DY475457

EST Overview


Name	DY475457
Unique Name	DY475457
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	255

Libraries


Library Name	Type
LIBEST_016683 Chickpea (ICC3996) post-inoculation EST library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of DY475457 vs. SwissProt
Match: DLDH_SYNY3 (Dihydrolipoyl dehydrogenase OS=Synechocystis sp. (strain PCC 6803) GN=lpdA PE=1 SV=3)

HSP 1 Score: 57.7658 bits (138), Expect = 9.099e-12
Identity = 31/54 (57.41%), Postives = 43/54 (79.63%), Query Frame = 3

Query:   93 LEVDAALIATGRVPFTQGLGLENVDVAT-QRGFVPVDERMRVIDANGKLVPNLY 251
            LEVDA L+ATGR+P T+ LGLE V V T +RGF+ V+++M+VI  +GK VP+L+
Sbjct:  267 LEVDACLVATGRIPATKNLGLETVGVETDRRGFIEVNDQMQVI-KDGKPVPHLW 319

HSP 2 Score: 31.187 bits (69), Expect = 9.099e-12
Identity = 19/31 (61.29%), Postives = 21/31 (67.74%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFA+KI   + G PV IEL DA TKE  DT
Sbjct:  239 GVFATKI---KAGSPVEIELTDAKTKEVIDT 266

[Close]

BLAST of DY475457 vs. TrEMBL
Match: D5MAF8_TRIRP (Dihydrolipoyl dehydrogenase OS=Trifolium repens GN=LPD1 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 2.077e-33
Identity = 51/57 (89.47%), Postives = 54/57 (94.74%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT+GLGLENVDVATQRGF+PVDERMRVIDANGKLVP+LYC
Sbjct:  348 KDTLEVDAALIATGRAPFTEGLGLENVDVATQRGFIPVDERMRVIDANGKLVPHLYC 404

HSP 2 Score: 60.077 bits (144), Expect = 2.077e-33
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP+RDGKPVLIELIDA TKEPKDT
Sbjct:  320 GVFASKITPARDGKPVLIELIDAKTKEPKDT 350

[Close]

BLAST of DY475457 vs. TrEMBL
Match: D5MAF7_TRIRP (Dihydrolipoyl dehydrogenase OS=Trifolium repens GN=LPD2-accD PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.088e-31
Identity = 51/57 (89.47%), Postives = 53/57 (92.98%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFTQGLGLEN+DVATQRGFVPVDERMRVIDANG LVP+LYC
Sbjct:  342 KDTLEVDAALIATGRAPFTQGLGLENIDVATQRGFVPVDERMRVIDANGNLVPHLYC 398

HSP 2 Score: 55.0694 bits (131), Expect = 1.088e-31
Identity = 27/31 (87.10%), Postives = 29/31 (93.55%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP+R+GKPVLIELIDA TKE KDT
Sbjct:  314 GVFASKITPARNGKPVLIELIDAKTKEQKDT 344

[Close]

BLAST of DY475457 vs. TrEMBL
Match: Q2HU25_MEDTR (Dihydrolipoyl dehydrogenase OS=Medicago truncatula GN=MtrDRAFT_AC149491g1v2 PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 1.854e-31
Identity = 51/57 (89.47%), Postives = 53/57 (92.98%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFTQGLGLEN+DVATQRGFVPVDERMRVIDANG LVP+LYC
Sbjct:  343 KETLEVDAALIATGRAPFTQGLGLENIDVATQRGFVPVDERMRVIDANGNLVPHLYC 399

HSP 2 Score: 54.6842 bits (130), Expect = 1.854e-31
Identity = 26/31 (83.87%), Postives = 29/31 (93.55%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP+RDGKPV+IELIDA TKE K+T
Sbjct:  315 GVFASKITPARDGKPVMIELIDAKTKEQKET 345

[Close]

BLAST of DY475457 vs. TrEMBL
Match: Q9M5K4_ARATH (Dihydrolipoyl dehydrogenase OS=Arabidopsis thaliana GN=lpd2 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 3.144e-31
Identity = 47/57 (82.46%), Postives = 51/57 (89.47%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLEN++V TQRGF+PVDERMRVID NGKLVP+LYC
Sbjct:  341 KDTLEVDAALIATGRAPFTNGLGLENINVTTQRGFIPVDERMRVIDGNGKLVPHLYC 397

HSP 2 Score: 58.9214 bits (141), Expect = 3.144e-31
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP++DGKPVLIELIDA TKEPKDT
Sbjct:  313 GVFASKITPAKDGKPVLIELIDAKTKEPKDT 343

[Close]

BLAST of DY475457 vs. TrEMBL
Match: B9DG34_ARATH (Dihydrolipoyl dehydrogenase OS=Arabidopsis thaliana GN=AT3G16950 PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 6.934e-31
Identity = 47/57 (82.46%), Postives = 49/57 (85.96%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLENV+V TQRGF+PVDERMRVID  G LVPNLYC
Sbjct:  344 KDTLEVDAALIATGRAPFTNGLGLENVNVVTQRGFIPVDERMRVIDGKGTLVPNLYC 400

HSP 2 Score: 60.077 bits (144), Expect = 6.934e-31
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP+RDGKPVLIELIDA TKEPKDT
Sbjct:  316 GVFASKITPARDGKPVLIELIDAKTKEPKDT 346

[Close]

BLAST of DY475457 vs. TrEMBL
Match: A8MS68_ARATH (Dihydrolipoyl dehydrogenase OS=Arabidopsis thaliana GN=At3g16950 PE=3 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 6.934e-31
Identity = 47/57 (82.46%), Postives = 49/57 (85.96%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLENV+V TQRGF+PVDERMRVID  G LVPNLYC
Sbjct:  344 KDTLEVDAALIATGRAPFTNGLGLENVNVVTQRGFIPVDERMRVIDGKGTLVPNLYC 400

HSP 2 Score: 60.077 bits (144), Expect = 6.934e-31
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP+RDGKPVLIELIDA TKEPKDT
Sbjct:  316 GVFASKITPARDGKPVLIELIDAKTKEPKDT 346

[Close]

BLAST of DY475457 vs. TrEMBL
Match: D7L694_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_479163 PE=4 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 6.935e-31
Identity = 48/57 (84.21%), Postives = 50/57 (87.72%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLENV+V TQRGF+PVDERMRVID NG LVPNLYC
Sbjct:  340 KDTLEVDAALIATGRAPFTNGLGLENVNVVTQRGFIPVDERMRVIDGNGTLVPNLYC 396

HSP 2 Score: 57.7658 bits (138), Expect = 6.935e-31
Identity = 27/31 (87.10%), Postives = 30/31 (96.77%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFA+KITP++DGKPVLIELIDA TKEPKDT
Sbjct:  312 GVFATKITPAKDGKPVLIELIDAKTKEPKDT 342

[Close]

BLAST of DY475457 vs. TrEMBL
Match: Q9M5K5_ARATH (Dihydrolipoyl dehydrogenase OS=Arabidopsis thaliana GN=lpd1 PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 6.942e-31
Identity = 47/57 (82.46%), Postives = 49/57 (85.96%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLENV+V TQRGF+PVDERMRVID  G LVPNLYC
Sbjct:  344 KDTLEVDAALIATGRAPFTNGLGLENVNVVTQRGFIPVDERMRVIDGKGTLVPNLYC 400

HSP 2 Score: 60.077 bits (144), Expect = 6.942e-31
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP+RDGKPVLIELIDA TKEPKDT
Sbjct:  316 GVFASKITPARDGKPVLIELIDAKTKEPKDT 346

[Close]

BLAST of DY475457 vs. TrEMBL
Match: D7MA71_ARALY (Plastidic dihydrolipoamide dehydrogenase 2 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_915150 PE=4 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 4.408e-30
Identity = 46/57 (80.70%), Postives = 51/57 (89.47%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLEN++V TQRGF+PVDERMRVID NGKL+P+LYC
Sbjct:  342 KDTLEVDAALIATGRAPFTNGLGLENINVVTQRGFIPVDERMRVIDGNGKLIPHLYC 398

HSP 2 Score: 55.4546 bits (132), Expect = 4.408e-30
Identity = 26/31 (83.87%), Postives = 29/31 (93.55%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP++ GKPV+IELIDA TKEPKDT
Sbjct:  314 GVFASKITPAKGGKPVMIELIDAKTKEPKDT 344

[Close]

BLAST of DY475457 vs. TrEMBL
Match: D7SY46_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_77.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035022001 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 3.642e-29
Identity = 46/57 (80.70%), Postives = 50/57 (87.72%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLEN+ V TQRGF+PVDERMRVIDA+G LVP+LYC
Sbjct:  346 KDALEVDAALIATGRAPFTNGLGLENISVVTQRGFIPVDERMRVIDADGNLVPHLYC 402

HSP 2 Score: 54.6842 bits (130), Expect = 3.642e-29
Identity = 26/30 (86.67%), Postives = 28/30 (93.33%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKD 90
            GVFASKITP++DGKPV IELIDA TKEPKD
Sbjct:  318 GVFASKITPAKDGKPVRIELIDAKTKEPKD 347

[Close]

BLAST of DY475457 vs. TAIR peptide
Match: AT4G16155.1 (| Symbols: | dihydrolipoyl dehydrogenases | chr4:9153570-9157322 REVERSE LENGTH=630)

HSP 1 Score: 100.138 bits (248), Expect = 1.221e-33
Identity = 47/57 (82.46%), Postives = 51/57 (89.47%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLEN++V TQRGF+PVDERMRVID NGKLVP+LYC
Sbjct:  404 KDTLEVDAALIATGRAPFTNGLGLENINVTTQRGFIPVDERMRVIDGNGKLVPHLYC 460

HSP 2 Score: 58.9214 bits (141), Expect = 1.221e-33
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP++DGKPVLIELIDA TKEPKDT
Sbjct:  376 GVFASKITPAKDGKPVLIELIDAKTKEPKDT 406

[Close]

BLAST of DY475457 vs. TAIR peptide
Match: AT3G16950.2 (| Symbols: LPD1, ptlpd1 | lipoamide dehydrogenase 1 | chr3:5786508-5790383 REVERSE LENGTH=623)

HSP 1 Score: 97.8265 bits (242), Expect = 2.696e-33
Identity = 47/57 (82.46%), Postives = 49/57 (85.96%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLENV+V TQRGF+PVDERMRVID  G LVPNLYC
Sbjct:  344 KDTLEVDAALIATGRAPFTNGLGLENVNVVTQRGFIPVDERMRVIDGKGTLVPNLYC 400

HSP 2 Score: 60.077 bits (144), Expect = 2.696e-33
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP+RDGKPVLIELIDA TKEPKDT
Sbjct:  316 GVFASKITPARDGKPVLIELIDAKTKEPKDT 346

[Close]

BLAST of DY475457 vs. TAIR peptide
Match: AT3G16950.1 (| Symbols: LPD1, ptlpd1 | lipoamide dehydrogenase 1 | chr3:5786761-5790383 REVERSE LENGTH=570)

HSP 1 Score: 97.8265 bits (242), Expect = 2.699e-33
Identity = 47/57 (82.46%), Postives = 49/57 (85.96%), Query Frame = 3

Query:   84 KGHLEVDAALIATGRVPFTQGLGLENVDVATQRGFVPVDERMRVIDANGKLVPNLYC 254
            K  LEVDAALIATGR PFT GLGLENV+V TQRGF+PVDERMRVID  G LVPNLYC
Sbjct:  344 KDTLEVDAALIATGRAPFTNGLGLENVNVVTQRGFIPVDERMRVIDGKGTLVPNLYC 400

HSP 2 Score: 60.077 bits (144), Expect = 2.699e-33
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 1

Query:    1 GVFASKITPSRDGKPVLIELIDA*TKEPKDT 93
            GVFASKITP+RDGKPVLIELIDA TKEPKDT
Sbjct:  316 GVFASKITPARDGKPVLIELIDAKTKEPKDT 346

The following BLAST results are available for this feature:

BLAST of DY475457 vs. SwissProt
BLAST of DY475457 vs. TrEMBL
BLAST of DY475457 vs. TAIR peptide

BLAST of DY475457 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
DLDH_SYNY3	9.099e-12	57.41	Dihydrolipoyl dehydrogenase OS=Synechocystis sp. (...	[more]

BLAST of DY475457 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D5MAF8_TRIRP	2.077e-33	89.47	Dihydrolipoyl dehydrogenase OS=Trifolium repens GN...	[more]
D5MAF7_TRIRP	1.088e-31	89.47	Dihydrolipoyl dehydrogenase OS=Trifolium repens GN...	[more]
Q2HU25_MEDTR	1.854e-31	89.47	Dihydrolipoyl dehydrogenase OS=Medicago truncatula...	[more]
Q9M5K4_ARATH	3.144e-31	82.46	Dihydrolipoyl dehydrogenase OS=Arabidopsis thalian...	[more]
B9DG34_ARATH	6.934e-31	82.46	Dihydrolipoyl dehydrogenase OS=Arabidopsis thalian...	[more]
A8MS68_ARATH	6.934e-31	82.46	Dihydrolipoyl dehydrogenase OS=Arabidopsis thalian...	[more]
D7L694_ARALY	6.935e-31	84.21	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9M5K5_ARATH	6.942e-31	82.46	Dihydrolipoyl dehydrogenase OS=Arabidopsis thalian...	[more]
D7MA71_ARALY	4.408e-30	80.70	Plastidic dihydrolipoamide dehydrogenase 2 OS=Arab...	[more]
D7SY46_VITVI	3.642e-29	80.70	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of DY475457 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G16155.1	1.221e-33	82.46	\| Symbols: \| dihydrolipoyl dehydrogenases \| chr4:...	[more]
AT3G16950.2	2.696e-33	82.46	\| Symbols: LPD1, ptlpd1 \| lipoamide dehydrogenase ...	[more]
AT3G16950.1	2.699e-33	82.46	\| Symbols: LPD1, ptlpd1 \| lipoamide dehydrogenase ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006258	Dihydrolipoamide dehydrogenase	PANTHER	PTHR22912:SF20	DIHYDROLIPOAMIDE DEHYDROGENASE-RELATED	coord: 9..63 score: 7
None	No IPR available	GENE3D	3.50.50.60	no description	coord: 8..63 score: 6.4
None	No IPR available	PANTHER	PTHR22912	DISULFIDE OXIDOREDUCTASE	coord: 9..63 score: 7

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DY475457 ID=DY475457; Name=DY475457; organism=Cicer arietinum; type=EST; length=255bp
GGAGTTTTTGCATCCAAGATCACTCCTTCAAGGGATGGAAAACCTGTCTT
GATTGAACTTATTGATGCATAAACCAAGGAACCAAAGGACACTTGGAGGT
GGATGCTGCACTAATAGCAACTGGAAGGGTTCCATTCACACAAGGTCTAG
GACTGGAGAATGTTGATGTAGCAACACAGCGAGGCTTTGTTCCTGTGGAC
GAGCGCATGCGTGTAATTGATGCAAATGGCAAGCTGGTACCTAATTTATA
CTGTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004148	dihydrolipoyl dehydrogenase activity
GO:0050660	flavin adenine dinucleotide binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR006258	Lipoamide_DH

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