GR409708

EST Overview


Name	GR409708
Unique Name	GR409708
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	474

Libraries


Library Name	Type
LIBEST_024779 ICC1882 Slow drought stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_DICDI (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Dictyostelium discoideum GN=pdhB PE=1 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 2.679e-46
Identity = 86/141 (60.99%), Postives = 115/141 (81.56%), Query Frame = -3

Query:   50 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYRSK 472
            ++LVPIGKAK+ER+G DVT++  SR+V   ++AA+ L  EGIS EVINLRTIRP+D +TI+ S+KKT ++VT EEG+ Q G+GAEI+AL+MEH FDYLDAP+ER+  ADVPMPYA ++E+ A+ Q  N++NA KRVT R+K
Sbjct:  216 EYLVPIGKAKVEREGKDVTIVGFSRIVSNCMEAAEILAKEGISAEVINLRTIRPIDAETIVNSLKKTNKLVTVEEGWAQSGIGAEISALMMEHAFDYLDAPIERICGADVPMPYASNLENAAMVQTQNIVNAAKRVTQRNK 356

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_ARATH (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Arabidopsis thaliana GN=PDH2 PE=1 SV=2)

HSP 1 Score: 176.407 bits (446), Expect = 5.585e-44
Identity = 90/138 (65.22%), Postives = 110/138 (79.71%), Query Frame = -3

Query:   50 VPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYRSK 463
            +PIGKAKIER+G DVT++T S+MVG A+KAA++L  EGIS EVINLR+IRPLD  TI ASV+KT R+VT EEGF Q GV AEI A ++E  F YLDAPVER+A ADVPMPYA ++E LA+PQ  +++ A KR  YRSK
Sbjct:  226 LPIGKAKIEREGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVCAEICASVVEESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIVRASKRACYRSK 363

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_PEA (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 3.746e-40
Identity = 85/139 (61.15%), Postives = 104/139 (74.82%), Query Frame = -3

Query:   53 WLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYRS 469
            WL PIGKAKIER+G DVT+   S+MVG A+KAA+ L+ EGIS EVINLR+IRPLD  TI ASV+KT R+VT EEGF Q GVGAEI   ++E  F YLDA VER+  ADVPMPYA ++E L +P   +++ A KR  +RS
Sbjct:  215 WL-PIGKAKIEREGKDVTITAFSKMVGFALKAAEILEKEGISAEVINLRSIRPLDRPTINASVRKTNRLVTVEEGFPQHGVGAEICTSVIEESFGYLDATVERIGGADVPMPYAGNLERLVVPHVEDIVRAAKRACHRS 352

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_PONAB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Pongo abelii GN=PDHB PE=2 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 2.685e-38
Identity = 81/136 (59.56%), Postives = 104/136 (76.47%), Query Frame = -3

Query:   68 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLME-HCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKR 472
            D+L+PIGKAKIERQG+ +TV++HSR VG  ++AA  L  EG+  EVIN+RTIRP+D++TI ASV KT  +VT E G+ Q GVGAEI A +ME   F++LDAP  RV  ADVPMPYAK +ED +IPQ  ++I AIK+
Sbjct:  220 DFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKK 355

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_HUMAN (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Homo sapiens GN=PDHB PE=1 SV=3)

HSP 1 Score: 157.532 bits (397), Expect = 2.685e-38
Identity = 81/136 (59.56%), Postives = 104/136 (76.47%), Query Frame = -3

Query:   68 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLME-HCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKR 472
            D+L+PIGKAKIERQG+ +TV++HSR VG  ++AA  L  EG+  EVIN+RTIRP+D++TI ASV KT  +VT E G+ Q GVGAEI A +ME   F++LDAP  RV  ADVPMPYAK +ED +IPQ  ++I AIK+
Sbjct:  220 DFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKK 355

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_BOVIN (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Bos taurus GN=PDHB PE=1 SV=2)

HSP 1 Score: 156.762 bits (395), Expect = 4.580e-38
Identity = 81/136 (59.56%), Postives = 102/136 (75.00%), Query Frame = -3

Query:   68 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLME-HCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKR 472
            D+L+PIGKAKIERQG+ VT++ HSR VG  ++AA  L  EGI  EVINLRTIRP+D++TI  SV KT  +VT E G+ Q GVGAEI A +ME   F++LDAP  RV  ADVPMPYAK +ED ++PQ  ++I AIK+
Sbjct:  220 DFLIPIGKAKIERQGTHVTIVAHSRPVGHCLEAATVLSKEGIECEVINLRTIRPMDIETIEGSVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSVPQVKDIIFAIKK 355

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_RAT (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Rattus norvegicus GN=Pdhb PE=1 SV=2)

HSP 1 Score: 156.377 bits (394), Expect = 5.981e-38
Identity = 82/136 (60.29%), Postives = 102/136 (75.00%), Query Frame = -3

Query:   68 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLME-HCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKR 472
            D+L+PIGKAKIERQG+ +TV+ HSR VG  ++AA  L  EGI  EVINLRTIRP+D++ I ASV KT  +VT E G+ Q GVGAEI A +ME   F++LDAP  RV  ADVPMPYAK +ED +IPQ  ++I AIK+
Sbjct:  220 DFLIPIGKAKIERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDIEAIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKK 355

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_RHIME (Pyruvate dehydrogenase E1 component subunit beta OS=Rhizobium meliloti GN=pdhB PE=3 SV=2)

HSP 1 Score: 155.606 bits (392), Expect = 1.020e-37
Identity = 75/139 (53.96%), Postives = 103/139 (74.10%), Query Frame = -3

Query:   56 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYR 472
            D+++PIGKA+I R G D T+++    +  AIKAA +L+ +GI VE+I+LRTIRP+DL T+I SVKKTGR+VT EEG+ Q  VG EIA  +M+  FDYLDAP+  +A  DVPMPYA ++E LA+P    V++A+K V Y+
Sbjct:  322 DFVLPIGKARIHRTGKDATLVSFGIGMTYAIKAAAELEAQGIDVEIIDLRTIRPMDLPTVIESVKKTGRLVTVEEGYPQSSVGTEIATRVMQQAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVAEVVDAVKAVCYK 460

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_MOUSE (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Mus musculus GN=Pdhb PE=1 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 1.020e-37
Identity = 80/136 (58.82%), Postives = 102/136 (75.00%), Query Frame = -3

Query:   68 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLME-HCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKR 472
            D+L+PIGKAKIERQG+ +TV+ HSR VG  ++AA  L  EGI  EVINLRTIRP+D++ I ASV KT  +VT E G+ Q GVGAEI A +ME   F++LDAP  RV  ADVPMPYAK +ED ++PQ  ++I A+K+
Sbjct:  220 DFLIPIGKAKIERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDIEAIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKVLEDNSVPQVKDIIFAVKK 355

[Close]

BLAST of GR409708 vs. SwissProt
Match: ODPB_RICBR (Pyruvate dehydrogenase E1 component subunit beta OS=Rickettsia bellii (strain RML369-C) GN=pdhB PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 2.969e-37
Identity = 75/135 (55.56%), Postives = 104/135 (77.04%), Query Frame = -3

Query:   59 VPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTY 463
            +P GKAK+ ++G  VT++T S  V  A+ AA+ L+ + I+ EVI+LRTI+PLD+DTII SVKKTGR+V  EEG+   G+GA IAA++M+  FDYLDAPVE V+  DVP+PYA ++E LA+P  ++VINA+K+V Y
Sbjct:  189 IPFGKAKVLKEGDSVTIVTFSIQVKLALDAANILQSDNINCEVIDLRTIKPLDIDTIIESVKKTGRLVVIEEGWFFAGIGATIAAIVMKEAFDYLDAPVEIVSGKDVPLPYAVNLEKLALPSEYDVINAVKKVCY 323

[Close]

BLAST of GR409708 vs. TrEMBL
Match: A8TL70_9PROT (Pyruvate dehydrogenase E1 component, beta subunit OS=alpha proteobacterium BAL199 GN=BAL199_25714 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.517e-44
Identity = 88/140 (62.86%), Postives = 113/140 (80.71%), Query Frame = -3

Query:   53 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYRS 472
            D+++PIGKAKI R G DVT+   S MVG+A++AA++L  EGI  EVI+LRTIRPLD++TI+ SVKKT R+VT EEG+   G+G+EI+AL+MEH FDYLDAPV RVA ADVPMPYA ++E LA+PQ  N++ A+K V YRS
Sbjct:  335 DFVLPIGKAKIVRAGKDVTITAFSIMVGKALEAAEKLAEEGIEAEVIDLRTIRPLDIETIVTSVKKTNRLVTTEEGWAFSGIGSEISALMMEHAFDYLDAPVVRVAGADVPMPYAANLEKLALPQVDNIVQAVKAVCYRS 474

[Close]

BLAST of GR409708 vs. TrEMBL
Match: B6IQ33_RHOCS (Pyruvate dehydrogenase E1 component, beta subunit OS=Rhodospirillum centenum (strain ATCC 51521 / SW) GN=pdhB PE=4 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 2.131e-43
Identity = 85/139 (61.15%), Postives = 114/139 (82.01%), Query Frame = -3

Query:   56 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYR 472
            D++VPIG+AK+ RQG+DVTV  +S MV  A+ AA++L  EGISVEVI+LRTIRPLD++T++ASVKKT R+V+ EEG+   G+G+E+AAL+MEH FD+LDAPV RV   DVP+PYA ++E LA+PQ  +V+ A+K VTYR
Sbjct:  325 DFIVPIGRAKVVRQGTDVTVTAYSLMVAHALAAAERLAEEGISVEVIDLRTIRPLDVETVVASVKKTNRLVSVEEGWAFAGIGSELAALMMEHAFDHLDAPVVRVHAKDVPLPYAANLEKLALPQPDDVVQAVKAVTYR 463

[Close]

BLAST of GR409708 vs. TrEMBL
Match: A5EK04_BRASB (Pyruvate dehydrogenase E1 component, beta subunit OS=Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) GN=pdhB PE=4 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 2.783e-43
Identity = 89/139 (64.03%), Postives = 108/139 (77.70%), Query Frame = -3

Query:   56 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYR 472
            D+++PIGKA+I R G DVT+I+ S  +  A+KAAD+L  EGI  EVI+LRT+RP+D DTIIASVKKTGR VT EEG+ Q GVGAEIAA +MEH FDYLDAPV RV+  DVPMPYA ++E LA+P A  V+ A K V YR
Sbjct:  321 DYVIPIGKARIARSGKDVTIISWSNGMTYALKAADELAKEGIEAEVIDLRTLRPMDTDTIIASVKKTGRAVTVEEGWAQSGVGAEIAARIMEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSAAEVVQAAKSVCYR 459

[Close]

BLAST of GR409708 vs. TrEMBL
Match: A4YVB2_BRASO (Pyruvate dehydrogenase E1 component, beta subunit OS=Bradyrhizobium sp. (strain ORS278) GN=pdhB PE=4 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 2.783e-43
Identity = 89/139 (64.03%), Postives = 108/139 (77.70%), Query Frame = -3

Query:   56 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYR 472
            D+++PIGKA+I R G DVT+I+ S  +  A+KAAD+L  EGI  EVI+LRT+RP+D DTIIASVKKTGR VT EEG+ Q GVGAEIAA +MEH FDYLDAPV RV+  DVPMPYA ++E LA+P A  V+ A K V YR
Sbjct:  327 DYIIPIGKARIARTGKDVTIISWSNGMTYALKAADELAKEGIEAEVIDLRTLRPMDTDTIIASVKKTGRAVTVEEGWAQSGVGAEIAARIMEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSAAEVVQAAKSVCYR 465

[Close]

BLAST of GR409708 vs. TrEMBL
Match: B9GZC2_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_414358 PE=4 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 4.748e-43
Identity = 90/137 (65.69%), Postives = 108/137 (78.83%), Query Frame = -3

Query:   53 VPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYRS 463
            VPIGKAKIER+G DVT+   S+MVG A+KAA+ L  EGI+ EVINLR+IRPLD DTI ASV+KT R+VT EEGF Q GVGAEI A ++E  F YLDAPVER+A ADVPMPYA ++E LA+PQ  +++ A KR  YRS
Sbjct:  208 VPIGKAKIEREGKDVTITAFSKMVGYALKAAEILAKEGINAEVINLRSIRPLDRDTINASVRKTNRLVTVEEGFPQHGVGAEICASVVEESFGYLDAPVERIAGADVPMPYAANLERLAVPQVEDIVRAAKRACYRS 344

[Close]

BLAST of GR409708 vs. TrEMBL
Match: A9P841_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 4.748e-43
Identity = 90/137 (65.69%), Postives = 108/137 (78.83%), Query Frame = -3

Query:   53 VPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYRS 463
            VPIGKAKIER+G DVT+   S+MVG A+KAA+ L  EGI+ EVINLR+IRPLD DTI ASV+KT R+VT EEGF Q GVGAEI A ++E  F YLDAPVER+A ADVPMPYA ++E LA+PQ  +++ A KR  YRS
Sbjct:  230 VPIGKAKIEREGKDVTITAFSKMVGYALKAAEILAKEGINAEVINLRSIRPLDRDTINASVRKTNRLVTVEEGFPQHGVGAEICASVVEESFGYLDAPVERIAGADVPMPYAANLERLAVPQVEDIVRAAKRACYRS 366

[Close]

BLAST of GR409708 vs. TrEMBL
Match: D7MQB7_ARALY (Pyruvate dehydrogenase E1 component beta subunit, mitochondrial OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_331474 PE=4 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 8.098e-43
Identity = 90/138 (65.22%), Postives = 110/138 (79.71%), Query Frame = -3

Query:   50 VPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYRSK 463
            +PIGKAKIER+G DVT++T S+MVG A+KAA++L  EGIS EVINLR+IRPLD  TI ASV+KT R+VT EEGF Q GV AEI A ++E  F YLDAPVER+A ADVPMPYA ++E LA+PQ  +++ A KR  YRSK
Sbjct:  229 LPIGKAKIEREGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVCAEICASVVEESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIVRASKRACYRSK 366

[Close]

BLAST of GR409708 vs. TrEMBL
Match: Q07ND2_RHOP5 (Transketolase, central region OS=Rhodopseudomonas palustris (strain BisA53) GN=RPE_2614 PE=4 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 2.356e-42
Identity = 89/139 (64.03%), Postives = 110/139 (79.14%), Query Frame = -3

Query:   56 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYR 472
            D++VPIGKA++ER+G+ VT+I+ S  +  A+KAAD L  EGI  EVI+LRT+RPLD DTIIASVKKTGR VT EEG+ Q GVGAEIAA +MEH FDYLDAPV+RV+  DVPMPYA ++E LA+P    V++A K V YR
Sbjct:  326 DFVVPIGKARVEREGAHVTLISWSHGMTYALKAADALAKEGIDAEVIDLRTLRPLDTDTIIASVKKTGRAVTIEEGWQQNGVGAEIAARIMEHAFDYLDAPVKRVSGKDVPMPYAANLEKLALPSVAEVVDAAKAVCYR 464

[Close]

BLAST of GR409708 vs. TrEMBL
Match: C6T827_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 3.077e-42
Identity = 87/137 (63.50%), Postives = 107/137 (78.10%), Query Frame = -3

Query:   53 VPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYRS 463
            +PIGKAKIER+G DVT+  +S+MVG A+KAA+ L  EGIS EVINLR+IRPLD  TI ASV+KT R+VT EEGF Q GVGAEI   ++E  F YLDAPVER+A ADVPMPYA ++E +A+PQ  +++ A KR  YRS
Sbjct:  217 LPIGKAKIEREGKDVTITAYSKMVGYALKAAETLAKEGISAEVINLRSIRPLDRSTINASVRKTNRLVTVEEGFPQHGVGAEICTSVIEESFGYLDAPVERIAGADVPMPYAANLERMAVPQVEDIVRAAKRACYRS 353

[Close]

BLAST of GR409708 vs. TrEMBL
Match: Q214Z5_RHOPB (Transketolase, central region OS=Rhodopseudomonas palustris (strain BisB18) GN=RPC_2490 PE=4 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 4.019e-42
Identity = 89/139 (64.03%), Postives = 107/139 (76.98%), Query Frame = -3

Query:   56 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYR 472
            D++VPIGKAK+ R GS VT+I  S  +  A+KAAD+L  EGI  EVI+LRT+RPLD +TIIASVKKTGR VT EEG+ Q GVGAEIAA +MEH FDYLDAPV+RV+  DVPMPYA ++E LA+P    V+ A K V YR
Sbjct:  327 DYVVPIGKAKVARAGSHVTLIAWSNGMSYALKAADELAKEGIEAEVIDLRTLRPLDTETIIASVKKTGRAVTVEEGWQQNGVGAEIAARIMEHAFDYLDAPVKRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR 465

[Close]

BLAST of GR409708 vs. TAIR peptide
Match: AT5G50850.1 (| Symbols: MAB1 | Transketolase family protein | chr5:20689671-20692976 FORWARD LENGTH=363)

HSP 1 Score: 176.407 bits (446), Expect = 6.384e-45
Identity = 90/138 (65.22%), Postives = 110/138 (79.71%), Query Frame = -3

Query:   50 VPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRVTYRSK 463
            +PIGKAKIER+G DVT++T S+MVG A+KAA++L  EGIS EVINLR+IRPLD  TI ASV+KT R+VT EEGF Q GV AEI A ++E  F YLDAPVER+A ADVPMPYA ++E LA+PQ  +++ A KR  YRSK
Sbjct:  226 LPIGKAKIEREGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVCAEICASVVEESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIVRASKRACYRSK 363

[Close]

BLAST of GR409708 vs. TAIR peptide
Match: AT1G30120.1 (| Symbols: PDH-E1 BETA | pyruvate dehydrogenase E1 beta | chr1:10584350-10586477 REVERSE LENGTH=406)

HSP 1 Score: 102.834 bits (255), Expect = 8.970e-23
Identity = 52/136 (38.24%), Postives = 87/136 (63.97%), Query Frame = -3

Query:   65 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRV 472
            D++  + +A++ R G  +T++T+SRM    ++AA  L  +G   EVI++R+++P DL TI  SVKKT RV+  EE     G+GA + A + E+  DYLDAPV  ++  DVP PYA ++E+  + Q   ++ A++++
Sbjct:  269 DYVCNLEEAEMVRPGEHITILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFHDYLDAPVMCLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQL 404

[Close]

BLAST of GR409708 vs. TAIR peptide
Match: AT2G34590.1 (| Symbols: | Transketolase family protein | chr2:14568956-14570844 REVERSE LENGTH=406)

HSP 1 Score: 100.908 bits (250), Expect = 3.409e-22
Identity = 51/136 (37.50%), Postives = 87/136 (63.97%), Query Frame = -3

Query:   65 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIKRV 472
            +++  + +A++ R G  +T++T+SRM    ++AA  L  +G   EVI++R+++P DL TI  SVKKT RV+  EE     G+GA + A + E+  DYLDAPV  ++  DVP PYA ++E+  + Q   ++ A++++
Sbjct:  269 EYICNLEEAEMVRPGEHITILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLYTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFHDYLDAPVMCLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQL 404

[Close]

BLAST of GR409708 vs. TAIR peptide
Match: AT1G55510.1 (| Symbols: BCDH BETA1 | branched-chain alpha-keto acid decarboxylase E1 beta subunit | chr1:20723482-20725505 FORWARD LENGTH=352)

HSP 1 Score: 100.908 bits (250), Expect = 3.409e-22
Identity = 52/134 (38.81%), Postives = 85/134 (63.43%), Query Frame = -3

Query:   71 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIK 472
            D+++P+ +A++ R+G+D+T++     +    +A    + EGIS E+I+L+T+ P D +T+ ASVKKTGR++ + E  V  G GAEI+A ++E CF  L+APV RV   D P P     E   +P  + +++AIK
Sbjct:  216 DYMIPLSEAEVIREGNDITLVGWGAQLTVMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISATILERCFLKLEAPVSRVCGLDTPFPLV--FEPFYMPTKNKILDAIK 347

[Close]

BLAST of GR409708 vs. TAIR peptide
Match: AT3G13450.1 (| Symbols: DIN4 | Transketolase family protein | chr3:4382340-4384295 REVERSE LENGTH=358)

HSP 1 Score: 97.0561 bits (240), Expect = 4.922e-21
Identity = 50/134 (37.31%), Postives = 83/134 (61.94%), Query Frame = -3

Query:   71 DWLVPIGKAKIERQGSDVTVITHSRMVGEAIKAADQLKGEGISVEVINLRTIRPLDLDTIIASVKKTGRVVTAEEGFVQCGVGAEIAALLMEHCFDYLDAPVERVAVADVPMPYAKSIEDLAIPQAHNVINAIK 472
            D+++P+ +A++ R+GSD+T++     +    +A    + EGIS E+I+L+T+ P D + +  SV+KTGR++ + E  V  G GAEIAA ++E CF  L+APV RV   D P P     E   +P  + +++AI+
Sbjct:  222 DYMIPLSEAEVMREGSDITLVGWGAQLTIMEQACLDAENEGISCELIDLKTLIPWDKEIVETSVRKTGRLLISHEAPVTGGFGAEIAATIVERCFLRLEAPVSRVCGLDTPFPLV--FEPFYMPTKNKILDAIR 353

The following BLAST results are available for this feature:

BLAST of GR409708 vs. SwissProt
BLAST of GR409708 vs. TrEMBL
BLAST of GR409708 vs. TAIR peptide

BLAST of GR409708 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ODPB_DICDI	2.679e-46	60.99	Pyruvate dehydrogenase E1 component subunit beta, ...	[more]
ODPB_ARATH	5.585e-44	65.22	Pyruvate dehydrogenase E1 component subunit beta, ...	[more]
ODPB_PEA	3.746e-40	61.15	Pyruvate dehydrogenase E1 component subunit beta, ...	[more]
ODPB_PONAB	2.685e-38	59.56	Pyruvate dehydrogenase E1 component subunit beta, ...	[more]
ODPB_HUMAN	2.685e-38	59.56	Pyruvate dehydrogenase E1 component subunit beta, ...	[more]
ODPB_BOVIN	4.580e-38	59.56	Pyruvate dehydrogenase E1 component subunit beta, ...	[more]
ODPB_RAT	5.981e-38	60.29	Pyruvate dehydrogenase E1 component subunit beta, ...	[more]
ODPB_RHIME	1.020e-37	53.96	Pyruvate dehydrogenase E1 component subunit beta O...	[more]
ODPB_MOUSE	1.020e-37	58.82	Pyruvate dehydrogenase E1 component subunit beta, ...	[more]
ODPB_RICBR	2.969e-37	55.56	Pyruvate dehydrogenase E1 component subunit beta O...	[more]

BLAST of GR409708 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A8TL70_9PROT	2.517e-44	62.86	Pyruvate dehydrogenase E1 component, beta subunit ...	[more]
B6IQ33_RHOCS	2.131e-43	61.15	Pyruvate dehydrogenase E1 component, beta subunit ...	[more]
A5EK04_BRASB	2.783e-43	64.03	Pyruvate dehydrogenase E1 component, beta subunit ...	[more]
A4YVB2_BRASO	2.783e-43	64.03	Pyruvate dehydrogenase E1 component, beta subunit ...	[more]
B9GZC2_POPTR	4.748e-43	65.69	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
A9P841_POPTR	4.748e-43	65.69	Putative uncharacterized protein OS=Populus tricho...	[more]
D7MQB7_ARALY	8.098e-43	65.22	Pyruvate dehydrogenase E1 component beta subunit, ...	[more]
Q07ND2_RHOP5	2.356e-42	64.03	Transketolase, central region OS=Rhodopseudomonas ...	[more]
C6T827_SOYBN	3.077e-42	63.50	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q214Z5_RHOPB	4.019e-42	64.03	Transketolase, central region OS=Rhodopseudomonas ...	[more]

BLAST of GR409708 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G50850.1	6.384e-45	65.22	\| Symbols: MAB1 \| Transketolase family protein \| c...	[more]
AT1G30120.1	8.970e-23	38.24	\| Symbols: PDH-E1 BETA \| pyruvate dehydrogenase E1...	[more]
AT2G34590.1	3.409e-22	37.50	\| Symbols: \| Transketolase family protein \| chr2:...	[more]
AT1G55510.1	3.409e-22	38.81	\| Symbols: BCDH BETA1 \| branched-chain alpha-keto ...	[more]
AT3G13450.1	4.922e-21	37.31	\| Symbols: DIN4 \| Transketolase family protein \| c...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 52..68 score
None	No IPR available	SEG	seg	seg	coord: 142..153 score
None	No IPR available	PANTHER	PTHR11624	DEHYDROGENASE RELATED	coord: 1..141 score: 1
None	No IPR available	PANTHER	PTHR11624:SF11	PYRUVATE DEHYDROGENASE E1 COMPONENT, BETA SUBUNIT	coord: 1..141 score: 1
IPR005476	Transketolase, C-terminal	PFAM	PF02780	Transketolase_C	coord: 7..126 score: 6.2
IPR009014	Transketolase, C-terminal/Pyruvate-ferredoxin oxidoreductase, domain II	SUPERFAMILY	52922	TK C-terminal domain-like	coord: 3..136 score: 2.7
IPR015941	Transketolase-like, C-terminal	GENE3D	3.40.50.920	no description	coord: 6..136 score: 2.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR409708 ID=GR409708; Name=GR409708; organism=Cicer arietinum; type=EST; length=474bp
GATGAGTAGTTTATTACATATCTTTCAATCAACATACAAAACTGTCTTAT
TTGCTGCGGTAAGTGACGCGCTTGATCGCATTGATGACGTTGTGTGCTTG
AGGAATGGCCAGATCTTCAATCGACTTGGCGTAGGGCATGGGCACATCAG
CCACGGCCACACGCTCCACGGGGGCATCGAGGTAATCGAAGCAGTGCTCC
ATCAACAGCGCGGCAATCTCCGCACCCACACCGCACTGCACGAAGCCCTC
TTCGGCAGTCACCACGCGACCAGTCTTCTTCACCGAGGCGATGATCGTGT
CCAGATCCAACGGTCTGATCGTGCGCAAGTTGATCACCTCCACGCTGATA
CCCTCGCCCTTGAGCTGATCAGCGGCCTTGATGGCTTCACCGACCATTCT
GCTGTGCGTGATCACCGTCACATCGCTGCCCTGGCGTTCAATCTTCGCCT
TGCCAATCGGTACCAACCAGTCCG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR005476	Transketolase, C-terminal
IPR009014	Transketo_C/PFOR_II
IPR015941	Transketolase-like, C-terminal

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