Confirmation of QTLs controlling Ascochyta fabae resistance in different generations of faba bean (Vicia faba L.)

Publication Overview
TitleConfirmation of QTLs controlling Ascochyta fabae resistance in different generations of faba bean (Vicia faba L.)
AuthorsDíaz-Ruiz R, Satovic Z, Ávila C, Alfaro C, Gutierrez M, Torres A, Román B
TypeJournal Article
Journal NameCrop & pasture science
Volume60
Issue4
Year2009
Page(s)353-361
CitationDíaz-Ruiz R, Satovic Z, Ávila C, Alfaro C, Gutierrez M, Torres A, Román B. Confirmation of QTLs controlling Ascochyta fabae resistance in different generations of faba bean (Vicia faba L.). Crop & pasture science. 2009; 60(4):353-361.

Abstract

Ascochyta blight, caused by Ascochyta fabae Speg., is a disease of faba bean (Vicia faba L.) of worldwide distribution. In this study we have conducted an experiment on Ascochyta fabae resistance in 165 recombinant inbred lines (RILs) developed by single-seed descent from the cross between resistant and susceptible lines (Vf6xVf136) in which A. fabae resistance QTLs (quantitative trait loci) have been previously reported in the original F₂ population. Recombinant inbred lines were inoculated under controlled growth chamber conditions and evaluated for disease severity and infection type index. The linkage map was constructed by MAPMAKER V2.0 and the QTL analysis was carried out using QTL Cartographer. Two hundred and seventy-seven markers (238 RAPDs, 4 isozymes, 5 ESTs, 1 SCAR, 6 SSRs, 2 STSs, and 21 intron-spanning markers) mapped into 21 linkage groups covering 2.856.7cM, with a mean inter-marker distance of 12.72cM. Composite interval mapping identified two zones of putative QTL action in the RIL population for DSL (disease severity on leaves) and DSS (disease severity on stems) traits. Putative QTLs (Af1 and Af2) were identified on chromosome 3 and chromosome 2, respectively, and jointly explained 24% of the phenotypic variance of DSL and 16% of DSS. With this study we have (1) confirmed the QTLs for ascochyta blight resistance found in F₃ families in the derived RILs (F₆), (2) re-estimated their position and genetic effects, and (3) assessed the stability of these QTLs in different genetic backgrounds by comparison of the mapping data with a previous QTL study.
Features
This publication contains information about 18 features:
Feature NameUniquenameType
OPAD02OPAD02genetic_marker
OPAH14OPAH14genetic_marker
OPD15OPD15genetic_marker
OPF08OPF08genetic_marker
OPK03OPK03genetic_marker
OPK18OPK18genetic_marker
OPR16OPR16genetic_marker
OPS01OPS01genetic_marker
OPU10OPU10genetic_marker
OPW01OPW01genetic_marker
OPW07OPW07genetic_marker
PU11PU11genetic_marker
Ascochyta blight resistanceqABR.Vf6xVf136-F2.LGIIIA.Afl1.1QTL
Ascochyta blight resistanceqABR.Vf6xVf136-RIL.LGIIIA.Afl1.2QTL
Ascochyta blight resistanceqABR.Vf6xVf136-RIL.LGIIIA.Afl1.3QTL
Ascochyta blight resistanceqABR.Vf6xVf136-F2.LGIIA2.Afl2.1QTL
Ascochyta blight resistanceqABR.Vf6xVf136-RIL.LGIIA.Afl2.2QTL
Ascochyta blight resistanceqABR.Vf6xVf136-RIL.LGIIA.Afl2.3QTL
Projects
This publication contains information about 1 projects:
Project NameDescription
Faba-Ascochyta_Resistance-Diaz-Ruiz-2009
Featuremaps
This publication contains information about 2 maps:
Map Name
faba-Vf6xVf136-RIL-2009
faba-Vf6xVf136-F2-2009
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Published Location|||
Publication Date2009
URLhttp://dx.doi.org/10.1071/CP08190
Publication Model[electronic resource].
Language Abbreng
KeywordsAscochyta fabae, Vicia faba, blight, chromosome mapping, chromosomes, disease severity, expressed sequence tags, faba beans, foliar diseases, genetic background, growth chambers, inbred lines, isozymes, linkage groups, microsatellite repeats, quantitative trait loci, random amplified polymorphic DNA technique, stems, variance
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4222445