Pisum sativum unigene v1
An assembly of 18,552 EST sequences downloaded from NCBI dbEST using the cap3 assembly software (parameter: -p 90). 17,717 ESTs were used in the analysis after trimming using crossmatch against UniVec_Core (parameter: -minmatch 10 -penalty -2 -minscore 20 -screen) and filtering out rRNA/tRNA contamination using tblastx (parameter: -e 1e-6). Poly-A tail was also trimmed after RNA filtering. The assembly results in 3,095 contigs and 6,950 singlets.
Additional information about this analysis:
Homology Analysis Reports: