GR421088

EST Overview


Name	GR421088
Unique Name	GR421088
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	549

Libraries


Library Name	Type
LIBEST_024785 ICCV2 Salinity-stressed chickpea root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR421088 vs. SwissProt
Match: RPE1_ORYSJ (Ribulose-phosphate 3-epimerase, cytoplasmic isoform OS=Oryza sativa subsp. japonica GN=Os09g0505700 PE=1 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 2.659e-9
Identity = 30/52 (57.69%), Postives = 38/52 (73.08%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQN 303
            +IEVDGGLGPSTID+AA A  + +    ++ GA EP +VIS LR SVE +QN
Sbjct:  175 DIEVDGGLGPSTIDVAASAGANCIVAGSSIFGAAEPGEVISALRKSVEGSQN 226

HSP 2 Score: 21.1718 bits (43), Expect = 2.659e-9
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMPE M+KVR
Sbjct:  156 FMPEMMEKVR 165

[Close]

BLAST of GR421088 vs. TrEMBL
Match: C6T041_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.882e-11
Identity = 34/55 (61.82%), Postives = 43/55 (78.18%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQNLIL 312
            +IEVDGGLGPSTID+AA A  + +    ++ GAPEP QVISLL+NSVEKAQ  ++
Sbjct:  173 DIEVDGGLGPSTIDVAASAGANCIVAGSSVFGAPEPVQVISLLKNSVEKAQQTLI 227

HSP 2 Score: 22.7126 bits (47), Expect = 1.882e-11
Identity = 9/10 (90.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMPE MDKVR
Sbjct:  154 FMPEMMDKVR 163

[Close]

BLAST of GR421088 vs. TrEMBL
Match: C6T4H4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 4.121e-11
Identity = 35/51 (68.63%), Postives = 42/51 (82.35%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +IEVDGGLGPSTID+AA A  + +    ++ GAPEPAQVISLLR+SVEKAQ
Sbjct:  173 DIEVDGGLGPSTIDVAASAGANCIVAGSSVFGAPEPAQVISLLRSSVEKAQ 223

HSP 2 Score: 21.9422 bits (45), Expect = 4.121e-11
Identity = 9/10 (90.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FM ETMDKVR
Sbjct:  154 FMAETMDKVR 163

[Close]

BLAST of GR421088 vs. TrEMBL
Match: D7UBD8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_171.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001810001 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 3.291e-10
Identity = 33/51 (64.71%), Postives = 40/51 (78.43%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +IEVDGGLGPSTIDMAA A  + +    ++ GAP+P QVISLLR SVE+AQ
Sbjct:  241 DIEVDGGLGPSTIDMAASAGANCIVAGSSVFGAPKPEQVISLLRKSVEEAQ 291

HSP 2 Score: 22.7126 bits (47), Expect = 3.291e-10
Identity = 9/10 (90.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMPE MDKVR
Sbjct:  222 FMPEMMDKVR 231

[Close]

BLAST of GR421088 vs. TrEMBL
Match: A9PIC8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_815572 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.330e-10
Identity = 32/51 (62.75%), Postives = 39/51 (76.47%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +IEVDGGLGPSTIDMAA A  + +    ++ GAP+ AQVISLLR SVE+ Q
Sbjct:  173 DIEVDGGLGPSTIDMAASAGANCIVAGSSVFGAPDSAQVISLLRKSVEEVQ 223

HSP 2 Score: 25.0238 bits (53), Expect = 3.330e-10
Identity = 10/10 (100.00%), Postives = 10/10 (100.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMPETMDKVR
Sbjct:  154 FMPETMDKVR 163

[Close]

BLAST of GR421088 vs. TrEMBL
Match: Q9SGI6_ARATH (Putative D-ribulose-5-phosphate 3-epimerase OS=Arabidopsis thaliana GN=F28J7.18 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.064e-9
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +I+VDGGLGPSTID AA A  + +    ++ GAPEP  VISLLR SVEKAQ
Sbjct:  180 DIQVDGGLGPSTIDTAAAAGANCIVAGSSVFGAPEPGDVISLLRTSVEKAQ 230

HSP 2 Score: 21.557 bits (44), Expect = 2.064e-9
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMP+ MDKVR
Sbjct:  161 FMPDMMDKVR 170

[Close]

BLAST of GR421088 vs. TrEMBL
Match: Q94K13_ARATH (Putative D-ribulose-5-phosphate 3-epimerase OS=Arabidopsis thaliana GN=At3g01850 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.068e-9
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +I+VDGGLGPSTID AA A  + +    ++ GAPEP  VISLLR SVEKAQ
Sbjct:  172 DIQVDGGLGPSTIDTAAAAGANCIVAGSSVFGAPEPGDVISLLRTSVEKAQ 222

HSP 2 Score: 21.557 bits (44), Expect = 2.068e-9
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMP+ MDKVR
Sbjct:  153 FMPDMMDKVR 162

[Close]

BLAST of GR421088 vs. TrEMBL
Match: Q9C8T3_ARATH (At1g63290/F9N12_9 OS=Arabidopsis thaliana GN=At1g63290 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 7.606e-9
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +IEVDGGLGPSTID AA A  + +    ++ GAP+P  VISLLR SVEKAQ
Sbjct:  174 DIEVDGGLGPSTIDAAAAAGANCIVAGSSVFGAPKPGDVISLLRASVEKAQ 224

HSP 2 Score: 20.7866 bits (42), Expect = 7.606e-9
Identity = 8/10 (80.00%), Postives = 8/10 (80.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMP  MDKVR
Sbjct:  155 FMPSMMDKVR 164

[Close]

BLAST of GR421088 vs. TrEMBL
Match: D7KTH9_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_475014 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 7.606e-9
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +IEVDGGLGPSTID AA A  + +    ++ GAPEP  VISLLR SV+KAQ
Sbjct:  174 DIEVDGGLGPSTIDAAAAAGANCIVAGSSVFGAPEPGDVISLLRASVKKAQ 224

HSP 2 Score: 20.7866 bits (42), Expect = 7.606e-9
Identity = 8/10 (80.00%), Postives = 8/10 (80.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMP  MDKVR
Sbjct:  155 FMPNMMDKVR 164

[Close]

BLAST of GR421088 vs. TrEMBL
Match: B9RBK5_RICCO (Ribulose-5-phosphate-3-epimerase, putative OS=Ricinus communis GN=RCOM_1677790 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.340e-8
Identity = 30/51 (58.82%), Postives = 40/51 (78.43%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +IEVDGGLGPSTID+AA A  + +    ++ GAPEPA VISL++ SV++AQ
Sbjct:  173 DIEVDGGLGPSTIDLAASAGANCIVAGSSVFGAPEPAHVISLMKKSVQEAQ 223

[Close]

BLAST of GR421088 vs. TrEMBL
Match: D7KTI8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_893115 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.155e-8
Identity = 31/51 (60.78%), Postives = 37/51 (72.55%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +IEVDGGLGPSTID AA A  + +    ++ GAPEP  VIS LR SV+KAQ
Sbjct:  174 DIEVDGGLGPSTIDAAAAAGANCIVAGSSVFGAPEPGDVISFLRASVKKAQ 224

HSP 2 Score: 20.7866 bits (42), Expect = 2.155e-8
Identity = 8/10 (80.00%), Postives = 8/10 (80.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMP  MDKVR
Sbjct:  155 FMPNMMDKVR 164

[Close]

BLAST of GR421088 vs. TAIR peptide
Match: AT3G01850.2 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:300428-301987 REVERSE LENGTH=225)

HSP 1 Score: 64.3142 bits (155), Expect = 1.623e-11
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +I+VDGGLGPSTID AA A  + +    ++ GAPEP  VISLLR SVEKAQ
Sbjct:  172 DIQVDGGLGPSTIDTAAAAGANCIVAGSSVFGAPEPGDVISLLRTSVEKAQ 222

HSP 2 Score: 21.557 bits (44), Expect = 1.623e-11
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMP+ MDKVR
Sbjct:  153 FMPDMMDKVR 162

[Close]

BLAST of GR421088 vs. TAIR peptide
Match: AT3G01850.1 (| Symbols: | Aldolase-type TIM barrel family protein | chr3:300428-301987 REVERSE LENGTH=225)

HSP 1 Score: 64.3142 bits (155), Expect = 1.623e-11
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +I+VDGGLGPSTID AA A  + +    ++ GAPEP  VISLLR SVEKAQ
Sbjct:  172 DIQVDGGLGPSTIDTAAAAGANCIVAGSSVFGAPEPGDVISLLRTSVEKAQ 222

HSP 2 Score: 21.557 bits (44), Expect = 1.623e-11
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMP+ MDKVR
Sbjct:  153 FMPDMMDKVR 162

[Close]

BLAST of GR421088 vs. TAIR peptide
Match: AT1G63290.1 (| Symbols: | Aldolase-type TIM barrel family protein | chr1:23472095-23473590 REVERSE LENGTH=227)

HSP 1 Score: 63.1586 bits (152), Expect = 5.968e-11
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1

Query:  148 NIEVDGGLGPSTIDMAALAAGSSVFEQIALLGAPEPAQVISLLRNSVEKAQ 300
            +IEVDGGLGPSTID AA A  + +    ++ GAP+P  VISLLR SVEKAQ
Sbjct:  174 DIEVDGGLGPSTIDAAAAAGANCIVAGSSVFGAPKPGDVISLLRASVEKAQ 224

HSP 2 Score: 20.7866 bits (42), Expect = 5.968e-11
Identity = 8/10 (80.00%), Postives = 8/10 (80.00%), Query Frame = 3

Query:   72 FMPETMDKVR 101
            FMP  MDKVR
Sbjct:  155 FMPSMMDKVR 164

The following BLAST results are available for this feature:

BLAST of GR421088 vs. SwissProt
BLAST of GR421088 vs. TrEMBL
BLAST of GR421088 vs. TAIR peptide

BLAST of GR421088 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
RPE1_ORYSJ	2.659e-9	57.69	Ribulose-phosphate 3-epimerase, cytoplasmic isofor...	[more]

BLAST of GR421088 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6T041_SOYBN	1.882e-11	61.82	Putative uncharacterized protein (Fragment) OS=Gly...	[more]
C6T4H4_SOYBN	4.121e-11	68.63	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7UBD8_VITVI	3.291e-10	64.71	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9PIC8_POPTR	3.330e-10	62.75	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9SGI6_ARATH	2.064e-9	62.75	Putative D-ribulose-5-phosphate 3-epimerase OS=Ara...	[more]
Q94K13_ARATH	2.068e-9	62.75	Putative D-ribulose-5-phosphate 3-epimerase OS=Ara...	[more]
Q9C8T3_ARATH	7.606e-9	62.75	At1g63290/F9N12_9 OS=Arabidopsis thaliana GN=At1g6...	[more]
D7KTH9_ARALY	7.606e-9	62.75	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9RBK5_RICCO	1.340e-8	58.82	Ribulose-5-phosphate-3-epimerase, putative OS=Rici...	[more]
D7KTI8_ARALY	2.155e-8	60.78	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of GR421088 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT3G01850.2	1.623e-11	62.75	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT3G01850.1	1.623e-11	62.75	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]
AT1G63290.1	5.968e-11	62.75	\| Symbols: \| Aldolase-type TIM barrel family prot...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000056	Ribulose-phosphate 3-epimerase	PANTHER	PTHR11749	RIBULOSE-5-PHOSPHATE-3-EPIMERASE	coord: 2..58 score: 3.4
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	no description	coord: 2..53 score: 2.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR421088 ID=GR421088; Name=GR421088; organism=Cicer arietinum; type=EST; length=549bp
TGAGTAGACGCAGCTGTGGTAGCGGTTTATCAAACTGTTCTTGTGATCCT
GTGGAAATGGACTGTAGAACCTTTATGCCAGAGACGATGGACAAGGTACG
GGGATTCGGAGGACAAAAGTGAAGTATCCAGCACTTGATACTGAGGAAAC
ATAGAGGTTGATGGTGGTTTAGGGCCTTCAACCATAGACATGGCTGCATT
AGCGGCCGGAAGTTCAGTATTTGAGCAAATTGCATTGTTGGGAGCCCCTG
AGCCAGCTCAAGTAATATCCTTACTGAGAAATTCTGTTGAGAAAGCCCAG
AACTTGATATTAACATATTTAATAAAGATCTTTCATTGATGTTACAATGA
TTGTAACTTCGCAATTTCATGTAATCTATATGCTTGTTGTGCAGAGATCA
AGTGATTTAAAGTCAAATATTCTAATAATGTCACTGATGTCTCCAAAACT
GTAATACATCAATATTTAATGTGTACTTCAGGCATAAAAGGAGTGACACA
TATTGCTGTCACCTGATTAAACATTTACAAATAGCTTTATTTTTGCTAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004750	D-ribulose-phosphate 3-epimerase activity
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000056	Ribul_P_3_epim-like
IPR013785	Aldolase_TIM

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