HO066706

EST Overview
NameHO066706
Unique NameHO066706
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length216
Libraries
Library NameType
LIBEST_026413 Chickpea drought stressed cDNA SSH library AS2cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
Homology
[Close]
BLAST of HO066706 vs. SwissProt
Match: 4CL1_SOYBN (4-coumarate--CoA ligase 1 (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.198e-8
Identity = 28/33 (84.85%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQVVFYKR NRVFF DAI  SPSGKILRK
Sbjct:  249 QFISKQVVFYKRINRVFFIDAIPKSPSGKILRK 281
[Close]
BLAST of HO066706 vs. SwissProt
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.014e-7
Identity = 25/33 (75.76%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQV+FYKR NRVFF +AI  +PSGKILRK
Sbjct:  507 QFISKQVIFYKRINRVFFVEAIPKAPSGKILRK 539
[Close]
BLAST of HO066706 vs. SwissProt
Match: 4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL2 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.014e-7
Identity = 28/33 (84.85%), Postives = 28/33 (84.85%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QFVSKQVVFYKR  RVFF DAI  SPSGKILRK
Sbjct:  499 QFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRK 531
[Close]
BLAST of HO066706 vs. SwissProt
Match: 4CL2_ARATH (4-coumarate--CoA ligase 2 OS=Arabidopsis thaliana GN=4CL2 PE=1 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 1.014e-7
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QFVSKQVVFYKR N+VFF D+I  +PSGKILRK
Sbjct:  512 QFVSKQVVFYKRINKVFFTDSIPKAPSGKILRK 544
[Close]
BLAST of HO066706 vs. SwissProt
Match: 4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.014e-7
Identity = 28/33 (84.85%), Postives = 28/33 (84.85%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QFVSKQVVFYKR  RVFF DAI  SPSGKILRK
Sbjct:  499 QFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRK 531
[Close]
BLAST of HO066706 vs. SwissProt
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.782e-7
Identity = 25/32 (78.12%), Postives = 27/32 (84.38%), Query Frame = -2 
Query:  117 FVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 212
            F+SKQV+FYKR  RVFF DAI  SPSGKILRK
Sbjct:  499 FISKQVIFYKRIKRVFFVDAIPKSPSGKILRK 530

HSP 2 Score: 20.4014 bits (41), Expect = 1.782e-7
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = -1 
Query:   79 AKLAAGVPN 105
            AKLAAG+PN
Sbjct:  534 AKLAAGLPN 542
[Close]
BLAST of HO066706 vs. SwissProt
Match: 4CL4_ORYSJ (Probable 4-coumarate--CoA ligase 4 OS=Oryza sativa subsp. japonica GN=4CL4 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.259e-7
Identity = 25/33 (75.76%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QFV+K+VVFYKR N+VFF D+I  SPSGKILRK
Sbjct:  507 QFVAKEVVFYKRLNKVFFADSIPKSPSGKILRK 539
[Close]
BLAST of HO066706 vs. SwissProt
Match: 4CL1_ARATH (4-coumarate--CoA ligase 1 OS=Arabidopsis thaliana GN=4CL1 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.951e-7
Identity = 25/33 (75.76%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QFVSKQVVFYKR N+VFF ++I  +PSGKILRK
Sbjct:  519 QFVSKQVVFYKRINKVFFTESIPKAPSGKILRK 551
[Close]
BLAST of HO066706 vs. TrEMBL
Match: D2K6F7_9FABA (4-coumarate:CoA ligase OS=Galega orientalis GN=4CL PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.612e-8
Identity = 28/33 (84.85%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQVVFYKR NRVFF DAI  SPSGKILRK
Sbjct:  506 QFISKQVVFYKRINRVFFIDAIPKSPSGKILRK 538

HSP 2 Score: 21.557 bits (44), Expect = 7.612e-8
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -1 
Query:   79 AKLAAGVPN 105
            AKLAAGVPN
Sbjct:  542 AKLAAGVPN 550
[Close]
BLAST of HO066706 vs. TrEMBL
Match: B8XCX9_9LAMI (4-coumarate coenzyme A ligase OS=Paulownia fortunei GN=4CL PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.660e-7
Identity = 28/33 (84.85%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQVVFYKR NRVFF DAI  SPSGKILRK
Sbjct:  499 QFISKQVVFYKRINRVFFIDAIPKSPSGKILRK 531

HSP 2 Score: 20.4014 bits (41), Expect = 1.660e-7
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = -1 
Query:   79 AKLAAGVPN 105
            A+LAAGVPN
Sbjct:  535 ARLAAGVPN 543
[Close]
BLAST of HO066706 vs. TrEMBL
Match: Q8S5C1_SOYBN (4-coumarate:CoA ligase isoenzyme 2 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.512e-7
Identity = 28/33 (84.85%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQVVFYKR NRVFF DAI  SPSGKILRK
Sbjct:  503 QFISKQVVFYKRINRVFFIDAIPKSPSGKILRK 535
[Close]
BLAST of HO066706 vs. TrEMBL
Match: Q8W558_9FABA (4-coumarate:CoA ligase OS=Amorpha fruticosa GN=4CL PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.791e-7
Identity = 27/33 (81.82%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQVVFYKR +RVFF DAI  SPSGKILRK
Sbjct:  496 QFISKQVVFYKRISRVFFIDAIPKSPSGKILRK 528

HSP 2 Score: 21.557 bits (44), Expect = 2.791e-7
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -1 
Query:   79 AKLAAGVPN 105
            AKLAAGVPN
Sbjct:  532 AKLAAGVPN 540
[Close]
BLAST of HO066706 vs. TrEMBL
Match: Q67G17_SALMI (4-coumarate:coenzyme A ligase 2 OS=Salvia miltiorrhiza PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 3.280e-7
Identity = 27/33 (81.82%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQV+FYKR NRVFF DAI  SPSGKILRK
Sbjct:  498 QFISKQVIFYKRINRVFFIDAIPKSPSGKILRK 530
[Close]
BLAST of HO066706 vs. TrEMBL
Match: Q0GZS1_9BORA (4-coumarate:CoA ligase OS=Arnebia euchroma PE=2 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 5.596e-7
Identity = 27/33 (81.82%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QFVSKQV+FYKR NRVFF D+I  SPSGKILRK
Sbjct:  501 QFVSKQVIFYKRINRVFFVDSIPKSPSGKILRK 533
[Close]
BLAST of HO066706 vs. TrEMBL
Match: D5LLN7_SORAU (p-coumarate:CoA ligase 1 OS=Sorbus aucuparia PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 7.308e-7
Identity = 27/33 (81.82%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQVVFYKR NRVFF +AI  SPSGKILRK
Sbjct:  503 QFISKQVVFYKRINRVFFIEAIPKSPSGKILRK 535
[Close]
BLAST of HO066706 vs. TrEMBL
Match: C0KKW5_HUMLU (4-coumarate:CoA ligase OS=Humulus lupulus PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 7.308e-7
Identity = 27/33 (81.82%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQVVFYKR NRVFF +AI  SPSGKILRK
Sbjct:  505 QFISKQVVFYKRINRVFFIEAIPKSPSGKILRK 537
[Close]
BLAST of HO066706 vs. TrEMBL
Match: B3FES3_9ROSA (4-coumarate coenzyme A ligase (Fragment) OS=Eriobotrya japonica PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 7.308e-7
Identity = 27/33 (81.82%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QF+SKQVVFYKR NRVFF +AI  SPSGKILRK
Sbjct:  302 QFISKQVVFYKRINRVFFIEAIPKSPSGKILRK 334
[Close]
BLAST of HO066706 vs. TrEMBL
Match: Q2YHM7_PLAMJ (4-coumaryl-CoA ligase (Fragment) OS=Plantago major GN=4CL2 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.545e-7
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            +F+SKQV+FYKR NRVFF DAI  SPSGKILRK
Sbjct:   79 KFISKQVIFYKRINRVFFIDAIPKSPSGKILRK 111
[Close]
BLAST of HO066706 vs. TAIR peptide
Match: AT3G21240.1 (| Symbols: 4CL2, AT4CL2 | 4-coumarate:CoA ligase 2 | chr3:7454497-7457314 REVERSE LENGTH=556)

HSP 1 Score: 55.4546 bits (132), Expect = 8.167e-9
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QFVSKQVVFYKR N+VFF D+I  +PSGKILRK
Sbjct:  512 QFVSKQVVFYKRINKVFFTDSIPKAPSGKILRK 544
[Close]
BLAST of HO066706 vs. TAIR peptide
Match: AT1G51680.1 (| Symbols: 4CL1, 4CL.1, AT4CL1 | 4-coumarate:CoA ligase 1 | chr1:19159007-19161464 REVERSE LENGTH=561)

HSP 1 Score: 53.9138 bits (128), Expect = 2.376e-8
Identity = 25/33 (75.76%), Postives = 29/33 (87.88%), Query Frame = -2 
Query:  117 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRK 215
            QFVSKQVVFYKR N+VFF ++I  +PSGKILRK
Sbjct:  519 QFVSKQVVFYKRINKVFFTESIPKAPSGKILRK 551
[Close]
BLAST of HO066706 vs. TAIR peptide
Match: AT1G65060.1 (| Symbols: 4CL3 | 4-coumarate:CoA ligase 3 | chr1:24167385-24171457 REVERSE LENGTH=561)

HSP 1 Score: 48.521 bits (114), Expect = 9.984e-7
Identity = 23/36 (63.89%), Postives = 29/36 (80.56%), Query Frame = -2 
Query:  108 QFVSKQVVFYKRXNRVFFXDAIXXSPSGKILRKGPK 215
            ++V+KQVVFYKR ++VFF  +I  SPSGKILRK  K
Sbjct:  522 EYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLK 557
The following BLAST results are available for this feature:
BLAST of HO066706 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 8
Match NameE-valueIdentityDescription
4CL1_SOYBN1.198e-884.854-coumarate--CoA ligase 1 (Fragment) OS=Glycine ma... [more]
4CL_VANPL1.014e-775.764-coumarate--CoA ligase OS=Vanilla planifolia GN=4... [more]
4CL2_PETCR1.014e-784.854-coumarate--CoA ligase 1 OS=Petroselinum crispum ... [more]
4CL2_ARATH1.014e-778.794-coumarate--CoA ligase 2 OS=Arabidopsis thaliana ... [more]
4CL1_PETCR1.014e-784.854-coumarate--CoA ligase 1 OS=Petroselinum crispum ... [more]
4CL2_TOBAC1.782e-778.134-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=... [more]
4CL4_ORYSJ2.259e-775.76Probable 4-coumarate--CoA ligase 4 OS=Oryza sativa... [more]
4CL1_ARATH2.951e-775.764-coumarate--CoA ligase 1 OS=Arabidopsis thaliana ... [more]
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BLAST of HO066706 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D2K6F7_9FABA7.612e-884.854-coumarate:CoA ligase OS=Galega orientalis GN=4CL... [more]
B8XCX9_9LAMI1.660e-784.854-coumarate coenzyme A ligase OS=Paulownia fortune... [more]
Q8S5C1_SOYBN2.512e-784.854-coumarate:CoA ligase isoenzyme 2 OS=Glycine max ... [more]
Q8W558_9FABA2.791e-781.824-coumarate:CoA ligase OS=Amorpha fruticosa GN=4CL... [more]
Q67G17_SALMI3.280e-781.824-coumarate:coenzyme A ligase 2 OS=Salvia miltiorr... [more]
Q0GZS1_9BORA5.596e-781.824-coumarate:CoA ligase OS=Arnebia euchroma PE=2 SV... [more]
D5LLN7_SORAU7.308e-781.82p-coumarate:CoA ligase 1 OS=Sorbus aucuparia PE=2 ... [more]
C0KKW5_HUMLU7.308e-781.824-coumarate:CoA ligase OS=Humulus lupulus PE=2 SV=... [more]
B3FES3_9ROSA7.308e-781.824-coumarate coenzyme A ligase (Fragment) OS=Eriobo... [more]
Q2YHM7_PLAMJ9.545e-778.794-coumaryl-CoA ligase (Fragment) OS=Plantago major... [more]
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BLAST of HO066706 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT3G21240.18.167e-978.79| Symbols: 4CL2, AT4CL2 | 4-coumarate:CoA ligase 2... [more]
AT1G51680.12.376e-875.76| Symbols: 4CL1, 4CL.1, AT4CL1 | 4-coumarate:CoA l... [more]
AT1G65060.19.984e-763.89| Symbols: 4CL3 | 4-coumarate:CoA ligase 3 | chr1:... [more]
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Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO066706 ID=HO066706; Name=HO066706; organism=Cicer arietinum; type=EST; length=216bp
CCCTTTATGAATACNTGCTTTCTTTNAAGGGATGAATACCTGCTATATAT
GATTAAATGTAAATTGATTGATTATTCAATTTGGAACACCAGCTGCAAGC
TTAGCACCTTAGGTCCCTTTCGTAATATTTTGCCTGAGGGTGACNNANGA
ATTGCATCANNGAAGAACACTCTGTTTANTCTTTTGTAAAANACCACCTG
TTTGGAGACGAACTGT
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