Use of synteny to identify candidate genes underlying QTL controlling stomatal traits in faba bean (Vicia faba L.)

Publication Overview
TitleUse of synteny to identify candidate genes underlying QTL controlling stomatal traits in faba bean (Vicia faba L.)
AuthorsKhazaei H, O’Sullivan DM, Sillanpää MJ, Stoddard FL
TypeJournal Article
Journal NameTheoretical and applied genetics
CitationKhazaei H, OSullivan DM, Sillanpää MJ, Stoddard FL. Use of synteny to identify candidate genes underlying QTL controlling stomatal traits in faba bean (Vicia faba L.). Theoretical and applied genetics. 2014; 127(11):2371-2385.


KEY MESSAGE : We have identified QTLs for stomatal characteristics on chromosome II of faba bean by applying SNPs derived from M. truncatula , and have identified candidate genes within these QTLs using synteny between the two species. Faba bean (Vicia faba L.) is a valuable food and feed crop worldwide, but drought often limits its production, and its genome is large and poorly mapped. No information is available on the effects of genomic regions and genes on drought adaptation characters such as stomatal characteristics in this species, but the synteny between the sequenced model legume, Medicago truncatula, and faba bean can be used to identify candidate genes. A mapping population of 211 F₅ recombinant inbred lines (Mélodie/2 × ILB 938/2) were phenotyped to identify quantitative trait loci (QTL) affecting stomatal morphology and function, along with seed weight, under well-watered conditions in a climate-controlled glasshouse in 2013 and 2014. Canopy temperature (CT) was evaluated in 2013 under water-deficit (CTd). In total, 188 polymorphic single nucleotide polymorphisms (SNPs), developed from M. truncatula genome data, were assigned to nine linkage groups that covered ~928 cM of the faba bean genome with an average inter-marker distance of 5.8 cM. 15 putative QTLs were detected, of which eight (affecting stomatal density, length and conductance and CT) co-located on chromosome II, in the vicinity of a possible candidate gene—a receptor-like protein kinase found in the syntenic interval of M. truncatula chromosome IV. A ribose-phosphate pyrophosphokinase from M. truncatula chromosome V, postulated as a possible candidate gene for the QTL for CTd, was found some distance away in the same chromosome. These results demonstrate that genomic information from M. truncatula can successfully be translated to the faba bean genome.
This publication contains information about 235 features:
Feature NameUniquenameType
Stomatal conductanceqSTCC.MelodiexILB938.LG4.2013QTL
Stomatal conductanceqSTCC.MelodiexILB938.LG4.2014QTL
Canopy temperatureqCANTMP.MelodiexILB938.LG4.2012QTL
Canopy temperatureqCANTMP.MelodiexILB938.LG4.2013QTL
Canopy temperatureqCANTMP.MelodiexILB938.LG4.2014QTL
Canopy temperature under water stressqCANH2O.MelodiexILB938.LG4.2013QTL
10-seed weight per plantqTNSDWT.MelodiexILB938.LG4.2013.1QTL
10-seed weight per plantqTNSDWT.MelodiexILB938.LG6.2013.2QTL
10-seed weight per plantqTNSDWT.MelodiexILB938.LG4.2014.1QTL
10-seed weight per plantqTNSDWT.MelodiexILB938.LG6.2014.2QTL


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Language Abbreng
Publication Date2014
Publication Model[electronic resource].
Publication TypeJournal Article
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KeywordsMedicago truncatula, Vicia faba, canopy, chromosomes, drought, faba beans, genes, genomics, inbred lines, linkage groups, protein kinases, quantitative trait loci, single nucleotide polymorphism, temperature, translation (genetics)