GR915685

EST Overview
NameGR915685
Unique NameGR915685
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length205
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_YEAST (2-oxoglutarate dehydrogenase, mitochondrial OS=Saccharomyces cerevisiae GN=KGD1 PE=1 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 3.286e-14
Identity = 36/60 (60.00%), Postives = 46/60 (76.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            S+ PE F  HK +KRI + R + IETGE IDW  GEALAF TL+++G +VR+SG+DVERG
Sbjct:  617 SSWPEGFEVHKNLKRILKNRGKSIETGEGIDWATGEALAFGTLVLDGQNVRVSGEDVERG 676
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_BRUSU (2-oxoglutarate dehydrogenase E1 component OS=Brucella suis GN=sucA PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.782e-13
Identity = 32/58 (55.17%), Postives = 45/58 (77.59%), Query Frame = 2 
Query:   32 IPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +P++F  H+ ++R  + RA+M+ETGE IDW   E+LAF +L VEG+ +RLSGQDVERG
Sbjct:  624 VPKDFHVHRTIQRFLDNRAKMMETGEGIDWATAESLAFGSLAVEGHPIRLSGQDVERG 681
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_BRUSI (2-oxoglutarate dehydrogenase E1 component OS=Brucella suis (strain ATCC 23445 / NCTC 10510) GN=sucA PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.782e-13
Identity = 32/58 (55.17%), Postives = 45/58 (77.59%), Query Frame = 2 
Query:   32 IPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +P++F  H+ ++R  + RA+M+ETGE IDW   E+LAF +L VEG+ +RLSGQDVERG
Sbjct:  624 VPKDFHVHRTIQRFLDNRAKMMETGEGIDWATAESLAFGSLAVEGHPIRLSGQDVERG 681
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_BRUO2 (2-oxoglutarate dehydrogenase E1 component OS=Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) GN=sucA PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.782e-13
Identity = 32/58 (55.17%), Postives = 45/58 (77.59%), Query Frame = 2 
Query:   32 IPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +P++F  H+ ++R  + RA+M+ETGE IDW   E+LAF +L VEG+ +RLSGQDVERG
Sbjct:  624 VPKDFHVHRTIQRFLDNRAKMMETGEGIDWATAESLAFGSLAVEGHPIRLSGQDVERG 681
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_BRUC2 (2-oxoglutarate dehydrogenase E1 component OS=Brucella canis (strain ATCC 23365 / NCTC 10854) GN=sucA PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.782e-13
Identity = 32/58 (55.17%), Postives = 45/58 (77.59%), Query Frame = 2 
Query:   32 IPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +P++F  H+ ++R  + RA+M+ETGE IDW   E+LAF +L VEG+ +RLSGQDVERG
Sbjct:  624 VPKDFHVHRTIQRFLDNRAKMMETGEGIDWATAESLAFGSLAVEGHPIRLSGQDVERG 681
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_BRUAB (2-oxoglutarate dehydrogenase E1 component OS=Brucella abortus GN=sucA PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.782e-13
Identity = 32/58 (55.17%), Postives = 45/58 (77.59%), Query Frame = 2 
Query:   32 IPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +P++F  H+ ++R  + RA+M+ETGE IDW   E+LAF +L VEG+ +RLSGQDVERG
Sbjct:  624 VPKDFHVHRTIQRFLDNRAKMMETGEGIDWATAESLAFGSLAVEGHPIRLSGQDVERG 681
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_BRUA2 (2-oxoglutarate dehydrogenase E1 component OS=Brucella abortus (strain 2308) GN=sucA PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.782e-13
Identity = 32/58 (55.17%), Postives = 45/58 (77.59%), Query Frame = 2 
Query:   32 IPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +P++F  H+ ++R  + RA+M+ETGE IDW   E+LAF +L VEG+ +RLSGQDVERG
Sbjct:  624 VPKDFHVHRTIQRFLDNRAKMMETGEGIDWATAESLAFGSLAVEGHPIRLSGQDVERG 681
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_BRUA1 (2-oxoglutarate dehydrogenase E1 component OS=Brucella abortus (strain S19) GN=sucA PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.782e-13
Identity = 32/58 (55.17%), Postives = 45/58 (77.59%), Query Frame = 2 
Query:   32 IPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +P++F  H+ ++R  + RA+M+ETGE IDW   E+LAF +L VEG+ +RLSGQDVERG
Sbjct:  624 VPKDFHVHRTIQRFLDNRAKMMETGEGIDWATAESLAFGSLAVEGHPIRLSGQDVERG 681
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_OCHA4 (2-oxoglutarate dehydrogenase E1 component OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) GN=sucA PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.745e-13
Identity = 31/58 (53.45%), Postives = 45/58 (77.59%), Query Frame = 2 
Query:   32 IPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +P++F  H+ ++R  + RA+M+ETGE IDW   E+LAF +L+ EG+ +RLSGQDVERG
Sbjct:  620 VPKDFHVHRTIQRFLDNRAKMMETGEGIDWATAESLAFGSLVAEGSPIRLSGQDVERG 677
[Close]
BLAST of GR915685 vs. SwissProt
Match: ODO1_BRUME (2-oxoglutarate dehydrogenase E1 component OS=Brucella melitensis GN=sucA PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.381e-12
Identity = 31/58 (53.45%), Postives = 44/58 (75.86%), Query Frame = 2 
Query:   32 IPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +P++F  H+ ++R  + RA+M+ETGE IDW   E+LAF +L VE + +RLSGQDVERG
Sbjct:  624 VPKDFHVHRTIQRFLDNRAKMMETGEGIDWATAESLAFGSLAVEAHPIRLSGQDVERG 681
[Close]
BLAST of GR915685 vs. TrEMBL
Match: Q9FLH2_ARATH (2-oxoglutarate dehydrogenase, E1 component OS=Arabidopsis thaliana GN=At5g65750 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.416e-23
Identity = 51/60 (85.00%), Postives = 55/60 (91.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            ST PENF PH+ VKR+YEQRAQMIE+GE IDWG GEALAFATL+VEGNHVRLSGQDVERG
Sbjct:  610 STFPENFKPHRGVKRVYEQRAQMIESGEGIDWGLGEALAFATLVVEGNHVRLSGQDVERG 669
[Close]
BLAST of GR915685 vs. TrEMBL
Match: Q0WLT5_ARATH (2-oxoglutarate dehydrogenase, E1 component (Fragment) OS=Arabidopsis thaliana GN=At5g65750 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.416e-23
Identity = 51/60 (85.00%), Postives = 55/60 (91.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            ST PENF PH+ VKR+YEQRAQMIE+GE IDWG GEALAFATL+VEGNHVRLSGQDVERG
Sbjct:  258 STFPENFKPHRGVKRVYEQRAQMIESGEGIDWGLGEALAFATLVVEGNHVRLSGQDVERG 317
[Close]
BLAST of GR915685 vs. TrEMBL
Match: O49541_ARATH (Oxoglutarate dehydrogenase - like protein OS=Arabidopsis thaliana GN=F6H11.130 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.416e-23
Identity = 51/60 (85.00%), Postives = 55/60 (91.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            ST PENF PH+ VKR+YEQRAQMIE+GE IDWG GEALAFATL+VEGNHVRLSGQDVERG
Sbjct:  610 STFPENFKPHRGVKRVYEQRAQMIESGEGIDWGLGEALAFATLVVEGNHVRLSGQDVERG 669
[Close]
BLAST of GR915685 vs. TrEMBL
Match: D7MUL8_ARALY (E1 subunit of 2-oxoglutarate dehydrogenase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_496813 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.416e-23
Identity = 51/60 (85.00%), Postives = 55/60 (91.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            ST PENF PH+ VKR+YEQRAQMIE+GE IDWG GEALAFATL+VEGNHVRLSGQDVERG
Sbjct:  610 STFPENFKPHRGVKRVYEQRAQMIESGEGIDWGLGEALAFATLVVEGNHVRLSGQDVERG 669
[Close]
BLAST of GR915685 vs. TrEMBL
Match: B9HM58_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_820318 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.688e-22
Identity = 49/60 (81.67%), Postives = 56/60 (93.33%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +T+P+NF PH+AVK++Y+QRAQMIETGE IDW  GEALAFATLLVEGNHVRLSGQDVERG
Sbjct:  607 TTLPDNFKPHRAVKKVYDQRAQMIETGEGIDWAVGEALAFATLLVEGNHVRLSGQDVERG 666
[Close]
BLAST of GR915685 vs. TrEMBL
Match: B9SR46_RICCO (2-oxoglutarate dehydrogenase, putative OS=Ricinus communis GN=RCOM_0465620 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.511e-22
Identity = 50/60 (83.33%), Postives = 55/60 (91.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +TIP+NF PH+AVK++YEQRAQMIETGE IDW   EALAFATLLVEGNHVRLSGQDVERG
Sbjct:  607 TTIPDNFKPHRAVKKVYEQRAQMIETGEGIDWAVAEALAFATLLVEGNHVRLSGQDVERG 666
[Close]
BLAST of GR915685 vs. TrEMBL
Match: B9HTM3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_724772 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.511e-22
Identity = 50/60 (83.33%), Postives = 54/60 (90.00%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            +T PENF PH+AVK++YEQR QMIETGE IDW  GEALAFATLLVEGNHVRLSGQDVERG
Sbjct:  607 TTFPENFKPHRAVKKVYEQRLQMIETGEGIDWAVGEALAFATLLVEGNHVRLSGQDVERG 666
[Close]
BLAST of GR915685 vs. TrEMBL
Match: Q9ZRQ2_ARATH (2-oxoglutarate dehydrogenase, E1 subunit OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.742e-21
Identity = 51/62 (82.26%), Postives = 55/62 (88.71%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRI--YEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            ST PENF PH+ VKR+  YEQRAQMIE+GE IDWG GEALAFATL+VEGNHVRLSGQDVERG
Sbjct:  610 STFPENFKPHRGVKRVRVYEQRAQMIESGEGIDWGLGEALAFATLVVEGNHVRLSGQDVERG 671
[Close]
BLAST of GR915685 vs. TrEMBL
Match: Q9M2T8_ARATH (2-oxoglutarate dehydrogenase, E1 subunit-like protein OS=Arabidopsis thaliana GN=T22E16.70 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.275e-21
Identity = 47/60 (78.33%), Postives = 55/60 (91.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            S++PENF PH+AVK++YEQRAQMIE+GE +DW   EALAFATL+VEGNHVRLSGQDVERG
Sbjct:  606 SSLPENFKPHRAVKKVYEQRAQMIESGEGVDWALAEALAFATLVVEGNHVRLSGQDVERG 665
[Close]
BLAST of GR915685 vs. TrEMBL
Match: Q84VW8_ARATH (2-oxoglutarate dehydrogenase, E1 subunit-like protein OS=Arabidopsis thaliana GN=At3g55410 PE=2 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.275e-21
Identity = 47/60 (78.33%), Postives = 55/60 (91.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            S++PENF PH+AVK++YEQRAQMIE+GE +DW   EALAFATL+VEGNHVRLSGQDVERG
Sbjct:  606 SSLPENFKPHRAVKKVYEQRAQMIESGEGVDWALAEALAFATLVVEGNHVRLSGQDVERG 665
[Close]
BLAST of GR915685 vs. TAIR peptide
Match: AT5G65750.1 (| Symbols: | 2-oxoglutarate dehydrogenase, E1 component | chr5:26304212-26307947 FORWARD LENGTH=1025)

HSP 1 Score: 110.538 bits (275), Expect = 2.157e-25
Identity = 51/60 (85.00%), Postives = 55/60 (91.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            ST PENF PH+ VKR+YEQRAQMIE+GE IDWG GEALAFATL+VEGNHVRLSGQDVERG
Sbjct:  610 STFPENFKPHRGVKRVYEQRAQMIESGEGIDWGLGEALAFATLVVEGNHVRLSGQDVERG 669
[Close]
BLAST of GR915685 vs. TAIR peptide
Match: AT3G55410.1 (| Symbols: | 2-oxoglutarate dehydrogenase, E1 component | chr3:20541897-20545728 FORWARD LENGTH=1017)

HSP 1 Score: 105.145 bits (261), Expect = 9.062e-24
Identity = 47/60 (78.33%), Postives = 55/60 (91.67%), Query Frame = 2 
Query:   26 STIPENFTPHKAVKRIYEQRAQMIETGEDIDWGFGEALAFATLLVEGNHVRLSGQDVERG 205
            S++PENF PH+AVK++YEQRAQMIE+GE +DW   EALAFATL+VEGNHVRLSGQDVERG
Sbjct:  606 SSLPENFKPHRAVKKVYEQRAQMIESGEGVDWALAEALAFATLVVEGNHVRLSGQDVERG 665
The following BLAST results are available for this feature:
BLAST of GR915685 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
ODO1_YEAST3.286e-1460.002-oxoglutarate dehydrogenase, mitochondrial OS=Sac... [more]
ODO1_BRUSU2.782e-1355.172-oxoglutarate dehydrogenase E1 component OS=Bruce... [more]
ODO1_BRUSI2.782e-1355.172-oxoglutarate dehydrogenase E1 component OS=Bruce... [more]
ODO1_BRUO22.782e-1355.172-oxoglutarate dehydrogenase E1 component OS=Bruce... [more]
ODO1_BRUC22.782e-1355.172-oxoglutarate dehydrogenase E1 component OS=Bruce... [more]
ODO1_BRUAB2.782e-1355.172-oxoglutarate dehydrogenase E1 component OS=Bruce... [more]
ODO1_BRUA22.782e-1355.172-oxoglutarate dehydrogenase E1 component OS=Bruce... [more]
ODO1_BRUA12.782e-1355.172-oxoglutarate dehydrogenase E1 component OS=Bruce... [more]
ODO1_OCHA44.745e-1353.452-oxoglutarate dehydrogenase E1 component OS=Ochro... [more]
ODO1_BRUME1.381e-1253.452-oxoglutarate dehydrogenase E1 component OS=Bruce... [more]
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BLAST of GR915685 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9FLH2_ARATH5.416e-2385.002-oxoglutarate dehydrogenase, E1 component OS=Arab... [more]
Q0WLT5_ARATH5.416e-2385.002-oxoglutarate dehydrogenase, E1 component (Fragme... [more]
O49541_ARATH5.416e-2385.00Oxoglutarate dehydrogenase - like protein OS=Arabi... [more]
D7MUL8_ARALY5.416e-2385.00E1 subunit of 2-oxoglutarate dehydrogenase OS=Arab... [more]
B9HM58_POPTR2.688e-2281.67Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9SR46_RICCO3.511e-2283.332-oxoglutarate dehydrogenase, putative OS=Ricinus ... [more]
B9HTM3_POPTR3.511e-2283.33Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q9ZRQ2_ARATH1.742e-2182.262-oxoglutarate dehydrogenase, E1 subunit OS=Arabid... [more]
Q9M2T8_ARATH2.275e-2178.332-oxoglutarate dehydrogenase, E1 subunit-like prot... [more]
Q84VW8_ARATH2.275e-2178.332-oxoglutarate dehydrogenase, E1 subunit-like prot... [more]
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BLAST of GR915685 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G65750.12.157e-2585.00| Symbols: | 2-oxoglutarate dehydrogenase, E1 com... [more]
AT3G55410.19.062e-2478.33| Symbols: | 2-oxoglutarate dehydrogenase, E1 com... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005475Transketolase-like, pyrimidine-binding domainPFAMPF02779Transket_pyrcoord: 37..68
score: 4.7
IPR0116032-oxoglutarate dehydrogenase, E1 componentPANTHERPTHR231522-OXOGLUTARATE DEHYDROGENASEcoord: 9..68
score: 1.9
NoneNo IPR availableSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 19..68
score: 1.6

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GR915685 ID=GR915685; Name=GR915685; organism=Cicer arietinum; type=EST; length=205bp
GATTTCGAGCGGCCGCCCGGGCAGGTCAACTATCCCTGAAAATTTCACAC
CTCATAAAGCAGTGAAGAGGATTTATGAACAGCGCGCACAAATGATTGAA
ACTGGGGAAGATATTGACTGGGGATTTGGAGAGGCACTTGCTTTTGCTAC
GTTGCTTGTTGAAGGTAACCATGTTCGATTGAGTGGTCAGGATGTTGAAA
GAGGA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004591oxoglutarate dehydrogenase (succinyl-transferring) activity
GO:0030976thiamine pyrophosphate binding
Vocabulary: Biological Process
TermDefinition
GO:0006096glycolytic process
Vocabulary: INTERPRO
TermDefinition
IPR005475Transketolase-like_Pyr-bd
IPR0116032oxoglutarate_DH_E1