GR396094

EST Overview
NameGR396094
Unique NameGR396094
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length485
Libraries
Library NameType
LIBEST_024781 ICC4958 dehydration stressed root cDNA librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of GR396094 vs. TrEMBL
Match: Q5BLY1_ACTCH (Plastid alpha-amylase OS=Actinidia chinensis GN=AMY3 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.429e-15
Identity = 35/51 (68.63%), Postives = 46/51 (90.20%), Query Frame = -3 
Query:  271 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWEA 423
            ++I+KAERDVYAAIID+K+AMKIGPG +EP+S   +WSLA++G DYK+WEA
Sbjct:  844 IQITKAERDVYAAIIDKKVAMKIGPGFYEPASGPQRWSLAVEGNDYKVWEA 894
[Close]
BLAST of GR396094 vs. TrEMBL
Match: B9S2B5_RICCO (Alpha-amylase, putative OS=Ricinus communis GN=RCOM_0697970 PE=4 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.429e-15
Identity = 38/51 (74.51%), Postives = 46/51 (90.20%), Query Frame = -3 
Query:  271 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWEA 423
            V+I+KAERDVYAAII+EK+AMKIGPGH+EP S    WS+AI+GKDYK+WEA
Sbjct:  850 VKITKAERDVYAAIIEEKVAMKIGPGHYEPPSG-KNWSMAIEGKDYKVWEA 899
[Close]
BLAST of GR396094 vs. TrEMBL
Match: Q9FUA8_ARATH (PRLI-interacting factor E (Fragment) OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.608e-14
Identity = 34/50 (68.00%), Postives = 43/50 (86.00%), Query Frame = -3 
Query:  274 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWE 423
            V I K+ERDVYAAIIDEK+AMKIGPGH+EP +    WS+A++G+DYK+WE
Sbjct:   69 VNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 118
[Close]
BLAST of GR396094 vs. TrEMBL
Match: Q9CAR6_ARATH (Putative alpha-amylase; 60344-64829 OS=Arabidopsis thaliana GN=T17F3.14 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.608e-14
Identity = 34/50 (68.00%), Postives = 43/50 (86.00%), Query Frame = -3 
Query:  274 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWE 423
            V I K+ERDVYAAIIDEK+AMKIGPGH+EP +    WS+A++G+DYK+WE
Sbjct:  775 VNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 824
[Close]
BLAST of GR396094 vs. TrEMBL
Match: Q94A41_ARATH (At1g69830/T17F3_14 OS=Arabidopsis thaliana GN=At1g69830 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.608e-14
Identity = 34/50 (68.00%), Postives = 43/50 (86.00%), Query Frame = -3 
Query:  274 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWE 423
            V I K+ERDVYAAIIDEK+AMKIGPGH+EP +    WS+A++G+DYK+WE
Sbjct:  836 VNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885
[Close]
BLAST of GR396094 vs. TrEMBL
Match: Q5BLY3_MALDO (Plastid alpha-amylase OS=Malus domestica GN=AMY10 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.743e-14
Identity = 34/51 (66.67%), Postives = 46/51 (90.20%), Query Frame = -3 
Query:  271 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWEA 423
            V+I+KAERDVYAAIIDEK+A+KIGPGH+EP+S    W+ +++G+DYK+WEA
Sbjct:  850 VKITKAERDVYAAIIDEKVAIKIGPGHYEPASGPQNWNKSLEGRDYKVWEA 900
[Close]
BLAST of GR396094 vs. TrEMBL
Match: D7TNE3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_26.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00020069001 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.679e-14
Identity = 34/50 (68.00%), Postives = 44/50 (88.00%), Query Frame = -3 
Query:  274 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWE 423
            ++I+ AERDVYAAIIDEK+AMKIGPG++EP     +W+LA++GKDYKIWE
Sbjct:  834 IQITMAERDVYAAIIDEKVAMKIGPGYYEPPKGQQRWTLALEGKDYKIWE 883
[Close]
BLAST of GR396094 vs. TrEMBL
Match: D7KXK5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_339218 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.679e-14
Identity = 33/50 (66.00%), Postives = 43/50 (86.00%), Query Frame = -3 
Query:  274 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWE 423
            V I K+ERDVYAAIID+K+AMKIGPGH+EP +    WS+A++G+DYK+WE
Sbjct:  831 VNIDKSERDVYAAIIDDKVAMKIGPGHYEPPNGSKNWSVAVEGRDYKVWE 880
[Close]
BLAST of GR396094 vs. TrEMBL
Match: A5BAB3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_001008 PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.679e-14
Identity = 34/50 (68.00%), Postives = 44/50 (88.00%), Query Frame = -3 
Query:  274 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWE 423
            ++I+ AERDVYAAIIDEK+AMKIGPG++EP     +W+LA++GKDYKIWE
Sbjct:  836 IQITMAERDVYAAIIDEKVAMKIGPGYYEPPKGQQRWTLALEGKDYKIWE 885
[Close]
BLAST of GR396094 vs. TrEMBL
Match: Q0JJV2_ORYSJ (Os01g0715400 protein OS=Oryza sativa subsp. japonica GN=Os01g0715400 PE=2 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 1.010e-8
Identity = 28/50 (56.00%), Postives = 39/50 (78.00%), Query Frame = -3 
Query:  274 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWE 423
            ++I KAE ++YAA IDE++ MKIG GHFEPS   + W +A +G+DYK+WE
Sbjct:  510 IKILKAEGNLYAAEIDERVTMKIGAGHFEPSGPTN-WVVAAEGQDYKVWE 558
[Close]
BLAST of GR396094 vs. TAIR peptide
Match: AT1G69830.1 (| Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 | chr1:26288518-26293003 REVERSE LENGTH=887)

HSP 1 Score: 82.4185 bits (202), Expect = 1.332e-16
Identity = 34/50 (68.00%), Postives = 43/50 (86.00%), Query Frame = -3 
Query:  274 VEISKAERDVYAAIIDEKIAMKIGPGHFEPSSTFHKWSLAIDGKDYKIWE 423
            V I K+ERDVYAAIIDEK+AMKIGPGH+EP +    WS+A++G+DYK+WE
Sbjct:  836 VNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885
The following BLAST results are available for this feature:
BLAST of GR396094 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q5BLY1_ACTCH9.429e-1568.63Plastid alpha-amylase OS=Actinidia chinensis GN=AM... [more]
B9S2B5_RICCO9.429e-1574.51Alpha-amylase, putative OS=Ricinus communis GN=RCO... [more]
Q9FUA8_ARATH1.608e-1468.00PRLI-interacting factor E (Fragment) OS=Arabidopsi... [more]
Q9CAR6_ARATH1.608e-1468.00Putative alpha-amylase; 60344-64829 OS=Arabidopsis... [more]
Q94A41_ARATH1.608e-1468.00At1g69830/T17F3_14 OS=Arabidopsis thaliana GN=At1g... [more]
Q5BLY3_MALDO2.743e-1466.67Plastid alpha-amylase OS=Malus domestica GN=AMY10 ... [more]
D7TNE3_VITVI4.679e-1468.00Whole genome shotgun sequence of line PN40024, sca... [more]
D7KXK5_ARALY4.679e-1466.00Putative uncharacterized protein OS=Arabidopsis ly... [more]
A5BAB3_VITVI4.679e-1468.00Putative uncharacterized protein OS=Vitis vinifera... [more]
Q0JJV2_ORYSJ1.010e-856.00Os01g0715400 protein OS=Oryza sativa subsp. japoni... [more]
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BLAST of GR396094 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT1G69830.11.332e-1668.00| Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 | c... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 1..50
score: 1.7
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810no descriptioncoord: 1..51
score: 6.5
IPR013780Glycosyl hydrolase, family 13, all-betaGENE3D2.60.40.1180no descriptioncoord: 1..50
score: 5.7
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 1..51
score: 1.3

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GR396094 ID=GR396094; Name=GR396094; organism=Cicer arietinum; type=EST; length=485bp
ATTTTTGTTATTGAAATATTTTAAAAACTAAATCAGTTTTATTTATATTT
ATTCGAACCTTATATTTCAAAATTTTGAAAATTCTCGTTTTATAAATCCA
TGTACATGGCTATATACAATAAGCAACCTTGTGGCAACACTCACTTAAAT
TGGAGCTAGCCTGCCATACACCATCAGAAGAAATCACATCCTATTATTGA
CTTATAAGAACTACAATGCAGTGGATTTACATGGAATGATGGAACTATAT
AGTATATAACATTATGATGATGCTTCCCAAATCTTATAGTCTTTTCCATC
AATAGCAAGGGACCATTTGTGGAAGGTACTAGAGGGTTCAAAATGTCCAG
GTCCAATCTTCATCGCGATTTTTTCATCGATGATGGCGGCATAAACATCC
CTCTCTGCCTTACTAATTTCAACCTAAGGCATCAAACATGAAAGGTTTAG
TTAGTTATGATACAACAATATCAATGTACAGAAAA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004556alpha-amylase activity
GO:0005509calcium ion binding
GO:0003824catalytic activity
GO:0043169cation binding
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR012850A-amylase_bs_C
IPR013780Glyco_hydro_b