GR916746

EST Overview


Name	GR916746
Unique Name	GR916746
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	183

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_ARATH (T-complex protein 1 subunit alpha OS=Arabidopsis thaliana GN=CCT1 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 6.077e-21
Identity = 48/54 (88.89%), Postives = 53/54 (98.15%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKEDMRHVAKATGATLV+TFADMEGEETF+P+ LG+ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDMRHVAKATGATLVTTFADMEGEETFDPAHLGSADEV 354

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_MOUSE (T-complex protein 1 subunit alpha OS=Mus musculus GN=Tcp1 PE=1 SV=3)

HSP 1 Score: 77.7962 bits (190), Expect = 1.893e-14
Identity = 36/54 (66.67%), Postives = 48/54 (88.89%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGA+AVRRV K D++HVAKA+GA+++ST A++EGEETFE + LG A+EV
Sbjct:  298 KYFVEAGAMAVRRVLKRDLKHVAKASGASILSTLANLEGEETFEVTMLGQAEEV 351

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_BOVIN (T-complex protein 1 subunit alpha OS=Bos taurus GN=TCP1 PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 3.230e-14
Identity = 36/54 (66.67%), Postives = 47/54 (87.04%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGA+AVRRV K D++ +AKA+GAT++ST A++EGEETFE S LG A+EV
Sbjct:  298 KYFVEAGAMAVRRVLKRDLKRIAKASGATVLSTLANLEGEETFEASMLGQAEEV 351

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_MACFA (T-complex protein 1 subunit alpha OS=Macaca fascicularis GN=TCP1 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 5.509e-14
Identity = 35/54 (64.81%), Postives = 47/54 (87.04%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGA+AVRRV K D++ +AKA+GAT++ST A++EGEETFE + LG A+EV
Sbjct:  298 KYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEV 351

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_HUMAN (T-complex protein 1 subunit alpha OS=Homo sapiens GN=TCP1 PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 5.509e-14
Identity = 35/54 (64.81%), Postives = 47/54 (87.04%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGA+AVRRV K D++ +AKA+GAT++ST A++EGEETFE + LG A+EV
Sbjct:  298 KYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEV 351

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_RAT (T-complex protein 1 subunit alpha OS=Rattus norvegicus GN=Tcp1 PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.603e-13
Identity = 34/54 (62.96%), Postives = 47/54 (87.04%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGA+AVRRV K D++ +AKA+GA+++ST A++EGEETFE + LG A+EV
Sbjct:  298 KYFVEAGAMAVRRVLKRDLKRIAKASGASILSTLANLEGEETFEATMLGQAEEV 351

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_CRIGR (T-complex protein 1 subunit alpha OS=Cricetulus griseus GN=TCP1 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.603e-13
Identity = 34/54 (62.96%), Postives = 47/54 (87.04%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGA+AVRRV K D++ +AKA+GA+++ST A++EGEETFE + LG A+EV
Sbjct:  298 KYFVEAGAMAVRRVLKRDLKRIAKASGASILSTLANLEGEETFEATMLGQAEEV 351

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_SCHPO (T-complex protein 1 subunit alpha OS=Schizosaccharomyces pombe GN=cct1 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.734e-13
Identity = 33/51 (64.71%), Postives = 47/51 (92.16%), Query Frame = 1

Query:   31 VEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +EAGA+AVRR +KED+R +AKA+GATL+S+ +++EGEETFE S+LG+A+EV
Sbjct:  306 IEAGAMAVRRCKKEDLRRIAKASGATLLSSLSNLEGEETFESSYLGSAEEV 356

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_PALPA (T-complex protein 1 subunit alpha OS=Paleosuchus palpebrosus GN=TCP1 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.734e-13
Identity = 34/54 (62.96%), Postives = 47/54 (87.04%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFV+AGA+AVRRV K+D++ +AKA+GAT+ ST A++EGEE+FE S LG A+EV
Sbjct:  301 KYFVDAGAMAVRRVLKKDLKRIAKASGATVCSTLANLEGEESFEASMLGQAEEV 354

[Close]

BLAST of GR916746 vs. SwissProt
Match: TCPA_YEAST (T-complex protein 1 subunit alpha OS=Saccharomyces cerevisiae GN=TCP1 PE=1 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 2.315e-12
Identity = 33/52 (63.46%), Postives = 43/52 (82.69%), Query Frame = 1

Query:   28 FVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            FVEA  + VRR +KED+R +A+ATGATLVS+ +++EGEETFE S+LG  DEV
Sbjct:  310 FVEAKIMGVRRCKKEDLRRIARATGATLVSSMSNLEGEETFESSYLGLCDEV 361

[Close]

BLAST of GR916746 vs. TrEMBL
Match: B9MUZ2_POPTR (T-complex protein 1 subunit alpha OS=Populus trichocarpa GN=POPTRDRAFT_836390 PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.978e-21
Identity = 52/54 (96.30%), Postives = 53/54 (98.15%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLG ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGYADEV 354

[Close]

BLAST of GR916746 vs. TrEMBL
Match: Q8H0U0_ARATH (T-complex protein 1 subunit alpha OS=Arabidopsis thaliana GN=At3g20050 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.280e-19
Identity = 48/54 (88.89%), Postives = 53/54 (98.15%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKEDMRHVAKATGATLV+TFADMEGEETF+P+ LG+ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDMRHVAKATGATLVTTFADMEGEETFDPAHLGSADEV 354

[Close]

BLAST of GR916746 vs. TrEMBL
Match: Q5E917_ARATH (T-complex protein 1 subunit alpha OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.280e-19
Identity = 48/54 (88.89%), Postives = 53/54 (98.15%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKEDMRHVAKATGATLV+TFADMEGEETF+P+ LG+ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDMRHVAKATGATLVTTFADMEGEETFDPAHLGSADEV 354

[Close]

BLAST of GR916746 vs. TrEMBL
Match: D7LAV8_ARALY (T-complex protein alpha subunit of chaperonin OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_479524 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.280e-19
Identity = 48/54 (88.89%), Postives = 53/54 (98.15%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKEDMRHVAKATGATLV+TFADMEGEETF+P+ LG+ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDMRHVAKATGATLVTTFADMEGEETFDPAHLGSADEV 354

[Close]

BLAST of GR916746 vs. TrEMBL
Match: B9GPB0_POPTR (T-complex protein 1 subunit alpha OS=Populus trichocarpa GN=POPTRDRAFT_816547 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.671e-19
Identity = 50/54 (92.59%), Postives = 51/54 (94.44%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFE S LG ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFESSLLGYADEV 354

[Close]

BLAST of GR916746 vs. TrEMBL
Match: Q7FAT6_ORYSJ (T-complex protein 1 subunit alpha OS=Oryza sativa subsp. japonica GN=OSJNBa0010H02.6 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.851e-19
Identity = 48/54 (88.89%), Postives = 52/54 (96.30%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKED+RHVAKATGAT+VSTFADMEGEETF+ SFLG ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDLRHVAKATGATMVSTFADMEGEETFDSSFLGHADEV 354

[Close]

BLAST of GR916746 vs. TrEMBL
Match: C5XUD0_SORBI (T-complex protein 1 subunit alpha OS=Sorghum bicolor GN=Sb04g035610 PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.851e-19
Identity = 48/54 (88.89%), Postives = 52/54 (96.30%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKED+RHVAKATGAT+VSTFADMEGEETF+ SFLG ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDLRHVAKATGATMVSTFADMEGEETFDASFLGHADEV 354

[Close]

BLAST of GR916746 vs. TrEMBL
Match: B8ASU6_ORYSI (T-complex protein 1 subunit alpha OS=Oryza sativa subsp. indica GN=OsI_16908 PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.851e-19
Identity = 48/54 (88.89%), Postives = 52/54 (96.30%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKED+RHVAKATGAT+VSTFADMEGEETF+ SFLG ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDLRHVAKATGATMVSTFADMEGEETFDSSFLGHADEV 354

[Close]

BLAST of GR916746 vs. TrEMBL
Match: B6TKY3_MAIZE (T-complex protein 1 subunit alpha OS=Zea mays PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.851e-19
Identity = 48/54 (88.89%), Postives = 52/54 (96.30%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKED+RHVAKATGAT+VSTFADMEGEETF+ SFLG ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDLRHVAKATGATMVSTFADMEGEETFDSSFLGHADEV 354

[Close]

BLAST of GR916746 vs. TrEMBL
Match: B9SF60_RICCO (T-complex protein 1 subunit alpha OS=Ricinus communis GN=RCOM_1276510 PE=3 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.862e-19
Identity = 47/54 (87.04%), Postives = 51/54 (94.44%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKEDMRHVAKATG T+VSTFADMEG+ETF+PS LG ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDMRHVAKATGGTMVSTFADMEGDETFDPSLLGYADEV 354

[Close]

BLAST of GR916746 vs. TAIR peptide
Match: AT3G20050.1 (| Symbols: ATTCP-1, TCP-1 | T-complex protein 1 alpha subunit | chr3:6998544-7002266 REVERSE LENGTH=545)

HSP 1 Score: 99.3673 bits (246), Expect = 4.873e-22
Identity = 48/54 (88.89%), Postives = 53/54 (98.15%), Query Frame = 1

Query:   22 RYFVEAGAIAVRRVRKEDMRHVAKATGATLVSTFADMEGEETFEPSFLGTADEV 183
            +YFVEAGAIAVRRVRKEDMRHVAKATGATLV+TFADMEGEETF+P+ LG+ADEV
Sbjct:  301 KYFVEAGAIAVRRVRKEDMRHVAKATGATLVTTFADMEGEETFDPAHLGSADEV 354

The following BLAST results are available for this feature:

BLAST of GR916746 vs. SwissProt
BLAST of GR916746 vs. TrEMBL
BLAST of GR916746 vs. TAIR peptide

BLAST of GR916746 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TCPA_ARATH	6.077e-21	88.89	T-complex protein 1 subunit alpha OS=Arabidopsis t...	[more]
TCPA_MOUSE	1.893e-14	66.67	T-complex protein 1 subunit alpha OS=Mus musculus ...	[more]
TCPA_BOVIN	3.230e-14	66.67	T-complex protein 1 subunit alpha OS=Bos taurus GN...	[more]
TCPA_MACFA	5.509e-14	64.81	T-complex protein 1 subunit alpha OS=Macaca fascic...	[more]
TCPA_HUMAN	5.509e-14	64.81	T-complex protein 1 subunit alpha OS=Homo sapiens ...	[more]
TCPA_RAT	1.603e-13	62.96	T-complex protein 1 subunit alpha OS=Rattus norveg...	[more]
TCPA_CRIGR	1.603e-13	62.96	T-complex protein 1 subunit alpha OS=Cricetulus gr...	[more]
TCPA_SCHPO	2.734e-13	64.71	T-complex protein 1 subunit alpha OS=Schizosacchar...	[more]
TCPA_PALPA	2.734e-13	62.96	T-complex protein 1 subunit alpha OS=Paleosuchus p...	[more]
TCPA_YEAST	2.315e-12	63.46	T-complex protein 1 subunit alpha OS=Saccharomyces...	[more]

BLAST of GR916746 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9MUZ2_POPTR	3.978e-21	96.30	T-complex protein 1 subunit alpha OS=Populus trich...	[more]
Q8H0U0_ARATH	1.280e-19	88.89	T-complex protein 1 subunit alpha OS=Arabidopsis t...	[more]
Q5E917_ARATH	1.280e-19	88.89	T-complex protein 1 subunit alpha OS=Arabidopsis t...	[more]
D7LAV8_ARALY	1.280e-19	88.89	T-complex protein alpha subunit of chaperonin OS=A...	[more]
B9GPB0_POPTR	1.671e-19	92.59	T-complex protein 1 subunit alpha OS=Populus trich...	[more]
Q7FAT6_ORYSJ	2.851e-19	88.89	T-complex protein 1 subunit alpha OS=Oryza sativa ...	[more]
C5XUD0_SORBI	2.851e-19	88.89	T-complex protein 1 subunit alpha OS=Sorghum bicol...	[more]
B8ASU6_ORYSI	2.851e-19	88.89	T-complex protein 1 subunit alpha OS=Oryza sativa ...	[more]
B6TKY3_MAIZE	2.851e-19	88.89	T-complex protein 1 subunit alpha OS=Zea mays PE=2...	[more]
B9SF60_RICCO	4.862e-19	87.04	T-complex protein 1 subunit alpha OS=Ricinus commu...	[more]

BLAST of GR916746 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G20050.1	4.873e-22	88.89	\| Symbols: ATTCP-1, TCP-1 \| T-complex protein 1 al...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002423	Chaperonin Cpn60/TCP-1	PANTHER	PTHR11353	CHAPERONIN	coord: 8..61 score: 1.7
IPR002423	Chaperonin Cpn60/TCP-1	PFAM	PF00118	Cpn60_TCP1	coord: 8..61 score: 6
IPR012715	T-complex protein 1, alpha subunit	PANTHER	PTHR11353:SF20	CHAPERONIN CONTAINING T-COMPLEX PROTEIN 1, ALPHA SUBUNIT, TCPA	coord: 8..61 score: 1.7
None	No IPR available	GENE3D	3.50.7.10	no description	coord: 8..61 score: 2
None	No IPR available	SUPERFAMILY	52029	GroEL apical domain-like	coord: 9..61 score: 5.2
None	No IPR available	SEG	seg	seg	coord: 14..32 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR916746 ID=GR916746; Name=GR916746; organism=Cicer arietinum; type=EST; length=183bp
GATTTCGAGCGGCGCCCGGGCAGGTACTTTGTTGAGGCTGGAGCAATTGC
TGTGAGACGTGTGCGGAAAGAGGATATGCGTCATGTTGCCAAAGCAACTG
GGGCAACCTTGGTCTCAACATTTGCTGACATGGAAGGGGAGGAAACTTTT
GAACCATCATTTCTTGGAACTGCAGATGAGGTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0051082	unfolded protein binding

Vocabulary: **Biological Process**
Term	Definition
GO:0044267	cellular protein metabolic process
GO:0006457	protein folding

Vocabulary: **INTERPRO**
Term	Definition
IPR002423	Cpn60/GroEL/TCP-1
IPR012715	Chap_CCT_alpha

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