GH720474

EST Overview


Name	GH720474
Unique Name	GH720474
Type	EST
Organism	Pisum sativum (pea)
Sequence length	263

Libraries


Library Name	Type
LIBEST_024271 552NI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720474 vs. TrEMBL
Match: Q570Q7_ARATH (60S ribosomal protein L7A OS=Arabidopsis thaliana GN=At2g47610 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.539e-12
Identity = 35/37 (94.59%), Postives = 37/37 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRM 132
            +YEEYRKKWGGGIMGSKSQAKTKAKER+IAKEAAQRM
Sbjct:   53 KYEEYRKKWGGGIMGSKSQAKTKAKERVIAKEAAQRM 89

[Close]

BLAST of GH720474 vs. TrEMBL
Match: D7SMN0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_83.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036540001 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.648e-12
Identity = 33/38 (86.84%), Postives = 38/38 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRMT 135
            +Y+EYRK+WGGGIMGSKSQAKTKAKE+LIAKEAAQRM+
Sbjct:  300 KYDEYRKRWGGGIMGSKSQAKTKAKEKLIAKEAAQRMS 337

[Close]

BLAST of GH720474 vs. TrEMBL
Match: D7LTA6_ARALY (60S ribosomal protein L7A OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_486792 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.648e-12
Identity = 33/37 (89.19%), Postives = 37/37 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRM 132
            +YEEYRKKWGGGIMGSKSQAKTKAKER++AKEAAQR+
Sbjct:  219 KYEEYRKKWGGGIMGSKSQAKTKAKERVLAKEAAQRL 255

[Close]

BLAST of GH720474 vs. TrEMBL
Match: D7LGP8_ARALY (60S ribosomal protein L7A OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_483941 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.648e-12
Identity = 33/37 (89.19%), Postives = 37/37 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRM 132
            +YEEYRKKWGGGIMGSKSQAKTKAKER++AKEAAQR+
Sbjct:  220 KYEEYRKKWGGGIMGSKSQAKTKAKERVLAKEAAQRL 256

[Close]

BLAST of GH720474 vs. TrEMBL
Match: A5BD49_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030542 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.648e-12
Identity = 33/38 (86.84%), Postives = 38/38 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRMT 135
            +Y+EYRK+WGGGIMGSKSQAKTKAKE+LIAKEAAQRM+
Sbjct:  221 KYDEYRKRWGGGIMGSKSQAKTKAKEKLIAKEAAQRMS 258

[Close]

BLAST of GH720474 vs. TrEMBL
Match: D7SVM2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_68.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033050001 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.149e-11
Identity = 33/38 (86.84%), Postives = 37/38 (97.37%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRMT 135
            +Y+E RKKWGGGIMGSKSQA+TKAKERL+AKEAAQRMT
Sbjct:  324 KYDENRKKWGGGIMGSKSQARTKAKERLVAKEAAQRMT 361

[Close]

BLAST of GH720474 vs. TrEMBL
Match: C6TBY0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.149e-11
Identity = 32/37 (86.49%), Postives = 37/37 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRM 132
            +Y+EYRKKWGGGIMGSKSQAKT+AKE+L+AKEAAQRM
Sbjct:  223 KYDEYRKKWGGGIMGSKSQAKTRAKEKLLAKEAAQRM 259

[Close]

BLAST of GH720474 vs. TrEMBL
Match: Q42245_ARATH (Ribosomal protein (Fragment) OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.666e-11
Identity = 34/40 (85.00%), Postives = 37/40 (92.50%), Query Frame = 1

Query:   13 SRPRYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRM 132
            S  RYE+ RKKWGGG+MGSKSQAKTKAKER+IAKEAAQRM
Sbjct:    3 STTRYEDDRKKWGGGLMGSKSQAKTKAKERVIAKEAAQRM 42

[Close]

BLAST of GH720474 vs. TrEMBL
Match: B9GF57_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_797956 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.666e-11
Identity = 31/38 (81.58%), Postives = 38/38 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRMT 135
            +Y+EYRKKWGGGIMGSKSQAK+KAK++++AKEAAQRMT
Sbjct:  215 KYDEYRKKWGGGIMGSKSQAKSKAKDKVLAKEAAQRMT 252

[Close]

BLAST of GH720474 vs. TrEMBL
Match: B9SIW8_RICCO (60S ribosomal protein L7a, putative OS=Ricinus communis GN=RCOM_0790710 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.254e-11
Identity = 31/38 (81.58%), Postives = 38/38 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRMT 135
            +++E+RK+WGGGIMGSKSQAKTKAKER++AKEAAQRMT
Sbjct:  221 KFDEHRKRWGGGIMGSKSQAKTKAKERVLAKEAAQRMT 258

[Close]

BLAST of GH720474 vs. SwissProt
Match: RL7A2_ARATH (60S ribosomal protein L7a-2 OS=Arabidopsis thaliana GN=RPL7AB PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.265e-13
Identity = 35/37 (94.59%), Postives = 37/37 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRM 132
            +YEEYRKKWGGGIMGSKSQAKTKAKER+IAKEAAQRM
Sbjct:  219 KYEEYRKKWGGGIMGSKSQAKTKAKERVIAKEAAQRM 255

[Close]

BLAST of GH720474 vs. SwissProt
Match: RL7A1_ARATH (60S ribosomal protein L7a-1 OS=Arabidopsis thaliana GN=RPL7AA PE=2 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 1.265e-13
Identity = 35/37 (94.59%), Postives = 37/37 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRM 132
            +YEEYRKKWGGGIMGSKSQAKTKAKER+IAKEAAQRM
Sbjct:  220 KYEEYRKKWGGGIMGSKSQAKTKAKERVIAKEAAQRM 256

[Close]

BLAST of GH720474 vs. SwissProt
Match: RL7A_ORYSJ (60S ribosomal protein L7a OS=Oryza sativa subsp. japonica GN=RPL7A PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 9.068e-12
Identity = 30/38 (78.95%), Postives = 37/38 (97.37%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRMT 135
            +++E RKKWGGG+MGSKSQAKTKA+E+L+AKEAAQRMT
Sbjct:  221 KFDEVRKKWGGGVMGSKSQAKTKAREKLLAKEAAQRMT 258

[Close]

BLAST of GH720474 vs. TAIR peptide
Match: AT3G62870.1 (| Symbols: | Ribosomal protein L7Ae/L30e/S12e/Gadd45 family protein | chr3:23242862-23244273 REVERSE LENGTH=256)

HSP 1 Score: 75.0998 bits (183), Expect = 9.894e-15
Identity = 35/37 (94.59%), Postives = 37/37 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRM 132
            +YEEYRKKWGGGIMGSKSQAKTKAKER+IAKEAAQRM
Sbjct:  219 KYEEYRKKWGGGIMGSKSQAKTKAKERVIAKEAAQRM 255

[Close]

BLAST of GH720474 vs. TAIR peptide
Match: AT2G47610.1 (| Symbols: | Ribosomal protein L7Ae/L30e/S12e/Gadd45 family protein | chr2:19529854-19531401 FORWARD LENGTH=257)

HSP 1 Score: 75.0998 bits (183), Expect = 9.894e-15
Identity = 35/37 (94.59%), Postives = 37/37 (100.00%), Query Frame = 1

Query:   22 RYEEYRKKWGGGIMGSKSQAKTKAKERLIAKEAAQRM 132
            +YEEYRKKWGGGIMGSKSQAKTKAKER+IAKEAAQRM
Sbjct:  220 KYEEYRKKWGGGIMGSKSQAKTKAKERVIAKEAAQRM 256

The following BLAST results are available for this feature:

BLAST of GH720474 vs. TrEMBL
BLAST of GH720474 vs. SwissProt
BLAST of GH720474 vs. TAIR peptide

BLAST of GH720474 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q570Q7_ARATH	2.539e-12	94.59	60S ribosomal protein L7A OS=Arabidopsis thaliana ...	[more]
D7SMN0_VITVI	9.648e-12	86.84	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7LTA6_ARALY	9.648e-12	89.19	60S ribosomal protein L7A OS=Arabidopsis lyrata su...	[more]
D7LGP8_ARALY	9.648e-12	89.19	60S ribosomal protein L7A OS=Arabidopsis lyrata su...	[more]
A5BD49_VITVI	9.648e-12	86.84	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7SVM2_VITVI	2.149e-11	86.84	Whole genome shotgun sequence of line PN40024, sca...	[more]
C6TBY0_SOYBN	2.149e-11	86.49	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q42245_ARATH	3.666e-11	85.00	Ribosomal protein (Fragment) OS=Arabidopsis thalia...	[more]
B9GF57_POPTR	3.666e-11	81.58	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SIW8_RICCO	6.254e-11	81.58	60S ribosomal protein L7a, putative OS=Ricinus com...	[more]

BLAST of GH720474 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
RL7A2_ARATH	1.265e-13	94.59	60S ribosomal protein L7a-2 OS=Arabidopsis thalian...	[more]
RL7A1_ARATH	1.265e-13	94.59	60S ribosomal protein L7a-1 OS=Arabidopsis thalian...	[more]
RL7A_ORYSJ	9.068e-12	78.95	60S ribosomal protein L7a OS=Oryza sativa subsp. j...	[more]

BLAST of GH720474 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT3G62870.1	9.894e-15	94.59	\| Symbols: \| Ribosomal protein L7Ae/L30e/S12e/Gad...	[more]
AT2G47610.1	9.894e-15	94.59	\| Symbols: \| Ribosomal protein L7Ae/L30e/S12e/Gad...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 37..50 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720474 ID=GH720474; Name=GH720474; organism=Pisum sativum; type=EST; length=263bp
CGATTAGCGTGGTCGCGGCCGAGGTACGAAGAATACAGGAAGAAGTGGGG
TGGCGGTATCATGGGTTCTAAATCTCAAGCAAAAACCAAGGCGAAGGAGA
GACTCATTGCCAAGGAAGCTGCTCAAAGAATGACCTAGAAGTTTCATATG
CTTTGGTTATTTTAGAATTTGTGAGGTTGTTATTCTTCTATATTTTTGAT
GGAGGTTTTTCTTTAATTCTGGGAGTTGTAATCTACAATCACTGGTACCT
GCCCGGGCGGCCG

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