HO664161

EST Overview
NameHO664161
Unique NameHO664161
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length229
Libraries
Library NameType
LIBEST_026619 TDZ treated Chickpea SSH librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
Homology
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A_VICFA (Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.134e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A_TOBAC (Elongation factor 1-alpha OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.134e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A_SOYBN (Elongation factor 1-alpha OS=Glycine max GN=TEFS1 PE=3 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 5.134e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A_SOLLC (Elongation factor 1-alpha OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.134e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A_ORYSJ (Elongation factor 1-alpha OS=Oryza sativa subsp. japonica GN=REFA1 PE=2 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 5.134e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A_MANES (Elongation factor 1-alpha OS=Manihot esculenta GN=EF1 PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.134e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana GN=A1 PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.134e-15
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A_WHEAT (Elongation factor 1-alpha OS=Triticum aestivum GN=TEF1 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.705e-15
Identity = 34/35 (97.14%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDK+PFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKVPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A_PEA (Elongation factor 1-alpha OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.494e-14
Identity = 34/35 (97.14%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLK+VGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKEVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. SwissProt
Match: EF1A2_HORVU (Elongation factor 1-alpha OS=Hordeum vulgare GN=BLT63 PE=1 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.951e-14
Identity = 33/35 (94.29%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDK+PFVPISGFEGDNMIERS+NLDWY
Sbjct:  177 YLKKVGYNPDKVPFVPISGFEGDNMIERSSNLDWY 211
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q9ZWH9_NICPA (Elongation factor 1-alpha OS=Nicotiana paniculata GN=NpeEF1A PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q9ZRP9_MALDO (Elongation factor 1-alpha OS=Malus domestica GN=eF-1alpha PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q9SPA1_LILLO (Elongation factor 1-alpha OS=Lilium longiflorum PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q9M516_CAPAN (Elongation factor 1-alpha OS=Capsicum annuum PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  176 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 210
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q9LN13_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211

HSP 2 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  695 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 729
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q9C5L4_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana GN=At5g60390 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q9ASU9_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q94AD0_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q8W4H7_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana GN=At1g07920/T6D22.2 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TrEMBL
Match: Q8W0W2_ELAOL (Elongation factor 1-alpha OS=Elaeis oleifera PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.034e-13
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TAIR peptide
Match: AT5G60390.3 (| Symbols: | GTP binding Elongation factor Tu family protein | chr5:24289226-24290675 FORWARD LENGTH=449)

HSP 1 Score: 79.7221 bits (195), Expect = 3.989e-16
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TAIR peptide
Match: AT5G60390.2 (| Symbols: | GTP binding Elongation factor Tu family protein | chr5:24289226-24290577 FORWARD LENGTH=400)

HSP 1 Score: 79.7221 bits (195), Expect = 3.989e-16
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TAIR peptide
Match: AT5G60390.1 (| Symbols: | GTP binding Elongation factor Tu family protein | chr5:24289226-24290675 FORWARD LENGTH=449)

HSP 1 Score: 79.7221 bits (195), Expect = 3.989e-16
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TAIR peptide
Match: AT1G07930.2 (| Symbols: | GTP binding Elongation factor Tu family protein | chr1:2459014-2460458 FORWARD LENGTH=372)

HSP 1 Score: 79.7221 bits (195), Expect = 3.989e-16
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TAIR peptide
Match: AT1G07940.2 (| Symbols: | GTP binding Elongation factor Tu family protein | chr1:2463350-2464792 REVERSE LENGTH=449)

HSP 1 Score: 79.7221 bits (195), Expect = 3.989e-16
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TAIR peptide
Match: AT1G07940.1 (| Symbols: | GTP binding Elongation factor Tu family protein | chr1:2463350-2464792 REVERSE LENGTH=449)

HSP 1 Score: 79.7221 bits (195), Expect = 3.989e-16
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TAIR peptide
Match: AT1G07930.1 (| Symbols: | GTP binding Elongation factor Tu family protein | chr1:2459014-2460458 FORWARD LENGTH=449)

HSP 1 Score: 79.7221 bits (195), Expect = 3.989e-16
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
[Close]
BLAST of HO664161 vs. TAIR peptide
Match: AT1G07920.1 (| Symbols: | GTP binding Elongation factor Tu family protein | chr1:2455559-2457001 FORWARD LENGTH=449)

HSP 1 Score: 79.7221 bits (195), Expect = 3.989e-16
Identity = 35/35 (100.00%), Postives = 35/35 (100.00%), Query Frame = 1 
Query:    1 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 105
            YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY
Sbjct:  177 YLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
The following BLAST results are available for this feature:
BLAST of HO664161 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
EF1A_VICFA5.134e-15100.00Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1[more]
EF1A_TOBAC5.134e-15100.00Elongation factor 1-alpha OS=Nicotiana tabacum PE=... [more]
EF1A_SOYBN5.134e-15100.00Elongation factor 1-alpha OS=Glycine max GN=TEFS1 ... [more]
EF1A_SOLLC5.134e-15100.00Elongation factor 1-alpha OS=Solanum lycopersicum ... [more]
EF1A_ORYSJ5.134e-15100.00Elongation factor 1-alpha OS=Oryza sativa subsp. j... [more]
EF1A_MANES5.134e-15100.00Elongation factor 1-alpha OS=Manihot esculenta GN=... [more]
EF1A_ARATH5.134e-15100.00Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
EF1A_WHEAT6.705e-1597.14Elongation factor 1-alpha OS=Triticum aestivum GN=... [more]
EF1A_PEA1.494e-1497.14Elongation factor 1-alpha OS=Pisum sativum PE=2 SV... [more]
EF1A2_HORVU1.951e-1494.29Elongation factor 1-alpha OS=Hordeum vulgare GN=BL... [more]
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BLAST of HO664161 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9ZWH9_NICPA1.034e-13100.00Elongation factor 1-alpha OS=Nicotiana paniculata ... [more]
Q9ZRP9_MALDO1.034e-13100.00Elongation factor 1-alpha OS=Malus domestica GN=eF... [more]
Q9SPA1_LILLO1.034e-13100.00Elongation factor 1-alpha OS=Lilium longiflorum PE... [more]
Q9M516_CAPAN1.034e-13100.00Elongation factor 1-alpha OS=Capsicum annuum PE=2 ... [more]
Q9LN13_ARATH1.034e-13100.00Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
Q9C5L4_ARATH1.034e-13100.00Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
Q9ASU9_ARATH1.034e-13100.00Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
Q94AD0_ARATH1.034e-13100.00Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
Q8W4H7_ARATH1.034e-13100.00Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
Q8W0W2_ELAOL1.034e-13100.00Elongation factor 1-alpha OS=Elaeis oleifera PE=2 ... [more]
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BLAST of HO664161 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 8
Match NameE-valueIdentityDescription
AT5G60390.33.989e-16100.00| Symbols: | GTP binding Elongation factor Tu fam... [more]
AT5G60390.23.989e-16100.00| Symbols: | GTP binding Elongation factor Tu fam... [more]
AT5G60390.13.989e-16100.00| Symbols: | GTP binding Elongation factor Tu fam... [more]
AT1G07930.23.989e-16100.00| Symbols: | GTP binding Elongation factor Tu fam... [more]
AT1G07940.23.989e-16100.00| Symbols: | GTP binding Elongation factor Tu fam... [more]
AT1G07940.13.989e-16100.00| Symbols: | GTP binding Elongation factor Tu fam... [more]
AT1G07930.13.989e-16100.00| Symbols: | GTP binding Elongation factor Tu fam... [more]
AT1G07920.13.989e-16100.00| Symbols: | GTP binding Elongation factor Tu fam... [more]
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Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO664161 ID=HO664161; Name=HO664161; organism=Cicer arietinum; type=EST; length=229bp
TACTTGAAGAAGGTTGGTTACAACCCTGACAAAATCCCCTTCGTTCCAAT
CTCTGGTTTTGAGGGAGACAACATGATTGAGAGGTCCACCAACCTTGACT
GGTACCTCCTATAATGATATTGGGTTTAATTTACTATTGGAACAACCAGT
TGTGACCTATATATATATATGTAATATACAGACTTATCTGAGTTGTTCCT
TGCTTCATATGTACCTCGGCCGCGACCAC
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