HO062781

EST Overview
NameHO062781
Unique NameHO062781
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length204
Libraries
Library NameType
LIBEST_026409 Chickpea drought stressed cDNA SSH library AB2cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of HO062781 vs. SwissProt
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.342e-7
Identity = 30/56 (53.57%), Postives = 38/56 (67.86%), Query Frame = -3 
Query:   38 VQTWMSAALTDEETCTDGFQDV-PECPMKSDVYNRVTRVKKFTSNALALVNSYANK 202
            V+TW+SAALTDE TC DGF     +  +K  V  +V  V + TSNALALVN++A K
Sbjct:  135 VETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNFAAK 190
[Close]
BLAST of HO062781 vs. TrEMBL
Match: A5BNB2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026597 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.297e-16
Identity = 42/55 (76.36%), Postives = 48/55 (87.27%), Query Frame = -3 
Query:   38 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVNSYANK 202
            VQTWMSAALT+EETCTDGF+DVP+  +KS+V +R   VKKFTSNALALVNSY NK
Sbjct:  154 VQTWMSAALTNEETCTDGFEDVPDGALKSEVCDRAANVKKFTSNALALVNSYVNK 208
[Close]
BLAST of HO062781 vs. TrEMBL
Match: B9SP61_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_0628770 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.212e-16
Identity = 42/58 (72.41%), Postives = 48/58 (82.76%), Query Frame = -3 
Query:   29 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVNSYANKGMS 202
            VQTWMSAALTDE+TCTDGF+DV + PMKS+V  R    KKF SNALALVN+YA KGM+
Sbjct:  151 VQTWMSAALTDEDTCTDGFEDVADGPMKSEVCQRAADAKKFVSNALALVNNYAAKGMA 208
[Close]
BLAST of HO062781 vs. TrEMBL
Match: A5BDK7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_031278 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 4.927e-16
Identity = 41/55 (74.55%), Postives = 48/55 (87.27%), Query Frame = -3 
Query:   38 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVNSYANK 202
            VQTWMSAALT+E+TCTDGF+D P+  +KS+V +RV  VKKFTSNALALVNSY NK
Sbjct:  145 VQTWMSAALTNEDTCTDGFEDAPDGAVKSEVCDRVENVKKFTSNALALVNSYVNK 199
[Close]
BLAST of HO062781 vs. TrEMBL
Match: B9HW39_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1090648 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.585e-14
Identity = 40/56 (71.43%), Postives = 46/56 (82.14%), Query Frame = -3 
Query:   35 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVNSYANKG 202
            VQTWMSAALTDE+TCTDGF+DV +  +K++V NR   VKK TSNALALVNSYA  G
Sbjct:  155 VQTWMSAALTDEDTCTDGFEDVGDGEVKTEVCNRAADVKKLTSNALALVNSYAATG 210
[Close]
BLAST of HO062781 vs. TrEMBL
Match: B9HJE1_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_419607 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.342e-13
Identity = 38/50 (76.00%), Postives = 43/50 (86.00%), Query Frame = -3 
Query:   53 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVN 202
            VQTWMSAALTDEETCTDGF+DV +  +K++V NRV   KKFTSNALALVN
Sbjct:  119 VQTWMSAALTDEETCTDGFEDVADGAVKTEVCNRVADAKKFTSNALALVN 168
[Close]
BLAST of HO062781 vs. TrEMBL
Match: Q9ZNU5_ARATH (At2g01610 OS=Arabidopsis thaliana GN=At2g01610 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.660e-13
Identity = 38/57 (66.67%), Postives = 47/57 (82.46%), Query Frame = -3 
Query:   35 VQTWMSAALTDEETCTDGFQDVPECPM-KSDVYNRVTRVKKFTSNALALVNSYANKG 202
            VQTWMSAALTDE+TCTDGF+D+ E  + K+ V +R+  VK+ TSNALALVN+YAN G
Sbjct:  164 VQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRLEEVKRLTSNALALVNTYANNG 220
[Close]
BLAST of HO062781 vs. TrEMBL
Match: Q8LC39_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.660e-13
Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = -3 
Query:   32 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVNSYANKGM 202
            VQTW+SAALTDEETCTDGF+D+ + P K D+  RV  VKK TSNALALVN   +K +
Sbjct:  148 VQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDKAI 204
[Close]
BLAST of HO062781 vs. TrEMBL
Match: O49297_ARATH (At1g23205 OS=Arabidopsis thaliana GN=At1g23205 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.660e-13
Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = -3 
Query:   32 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVNSYANKGM 202
            VQTW+SAALTDEETCTDGF+D+ + P K D+  RV  VKK TSNALALVN   +K +
Sbjct:  148 VQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDKAI 204
[Close]
BLAST of HO062781 vs. TrEMBL
Match: D7LLT9_ARALY (Invertase/pectin methylesterase inhibitor family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_904368 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.660e-13
Identity = 38/57 (66.67%), Postives = 47/57 (82.46%), Query Frame = -3 
Query:   35 VQTWMSAALTDEETCTDGFQDVPECPM-KSDVYNRVTRVKKFTSNALALVNSYANKG 202
            VQTWMSAALTDE+TCTDGF+D+ E  + K+ V +R+  VK+ TSNALALVN+YAN G
Sbjct:  167 VQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRLEEVKRLTSNALALVNTYANNG 223
[Close]
BLAST of HO062781 vs. TrEMBL
Match: D7KMQ7_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472578 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.660e-13
Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = -3 
Query:   32 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVNSYANKGM 202
            VQTW+SAALTDEETCTDGF+D+ + P K D+  RV  VKK TSNALALVN   +K +
Sbjct:  148 VQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDKAI 204
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT2G01610.1 (| Symbols: | Plant invertase/pectin methylesterase inhibitor superfamily protein | chr2:274124-274792 REVERSE LENGTH=222)

HSP 1 Score: 77.0258 bits (188), Expect = 2.650e-15
Identity = 38/57 (66.67%), Postives = 47/57 (82.46%), Query Frame = -3 
Query:   35 VQTWMSAALTDEETCTDGFQDVPECPM-KSDVYNRVTRVKKFTSNALALVNSYANKG 202
            VQTWMSAALTDE+TCTDGF+D+ E  + K+ V +R+  VK+ TSNALALVN+YAN G
Sbjct:  164 VQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRLEEVKRLTSNALALVNTYANNG 220
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT1G23205.1 (| Symbols: | Plant invertase/pectin methylesterase inhibitor superfamily protein | chr1:8234234-8234851 REVERSE LENGTH=205)

HSP 1 Score: 77.0258 bits (188), Expect = 2.650e-15
Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = -3 
Query:   32 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVNSYANKGM 202
            VQTW+SAALTDEETCTDGF+D+ + P K D+  RV  VKK TSNALALVN   +K +
Sbjct:  148 VQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDKAI 204
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT1G14890.1 (| Symbols: | Plant invertase/pectin methylesterase inhibitor superfamily protein | chr1:5137045-5137704 FORWARD LENGTH=219)

HSP 1 Score: 74.3294 bits (181), Expect = 1.718e-14
Identity = 37/57 (64.91%), Postives = 46/57 (80.70%), Query Frame = -3 
Query:   35 VQTWMSAALTDEETCTDGF-QDVPECPMKSDVYNRVTRVKKFTSNALALVNSYANKG 202
            VQTWMSAALTDEETCTDG  +++ +   K+ + +RV  VK+FTSNALALVN+YAN G
Sbjct:  162 VQTWMSAALTDEETCTDGVTEEMEDGETKTAICDRVADVKRFTSNALALVNTYANNG 218
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT1G70720.1 (| Symbols: | Plant invertase/pectin methylesterase inhibitor superfamily protein | chr1:26666427-26667029 FORWARD LENGTH=200)

HSP 1 Score: 72.7886 bits (177), Expect = 4.997e-14
Identity = 38/58 (65.52%), Postives = 46/58 (79.31%), Query Frame = -3 
Query:   32 VQTWMSAALTDEETCTDGFQDVPE-CPMKSDVYNRVTRVKKFTSNALALVNSYANKGM 202
            V+TW+SAALTDE TCTDGF+DV E   +K DV +RV  VKK TSNALALVN YA++ +
Sbjct:  141 VKTWLSAALTDEYTCTDGFKDVHEDDSIKDDVCSRVDDVKKLTSNALALVNRYADESI 198
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT4G12390.1 (| Symbols: PME1 | pectin methylesterase inhibitor 1 | chr4:7336553-7337173 FORWARD LENGTH=206)

HSP 1 Score: 58.9214 bits (141), Expect = 7.468e-10
Identity = 32/59 (54.24%), Postives = 40/59 (67.80%), Query Frame = -3 
Query:   29 VQTWMSAALTDEETCTDGFQ-DVPECPMKSDVYNRVTRVKKFTSNALALVNSYANKGMS 202
            V+TW+SAALTDE TC DGF   V +  +KS +  RV  V + TSNALALVN +A +  S
Sbjct:  148 VETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALALVNRFAARHKS 206
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT5G20740.1 (| Symbols: | Plant invertase/pectin methylesterase inhibitor superfamily protein | chr5:7025867-7026484 REVERSE LENGTH=205)

HSP 1 Score: 57.3806 bits (137), Expect = 2.173e-9
Identity = 26/49 (53.06%), Postives = 37/49 (75.51%), Query Frame = -3 
Query:   53 QTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVN 199
            QTW SAALTD++TC DGFQ + +  +K++V   +T+V + TSNAL +VN
Sbjct:  143 QTWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVN 191
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT1G62770.1 (| Symbols: | Plant invertase/pectin methylesterase inhibitor superfamily protein | chr1:23246085-23246836 REVERSE LENGTH=204)

HSP 1 Score: 55.4546 bits (132), Expect = 8.256e-9
Identity = 30/56 (53.57%), Postives = 37/56 (66.07%), Query Frame = -3 
Query:   38 VQTWMSAALTDEETCTDGFQD-VPECPMKSDVYNRVTRVKKFTSNALALVNSYANK 202
            VQTW+SAALTDE TC DGF +      +K  +  +V  V + TSNALALVN +A K
Sbjct:  143 VQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALALVNQFAEK 198
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT1G62760.1 (| Symbols: | Plant invertase/pectin methylesterase inhibitor superfamily protein | chr1:23237574-23238512 REVERSE LENGTH=312)

HSP 1 Score: 55.0694 bits (131), Expect = 1.078e-8
Identity = 28/54 (51.85%), Postives = 38/54 (70.37%), Query Frame = -3 
Query:   41 VQTWMSAALTDEETCTDGFQDVPECPMKSDVYNRVTRVKKFTSNALALVNSYAN 202
            V+TW+SAALT+++TC DGF  V +  +K  V   V  V + TSNALAL+N YA+
Sbjct:  254 VETWVSAALTNDDTCMDGFSLV-KTAVKDLVRRHVVEVARLTSNALALINMYAS 306
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT5G62350.1 (| Symbols: | Plant invertase/pectin methylesterase inhibitor superfamily protein | chr5:25037504-25038112 FORWARD LENGTH=202)

HSP 1 Score: 53.9138 bits (128), Expect = 2.402e-8
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = -3 
Query:   44 QTWMSAALTDEETCTDGFQD-VPECPMKSDVYNRVTRVKKFTSNALALVNSYA 199
            QTW SAALTDE TC+DGF   V +  +K+ V  R+  V   TSNAL+L+N++A
Sbjct:  147 QTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFA 199
[Close]
BLAST of HO062781 vs. TAIR peptide
Match: AT5G62360.1 (| Symbols: | Plant invertase/pectin methylesterase inhibitor superfamily protein | chr5:25040699-25041310 FORWARD LENGTH=203)

HSP 1 Score: 53.1434 bits (126), Expect = 4.098e-8
Identity = 27/55 (49.09%), Postives = 38/55 (69.09%), Query Frame = -3 
Query:   41 VQTWMSAALTDEETCTDGFQ-DVPECPMKSDVYNRVTRVKKFTSNALALVNSYAN 202
            +QTW+SAALTD  TCTDGF+ +  +  +K  V  R+  +   TSNALAL+N +A+
Sbjct:  146 IQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINHFAS 200
The following BLAST results are available for this feature:
BLAST of HO062781 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
21KD_DAUCA1.342e-753.5721 kDa protein OS=Daucus carota PE=2 SV=1[more]
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BLAST of HO062781 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
A5BNB2_VITVI1.297e-1676.36Putative uncharacterized protein OS=Vitis vinifera... [more]
B9SP61_RICCO2.212e-1672.4121 kDa protein, putative OS=Ricinus communis GN=RC... [more]
A5BDK7_VITVI4.927e-1674.55Putative uncharacterized protein OS=Vitis vinifera... [more]
B9HW39_POPTR1.585e-1471.43Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9HJE1_POPTR1.342e-1376.00Predicted protein (Fragment) OS=Populus trichocarp... [more]
Q9ZNU5_ARATH6.660e-1366.67At2g01610 OS=Arabidopsis thaliana GN=At2g01610 PE=... [more]
Q8LC39_ARATH6.660e-1364.91Putative uncharacterized protein OS=Arabidopsis th... [more]
O49297_ARATH6.660e-1364.91At1g23205 OS=Arabidopsis thaliana GN=At1g23205 PE=... [more]
D7LLT9_ARALY6.660e-1366.67Invertase/pectin methylesterase inhibitor family p... [more]
D7KMQ7_ARALY6.660e-1364.91Putative uncharacterized protein OS=Arabidopsis ly... [more]
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BLAST of HO062781 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT2G01610.12.650e-1566.67| Symbols: | Plant invertase/pectin methylesteras... [more]
AT1G23205.12.650e-1564.91| Symbols: | Plant invertase/pectin methylesteras... [more]
AT1G14890.11.718e-1464.91| Symbols: | Plant invertase/pectin methylesteras... [more]
AT1G70720.14.997e-1465.52| Symbols: | Plant invertase/pectin methylesteras... [more]
AT4G12390.17.468e-1054.24| Symbols: PME1 | pectin methylesterase inhibitor ... [more]
AT5G20740.12.173e-953.06| Symbols: | Plant invertase/pectin methylesteras... [more]
AT1G62770.18.256e-953.57| Symbols: | Plant invertase/pectin methylesteras... [more]
AT1G62760.11.078e-851.85| Symbols: | Plant invertase/pectin methylesteras... [more]
AT5G62350.12.402e-852.83| Symbols: | Plant invertase/pectin methylesteras... [more]
AT5G62360.14.098e-849.09| Symbols: | Plant invertase/pectin methylesteras... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitorGENE3D1.20.140.40no descriptioncoord: 1..52
score: 4.5
IPR006501Pectinesterase inhibitorPFAMPF04043PMEIcoord: 1..48
score: 9.4
IPR006501Pectinesterase inhibitorSUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 1..55
score: 3.8

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO062781 ID=HO062781; Name=HO062781; organism=Cicer arietinum; type=EST; length=204bp
GTAATCAAATCCAACGGTCATAAATTCACGACATTCCTTTGTTAGCGTAA
CTATTAACGAGTGCCAAAGCATTACTCGTGAACTTCTTCACTCTGGTGAC
ACGATTGTATACATCCGATTTCATCGGACATTCCGGTACATCCTGAAATC
CGTCCGTACACGTTTCTTCATCAGTAAGCGCCGCGCTCATCCACGTCTGT
ACCT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO:0030599pectinesterase activity
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom