GR395259

EST Overview


Name	GR395259
Unique Name	GR395259
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	427

Libraries


Library Name	Type
LIBEST_024780 ICC1882 PEG induced drought cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMYA_VIGMU (Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 4.823e-21
Identity = 52/101 (51.49%), Postives = 69/101 (68.32%), Query Frame = -3

Query:  132 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVW 422
            VM+GYAYILTHPGTPSIFYDHF+DW   + EQI KL   R R GI+ +S ++I+ ++ +LY A I  K+ +KIG     G+  PS     ++TSG +YAVW
Sbjct:  324 VMQGYAYILTHPGTPSIFYDHFFDW--GLKEQIAKLSSIRLRNGINEKSTVKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLH--VATSGQDYAVW 420

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMY2_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 99.3673 bits (246), Expect = 6.298e-21
Identity = 50/103 (48.54%), Postives = 70/103 (67.96%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVWHK 422
            VM+GYAYILTHPGTP IFYDHF+DW   + E+I +L+  R R GIH+ S ++I+EA  +LY A I  K+ +K+G     G+  P G  + ++  G++YAVW K
Sbjct:  328 VMQGYAYILTHPGTPCIFYDHFFDW--GLKEEIDRLVSVRTRHGIHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGG--FKVAAHGNDYAVWEK 426

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMY6_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.6 PE=2 SV=2)

HSP 1 Score: 97.8265 bits (242), Expect = 1.833e-20
Identity = 51/103 (49.51%), Postives = 68/103 (66.02%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVWHK 422
            VM+GYAYILTHPG P IFYDHF+DW   + E+I +L+  R RQGIHS S ++I+EA  +LY A I  K+ +K+G     G   P G  + +   G++YAVW K
Sbjct:  330 VMQGYAYILTHPGNPCIFYDHFFDW--GLKEEIDRLVSIRTRQGIHSESKLQIMEADADLYLAEIEGKVIVKLGPRYDVGHLIPEG--FKVVAHGNDYAVWEK 428

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMY4_HORVU (Alpha-amylase type B isozyme (Fragment) OS=Hordeum vulgare GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.188e-19
Identity = 50/103 (48.54%), Postives = 67/103 (65.05%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVWHK 422
            VM+GYAYILTHP TP IFYDHF+DW     E+I +L+  R R GIH+ S ++I+EA  +LY A I  K+ +K+G     G+  P G E   +  G++YAVW K
Sbjct:   54 VMQGYAYILTHPRTPCIFYDHFFDWGPK--EEIDRLVSVRTRHGIHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFEG--AAHGNDYAVWEK 152

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMY1_ORYSJ (Alpha-amylase OS=Oryza sativa subsp. japonica GN=AMY1.1 PE=2 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 2.646e-19
Identity = 50/103 (48.54%), Postives = 63/103 (61.17%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVWHK 422
            VM+GYAYILTHPG P IFYDHF+DW   + E+I +L+  R RQGIH  S +RI+EA  +LY A I  K+  KIG         P G  + +   G  YA+W K
Sbjct:  335 VMQGYAYILTHPGNPCIFYDHFFDW--GLKEEIERLVSIRNRQGIHPASELRIMEADSDLYLAEIDGKVITKIGPRYDVEHLIPEG--FQVVAHGDGYAIWEK 433

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMY5_HORVU (Alpha-amylase type B isozyme (Fragment) OS=Hordeum vulgare GN=AMY1.5 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.313e-18
Identity = 49/103 (47.57%), Postives = 66/103 (64.08%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVWHK 422
            VM+GYAYILTH GTP IFYDHF+DW     E+I +L+    R GIHS S ++I+EA  +L  A I  K+ +K+G     G+  P G E  ++  G++YAVW K
Sbjct:   36 VMQGYAYILTHQGTPCIFYDHFFDWGPK--EEIDRLVSVSTRHGIHSESKLQIIEADADLCLAEIDGKVIVKLGPRYDVGNLIPGGFE--VAAHGNDYAVWEK 134

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMY3_WHEAT (Alpha-amylase AMY3 OS=Triticum aestivum GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.715e-18
Identity = 49/104 (47.12%), Postives = 64/104 (61.54%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD-----GSWCPSGREWTLSTSGHNYAVWHK 422
            VM+GYAYILTHPG P IFYDH +DW   + ++I  L   R R GIH  S + IL+A+ +LY A IG K+  KIG       +  PSG  + ++  G+NY VW K
Sbjct:  311 VMQGYAYILTHPGIPCIFYDHVFDW--KLKQEITALATVRSRNGIHPGSTLDILKAEGDLYVAKIGGKVITKIGSRYNIGDNVIPSG--FKIAAKGNNYCVWEK 410

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMY3C_ORYSJ (Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica GN=AMY1.7 PE=2 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 2.926e-18
Identity = 48/103 (46.60%), Postives = 64/103 (62.14%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVWHK 422
            VM+GYAYILTHPG P IFYDH +DWN  + ++I  L   R R GIH  S + IL A  ++Y A+I +K+  KIG     G+  PS  ++ +   G+NY VW K
Sbjct:  328 VMQGYAYILTHPGVPCIFYDHVFDWN--LKQEISTLAAVRSRNGIHPGSKLNILAADGDVYVAMIDDKVITKIGTRYDVGNLIPS--DFHVVAHGNNYCVWEK 426

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMY3E_ORYSJ (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 6.518e-18
Identity = 46/103 (44.66%), Postives = 65/103 (63.11%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVWHK 422
            VM+GYAYILTHPG P IFYDH +DWN  +  +I  L + R R GIH+ S + IL+A+ ++Y A+I  K+  K+G     G   PS  ++ +   G++Y VW K
Sbjct:  327 VMQGYAYILTHPGIPCIFYDHVFDWN--LQHEIATLAEIRSRNGIHAESTLDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPS--DFHVVAHGNDYCVWEK 425

[Close]

BLAST of GR395259 vs. SwissProt
Match: AMY3A_ORYSJ (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 6.518e-18
Identity = 46/103 (44.66%), Postives = 68/103 (66.02%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVWHK 422
            V+ GYAYILTHPG P IFYDH +DWN  + ++I  L  TRKR GI++ S +R+L A+ ++Y A++ E++  KIG     G+  PS  ++ +   G++Y VW K
Sbjct:  330 VILGYAYILTHPGVPCIFYDHVFDWN--LKQEINALAATRKRNGINAGSKLRVLAAESDMYVAMVDERVITKIGPRIDVGNIIPS--DFHIVAHGNDYCVWEK 428

[Close]

BLAST of GR395259 vs. TrEMBL
Match: C6TCX9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 4.207e-47
Identity = 82/99 (82.83%), Postives = 93/99 (93.94%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            +MEGYAYILTHPG P++FYDHFYDW +S+ EQIVKLID RKRQGI SRS +RILEAKH+LYSA+IGEK+CMKIG+GSWCP+GREWTLSTSGHNYAVWHK
Sbjct:  315 IMEGYAYILTHPGIPTVFYDHFYDWGDSIREQIVKLIDVRKRQGIQSRSSVRILEAKHDLYSAVIGEKVCMKIGNGSWCPTGREWTLSTSGHNYAVWHK 413

[Close]

BLAST of GR395259 vs. TrEMBL
Match: Q9SGS0_ARATH (T23E18.6 OS=Arabidopsis thaliana PE=3 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 8.772e-45
Identity = 76/99 (76.77%), Postives = 91/99 (91.92%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            VMEGYAYILTHPG P +FYDHFYDW +S+H+QIVKLID R+RQ IHSRS +R+L+A+ NLY+AI+GEK+CMK+GDGSWCPSGR+WTL+TSGH YAVWHK
Sbjct:  314 VMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 412

[Close]

BLAST of GR395259 vs. TrEMBL
Match: Q8LFG1_ARATH (Alpha-amylase like protein OS=Arabidopsis thaliana GN=At1g76130 PE=2 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 8.772e-45
Identity = 76/99 (76.77%), Postives = 91/99 (91.92%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            VMEGYAYILTHPG P +FYDHFYDW +S+H+QIVKLID R+RQ IHSRS +R+L+A+ NLY+AI+GEK+CMK+GDGSWCPSGR+WTL+TSGH YAVWHK
Sbjct:  315 VMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of GR395259 vs. TrEMBL
Match: B9R8H8_RICCO (Alpha-amylase, putative OS=Ricinus communis GN=RCOM_1599860 PE=3 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 8.772e-45
Identity = 82/99 (82.83%), Postives = 90/99 (90.91%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            +MEGYAYILTHPG P++FYDHFYDW NS+HEQIVKLID RKRQ I+SRS IRILEA+ NLYSAIIGEK+CMKIGDGSW PSGREWTL+TSGH YAVW K
Sbjct:  300 IMEGYAYILTHPGIPTVFYDHFYDWGNSIHEQIVKLIDIRKRQDINSRSSIRILEAQPNLYSAIIGEKVCMKIGDGSWSPSGREWTLATSGHRYAVWQK 398

[Close]

BLAST of GR395259 vs. TrEMBL
Match: B9GQ54_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_798380 PE=3 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 8.772e-45
Identity = 79/99 (79.80%), Postives = 89/99 (89.90%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            +MEGYAY+LTHPG P++FYDHFYDW NS+HEQIVKLID RK Q IHSRS IRI EA+ NLYSAIIGEK+CMKIGDGSWCP+G+EWTL+TSGH YAVW K
Sbjct:  308 IMEGYAYLLTHPGMPTVFYDHFYDWGNSIHEQIVKLIDIRKHQDIHSRSSIRIFEAQPNLYSAIIGEKVCMKIGDGSWCPAGKEWTLATSGHRYAVWQK 406

[Close]

BLAST of GR395259 vs. TrEMBL
Match: D7KTA0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_316534 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.333e-44
Identity = 76/99 (76.77%), Postives = 90/99 (90.91%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            +MEGYAYILTHPG P +FYDHFYDW +S+H+QIVKLID R+RQ IHSRS IR+L+A+ NLY+AI+GEKLCMK+GDGSWCPSG +WTL+TSGH YAVWHK
Sbjct:  315 IMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTIRVLKAESNLYAAIVGEKLCMKLGDGSWCPSGGDWTLATSGHRYAVWHK 413

[Close]

BLAST of GR395259 vs. TrEMBL
Match: Q5BLY0_MALDO (Cytosolic alpha-amylase OS=Malus domestica GN=AMY9 PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.267e-43
Identity = 78/99 (78.79%), Postives = 89/99 (89.90%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            +MEGYAYIL HPG P++FYDHFYDW +S+H+QIVKLID RKRQ IHSRS I ILEA+ NLYSAIIGEK+CMKIGDGSWCP+ REWTL+TSGH YAVW+K
Sbjct:  316 IMEGYAYILMHPGIPTVFYDHFYDWGDSIHDQIVKLIDIRKRQDIHSRSSITILEAQPNLYSAIIGEKICMKIGDGSWCPASREWTLATSGHRYAVWNK 414

[Close]

BLAST of GR395259 vs. TrEMBL
Match: Q9M6R9_MALDO (Alpha-amylase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 1.654e-43
Identity = 77/99 (77.78%), Postives = 89/99 (89.90%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            +MEGY YILTHPG P++FYDHFYDW +S+H+QIVKLID RKRQ IHSRS I ILEA+ NLYSA+IGEK+CMKIGDGSWCP+GREWTL+T GH YAVW+K
Sbjct:  315 IMEGYTYILTHPGIPTVFYDHFYDWGDSIHDQIVKLIDIRKRQDIHSRSSITILEAQPNLYSAMIGEKVCMKIGDGSWCPAGREWTLATCGHRYAVWNK 413

[Close]

BLAST of GR395259 vs. TrEMBL
Match: Q5ZFR9_PLAMJ (Alpha-amylase OS=Plantago major GN=aamy1 PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 4.075e-42
Identity = 75/99 (75.76%), Postives = 87/99 (87.88%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            +MEGYAYILTHPG PS+FYDHFYDW  ++H+QIVKLID RK QGIHSRS I I+EA+ NLY+A IG+K+ MKIGDGSWCPSG+EWTL+TSGH YAVW K
Sbjct:  315 IMEGYAYILTHPGIPSVFYDHFYDWGTAIHDQIVKLIDIRKHQGIHSRSSIHIIEAQPNLYAANIGDKVSMKIGDGSWCPSGKEWTLATSGHRYAVWQK 413

[Close]

BLAST of GR395259 vs. TrEMBL
Match: D1MEA8_SOLTU (Alpha-amylase OS=Solanum tuberosum GN=amyA2 PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 1.186e-41
Identity = 76/99 (76.77%), Postives = 88/99 (88.89%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            VMEGYAYILTHPG PS+FYDHFY+W+NS+H+QIVKLI  R+ QGIHSRS IRILEA+ NLY+A I EK+ MKIGDGSWCP+G+EWTL+TSGH YAVW K
Sbjct:  309 VMEGYAYILTHPGIPSVFYDHFYEWDNSMHDQIVKLIAIRRNQGIHSRSSIRILEAQPNLYAATIDEKVSMKIGDGSWCPAGKEWTLATSGHRYAVWQK 407

[Close]

BLAST of GR395259 vs. TAIR peptide
Match: AT1G76130.1 (| Symbols: ATAMY2, AMY2 | alpha-amylase-like 2 | chr1:28561647-28563914 FORWARD LENGTH=413)

HSP 1 Score: 182.956 bits (463), Expect = 5.385e-47
Identity = 76/99 (76.77%), Postives = 91/99 (91.92%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCPSGREWTLSTSGHNYAVWHK 422
            VMEGYAYILTHPG P +FYDHFYDW +S+H+QIVKLID R+RQ IHSRS +R+L+A+ NLY+AI+GEK+CMK+GDGSWCPSGR+WTL+TSGH YAVWHK
Sbjct:  315 VMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of GR395259 vs. TAIR peptide
Match: AT1G69830.1 (| Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 | chr1:26288518-26293003 REVERSE LENGTH=887)

HSP 1 Score: 96.2857 bits (238), Expect = 6.629e-21
Identity = 43/98 (43.88%), Postives = 66/98 (67.35%), Query Frame = -3

Query:  132 MEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGDGSWCP--SGREWTLSTSGHNYAVW 419
            M+GYAYILTHPGTP++F+DH +   +  H +I  L+  R RQ +H RS + I +++ ++Y+AII EK+ MKIG G + P    + W+++  G +Y VW
Sbjct:  790 MQGYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVW 884

[Close]

BLAST of GR395259 vs. TAIR peptide
Match: AT4G25000.1 (| Symbols: ATAMY1, AMY1 | alpha-amylase-like | chr4:12852109-12853825 REVERSE LENGTH=423)

HSP 1 Score: 85.1149 bits (209), Expect = 1.528e-17
Identity = 47/103 (45.63%), Postives = 65/103 (63.11%), Query Frame = -3

Query:  126 VMEGYAYILTHPGTPSIFYDHFYDWNNSVHEQIVKLIDTRKRQGIHSRSPIRILEAKHNLYSAIIGEKLCMKIGD----GSWCPSGREWTLSTSGHNYAVWHK 422
            V+ GY YILTHPGTP IFY+H+ +W   + E I KL+  R + GI S S + I  A+ +LY A+I +K+ MKIG     G+  PS   + L+ SG ++AVW K
Sbjct:  324 VLLGYVYILTHPGTPCIFYNHYIEW--GLKESISKLVAIRNKNGIGSTSSVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPS--NFALAYSGLDFAVWEK 422

The following BLAST results are available for this feature:

BLAST of GR395259 vs. SwissProt
BLAST of GR395259 vs. TrEMBL
BLAST of GR395259 vs. TAIR peptide

BLAST of GR395259 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AMYA_VIGMU	4.823e-21	51.49	Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1	[more]
AMY2_HORVU	6.298e-21	48.54	Alpha-amylase type B isozyme OS=Hordeum vulgare GN...	[more]
AMY6_HORVU	1.833e-20	49.51	Alpha-amylase type B isozyme OS=Hordeum vulgare GN...	[more]
AMY4_HORVU	1.188e-19	48.54	Alpha-amylase type B isozyme (Fragment) OS=Hordeum...	[more]
AMY1_ORYSJ	2.646e-19	48.54	Alpha-amylase OS=Oryza sativa subsp. japonica GN=A...	[more]
AMY5_HORVU	1.313e-18	47.57	Alpha-amylase type B isozyme (Fragment) OS=Hordeum...	[more]
AMY3_WHEAT	1.715e-18	47.12	Alpha-amylase AMY3 OS=Triticum aestivum GN=AMY1.1 ...	[more]
AMY3C_ORYSJ	2.926e-18	46.60	Alpha-amylase isozyme 3C OS=Oryza sativa subsp. ja...	[more]
AMY3E_ORYSJ	6.518e-18	44.66	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. ja...	[more]
AMY3A_ORYSJ	6.518e-18	44.66	Alpha-amylase isozyme 3A OS=Oryza sativa subsp. ja...	[more]

BLAST of GR395259 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TCX9_SOYBN	4.207e-47	82.83	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q9SGS0_ARATH	8.772e-45	76.77	T23E18.6 OS=Arabidopsis thaliana PE=3 SV=1	[more]
Q8LFG1_ARATH	8.772e-45	76.77	Alpha-amylase like protein OS=Arabidopsis thaliana...	[more]
B9R8H8_RICCO	8.772e-45	82.83	Alpha-amylase, putative OS=Ricinus communis GN=RCO...	[more]
B9GQ54_POPTR	8.772e-45	79.80	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7KTA0_ARALY	3.333e-44	76.77	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q5BLY0_MALDO	1.267e-43	78.79	Cytosolic alpha-amylase OS=Malus domestica GN=AMY9...	[more]
Q9M6R9_MALDO	1.654e-43	77.78	Alpha-amylase OS=Malus domestica PE=2 SV=1	[more]
Q5ZFR9_PLAMJ	4.075e-42	75.76	Alpha-amylase OS=Plantago major GN=aamy1 PE=2 SV=1	[more]
D1MEA8_SOLTU	1.186e-41	76.77	Alpha-amylase OS=Solanum tuberosum GN=amyA2 PE=2 S...	[more]

BLAST of GR395259 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G76130.1	5.385e-47	76.77	\| Symbols: ATAMY2, AMY2 \| alpha-amylase-like 2 \| c...	[more]
AT1G69830.1	6.629e-21	43.88	\| Symbols: ATAMY3, AMY3 \| alpha-amylase-like 3 \| c...	[more]
AT4G25000.1	1.528e-17	45.63	\| Symbols: ATAMY1, AMY1 \| alpha-amylase-like \| chr...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 42..100 score: 3.1
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	no description	coord: 42..100 score: 6.2
IPR013780	Glycosyl hydrolase, family 13, all-beta	GENE3D	2.60.40.1180	no description	coord: 46..100 score: 2.5
IPR017853	Glycoside hydrolase, catalytic core	SUPERFAMILY	51445	(Trans)glycosidases	coord: 6..43 score: 8.9
None	No IPR available	PANTHER	PTHR10357	AMYLASE	coord: 2..77 score: 2
None	No IPR available	PANTHER	PTHR10357:SF9	ALPHA-AMYLASE	coord: 2..77 score: 2
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 46..100 score: 1.8

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR395259 ID=GR395259; Name=GR395259; organism=Cicer arietinum; type=EST; length=427bp
GAAAAAGAGCTGCAGTTTCCTGAAGTATTAGAATTAAAAGCATACTAGTA
TATAAGAAGCACATAATTCCTTTTCATTTCAGTACACACCCAGAGGAAAG
GAATGGTCAAGCTGCAAAAACACTACTTATGCCATACAGCATAATTGTGG
CCACTGGTTGATAGTGTCCATTCCCTCCCAGATGGGCACCATGAACCATC
TCCAATCTTCATGCAGAGTTTCTCCCCGATGATTGCTGAATAAAGGTTGT
GCTTTGCCTCCAGAATCCTAATAGGTGATCTGCTATGAATACCTTGACGC
TTGCGAGTGTCAATCAACTTCACAATCTGCTCATGGACTGAGTTGTTCCA
GTCATAAAAGTGATCATAGAAAATTGACGGGGTCCCAGGATGAGTCAATA
TGTATGCATACCCCTCCATAACCCCGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004556	alpha-amylase activity
GO:0005509	calcium ion binding
GO:0003824	catalytic activity
GO:0043169	cation binding

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR012850	A-amylase_bs_C
IPR013780	Glyco_hydro_b
IPR017853	Glycoside_hydrolase_SF

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