FE673309

EST Overview
NameFE673309
Unique NameFE673309
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length207
Libraries
Library NameType
LIBEST_022820 Drought-stressed chickpea leave librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of FE673309 vs. TrEMBL
Match: Q76KV8_PEA (Short-chain alcohol dehydrogenase A (Fragment) OS=Pisum sativum GN=sadA PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.630e-13
Identity = 37/58 (63.79%), Postives = 43/58 (74.14%), Query Frame = 3 
Query:   33 AEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            A Q A +VVI DIQ+ELG Q+  SIGT RCT+IHCD+  ED  KNLVQSTV T G +D
Sbjct:   45 ANQAARMVVIADIQDELGIQVAESIGTDRCTFIHCDIRIEDDVKNLVQSTVDTYGQID 102

HSP 2 Score: 22.3274 bits (46), Expect = 4.630e-13
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:    2 GIGKKTAHVF 31
            GIGK+TAH+F
Sbjct:   35 GIGKETAHLF 44
[Close]
BLAST of FE673309 vs. TrEMBL
Match: Q9SQJ3_PEA (Short-chain alcohol dehydrogenase OS=Pisum sativum GN=sadA PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.634e-13
Identity = 37/58 (63.79%), Postives = 43/58 (74.14%), Query Frame = 3 
Query:   33 AEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            A Q A +VVI DIQ+ELG Q+  SIGT RCT+IHCD+  ED  KNLVQSTV T G +D
Sbjct:   36 ANQAARMVVIADIQDELGIQVAESIGTDRCTFIHCDIRIEDDVKNLVQSTVDTYGQID 93

HSP 2 Score: 22.3274 bits (46), Expect = 4.634e-13
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:    2 GIGKKTAHVF 31
            GIGK+TAH+F
Sbjct:   26 GIGKETAHLF 35
[Close]
BLAST of FE673309 vs. TrEMBL
Match: Q9SQF9_PEA (Short-chain alcohol dehydrogenase SAD-C OS=Pisum sativum GN=sadC PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.634e-13
Identity = 37/58 (63.79%), Postives = 43/58 (74.14%), Query Frame = 3 
Query:   33 AEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            A Q A +VVI DIQ+ELG Q+  SIGT RCT+IHCD+  ED  KNLVQSTV T G +D
Sbjct:   36 ANQAARMVVIADIQDELGIQVAESIGTDRCTFIHCDIRIEDDVKNLVQSTVDTYGQID 93

HSP 2 Score: 22.3274 bits (46), Expect = 4.634e-13
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:    2 GIGKKTAHVF 31
            GIGK+TAH+F
Sbjct:   26 GIGKETAHLF 35
[Close]
BLAST of FE673309 vs. TrEMBL
Match: B9IEG2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_666348 PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.135e-11
Identity = 33/58 (56.90%), Postives = 46/58 (79.31%), Query Frame = 3 
Query:   33 AEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            A+ GA +VVI DIQ+ELG+Q+  SIG Q+C+Y+HCDV +E+Q K+LV+ TVK  G +D
Sbjct:   37 AQHGALIVVIADIQDELGHQVATSIGQQKCSYMHCDVTDEEQVKSLVEWTVKNFGRLD 94
[Close]
BLAST of FE673309 vs. TrEMBL
Match: B9I3Y6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_834366 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.211e-11
Identity = 34/61 (55.74%), Postives = 45/61 (73.77%), Query Frame = 3 
Query:   24 MSLAEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            ++ AE GA  VVI DIQ+E G ++  SIGT R TYIHCDV +E+Q K+LV+STV+  G +D
Sbjct:   31 LAFAENGARAVVIADIQDEKGQKLAESIGTNRSTYIHCDVGDENQVKSLVESTVQLYGHLD 91
[Close]
BLAST of FE673309 vs. TrEMBL
Match: B9R941_RICCO (Short chain alcohol dehydrogenase, putative OS=Ricinus communis GN=RCOM_1513550 PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.059e-10
Identity = 30/59 (50.85%), Postives = 45/59 (76.27%), Query Frame = 3 
Query:   30 LAEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            LA+ GA +VVI DIQ+++G  +  SIGT +C+Y+HCDV  E+Q K+LV+ TV++ G +D
Sbjct:   31 LADHGASMVVIADIQDQVGQDVATSIGTNKCSYVHCDVTKEEQVKSLVEWTVQSFGKLD 89
[Close]
BLAST of FE673309 vs. TrEMBL
Match: D7SIG8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_0.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00008067001 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.360e-10
Identity = 30/58 (51.72%), Postives = 43/58 (74.14%), Query Frame = 3 
Query:   33 AEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            +E GA  ++I DIQ+ELG  + +SIG+  CT+IHCDV NEDQ K++V+ TV+  G +D
Sbjct:   34 SEHGARAIIIADIQDELGQNLASSIGSHFCTFIHCDVTNEDQVKSMVEWTVQKYGQLD 91
[Close]
BLAST of FE673309 vs. TrEMBL
Match: A5C5T3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_039801 PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.360e-10
Identity = 30/58 (51.72%), Postives = 43/58 (74.14%), Query Frame = 3 
Query:   33 AEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            +E GA  ++I DIQ+ELG  + +SIG+  CT+IHCDV NEDQ K++V+ TV+  G +D
Sbjct:  200 SEHGARAIIIADIQDELGQNLASSIGSHFCTFIHCDVTNEDQVKSMVEWTVQKYGQLD 257

HSP 2 Score: 67.781 bits (164), Expect = 4.026e-10
Identity = 32/58 (55.17%), Postives = 40/58 (68.97%), Query Frame = 3 
Query:   33 AEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            A+ GA  VV+ DIQ+ELG  +  SIG  RC YIHCDV +E Q K +V+STVK  G +D
Sbjct:   34 ADHGARAVVVADIQDELGRGVAESIGLHRCRYIHCDVTDEQQIKAMVESTVKMFGQLD 91
[Close]
BLAST of FE673309 vs. TrEMBL
Match: B9IFJ9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_666749 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.083e-10
Identity = 33/61 (54.10%), Postives = 44/61 (72.13%), Query Frame = 3 
Query:   24 MSLAEQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            ++  E GA  VVI DIQ+E G ++  SIGT R TYIHCDV +E+Q K+LV+STV+  G +D
Sbjct:   31 LAFVENGARGVVIADIQDEKGQKLAESIGTNRSTYIHCDVTDENQVKSLVESTVQLYGQLD 91
[Close]
BLAST of FE673309 vs. TrEMBL
Match: Q9SQJ2_PEA (Short-chain alcohol dehydrogenase OS=Pisum sativum GN=sadB PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 4.026e-10
Identity = 32/57 (56.14%), Postives = 41/57 (71.93%), Query Frame = 3 
Query:   36 EQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            +QGA +VVI DIQ++LG Q+  SIGT +C +IHCD+  ED  KNLVQ TV   G +D
Sbjct:   25 KQGARIVVIADIQDKLGIQVAESIGTDKCRFIHCDIRIEDDVKNLVQLTVDCYGQID 81
[Close]
BLAST of FE673309 vs. TAIR peptide
Match: AT2G47140.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr2:19350970-19352059 REVERSE LENGTH=257)

HSP 1 Score: 55.4546 bits (132), Expect = 8.234e-9
Identity = 26/57 (45.61%), Postives = 38/57 (66.67%), Query Frame = 3 
Query:   36 EQGAGVVVIPDIQNELGNQMVASIGTQRCTYIHCDVANEDQFKNLVQSTVKT*GPVD 206
            E GA VV++ D+Q+ELG  +  SIG  + +Y HCDV NE + +N V+ TV+  G +D
Sbjct:   30 EHGARVVIV-DVQDELGQNVAVSIGEDKASYYHCDVTNETEVENAVKFTVEKYGKLD 85
The following BLAST results are available for this feature:
BLAST of FE673309 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q76KV8_PEA4.630e-1363.79Short-chain alcohol dehydrogenase A (Fragment) OS=... [more]
Q9SQJ3_PEA4.634e-1363.79Short-chain alcohol dehydrogenase OS=Pisum sativum... [more]
Q9SQF9_PEA4.634e-1363.79Short-chain alcohol dehydrogenase SAD-C OS=Pisum s... [more]
B9IEG2_POPTR2.135e-1156.90Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9I3Y6_POPTR6.211e-1155.74Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9R941_RICCO1.059e-1050.85Short chain alcohol dehydrogenase, putative OS=Ric... [more]
D7SIG8_VITVI2.360e-1051.72Whole genome shotgun sequence of line PN40024, sca... [more]
A5C5T3_VITVI2.360e-1051.72Putative uncharacterized protein OS=Vitis vinifera... [more]
B9IFJ9_POPTR3.083e-1054.10Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q9SQJ2_PEA4.026e-1056.14Short-chain alcohol dehydrogenase OS=Pisum sativum... [more]
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BLAST of FE673309 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT2G47140.18.234e-945.61| Symbols: | NAD(P)-binding Rossmann-fold superfa... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002198Short-chain dehydrogenase/reductase SDRPANTHERPTHR19410SHORT-CHAIN DEHYDROGENASES/REDUCTASE FAMILY MEMBERcoord: 11..62
score: 3.5
IPR016040NAD(P)-binding domainGENE3D3.40.50.720no descriptioncoord: 9..62
score: 5.1
NoneNo IPR availablePANTHERPTHR19410:SF80SHORT CHAIN ALCOHOL DEHYDROGENASEcoord: 11..62
score: 3.5

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FE673309 ID=FE673309; Name=FE673309; organism=Cicer arietinum; type=EST; length=207bp
CGGCATCGGCAAAAAGACCGCACATGTCTTTGGCCGAACAAGGTGCAGGT
GTGGTCGTCATACCTGACATCCAAAACGAGTTGGGTAATCAAATGGTTGC
GTCCATTGGCACTCAAAGGTGTACCTACATTCACTGTGACGTGGCAAACG
AAGATCAATTTAAAAATCTTGTCCAATCAACCGTCAAAACTTAGGGACCG
GTGGATA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0003824catalytic activity
GO:0005488binding
Vocabulary: INTERPRO
TermDefinition
IPR002198DH_sc/Rdtase_SDR
IPR016040NAD(P)-bd_dom