HO063733

EST Overview
NameHO063733
Unique NameHO063733
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length285
Libraries
Library NameType
LIBEST_026410 Chickpea drought stressed cDNA SSH library AR1cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of HO063733 vs. TrEMBL
Match: Q43791_MEDSA (Peroxidase1C OS=Medicago sativa GN=prx1C PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.536e-9
Identity = 34/68 (50.00%), Postives = 36/68 (52.94%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNELIYGIA*TM-PHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  TG P PTLN        T+ P+         FDP  P   DKNYY  LQ K GLLQSD ELFS
Sbjct:  210 YNFSSTGSPDPTLNTTYLQQLRTICPNGGPGTNLTNFDPTTPDKFDKNYYSNLQVKKGLLQSDQELFS 277

HSP 2 Score: 30.4166 bits (67), Expect = 1.536e-9
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYST 265
            F  SGADTISIVNK+ST
Sbjct:  276 FSTSGADTISIVNKFST 292
[Close]
BLAST of HO063733 vs. TrEMBL
Match: Q40365_MEDSA (Peroxidase OS=Medicago sativa GN=pxdA PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 5.656e-9
Identity = 34/68 (50.00%), Postives = 36/68 (52.94%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNELIYGIA*TM-PHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  TG P PTLN        T+ P+         FDP  P   DKNYY  LQ K GLLQSD ELFS
Sbjct:  202 YNFSSTGSPDPTLNTTYLQQLRTICPNGGPGTNLTNFDPTTPDKFDKNYYSNLQVKKGLLQSDQELFS 269

HSP 2 Score: 28.4906 bits (62), Expect = 5.656e-9
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYST 265
            F  SGADTISIV+K+ST
Sbjct:  268 FSTSGADTISIVDKFST 284
[Close]
BLAST of HO063733 vs. TrEMBL
Match: A4UN78_MEDTR (Peroxidase OS=Medicago truncatula GN=PRX3 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 7.333e-9
Identity = 34/68 (50.00%), Postives = 36/68 (52.94%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNELIYGIA*TM-PHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  TG P PTLN        T+ P+         FDP  P   DKNYY  LQ K GLLQSD ELFS
Sbjct:  212 YNFNGTGSPDPTLNTTYLQQLRTICPNGGPGTNLTNFDPTTPDKFDKNYYSNLQVKKGLLQSDQELFS 279

HSP 2 Score: 28.1054 bits (61), Expect = 7.333e-9
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYST 265
            F  SG+DTISIVNK++T
Sbjct:  278 FSTSGSDTISIVNKFAT 294
[Close]
BLAST of HO063733 vs. TrEMBL
Match: Q43790_MEDSA (Peroxidase1B OS=Medicago sativa GN=prx1B PE=2 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 9.515e-9
Identity = 34/68 (50.00%), Postives = 36/68 (52.94%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNELIYGIA*TM-PHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  TG P PTLN        T+ P+         FDP  P   DKNYY  LQ K GLLQSD ELFS
Sbjct:  211 YNFSGTGSPDPTLNTTYLQQLRTICPNGGPGTNLTNFDPTTPDKFDKNYYSNLQVKKGLLQSDQELFS 278

HSP 2 Score: 28.1054 bits (61), Expect = 9.515e-9
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYST 265
            F  SG+DTISIVNK++T
Sbjct:  277 FSTSGSDTISIVNKFAT 293
[Close]
BLAST of HO063733 vs. TrEMBL
Match: Q18PQ7_PEA (Peroxidase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 9.519e-9
Identity = 33/68 (48.53%), Postives = 37/68 (54.41%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNEL-IYGIA*TMPHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  TG P P+LN   +  +  T P   +      FDP  P   DKNYY  LQ K GLLQSD ELFS
Sbjct:  211 YNFSNTGKPDPSLNTTYLQELRKTCPKGGSGTNLANFDPTTPDRFDKNYYSNLQVKKGLLQSDQELFS 278

HSP 2 Score: 27.335 bits (59), Expect = 9.519e-9
Identity = 12/16 (75.00%), Postives = 14/16 (87.50%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYS 262
            F  SGADTI+IVNK+S
Sbjct:  277 FSTSGADTITIVNKFS 292
[Close]
BLAST of HO063733 vs. TrEMBL
Match: Q76KW0_PEA (Peroxidase (Fragment) OS=Pisum sativum GN=PRX PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 9.595e-9
Identity = 33/68 (48.53%), Postives = 37/68 (54.41%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNEL-IYGIA*TMPHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  TG P P+LN   +  +  T P   +      FDP  P   DKNYY  LQ K GLLQSD ELFS
Sbjct:  132 YNFSNTGKPDPSLNTTYLQELRKTCPKGGSGTNLANFDPTTPDRFDKNYYSNLQVKKGLLQSDQELFS 199

HSP 2 Score: 27.335 bits (59), Expect = 9.595e-9
Identity = 12/16 (75.00%), Postives = 14/16 (87.50%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYS 262
            F  SGADTI+IVNK+S
Sbjct:  198 FSTSGADTITIVNKFS 213
[Close]
BLAST of HO063733 vs. TrEMBL
Match: C6TI50_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 3.496e-8
Identity = 33/68 (48.53%), Postives = 37/68 (54.41%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNEL-IYGIA*TMPHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  TG P PTL+   +  +    P     +    FDP  P T DKNYY  LQ K GLLQSD ELFS
Sbjct:  208 YNFSGTGRPDPTLDTTYLKQLRQICPQGGPPNNLVNFDPTTPDTLDKNYYSNLQVKKGLLQSDQELFS 275

HSP 2 Score: 26.9498 bits (58), Expect = 3.496e-8
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYST 265
            F   GADTISIVNK+S+
Sbjct:  274 FSTPGADTISIVNKFSS 290
[Close]
BLAST of HO063733 vs. TrEMBL
Match: Q18PQ8_PEA (Peroxidase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 4.534e-8
Identity = 31/68 (45.59%), Postives = 37/68 (54.41%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNEL-IYGIA*TMPHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  TG+P PT+N   +  +    P+  +      FDP      DKNYY  LQ K GLLQSD ELFS
Sbjct:  209 YNFSNTGNPDPTVNTTYLQELRNICPNGGSGTNLANFDPTTADKFDKNYYSNLQVKKGLLQSDQELFS 276

HSP 2 Score: 28.4906 bits (62), Expect = 4.534e-8
Identity = 13/16 (81.25%), Postives = 14/16 (87.50%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYS 262
            F  SGADTISIVNK+S
Sbjct:  275 FSTSGADTISIVNKFS 290
[Close]
BLAST of HO063733 vs. TrEMBL
Match: C6TJD7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 9.895e-8
Identity = 32/68 (47.06%), Postives = 37/68 (54.41%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNEL-IYGIA*TMPHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  TG+P PTLN   +  ++   P+         FDP  P T D NYY  LQ   GLLQSD ELFS
Sbjct:  206 YNFSSTGNPDPTLNTTYLQTLSAICPNGGPGTNLTNFDPTTPDTVDSNYYSNLQVNKGLLQSDQELFS 273

HSP 2 Score: 24.6386 bits (52), Expect = 9.895e-8
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYST 265
            F  +GADTI+IVN +S+
Sbjct:  272 FSTTGADTIAIVNSFSS 288
[Close]
BLAST of HO063733 vs. TrEMBL
Match: Q8GZS1_LUPAL (Extensin peroxidase OS=Lupinus albus PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.663e-7
Identity = 32/68 (47.06%), Postives = 36/68 (52.94%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNELIYGIA*TM-PHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFS 221
            YNF  +G P P+LN        T+ P+         FDP  P T DKNYY  LQ   GLLQSD ELFS
Sbjct:  212 YNFSNSGSPDPSLNTTYLQTLRTICPNGGAGTNLTNFDPTTPDTFDKNYYSNLQVHKGLLQSDQELFS 279

HSP 2 Score: 25.409 bits (54), Expect = 1.663e-7
Identity = 11/17 (64.71%), Postives = 13/17 (76.47%), Query Frame = 2 
Query:  215 FFPSGADTISIVNKYST 265
            F  +GADTIS VN +ST
Sbjct:  278 FSTTGADTISTVNSFST 294
[Close]
BLAST of HO063733 vs. TAIR peptide
Match: AT5G06730.1 (| Symbols: | Peroxidase superfamily protein | chr5:2080207-2081621 REVERSE LENGTH=358)

HSP 1 Score: 49.2914 bits (116), Expect = 5.698e-7
Identity = 28/69 (40.58%), Postives = 37/69 (53.62%), Query Frame = 3 
Query:   21 YNFRYTGDPVPTLNE-LIYGIA*TMPHEWTRD*PPYFDPAAPVTSDKNYYPPLQAKTGLLQSDHELFSH 224
            +NF  TG+P PTLN  L+  +    P   +       D + P   D NY+  LQ+  GLLQSD ELFS+
Sbjct:  215 FNFNGTGNPDPTLNSTLLSSLQQLCPQNGSNTGITNLDLSTPDAFDNNYFTNLQSNNGLLQSDQELFSN 283
The following BLAST results are available for this feature:
BLAST of HO063733 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q43791_MEDSA1.536e-950.00Peroxidase1C OS=Medicago sativa GN=prx1C PE=2 SV=1[more]
Q40365_MEDSA5.656e-950.00Peroxidase OS=Medicago sativa GN=pxdA PE=2 SV=1[more]
A4UN78_MEDTR7.333e-950.00Peroxidase OS=Medicago truncatula GN=PRX3 PE=2 SV=... [more]
Q43790_MEDSA9.515e-950.00Peroxidase1B OS=Medicago sativa GN=prx1B PE=2 SV=1[more]
Q18PQ7_PEA9.519e-948.53Peroxidase OS=Pisum sativum PE=2 SV=1[more]
Q76KW0_PEA9.595e-948.53Peroxidase (Fragment) OS=Pisum sativum GN=PRX PE=2... [more]
C6TI50_SOYBN3.496e-848.53Putative uncharacterized protein OS=Glycine max PE... [more]
Q18PQ8_PEA4.534e-845.59Peroxidase OS=Pisum sativum PE=2 SV=1[more]
C6TJD7_SOYBN9.895e-847.06Putative uncharacterized protein OS=Glycine max PE... [more]
Q8GZS1_LUPAL1.663e-747.06Extensin peroxidase OS=Lupinus albus PE=2 SV=1[more]
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BLAST of HO063733 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT5G06730.15.698e-740.58| Symbols: | Peroxidase superfamily protein | chr... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 20..38
score
IPR000823Plant peroxidasePRINTSPR00461PLPEROXIDASEcoord: 3..18
score: 8.6e-05coord: 19..36
score: 8.6

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO063733 ID=HO063733; Name=HO063733; organism=Cicer arietinum; type=EST; length=285bp
TAGCGTGGTCGCGGCCGAGGTACAATTTTAGATATACTGGTGATCCTGTG
CCTACTCTAAACGAACTTATTTACGGAATTGCGTAAACTATGCCCCATGA
GTGGACCAGGGACTAACCTCCTTATTTTGATCCAGCCGCTCCTGTTACAT
CCGACAAAAACTACTACCCCCCTCTACAGGCTAAAACGGGTTTACTTCAG
AGTGATCATGAGCTTTTTTCCCATCTGGTGCAGATACTATTAGTATTGTC
AACAAGTATAGTACCTGCCCGGGCGGCCGCTCCAG
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004601peroxidase activity
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0006979response to oxidative stress
GO:0055114obsolete oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR000823Peroxidase_pln