GR408409

EST Overview


Name	GR408409
Unique Name	GR408409
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	271

Libraries


Library Name	Type
LIBEST_024786 JG11 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR408409 vs. SwissProt
Match: FTSH4_ARATH (Cell division protease ftsH homolog 4, mitochondrial OS=Arabidopsis thaliana GN=FTSH4 PE=1 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 1.298e-18
Identity = 45/60 (75.00%), Postives = 53/60 (88.33%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILT 182
            AT + R MVTK+GMS EVG VAHNY D+G++MS++TRLLIE+EVK LLEKAYNNAKTILT
Sbjct:  568 ATKLARAMVTKFGMSKEVGLVAHNYDDNGKSMSTETRLLIESEVKQLLEKAYNNAKTILT 627

[Close]

BLAST of GR408409 vs. SwissProt
Match: FTSH4_ORYSJ (Cell division protease ftsH homolog 4, mitochondrial OS=Oryza sativa subsp. japonica GN=FTSH4 PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.098e-17
Identity = 43/60 (71.67%), Postives = 52/60 (86.67%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILT 182
            AT M R MVTKYGMS ++G V++NY DDG++MS++TRLLIE EVK+LLE AYNNAKTILT
Sbjct:  564 ATKMARAMVTKYGMSKQLGFVSYNYEDDGKSMSTETRLLIEQEVKSLLENAYNNAKTILT 623

[Close]

BLAST of GR408409 vs. SwissProt
Match: FTSH5_ORYSJ (Cell division protease ftsH homolog 5, mitochondrial OS=Oryza sativa subsp. japonica GN=FTSH5 PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.664e-15
Identity = 38/59 (64.41%), Postives = 49/59 (83.05%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTIL 179
            AT++ R MVTKYGMS ++G V++NY DDG++MS++TRLLIE EVK  +E AYNNAK IL
Sbjct:  571 ATAVARAMVTKYGMSKQLGFVSYNYEDDGKSMSTETRLLIEKEVKCFVENAYNNAKNIL 629

[Close]

BLAST of GR408409 vs. SwissProt
Match: YME1_SCHPO (Protein YME1 homolog OS=Schizosaccharomyces pombe GN=SPCC965.04c PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 6.679e-7
Identity = 26/59 (44.07%), Postives = 41/59 (69.49%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTIL 179
            AT + R+MVT++GMS  +GPV+     D  N+S  TR L+E+E+K+LLE +Y  + ++L
Sbjct:  612 ATQVARRMVTEFGMSDRIGPVSLEAEMD--NLSPATRALVESEIKSLLEASYERSLSLL 668

[Close]

BLAST of GR408409 vs. TrEMBL
Match: Q6KC90_PEA (FtsH-like protease OS=Pisum sativum GN=ftsh4 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 5.412e-23
Identity = 58/89 (65.17%), Postives = 65/89 (73.03%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILTTXXXXXXXXXXXXXXXXXXXGSQKIDLLA 269
            ATS+ R+MVTKYGMSTEVGPV HNYYD+GR+MSS+TRLLIE EVKNLLE+AYNNAKTILTT                   GSQ  +LLA
Sbjct:  567 ATSLAREMVTKYGMSTEVGPVTHNYYDNGRSMSSETRLLIEKEVKNLLERAYNNAKTILTTHEKELHALANALLEQETLTGSQINELLA 655

[Close]

BLAST of GR408409 vs. TrEMBL
Match: B9RRQ8_RICCO (Protein YME1, putative OS=Ricinus communis GN=RCOM_0797270 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.014e-18
Identity = 46/61 (75.41%), Postives = 52/61 (85.25%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILTT 185
            AT + R MVTKYGMS EVG V HNY D+G++MS++TRLLIE EVKN LEKAYNNAKTILTT
Sbjct:  565 ATKLARAMVTKYGMSKEVGVVTHNYDDNGKSMSTETRLLIEQEVKNFLEKAYNNAKTILTT 625

[Close]

BLAST of GR408409 vs. TrEMBL
Match: D7STQ6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_8.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00035393001 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.525e-17
Identity = 45/61 (73.77%), Postives = 53/61 (86.89%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILTT 185
            ATS+ R MVTK+GMS EVG V HNY D+G++MS++TRLLIE EVK+ LEKAYNNAKTILTT
Sbjct:  587 ATSLARAMVTKFGMSKEVGVVTHNYDDNGKSMSTETRLLIEKEVKHFLEKAYNNAKTILTT 647

[Close]

BLAST of GR408409 vs. TrEMBL
Match: D7LCQ9_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_481469 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.602e-17
Identity = 45/60 (75.00%), Postives = 53/60 (88.33%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILT 182
            AT + R MVTK+GMS EVG VAHNY D+G++MS++TRLLIE+EVK LLEKAYNNAKTILT
Sbjct:  568 ATKLARAMVTKFGMSKEVGLVAHNYDDNGKSMSTETRLLIESEVKQLLEKAYNNAKTILT 627

[Close]

BLAST of GR408409 vs. TrEMBL
Match: C5XNS5_SORBI (Putative uncharacterized protein Sb03g025820 OS=Sorghum bicolor GN=Sb03g025820 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 9.886e-17
Identity = 43/60 (71.67%), Postives = 52/60 (86.67%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILT 182
            AT+M R MVTKYGMS +VG V++NY DDG+++SS+TRL+IE EVKN LE AYNNAKTILT
Sbjct:  564 ATAMARAMVTKYGMSKQVGLVSYNYEDDGKSLSSETRLVIEQEVKNFLENAYNNAKTILT 623

[Close]

BLAST of GR408409 vs. TrEMBL
Match: Q84LQ3_SOLLC (Putative FtsH protease OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.291e-16
Identity = 43/61 (70.49%), Postives = 52/61 (85.25%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILTT 185
            AT + R MVTK+GMS EVG V HNY D+G++MS++TRLLIE EV+ LLE+AYNNAKTILTT
Sbjct:  566 ATKLARTMVTKFGMSKEVGLVTHNYDDNGKSMSTETRLLIEKEVRELLERAYNNAKTILTT 626

[Close]

BLAST of GR408409 vs. TrEMBL
Match: C0PNQ4_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.202e-16
Identity = 43/60 (71.67%), Postives = 51/60 (85.00%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILT 182
            AT+M R MVTKYGMS  VG V++NY DDG+++SS+TRL+IE EVKN LE AYNNAKTILT
Sbjct:   27 ATAMARAMVTKYGMSKHVGLVSYNYEDDGKSVSSETRLVIEQEVKNFLENAYNNAKTILT 86

[Close]

BLAST of GR408409 vs. TrEMBL
Match: A2WRN7_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_02522 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.202e-16
Identity = 43/60 (71.67%), Postives = 52/60 (86.67%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILT 182
            AT M R MVTKYGMS ++G V++NY DDG++MS++TRLLIE EVK+LLE AYNNAKTILT
Sbjct:  564 ATKMARAMVTKYGMSKQLGFVSYNYEDDGKSMSTETRLLIEQEVKSLLENAYNNAKTILT 623

[Close]

BLAST of GR408409 vs. TrEMBL
Match: C0P4W8_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 2.876e-16
Identity = 43/60 (71.67%), Postives = 51/60 (85.00%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILT 182
            AT+M R MVTKYGMS +VG V++NY DDG++MSS+TRL IE EVKN LE AY+NAKTILT
Sbjct:  564 ATAMARAMVTKYGMSKQVGLVSYNYEDDGKSMSSETRLAIEQEVKNFLENAYSNAKTILT 623

[Close]

BLAST of GR408409 vs. TrEMBL
Match: D0VEB2_TOBAC (FtsH protease (Fragment) OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.757e-16
Identity = 42/61 (68.85%), Postives = 53/61 (86.89%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILTT 185
            AT++ R MVTK+GMS EVG V HNY D+G++MS++TRLLIE EV+ LLE+AYNNAKTILT+
Sbjct:   17 ATNLARAMVTKWGMSKEVGLVTHNYDDNGKSMSTETRLLIEKEVRELLERAYNNAKTILTS 77

[Close]

BLAST of GR408409 vs. TAIR peptide
Match: AT2G26140.1 (| Symbols: ftsh4 | FTSH protease 4 | chr2:11131939-11135126 REVERSE LENGTH=717)

HSP 1 Score: 91.6633 bits (226), Expect = 1.016e-19
Identity = 45/60 (75.00%), Postives = 53/60 (88.33%), Query Frame = 3

Query:    3 ATSMGRKMVTKYGMSTEVGPVAHNYYDDGRNMSSKTRLLIENEVKNLLEKAYNNAKTILT 182
            AT + R MVTK+GMS EVG VAHNY D+G++MS++TRLLIE+EVK LLEKAYNNAKTILT
Sbjct:  568 ATKLARAMVTKFGMSKEVGLVAHNYDDNGKSMSTETRLLIESEVKQLLEKAYNNAKTILT 627

The following BLAST results are available for this feature:

BLAST of GR408409 vs. SwissProt
BLAST of GR408409 vs. TrEMBL
BLAST of GR408409 vs. TAIR peptide

BLAST of GR408409 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
FTSH4_ARATH	1.298e-18	75.00	Cell division protease ftsH homolog 4, mitochondri...	[more]
FTSH4_ORYSJ	1.098e-17	71.67	Cell division protease ftsH homolog 4, mitochondri...	[more]
FTSH5_ORYSJ	6.664e-15	64.41	Cell division protease ftsH homolog 5, mitochondri...	[more]
YME1_SCHPO	6.679e-7	44.07	Protein YME1 homolog OS=Schizosaccharomyces pombe ...	[more]

BLAST of GR408409 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q6KC90_PEA	5.412e-23	65.17	FtsH-like protease OS=Pisum sativum GN=ftsh4 PE=2 ...	[more]
B9RRQ8_RICCO	4.014e-18	75.41	Protein YME1, putative OS=Ricinus communis GN=RCOM...	[more]
D7STQ6_VITVI	1.525e-17	73.77	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7LCQ9_ARALY	2.602e-17	75.00	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C5XNS5_SORBI	9.886e-17	71.67	Putative uncharacterized protein Sb03g025820 OS=So...	[more]
Q84LQ3_SOLLC	1.291e-16	70.49	Putative FtsH protease OS=Solanum lycopersicum PE=...	[more]
C0PNQ4_MAIZE	2.202e-16	71.67	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
A2WRN7_ORYSI	2.202e-16	71.67	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C0P4W8_MAIZE	2.876e-16	71.67	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
D0VEB2_TOBAC	3.757e-16	68.85	FtsH protease (Fragment) OS=Nicotiana tabacum PE=2...	[more]

BLAST of GR408409 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G26140.1	1.016e-19	75.00	\| Symbols: ftsh4 \| FTSH protease 4 \| chr2:11131939...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000642	Peptidase M41	PFAM	PF01434	Peptidase_M41	coord: 1..83 score: 2
None	No IPR available	PANTHER	PTHR23076	METALLOPROTEASE M41 FTSH	coord: 1..78 score: 2
None	No IPR available	PANTHER	PTHR23076:SF10	CELL DIVISION PROTEIN FTSH HOMOLOG	coord: 1..78 score: 2
None	No IPR available	SEG	seg	seg	coord: 62..80 score
None	No IPR available	SUPERFAMILY	140990	FtsH protease domain-like	coord: 1..89 score: 8.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR408409 ID=GR408409; Name=GR408409; organism=Cicer arietinum; type=EST; length=271bp
AGGCAACTAGTATGGGCAGGAAAATGGTTACTAAGTATGGGATGAGTACT
GAAGTTGGACCCGTTGCTCATAATTACTATGATGATGGGAGAAATATGAG
CTCAAAAACTAGGCTGCTTATTGAGAATGAAGTGAAAAACTTGCTGGAAA
AAGCTTACAACAATGCAAAAACTATTCTAACCACCCATAAAAAGGAGCTT
CATGCCCTTGCAAATGCTCTTCTGGAGCACGAAACACTTACAGGAAGTCA
GAAAATTGATTTACTTGCAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004222	metalloendopeptidase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **INTERPRO**
Term	Definition
IPR000642	Peptidase_M41

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