Discovery of molecular markers for Fusarium wilt via transcriptome sequencing of chickpea cultivars
Publication Overview
Abstract The wilt caused by Fusarium oxysporum
f.sp. Ciceris is a major problem for chickpea. The
large-scale discovery of DNA polymorphisms in
different cultivars can provide insights into the genetic
basis of phenotypic differences. In the present study,
we sequenced the transcriptomes of four chickpea
cultivars with contrasting response to Fusarium
infection, including susceptible JG62 and resistant/tolerant
ICCV2, K850 and WR315. Based on the analysis
of their transcriptome sequences, we identified at least
303 polymorphic microsatellites, of which 64 % could
be physically mapped on the chickpea genome.
Further, we identified a total of 14,462 single
nucleotide polymorphisms (SNPs) and 1864 insertions/deletions
(InDels) among the chickpea cultivars
analysed. More than 50 % of the SNPs and 19 % of the
InDels were detected in the predicted coding regions,
and about 46 % of these SNPs resulted in nonsynonymous
changes. In addition, we identified at least 60
large-effect SNPs and 248 InDels that affected the
integrity of the encoded protein. Several defencerelated
genes involved in different cellular processes
were found to harbour nonsynonymous and/or largeeffect
SNPs and/or InDels. These data will provide
functional markers and promising target genes for wilt
resistance and present a valuable resource for molecular
breeding for this important trait in chickpe
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