GR407048

EST Overview


Name	GR407048
Unique Name	GR407048
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	289

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR407048 vs. SwissProt
Match: XTH29_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis thaliana GN=XTH29 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.745e-15
Identity = 35/51 (68.63%), Postives = 45/51 (88.24%), Query Frame = 1

Query:  136 FNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            F++G SHLFG+GN++RS D   V+LLLD+YTGSGFISS+MYQ+GFFS+ IK
Sbjct:   40 FDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGSGFISSSMYQHGFFSSLIK 90

[Close]

BLAST of GR407048 vs. SwissProt
Match: XTH30_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis thaliana GN=XTH30 PE=2 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 2.563e-14
Identity = 38/61 (62.30%), Postives = 47/61 (77.05%), Query Frame = 1

Query:  106 NAAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            +A  +L T+ F +  S LFGD N+VRS D   V+LLLDRYTGSGFISSNMYQ+GF+S+ IK
Sbjct:   22 SAFTNLNTLSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGSGFISSNMYQHGFYSSMIK 82

[Close]

BLAST of GR407048 vs. SwissProt
Match: XTH28_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis thaliana GN=XTH28 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.718e-11
Identity = 29/56 (51.79%), Postives = 42/56 (75.00%), Query Frame = 1

Query:  121 LATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            L  I F++GY+ LFGD N++   DG  V+L LD  TGSGF+S+++Y +GFFS++IK
Sbjct:   26 LPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGSGFVSNDIYLHGFFSSSIK 81

[Close]

BLAST of GR407048 vs. SwissProt
Match: XTH27_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis thaliana GN=XTH27 PE=2 SV=2)

HSP 1 Score: 61.6178 bits (148), Expect = 1.455e-9
Identity = 30/63 (47.62%), Postives = 41/63 (65.08%), Query Frame = 1

Query:  109 AAFDLATIP---FNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            + F L  +P   F + Y+ LFGD N+    DG  V+L LD  TGSGF+S++ Y +GFFSA+IK
Sbjct:   19 SGFALQNLPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGSGFVSNDYYLHGFFSASIK 81

[Close]

BLAST of GR407048 vs. TrEMBL
Match: C6THS0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 1.301e-24
Identity = 63/93 (67.74%), Postives = 66/93 (70.97%), Query Frame = 1

Query:   13 MDH-SSLRCFGTIPKTHXXXXXXXXXXXXXXXNAAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            MDH   LRCFGTIPK                 NA+FDLATIPFNDGYS LFG  NVVRS DG GV+L LDRYTGSGFISS+MYQYGFFSA IK
Sbjct:    1 MDHIHRLRCFGTIPKI--PFLLLLSLLLFSFVNASFDLATIPFNDGYSPLFGASNVVRSEDGYGVQLRLDRYTGSGFISSSMYQYGFFSARIK 91

[Close]

BLAST of GR407048 vs. TrEMBL
Match: D7TTQ4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_12.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00013055001 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.819e-18
Identity = 42/60 (70.00%), Postives = 54/60 (90.00%), Query Frame = 1

Query:  109 AAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            ++F+L+ I F+ GY+HLFG+GN+VRSSDG  V+LLLDRYTGSGFIS+N+Y +GFFSANIK
Sbjct:   25 SSFNLSVISFDQGYTHLFGEGNLVRSSDGRSVRLLLDRYTGSGFISANLYNHGFFSANIK 84

[Close]

BLAST of GR407048 vs. TrEMBL
Match: B9RZ21_RICCO (Xyloglucan endotransglucosylase/hydrolase protein 2, putative OS=Ricinus communis GN=RCOM_1316740 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.819e-18
Identity = 44/60 (73.33%), Postives = 54/60 (90.00%), Query Frame = 1

Query:  109 AAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            AAF+L+TI F++GY+ LFGDGN+VRS +G  V+LLLDR+TGSGFISS MY+YGFFSANIK
Sbjct:   32 AAFNLSTISFDEGYTPLFGDGNLVRSPNGRSVRLLLDRFTGSGFISSKMYEYGFFSANIK 91

[Close]

BLAST of GR407048 vs. TrEMBL
Match: A2TEJ0_9ROSI (Xyloglucan endotransglycosylase/hydrolase XTH-3 OS=Populus tremula x Populus tremuloides PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.819e-18
Identity = 44/60 (73.33%), Postives = 53/60 (88.33%), Query Frame = 1

Query:  109 AAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            AAF+L+TI F++GYS LFGDGN+VRS DG   +LLLDR+TG+GFISS MY+YGFFSANIK
Sbjct:   28 AAFNLSTISFDEGYSPLFGDGNLVRSPDGRSARLLLDRFTGAGFISSRMYKYGFFSANIK 87

[Close]

BLAST of GR407048 vs. TrEMBL
Match: B9MU78_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_423757 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.376e-18
Identity = 44/61 (72.13%), Postives = 54/61 (88.52%), Query Frame = 1

Query:  106 NAAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            +AAF+L+TI F++GYS LFGDGN+VRS DG   +LLLDR+TG+GFISS MY+YGFFSANIK
Sbjct:    5 SAAFNLSTISFDEGYSPLFGDGNLVRSPDGRTARLLLDRFTGAGFISSKMYKYGFFSANIK 65

[Close]

BLAST of GR407048 vs. TrEMBL
Match: C0IRH3_ACTDE (Xyloglucan endotransglucosylase/hydrolase 14 OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 3.211e-15
Identity = 40/61 (65.57%), Postives = 49/61 (80.33%), Query Frame = 1

Query:  106 NAAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            +A F++ATIPFN GY  LFGD NVVRSSD   V L+L++YTGSGF SS++Y +GFFSA IK
Sbjct:   26 HAEFNIATIPFNKGYYPLFGDNNVVRSSDDKSVNLVLNQYTGSGFKSSDLYNHGFFSAKIK 86

[Close]

BLAST of GR407048 vs. TrEMBL
Match: C0PL00_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.154e-15
Identity = 39/60 (65.00%), Postives = 50/60 (83.33%), Query Frame = 1

Query:  109 AAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            +AFD+ T+ F +G+S LFGDGN+VRSSD   V+LLLDR +GSGFISS+ Y +GFFSA+IK
Sbjct:   27 SAFDVPTVAFEEGFSPLFGDGNLVRSSDDRSVRLLLDRRSGSGFISSDYYLHGFFSASIK 86

[Close]

BLAST of GR407048 vs. TrEMBL
Match: B4FE99_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.154e-15
Identity = 39/60 (65.00%), Postives = 50/60 (83.33%), Query Frame = 1

Query:  109 AAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            +AFD+ T+ F +G+S LFGDGN+VRSSD   V+LLLDR +GSGFISS+ Y +GFFSA+IK
Sbjct:   27 SAFDVPTVAFEEGFSPLFGDGNLVRSSDDRSVRLLLDRRSGSGFISSDYYLHGFFSASIK 86

[Close]

BLAST of GR407048 vs. TrEMBL
Match: B4FAV6_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.154e-15
Identity = 39/60 (65.00%), Postives = 50/60 (83.33%), Query Frame = 1

Query:  109 AAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            +AFD+ T+ F +G+S LFGDGN+VRSSD   V+LLLDR +GSGFISS+ Y +GFFSA+IK
Sbjct:   27 SAFDVPTVAFEEGFSPLFGDGNLVRSSDDRSVRLLLDRRSGSGFISSDYYLHGFFSASIK 86

[Close]

BLAST of GR407048 vs. TrEMBL
Match: C5XTQ9_SORBI (Putative uncharacterized protein Sb04g002290 OS=Sorghum bicolor GN=Sb04g002290 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.637e-14
Identity = 38/59 (64.41%), Postives = 48/59 (81.36%), Query Frame = 1

Query:  112 AFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            A ++ T+ F DGY+ LFG GN++RS+D   V LLLDR TGSGFISS+MYQ+GFFSA+IK
Sbjct:   36 AINVTTMAFEDGYAPLFGHGNILRSADHRTVSLLLDRSTGSGFISSSMYQHGFFSASIK 94

[Close]

BLAST of GR407048 vs. TAIR peptide
Match: AT4G18990.1 (| Symbols: XTH29 | xyloglucan endotransglucosylase/hydrolase 29 | chr4:10401941-10404248 REVERSE LENGTH=357)

HSP 1 Score: 79.337 bits (194), Expect = 5.303e-16
Identity = 35/51 (68.63%), Postives = 45/51 (88.24%), Query Frame = 1

Query:  136 FNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            F++G SHLFG+GN++RS D   V+LLLD+YTGSGFISS+MYQ+GFFS+ IK
Sbjct:   40 FDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGSGFISSSMYQHGFFSSLIK 90

[Close]

BLAST of GR407048 vs. TAIR peptide
Match: AT1G32170.1 (| Symbols: XTR4, XTH30 | xyloglucan endotransglucosylase/hydrolase 30 | chr1:11575434-11577776 FORWARD LENGTH=343)

HSP 1 Score: 77.411 bits (189), Expect = 2.015e-15
Identity = 38/61 (62.30%), Postives = 47/61 (77.05%), Query Frame = 1

Query:  106 NAAFDLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            +A  +L T+ F +  S LFGD N+VRS D   V+LLLDRYTGSGFISSNMYQ+GF+S+ IK
Sbjct:   22 SAFTNLNTLSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGSGFISSNMYQHGFYSSMIK 82

[Close]

BLAST of GR407048 vs. TAIR peptide
Match: AT1G14720.1 (| Symbols: XTR2, EXGT-A2, ATXTH28, XTH28 | xyloglucan endotransglucosylase/hydrolase 28 | chr1:5066806-5068466 REVERSE LENGTH=332)

HSP 1 Score: 65.855 bits (159), Expect = 6.068e-12
Identity = 29/56 (51.79%), Postives = 42/56 (75.00%), Query Frame = 1

Query:  121 LATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            L  I F++GY+ LFGD N++   DG  V+L LD  TGSGF+S+++Y +GFFS++IK
Sbjct:   26 LPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGSGFVSNDIYLHGFFSSSIK 81

[Close]

BLAST of GR407048 vs. TAIR peptide
Match: AT2G01850.1 (| Symbols: EXGT-A3, XTH27, ATXTH27 | endoxyloglucan transferase A3 | chr2:385374-387138 FORWARD LENGTH=333)

HSP 1 Score: 61.6178 bits (148), Expect = 1.144e-10
Identity = 30/63 (47.62%), Postives = 41/63 (65.08%), Query Frame = 1

Query:  109 AAFDLATIP---FNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            + F L  +P   F + Y+ LFGD N+    DG  V+L LD  TGSGF+S++ Y +GFFSA+IK
Sbjct:   19 SGFALQNLPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGSGFVSNDYYLHGFFSASIK 81

[Close]

BLAST of GR407048 vs. TAIR peptide
Match: AT4G13080.1 (| Symbols: XTH1 | xyloglucan endotransglucosylase/hydrolase 1 | chr4:7626612-7628367 REVERSE LENGTH=292)

HSP 1 Score: 50.0618 bits (118), Expect = 3.446e-7
Identity = 24/57 (42.11%), Postives = 34/57 (59.65%), Query Frame = 1

Query:  118 DLATIPFNDGYSHLFGDGNVVRSSDGNGVKLLLDRYTGSGFISSNMYQYGFFSANIK 288
            D + + F+D Y   +G  NV++ + G  V+L LD  +GSGF S N Y+ GFF   IK
Sbjct:   30 DQSKVGFDDNYVVTWGQNNVLKLNQGKEVQLSLDHSSGSGFESKNHYESGFFQIRIK 86

The following BLAST results are available for this feature:

BLAST of GR407048 vs. SwissProt
BLAST of GR407048 vs. TrEMBL
BLAST of GR407048 vs. TAIR peptide

BLAST of GR407048 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
XTH29_ARATH	6.745e-15	68.63	Probable xyloglucan endotransglucosylase/hydrolase...	[more]
XTH30_ARATH	2.563e-14	62.30	Probable xyloglucan endotransglucosylase/hydrolase...	[more]
XTH28_ARATH	7.718e-11	51.79	Probable xyloglucan endotransglucosylase/hydrolase...	[more]
XTH27_ARATH	1.455e-9	47.62	Probable xyloglucan endotransglucosylase/hydrolase...	[more]

BLAST of GR407048 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6THS0_SOYBN	1.301e-24	67.74	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7TTQ4_VITVI	1.819e-18	70.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9RZ21_RICCO	1.819e-18	73.33	Xyloglucan endotransglucosylase/hydrolase protein ...	[more]
A2TEJ0_9ROSI	1.819e-18	73.33	Xyloglucan endotransglycosylase/hydrolase XTH-3 OS...	[more]
B9MU78_POPTR	2.376e-18	72.13	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
C0IRH3_ACTDE	3.211e-15	65.57	Xyloglucan endotransglucosylase/hydrolase 14 OS=Ac...	[more]
C0PL00_MAIZE	7.154e-15	65.00	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B4FE99_MAIZE	7.154e-15	65.00	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B4FAV6_MAIZE	7.154e-15	65.00	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C5XTQ9_SORBI	4.637e-14	64.41	Putative uncharacterized protein Sb04g002290 OS=So...	[more]

BLAST of GR407048 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G18990.1	5.303e-16	68.63	\| Symbols: XTH29 \| xyloglucan endotransglucosylase...	[more]
AT1G32170.1	2.015e-15	62.30	\| Symbols: XTR4, XTH30 \| xyloglucan endotransgluco...	[more]
AT1G14720.1	6.068e-12	51.79	\| Symbols: XTR2, EXGT-A2, ATXTH28, XTH28 \| xyloglu...	[more]
AT2G01850.1	1.144e-10	47.62	\| Symbols: EXGT-A3, XTH27, ATXTH27 \| endoxylogluca...	[more]
AT4G13080.1	3.446e-7	42.11	\| Symbols: XTH1 \| xyloglucan endotransglucosylase/...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000757	Glycoside hydrolase, family 16	PFAM	PF00722	Glyco_hydro_16	coord: 48..96 score: 1.9
IPR008985	Concanavalin A-like lectin/glucanase	SUPERFAMILY	49899	Concanavalin A-like lectins/glucanases	coord: 42..96 score: 8.4
IPR013320	Concanavalin A-like lectin/glucanase, subgroup	GENE3D	2.60.120.200	no description	coord: 36..96 score: 3.2
None	No IPR available	SEG	seg	seg	coord: 21..35 score
None	No IPR available	SEG	seg	seg	coord: 21..47 score
None	No IPR available	SEG	seg	seg	coord: 11..23 score
None	No IPR available	SEG	seg	seg	coord: 50..65 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR407048 ID=GR407048; Name=GR407048; organism=Cicer arietinum; type=EST; length=289bp
CTTAAAATTAGTATGGATCATAGTAGCTTACGTTGTTTTGGGACAATACC
CAAAACTCATTTTCTTCTATTATTCTCTTTTTCTATATTTTTATTCACTT
TCACAAACGCAGCCTTCGACCTCGCAACCATACCCTTCAACGATGGCTAT
TCTCATCTCTTCGGAGATGGCAACGTTGTTCGATCCTCCGATGGCAACGG
TGTTAAACTCCTCCTTGATCGATACACCGGTTCTGGATTCATTTCCTCCA
ATATGTACCAATATGGATTCTTCAGTGCTAATATCAAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000757	GH16
IPR008985	Concanavalin A-like lectin/glucanase
IPR013320	ConA-like_dom_sf

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