A CAPS marker set for mapping in linkage group III of pea (Pisum sativum L.)

Publication Overview
TitleA CAPS marker set for mapping in linkage group III of pea (Pisum sativum L.)
AuthorsKonovalov F, Toshchakova E, Gostimsky S
TypeJournal Article
Journal NameCellular & molecular biology letters
Volume10
Issue1
Year2005
Page(s)163-71
CitationKonovalov F, Toshchakova E, Gostimsky S. A CAPS marker set for mapping in linkage group III of pea (Pisum sativum L.). Cellular & molecular biology letters. 2005; 10(1):163-71.

Abstract

A set of twelve CAPS markers was mapped for linkage group III of pea (Pisum sativum L.). New primers were designed to use a polymerase chain reaction to amplify fragments of sequenced pea genes containing at least one large intron. Amplification products were tested for polymorphism across three pea lines (Chi115, Flagman and WL1238) using eleven four-base restriction endonucleases. Nine STS markers for linkage group III from the literature were also tested for polymorphism, and five of these were used in this mapping study as anchor points. All polymorphic loci were located by genetic analysis of the F(2)population from the cross Chi115 x WL1238, and a map of linkage group III consisting of one morphological and twelve CAPS markers was created. The map covers the full length of the chromosome and is about 162 cM long. All the CAPS markers in a set were used to test for polymorphism among 10 additional pea DNA samples extracted from different marker lines and cultivars.

Features
This publication contains information about 6 features:
Feature NameUniquenameType
GprAIIGprAIIgenetic_marker
GprBGprBgenetic_marker
PhLypCPhLypCgenetic_marker
PK4PK4genetic_marker
Adh1Adh1genetic_marker
Egl1_CAPSEgl1_CAPSgenetic_marker
Featuremaps
This publication contains information about 1 maps:
Map Name
pea-Chi115xWL1238-F2
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint
ISSN1425-8153
pISSN1425-8153
Publication Date2005
Journal AbbreviationCell. Mol. Biol. Lett.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryPoland
Publication TypeResearch Support, Non-U.S. Gov't
Cross References
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DatabaseAccession
PMID: PubMedPMID:15809687