS	PR00762	CLCHANNEL	coord: 12..31 score: 8.3e-09coord: 50..64 score: 8.3
IPR001807	Chloride channel, voltage gated	PANTHER	PTHR11689	CHLORIDE CHANNEL	coord: 1..115 score: 8.7
IPR001807	Chloride channel, voltage gated	PFAM	PF00654	Voltage_CLC	coord: 1..44 score: 3.1
IPR014743	Chloride channel, core	GENE3D	1.10.3080.10	no description	coord: 1..67 score: 1.9
IPR014743	Chloride channel, core	SUPERFAMILY	81340	Clc chloride channel	coord: 1..66 score: 7
None	No IPR available	PANTHER	PTHR11689:SF11	CHLORIDE CHANNEL CLC, PLANT	coord: 1..115 score: 8.7
None	No IPR available	SEG	seg	seg	coord: 26..38 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720135 ID=GH720135; Name=GH720135; organism=Pisum sativum; type=EST; length=355bp
ACTACCCCATCGTCCATCACTGGGAAACCATTATGTGTAGTGTTTTTCAA
TACATCTACTATCTTTGCTACCTTTTCTACTCCTTGGAGGGAAATCACTG
CCGGCTTTACATCTACAAGCTCGCCAACAGTGAGGTTTCTCATCCATGAC
GCAGGATTTGCATCCATGAAAGGAAGGCCTTTCAAATGCAGTATAATCTC
ATAGATGCTAGGGTTGAAGCAATCTCCAACAGTTTTGGCTATTAGGAGAA
CAATCATTGTTATTGGTAGTAGGAGAAGATTGCTAGTAAGTTCAAGGAAG
ATCACGCAAAGGGACACAGTCATCCTCATCGAACCAGCCATGAGGGAAGC
TGCAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005247	voltage-gated chloride channel activity
GO:0005216	monoatomic ion channel activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006821	chloride transport
GO:0055085	transmembrane transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR001807	Cl-channel_volt-gated
IPR014743	Cl-channel_core

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