Identification and Verification of QTL Associated with Frost Tolerance Using Linkage Mapping and GWAS in Winter Faba Bean

Publication Overview
TitleIdentification and Verification of QTL Associated with Frost Tolerance Using Linkage Mapping and GWAS in Winter Faba Bean
AuthorsSallam A, Arbaoui M, El-Esawi M, Abshire N, Martsch R
TypeJournal Article
Journal NameFrontiers in plant science
CitationSallam A, Arbaoui M, El-Esawi M, Abshire N, Martsch R. Identification and Verification of QTL Associated with Frost Tolerance Using Linkage Mapping and GWAS in Winter Faba Bean. Frontiers in plant science. 2016; 7:1098.


Frost stress is one of the abiotic stresses that causes a significant reduction in winter faba bean yield in Europe. The main objective of this work is to genetically improve frost tolerance in winter faba bean by identifying and validating QTL associated with frost tolerance to be used in marker-assisted selection (MAS). Two different genetic backgrounds were used: a biparental population (BPP) consisting of 101 inbred lines, and 189 genotypes from single seed descent (SSD) from the Gottingen Winter bean Population (GWBP). All experiments were conducted in a frost growth chamber under controlled conditions. Both populations were genotyped using the same set of 189 SNP markers. Visual scoring for frost stress symptoms was used to define frost tolerance in both populations. In addition, leaf fatty acid composition (FAC) and proline content were analyzed in BPP as physiological traits. QTL mapping (for BPP) and genome wide association studies (for GWBP) were performed to detect QTL associated with frost tolerance. High genetic variation between genotypes, and repeatability estimates, were found for all traits. QTL mapping and GWAS identified new putative QTL associated with promising frost tolerance and related traits. A set of 54 SNP markers common in both genetic backgrounds showed a high genetic diversity with polymorphic information content (PIC) ranging from 0.31 to 0.37 and gene diversity ranging from 0.39 to 0.50. This indicates that these markers may be polymorphic for many faba bean populations. Five SNP markers showed a significant marker-trait association with frost tolerance and related traits in both populations. Moreover, synteny analysis between Medicago truncatula (a model legume) and faba bean genomes was performed to identify candidate genes for these markers. Collinearity was evaluated between the faba bean genetic map constructed in this study and the faba bean consensus map, resulting in identifying possible genomic regions in faba bean which may control frost tolerance genes. The two genetic backgrounds were useful in detecting new variation for improving frost tolerance in winter faba bean. Of the five validated SNP markers, one (VF_Mt3g086600) was found to be associated with frost tolerance and FAC in both populations. This marker was also associated with winter hardiness and high yield in earlier studies. This marker is located in a gene of unknown function.

This publication contains information about 12 features:
Feature NameUniquenameType
Linoleic acid contentqLAC.Cote_d'Or1_x_BeanPureLine4628.LG2.hardenedQTL
Linolenic acid contentqLNAC.Cote_d'Or1_x_BeanPureLine4628.LG3.hardenedQTL
Oleic acid contentqOAC.Cote_d'Or1_x_BeanPureLine4628.LG10.hardenQTL
Oleic acid contentqOAC.Cote_d'Or1_x_BeanPureLine4628.LG2.hardenQTL
Oleic acid contentqOAC.Cote_d'Or1_x_BeanPureLine4628.LG3.hardenQTL
Palmitic acid contentqPAC.Cote_d'Or1_x_BeanPureLine4628.LG1.unhardenedQTL
Palmitic acid contentqPAC.Cote_d'Or1_x_BeanPureLine4628.LG3QTL
Palmitic acid contentqPAC.Cote_d'Or1_x_BeanPureLine4628.LG8.hardenedQTL
Palmitoleic acid contentqPLAC.Cote_d'Or1_x_BeanPureLine4628.LG3.unhardenedQTL
Stearic acid contentqSAC.Cote_d'Or1_x_BeanPureLine4628.LG3.hardenedQTL
Stearic acid contentqSAC.Cote_d'Or1_x_BeanPureLine4628.LG4.hardenedQTL
Stearidonic acid contentqSDAC.Cote_d'Or1_x_BeanPureLine4628.LG2.hardenedQTL
This publication contains information about 1 projects:
Project NameDescription
This publication contains information about 1 maps:
Map Name
faba-Côte d'Or 1_x_BeanPureLine4628-RIL-2016
This publication contains information about 4 stocks:
Stock NameUniquenameType
Côte d'Or 1Côte d'Or 1accession
BeanPureLine 4628BeanPureLine 4628accession
Côte d'Or 1_x_BeanPureLine4628Côte d'Or 1_x_BeanPureLine4628population
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
Publication Date2016
Journal AbbreviationFront Plant Sci
Language Abbreng
Publication TypeJournal Article
Journal CountrySwitzerland