GR399660

EST Overview


Name	GR399660
Unique Name	GR399660
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	521

Libraries


Library Name	Type
LIBEST_024783 ICC4958 Slow drought stresses root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR399660 vs. SwissProt
Match: ATG8I_ARATH (Autophagy-related protein 8i OS=Arabidopsis thaliana GN=ATG8I PE=2 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.755e-26
Identity = 57/91 (62.64%), Postives = 70/91 (76.92%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P +   + E+Y K D P + KKKF VPR++ VG FIYILS+R  L PGKALFVFV NTLPQTA++MD VY+S+KD DGF+Y+ YS EKTFG
Sbjct:   25 PTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIYILSARLHLSPGKALFVFVNNTLPQTAALMDSVYESYKDDDGFVYMCYSSEKTFG 115

[Close]

BLAST of GR399660 vs. SwissProt
Match: ATG8H_ARATH (Autophagy-related protein 8h OS=Arabidopsis thaliana GN=ATG8H PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.177e-22
Identity = 51/91 (56.04%), Postives = 65/91 (71.43%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P++   + E+Y   D P + K K+ VPR++ VG FI++LS R  L P KALFVFV NTLPQTAS MD +Y +FK+ DGFLY+ YS EKTFG
Sbjct:   29 PDRIPVIIEKYSNADLPDMEKNKYLVPRDMTVGHFIHMLSKRMQLDPSKALFVFVHNTLPQTASRMDSLYNTFKEEDGFLYMCYSTEKTFG 119

[Close]

BLAST of GR399660 vs. SwissProt
Match: ATG8D_ORYSJ (Autophagy-related protein 8D OS=Oryza sativa subsp. japonica GN=ATG8D PE=3 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 2.454e-20
Identity = 50/92 (54.35%), Postives = 65/92 (70.65%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKD-ADGFLYLYYSPEKTFG 514
            P++   + E++ + + P + K+K+ VP  + VG FI+IL SR  L PG ALFVFV NTLPQTA +M  VY+S+KD  DGFLYL YS EKTFG
Sbjct:   27 PDRIPVIVEKFSRSNLPEMEKRKYLVPCNMPVGQFIFILRSRLHLSPGTALFVFVNNTLPQTAQLMGSVYESYKDEGDGFLYLCYSSEKTFG 118

[Close]

BLAST of GR399660 vs. SwissProt
Match: ATG8A_ORYSJ (Autophagy-related protein 8A OS=Oryza sativa subsp. japonica GN=ATG8A PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 5.467e-20
Identity = 47/84 (55.95%), Postives = 59/84 (70.24%), Query Frame = -2

Query:  239 ERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGS 490
            E+  K D P + KKK+ VP +L VG F+Y++  R  L P KA+FVFVKNTLP TAS+M  +Y+  KD DGFLY+ YS E TFGS
Sbjct:   35 EKADKTDVPEIDKKKYLVPADLTVGQFVYVVRKRIKLSPEKAIFVFVKNTLPPTASLMSAIYEENKDEDGFLYMTYSGENTFGS 118

[Close]

BLAST of GR399660 vs. SwissProt
Match: ATG8A_ORYSI (Autophagy-related protein 8A OS=Oryza sativa subsp. indica GN=ATG8A PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 5.467e-20
Identity = 47/84 (55.95%), Postives = 59/84 (70.24%), Query Frame = -2

Query:  239 ERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGS 490
            E+  K D P + KKK+ VP +L VG F+Y++  R  L P KA+FVFVKNTLP TAS+M  +Y+  KD DGFLY+ YS E TFGS
Sbjct:   35 EKADKTDVPEIDKKKYLVPADLTVGQFVYVVRKRIKLSPEKAIFVFVKNTLPPTASLMSAIYEENKDEDGFLYMTYSGENTFGS 118

[Close]

BLAST of GR399660 vs. SwissProt
Match: ATG8_VANPO (Autophagy-related protein 8 OS=Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) GN=ATG8 PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 7.141e-20
Identity = 48/97 (49.48%), Postives = 64/97 (65.98%), Query Frame = -2

Query:  239 IPEKFL----CLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGS 517
            I EKF      + E+  K D P + K+K+ VP +L VG F+Y++  R  LPP KA+F+FV +TLP TA++M  VY+  KD DGFLY+ YS E TFGS
Sbjct:   21 ITEKFQNRIPVICEKAEKSDIPEIDKRKYLVPSDLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAVYQEHKDKDGFLYVTYSGENTFGS 117

[Close]

BLAST of GR399660 vs. SwissProt
Match: ATG8_CANGA (Autophagy-related protein 8 OS=Candida glabrata GN=ATG8 PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.591e-19
Identity = 48/96 (50.00%), Postives = 63/96 (65.62%), Query Frame = -2

Query:  242 IPEKFL----CLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 517
            I EKF      + E+  K D P + K+K+ VP +L VG F+Y++  R  LPP KA+F+FV +TLP TAS+M  VY+  KD DGFLY+ YS E TFG
Sbjct:   21 ISEKFQNRIPVICEKAEKSDIPEVDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTASLMSQVYQEHKDKDGFLYVTYSGENTFG 116

[Close]

BLAST of GR399660 vs. SwissProt
Match: ATG8A_ARATH (Autophagy-related protein 8a OS=Arabidopsis thaliana GN=ATG8A PE=1 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 2.078e-19
Identity = 46/94 (48.94%), Postives = 63/94 (67.02%), Query Frame = -2

Query:  233 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGSCT 514
            P++   + E+  + D P + KKK+ VP +L VG F+Y++  R  L   KA+FVFVKNTLP TA++M  +Y+  KD DGFLY+ YS E TFGS T
Sbjct:   27 PDRIPVIVEKAGQSDVPDIDKKKYLVPADLTVGQFVYVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGSLT 120

[Close]

BLAST of GR399660 vs. SwissProt
Match: ATG8_YEAST (Autophagy-related protein 8 OS=Saccharomyces cerevisiae GN=ATG8 PE=1 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 3.544e-19
Identity = 42/83 (50.60%), Postives = 58/83 (69.88%), Query Frame = -2

Query:  242 ERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 490
            E+  K D P + K+K+ VP +L VG F+Y++  R  LPP KA+F+FV +TLP TA++M  +Y+  KD DGFLY+ YS E TFG
Sbjct:   34 EKAEKSDIPEIDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHKDKDGFLYVTYSGENTFG 116

[Close]

BLAST of GR399660 vs. SwissProt
Match: ATG8_YEAS7 (Autophagy-related protein 8 OS=Saccharomyces cerevisiae (strain YJM789) GN=ATG8 PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 3.544e-19
Identity = 42/83 (50.60%), Postives = 58/83 (69.88%), Query Frame = -2

Query:  242 ERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 490
            E+  K D P + K+K+ VP +L VG F+Y++  R  LPP KA+F+FV +TLP TA++M  +Y+  KD DGFLY+ YS E TFG
Sbjct:   34 EKAEKSDIPEIDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHKDKDGFLYVTYSGENTFG 116

[Close]

BLAST of GR399660 vs. TrEMBL
Match: C6T418_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 3.046e-30
Identity = 66/92 (71.74%), Postives = 75/92 (81.52%), Query Frame = -2

Query:  239 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGS 514
            P++   + ERY K D P L KKK+ VPR+L VG FI+ILSSR  LPPGKALFVFVKNTLPQTA+MMD VY+SFKD DGFLY+YYS EKTFGS
Sbjct:   28 PDRVPVIVERYAKCDLPELEKKKYLVPRDLSVGHFIHILSSRLSLPPGKALFVFVKNTLPQTANMMDSVYRSFKDEDGFLYMYYSTEKTFGS 119

[Close]

BLAST of GR399660 vs. TrEMBL
Match: B9A7M1_SOYBN (GmATG8i protein OS=Glycine max GN=GmATG8i PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 3.046e-30
Identity = 66/92 (71.74%), Postives = 75/92 (81.52%), Query Frame = -2

Query:  239 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGS 514
            P++   + ERY K D P L KKK+ VPR+L VG FI+ILSSR  LPPGKALFVFVKNTLPQTA+MMD VY+SFKD DGFLY+YYS EKTFGS
Sbjct:   28 PDRVPVIVERYAKCDLPELEKKKYLVPRDLSVGHFIHILSSRLSLPPGKALFVFVKNTLPQTANMMDSVYRSFKDEDGFLYMYYSTEKTFGS 119

[Close]

BLAST of GR399660 vs. TrEMBL
Match: D7U2P9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_5.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00028202001 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.486e-26
Identity = 60/91 (65.93%), Postives = 71/91 (78.02%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P++   + ERY K D P + KKKF VPR++ VG FI+ILS R  L PGKALFVFV+NTLPQTAS+MD VY+SFKD DGFLY+ YS EKTFG
Sbjct:   25 PDRVPVIAERYSKTDLPEMEKKKFLVPRDMSVGQFIHILSIRLHLTPGKALFVFVENTLPQTASLMDTVYESFKDEDGFLYMSYSSEKTFG 115

[Close]

BLAST of GR399660 vs. TrEMBL
Match: B9RN69_RICCO (Autophagy 8h, putative OS=Ricinus communis GN=RCOM_1344100 PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.486e-26
Identity = 58/94 (61.70%), Postives = 73/94 (77.66%), Query Frame = -2

Query:  233 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGSCT 514
            P++   + ERY K D P L KKK+ VPR++ VG FI++LSSR  + PGKALFVFV+NTLPQTA++M+ VY+SFKD DGFLY+ YS EKTFG  T
Sbjct:   28 PDRVPVIVERYCKTDLPELEKKKYLVPRDMSVGQFIHVLSSRLRMAPGKALFVFVENTLPQTATLMNSVYESFKDGDGFLYMCYSSEKTFGHAT 121

[Close]

BLAST of GR399660 vs. TrEMBL
Match: A6P6J6_FAGES (Autophagy-related protein 8 OS=Fagopyrum esculentum GN=FeATG8-1 PE=2 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 7.765e-26
Identity = 57/94 (60.64%), Postives = 72/94 (76.60%), Query Frame = -2

Query:  233 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGSCT 514
            P++   + ERY K + P L K+KF VPR++ VG FI+ILS R  LPPGKALF+FV NTLPQT S+M+ +Y+SFKD DGFLY++YS EKTFG  T
Sbjct:   28 PDRVPLIAERYSKTELPELEKRKFLVPRDMSVGQFIHILSGRLHLPPGKALFIFVNNTLPQTTSLMNSLYESFKDEDGFLYMFYSSEKTFGCDT 121

[Close]

BLAST of GR399660 vs. TrEMBL
Match: B9HXH0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_659176 PE=3 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.325e-25
Identity = 57/91 (62.64%), Postives = 71/91 (78.02%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P++   + ERY + D P + K+KF VPR++ +G FI+ILSSR  L PGKALFVFVKNTLPQTAS MD +Y+S+KD DGFLY+ YS EKTFG
Sbjct:   28 PDRVPVIIERYSRTDLPEMEKRKFLVPRDMTIGQFIHILSSRLELTPGKALFVFVKNTLPQTASQMDSIYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of GR399660 vs. TrEMBL
Match: D7L4V3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_479007 PE=4 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.730e-25
Identity = 57/91 (62.64%), Postives = 70/91 (76.92%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P +   + E+Y K D P + KKKF VPR++ VG FIYILS+R  L PGKALFVFV NTLPQTA++MD VY+S+KD DGF+Y+ YS EKTFG
Sbjct:   25 PTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIYILSARLHLSPGKALFVFVNNTLPQTAALMDSVYESYKDEDGFVYMCYSSEKTFG 115

[Close]

BLAST of GR399660 vs. TrEMBL
Match: B9HII0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_719853 PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 3.854e-25
Identity = 55/91 (60.44%), Postives = 71/91 (78.02%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P++   + ERY + D P + K+K+ VPR++ +G FI+ILSSR  L PGKALF+FVKNTLPQTAS MD +Y+S+KD DGFLY+ YS EKTFG
Sbjct:   28 PDRVPVIIERYSRTDLPEMEKRKYLVPRDMTIGQFIHILSSRLELTPGKALFIFVKNTLPQTASQMDSIYESYKDDDGFLYMCYSSEKTFG 118

[Close]

BLAST of GR399660 vs. TrEMBL
Match: B9GSZ0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_816749 PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 3.854e-25
Identity = 59/89 (66.29%), Postives = 69/89 (77.53%), Query Frame = -2

Query:  224 ERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGSCT*IS 490
            ERY K D P + KKK+ VPR++ VG FI+ILSSR  L PGKALFVFVK+TLPQTA++MD VY+S KD DGFLY+ YS EKTFG    IS
Sbjct:   34 ERYCKTDLPEMEKKKYLVPRDMSVGQFIHILSSRLRLTPGKALFVFVKDTLPQTAALMDSVYESLKDEDGFLYMCYSSEKTFGHTVPIS 122

[Close]

BLAST of GR399660 vs. TrEMBL
Match: B9RZY9_RICCO (Autophagy 8h, putative OS=Ricinus communis GN=RCOM_1003180 PE=3 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 6.574e-25
Identity = 58/91 (63.74%), Postives = 68/91 (74.73%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P +   + ERY K D P + K+K+ VPR   VG FI+ILS R  L PGKALFVFVKNTLP TAS+MD VY+S+KD DGFLY+YYS EKTFG
Sbjct:   25 PGRVPVIIERYSKTDLPEMEKRKYLVPRATSVGQFIHILSGRLHLAPGKALFVFVKNTLPPTASLMDSVYESYKDDDGFLYMYYSSEKTFG 115

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT3G15580.1 (| Symbols: ATG8I, APG8H | Ubiquitin-like superfamily protein | chr3:5274075-5275000 REVERSE LENGTH=115)

HSP 1 Score: 118.627 bits (296), Expect = 1.938e-27
Identity = 57/91 (62.64%), Postives = 70/91 (76.92%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P +   + E+Y K D P + KKKF VPR++ VG FIYILS+R  L PGKALFVFV NTLPQTA++MD VY+S+KD DGF+Y+ YS EKTFG
Sbjct:   25 PTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIYILSARLHLSPGKALFVFVNNTLPQTAALMDSVYESYKDDDGFVYMCYSSEKTFG 115

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT3G06420.1 (| Symbols: ATG8H | Ubiquitin-like superfamily protein | chr3:1955219-1956274 REVERSE LENGTH=119)

HSP 1 Score: 105.916 bits (263), Expect = 1.300e-23
Identity = 51/91 (56.04%), Postives = 65/91 (71.43%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P++   + E+Y   D P + K K+ VPR++ VG FI++LS R  L P KALFVFV NTLPQTAS MD +Y +FK+ DGFLY+ YS EKTFG
Sbjct:   29 PDRIPVIIEKYSNADLPDMEKNKYLVPRDMTVGHFIHMLSKRMQLDPSKALFVFVHNTLPQTASRMDSLYNTFKEEDGFLYMCYSTEKTFG 119

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT4G21980.2 (| Symbols: APG8A, ATG8A | Ubiquitin-like superfamily protein | chr4:11655868-11656809 FORWARD LENGTH=137)

HSP 1 Score: 95.1301 bits (235), Expect = 2.295e-20
Identity = 46/94 (48.94%), Postives = 63/94 (67.02%), Query Frame = -2

Query:  233 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGSCT 514
            P++   + E+  + D P + KKK+ VP +L VG F+Y++  R  L   KA+FVFVKNTLP TA++M  +Y+  KD DGFLY+ YS E TFGS T
Sbjct:   42 PDRIPVIVEKAGQSDVPDIDKKKYLVPADLTVGQFVYVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGSLT 135

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT4G21980.1 (| Symbols: APG8A, ATG8A | Ubiquitin-like superfamily protein | chr4:11655913-11656809 FORWARD LENGTH=122)

HSP 1 Score: 95.1301 bits (235), Expect = 2.295e-20
Identity = 46/94 (48.94%), Postives = 63/94 (67.02%), Query Frame = -2

Query:  233 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFGSCT 514
            P++   + E+  + D P + KKK+ VP +L VG F+Y++  R  L   KA+FVFVKNTLP TA++M  +Y+  KD DGFLY+ YS E TFGS T
Sbjct:   27 PDRIPVIVEKAGQSDVPDIDKKKYLVPADLTVGQFVYVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGSLT 120

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT2G05630.2 (| Symbols: ATG8D | Ubiquitin-like superfamily protein | chr2:2083211-2084775 REVERSE LENGTH=164)

HSP 1 Score: 94.3597 bits (233), Expect = 3.914e-20
Identity = 44/91 (48.35%), Postives = 61/91 (67.03%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P++   + ER  K D P + +KK+ VP +L VG F+Y++  R  L P KA+F+FVKN LP TA++M  +Y+  KD DGFLY+ YS E TFG
Sbjct:   71 PDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQFVYVVRKRIKLSPEKAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFG 161

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT2G05630.1 (| Symbols: ATG8D | Ubiquitin-like superfamily protein | chr2:2083211-2084775 REVERSE LENGTH=120)

HSP 1 Score: 94.3597 bits (233), Expect = 3.914e-20
Identity = 44/91 (48.35%), Postives = 61/91 (67.03%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P++   + ER  K D P + +KK+ VP +L VG F+Y++  R  L P KA+F+FVKN LP TA++M  +Y+  KD DGFLY+ YS E TFG
Sbjct:   27 PDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQFVYVVRKRIKLSPEKAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFG 117

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT1G62040.2 (| Symbols: ATG8C | Ubiquitin-like superfamily protein | chr1:22932782-22934253 FORWARD LENGTH=133)

HSP 1 Score: 94.3597 bits (233), Expect = 3.914e-20
Identity = 46/91 (50.55%), Postives = 60/91 (65.93%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P++   + ER  + D P + KKK+ VP +L VG F+Y++  R  L   KA+FVFVKNTLP TA+MM  +Y   KD DGFLY+ YS E TFG
Sbjct:   41 PDRIPVIVERAERSDVPNIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFVFVKNTLPPTAAMMSAIYDENKDEDGFLYMTYSGENTFG 131

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT1G62040.1 (| Symbols: ATG8C | Ubiquitin-like superfamily protein | chr1:22933216-22934253 FORWARD LENGTH=119)

HSP 1 Score: 94.3597 bits (233), Expect = 3.914e-20
Identity = 46/91 (50.55%), Postives = 60/91 (65.93%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            P++   + ER  + D P + KKK+ VP +L VG F+Y++  R  L   KA+FVFVKNTLP TA+MM  +Y   KD DGFLY+ YS E TFG
Sbjct:   27 PDRIPVIVERAERSDVPNIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFVFVKNTLPPTAAMMSAIYDENKDEDGFLYMTYSGENTFG 117

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT4G04620.2 (| Symbols: ATG8B | Ubiquitin-like superfamily protein | chr4:2328663-2329926 REVERSE LENGTH=122)

HSP 1 Score: 92.4337 bits (228), Expect = 1.487e-19
Identity = 44/91 (48.35%), Postives = 61/91 (67.03%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            PE+   + E+  + D P + KKK+ VP +L +G F+Y++  R  L   KA+FVFVKNTLP TA++M  +Y+  KD DGFLY+ YS E TFG
Sbjct:   27 PERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFVYVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFG 117

[Close]

BLAST of GR399660 vs. TAIR peptide
Match: AT4G04620.1 (| Symbols: ATG8B | Ubiquitin-like superfamily protein | chr4:2328663-2329926 REVERSE LENGTH=122)

HSP 1 Score: 92.4337 bits (228), Expect = 1.487e-19
Identity = 44/91 (48.35%), Postives = 61/91 (67.03%), Query Frame = -2

Query:  242 PEKFLCLWERYLK*DFPYLGKKKFFVPRELFVGPFIYILSSRFCLPPGKALFVFVKNTLPQTASMMDFVYKSFKDADGFLYLYYSPEKTFG 514
            PE+   + E+  + D P + KKK+ VP +L +G F+Y++  R  L   KA+FVFVKNTLP TA++M  +Y+  KD DGFLY+ YS E TFG
Sbjct:   27 PERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFVYVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFG 117

The following BLAST results are available for this feature:

BLAST of GR399660 vs. SwissProt
BLAST of GR399660 vs. TrEMBL
BLAST of GR399660 vs. TAIR peptide

BLAST of GR399660 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ATG8I_ARATH	1.755e-26	62.64	Autophagy-related protein 8i OS=Arabidopsis thalia...	[more]
ATG8H_ARATH	1.177e-22	56.04	Autophagy-related protein 8h OS=Arabidopsis thalia...	[more]
ATG8D_ORYSJ	2.454e-20	54.35	Autophagy-related protein 8D OS=Oryza sativa subsp...	[more]
ATG8A_ORYSJ	5.467e-20	55.95	Autophagy-related protein 8A OS=Oryza sativa subsp...	[more]
ATG8A_ORYSI	5.467e-20	55.95	Autophagy-related protein 8A OS=Oryza sativa subsp...	[more]
ATG8_VANPO	7.141e-20	49.48	Autophagy-related protein 8 OS=Vanderwaltozyma pol...	[more]
ATG8_CANGA	1.591e-19	50.00	Autophagy-related protein 8 OS=Candida glabrata GN...	[more]
ATG8A_ARATH	2.078e-19	48.94	Autophagy-related protein 8a OS=Arabidopsis thalia...	[more]
ATG8_YEAST	3.544e-19	50.60	Autophagy-related protein 8 OS=Saccharomyces cerev...	[more]
ATG8_YEAS7	3.544e-19	50.60	Autophagy-related protein 8 OS=Saccharomyces cerev...	[more]

BLAST of GR399660 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T418_SOYBN	3.046e-30	71.74	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9A7M1_SOYBN	3.046e-30	71.74	GmATG8i protein OS=Glycine max GN=GmATG8i PE=2 SV=...	[more]
D7U2P9_VITVI	3.486e-26	65.93	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RN69_RICCO	3.486e-26	61.70	Autophagy 8h, putative OS=Ricinus communis GN=RCOM...	[more]
A6P6J6_FAGES	7.765e-26	60.64	Autophagy-related protein 8 OS=Fagopyrum esculentu...	[more]
B9HXH0_POPTR	1.325e-25	62.64	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7L4V3_ARALY	1.730e-25	62.64	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9HII0_POPTR	3.854e-25	60.44	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9GSZ0_POPTR	3.854e-25	66.29	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RZY9_RICCO	6.574e-25	63.74	Autophagy 8h, putative OS=Ricinus communis GN=RCOM...	[more]

BLAST of GR399660 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G15580.1	1.938e-27	62.64	\| Symbols: ATG8I, APG8H \| Ubiquitin-like superfami...	[more]
AT3G06420.1	1.300e-23	56.04	\| Symbols: ATG8H \| Ubiquitin-like superfamily prot...	[more]
AT4G21980.2	2.295e-20	48.94	\| Symbols: APG8A, ATG8A \| Ubiquitin-like superfami...	[more]
AT4G21980.1	2.295e-20	48.94	\| Symbols: APG8A, ATG8A \| Ubiquitin-like superfami...	[more]
AT2G05630.2	3.914e-20	48.35	\| Symbols: ATG8D \| Ubiquitin-like superfamily prot...	[more]
AT2G05630.1	3.914e-20	48.35	\| Symbols: ATG8D \| Ubiquitin-like superfamily prot...	[more]
AT1G62040.2	3.914e-20	50.55	\| Symbols: ATG8C \| Ubiquitin-like superfamily prot...	[more]
AT1G62040.1	3.914e-20	50.55	\| Symbols: ATG8C \| Ubiquitin-like superfamily prot...	[more]
AT4G04620.2	1.487e-19	48.35	\| Symbols: ATG8B \| Ubiquitin-like superfamily prot...	[more]
AT4G04620.1	1.487e-19	48.35	\| Symbols: ATG8B \| Ubiquitin-like superfamily prot...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004241	Light chain 3 (LC3)	PANTHER	PTHR10969	MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED	coord: 1..77 score: 2.1
IPR004241	Light chain 3 (LC3)	PFAM	PF02991	MAP1_LC3	coord: 2..77 score: 3.4
None	No IPR available	GENE3D	3.10.20.90	no description	coord: 3..77 score: 1.4
None	No IPR available	PANTHER	PTHR10969:SF3	AUTOPHAGY 8H (SYMBIOSIS-RELATED LIKE PROTEIN)	coord: 1..77 score: 2.1
None	No IPR available	SUPERFAMILY	54236	Ubiquitin-like	coord: 1..77 score: 8.7

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR399660 ID=GR399660; Name=GR399660; organism=Cicer arietinum; type=EST; length=521bp
CTAAACATAGGAATTTTTCACGAGTAAAAATTGAATTCACTATTCCAGGG
TTTACAACATTCACACAAACACACAAACCACTTGCGCTAGACCTCGGGGG
TATGTTATACATAGCATTTGAAAGGGGAAAAAGAACACAATAATTAGTAA
AACCATACAACAAATATGTACAGGTTTTTTACATAAAACGGCTCATAACA
AGTGTATAAAAAAGTTTAATAGTTGATATTTATGTACAGGATCCAAAGGT
TTTCTCAGGGCTATAATACAGGTAAAGAAATCCATCCGCATCTTTGAATG
ATTTATAAACAAAATCCATCATACTAGCAGTTTGAGGTAAGGTATTCTTC
ACAAAAACAAAAAGGGCTTTTCCAGGGGGTAAACAAAATCTTGAACTCAA
AATATAAATGAAAGGGCCAACAAACAATTCTCGGGGAACAAAAAATTTTT
TCTTCCCCAAATAAGGAAAATCCTATTTCAAATACCTTTCCCACAAACAC
AGAAATTTTTCTGGAATAATT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR004241	Atg8-like

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