ES560253

EST Overview


Name	ES560253
Unique Name	ES560253
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	244

Libraries


Library Name	Type
LIBEST_021248 Ascochyta rabiei induced library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A3_MEDTR (Probable histone H2A.3 OS=Medicago truncatula PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.891e-10
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   25 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 54

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A2_MEDTR (Probable histone H2A.2 OS=Medicago truncatula GN=MtrDRAFT_AC149210g4v1 PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.891e-10
Identity = 30/30 (100.00%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   24 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 53

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A3_WHEAT (Histone H2A.2.2 OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.090e-9
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRSI+AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   22 VTRSIKAGLQFPVGRIGRYLKKGRYAQRVG 51

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A_PETCR (Histone H2A OS=Petroselinum crispum PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.423e-9
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   23 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 52

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A5_WHEAT (Protein H2A.5 OS=Triticum aestivum GN=H2A-2 PE=2 SV=3)

HSP 1 Score: 61.6178 bits (148), Expect = 1.423e-9
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   13 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 42

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A1_WHEAT (Histone H2A.1 OS=Triticum aestivum GN=H2A-9 PE=1 SV=2)

HSP 1 Score: 61.6178 bits (148), Expect = 1.423e-9
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   13 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 42

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A2_PEA (Histone H2A.2 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.859e-9
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS+RAGLQFPVGRIGR+LKKGRYAQRVG
Sbjct:   24 VTRSVRAGLQFPVGRIGRFLKKGRYAQRVG 53

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A1_MEDTR (Probable histone H2A.1 OS=Medicago truncatula GN=MtrDRAFT_AC149210g1v1 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.859e-9
Identity = 29/30 (96.67%), Postives = 29/30 (96.67%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS RAGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   23 VTRSTRAGLQFPVGRIGRYLKKGRYAQRVG 52

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A2_WHEAT (Histone H2A.2.1 OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.427e-9
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VT+SI+AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   22 VTKSIKAGLQFPVGRIGRYLKKGRYAQRVG 51

[Close]

BLAST of ES560253 vs. SwissProt
Match: H2A1_SOLLC (Histone H2A.1 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.427e-9
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VT+SI+AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   22 VTKSIKAGLQFPVGRIGRYLKKGRYAQRVG 51

[Close]

BLAST of ES560253 vs. TrEMBL
Match: B9T4D5_RICCO (Histone H2A OS=Ricinus communis GN=RCOM_0390300 PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.333e-8
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS+RAGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   24 VTRSVRAGLQFPVGRIGRYLKKGRYAQRVG 53

[Close]

BLAST of ES560253 vs. TrEMBL
Match: B9T4D3_RICCO (Histone H2A OS=Ricinus communis GN=RCOM_0390180 PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.333e-8
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS+RAGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   24 VTRSVRAGLQFPVGRIGRYLKKGRYAQRVG 53

[Close]

BLAST of ES560253 vs. TrEMBL
Match: C5XBR4_SORBI (Histone H2A OS=Sorghum bicolor GN=Sb02g004970 PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.971e-8
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   30 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 59

[Close]

BLAST of ES560253 vs. TrEMBL
Match: B6U5W4_MAIZE (Histone H2A OS=Zea mays PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.971e-8
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   29 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 58

[Close]

BLAST of ES560253 vs. TrEMBL
Match: B6T832_MAIZE (Histone H2A OS=Zea mays PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.971e-8
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   29 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 58

[Close]

BLAST of ES560253 vs. TrEMBL
Match: B6T3E9_MAIZE (Histone H2A OS=Zea mays PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.971e-8
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   29 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 58

[Close]

BLAST of ES560253 vs. TrEMBL
Match: B6T0P6_MAIZE (Histone H2A OS=Zea mays PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.971e-8
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   29 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 58

[Close]

BLAST of ES560253 vs. TrEMBL
Match: B6SYW4_MAIZE (Histone H2A OS=Zea mays PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.971e-8
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   29 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 58

[Close]

BLAST of ES560253 vs. TrEMBL
Match: B6SYU0_MAIZE (Histone H2A OS=Zea mays PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.971e-8
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   29 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 58

[Close]

BLAST of ES560253 vs. TrEMBL
Match: B6SJU3_MAIZE (Histone H2A OS=Zea mays PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.971e-8
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            VTRS++AGLQFPVGRIGRYLKKGRYAQRVG
Sbjct:   29 VTRSVKAGLQFPVGRIGRYLKKGRYAQRVG 58

[Close]

BLAST of ES560253 vs. TAIR peptide
Match: AT5G02560.2 (| Symbols: HTA12 | histone H2A 12 | chr5:575437-576456 FORWARD LENGTH=177)

HSP 1 Score: 56.225 bits (134), Expect = 4.837e-9
Identity = 24/30 (80.00%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            V+RS+++GLQFPVGRIGRYLKKGRY++RVG
Sbjct:   25 VSRSVKSGLQFPVGRIGRYLKKGRYSKRVG 54

[Close]

BLAST of ES560253 vs. TAIR peptide
Match: AT5G02560.1 (| Symbols: HTA12 | histone H2A 12 | chr5:575437-576456 FORWARD LENGTH=153)

HSP 1 Score: 56.225 bits (134), Expect = 4.837e-9
Identity = 24/30 (80.00%), Postives = 30/30 (100.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            V+RS+++GLQFPVGRIGRYLKKGRY++RVG
Sbjct:   25 VSRSVKSGLQFPVGRIGRYLKKGRYSKRVG 54

[Close]

BLAST of ES560253 vs. TAIR peptide
Match: AT5G59870.1 (| Symbols: HTA6 | histone H2A 6 | chr5:24115605-24116144 REVERSE LENGTH=150)

HSP 1 Score: 52.373 bits (124), Expect = 6.985e-8
Identity = 23/30 (76.67%), Postives = 29/30 (96.67%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            V++S++AGLQFPVGRI R+LKKGRYAQR+G
Sbjct:   25 VSKSMKAGLQFPVGRITRFLKKGRYAQRLG 54

[Close]

BLAST of ES560253 vs. TAIR peptide
Match: AT5G27670.1 (| Symbols: HTA7 | histone H2A 7 | chr5:9792807-9793365 REVERSE LENGTH=150)

HSP 1 Score: 51.9878 bits (123), Expect = 9.123e-8
Identity = 23/30 (76.67%), Postives = 27/30 (90.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            V++S++AGLQFPVGRI RYLKKGRYA R G
Sbjct:   26 VSKSVKAGLQFPVGRIARYLKKGRYALRYG 55

[Close]

BLAST of ES560253 vs. TAIR peptide
Match: AT1G51060.1 (| Symbols: HTA10 | histone H2A 10 | chr1:18926948-18927443 FORWARD LENGTH=132)

HSP 1 Score: 51.9878 bits (123), Expect = 9.123e-8
Identity = 23/29 (79.31%), Postives = 27/29 (93.10%), Query Frame = 2

Query:  158 TRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            TRS +AGLQFPVGRI R+LKKG+YA+RVG
Sbjct:   18 TRSSKAGLQFPVGRIARFLKKGKYAERVG 46

[Close]

BLAST of ES560253 vs. TAIR peptide
Match: AT1G08880.1 (| Symbols: HTA5, H2AXA, G-H2AX, GAMMA-H2AX | Histone superfamily protein | chr1:2847144-2847676 REVERSE LENGTH=142)

HSP 1 Score: 50.0618 bits (118), Expect = 3.467e-7
Identity = 22/30 (73.33%), Postives = 27/30 (90.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            V+RS +AGLQFPVGRI R+LK G+YA+RVG
Sbjct:   23 VSRSSKAGLQFPVGRIARFLKSGKYAERVG 52

[Close]

BLAST of ES560253 vs. TAIR peptide
Match: AT1G54690.1 (| Symbols: HTA3, H2AXB, G-H2AX, GAMMA-H2AX | gamma histone variant H2AX | chr1:20414607-20415206 REVERSE LENGTH=142)

HSP 1 Score: 49.6766 bits (117), Expect = 4.527e-7
Identity = 22/30 (73.33%), Postives = 27/30 (90.00%), Query Frame = 2

Query:  155 VTRSIRAGLQFPVGRIGRYLKKGRYAQRVG 244
            V+RS +AGLQFPVGRI R+LK G+YA+RVG
Sbjct:   23 VSRSSKAGLQFPVGRIARFLKAGKYAERVG 52

The following BLAST results are available for this feature:

BLAST of ES560253 vs. SwissProt
BLAST of ES560253 vs. TrEMBL
BLAST of ES560253 vs. TAIR peptide

BLAST of ES560253 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
H2A3_MEDTR	4.891e-10	100.00	Probable histone H2A.3 OS=Medicago truncatula PE=3...	[more]
H2A2_MEDTR	4.891e-10	100.00	Probable histone H2A.2 OS=Medicago truncatula GN=M...	[more]
H2A3_WHEAT	1.090e-9	96.67	Histone H2A.2.2 OS=Triticum aestivum PE=1 SV=1	[more]
H2A_PETCR	1.423e-9	93.33	Histone H2A OS=Petroselinum crispum PE=2 SV=1	[more]
H2A5_WHEAT	1.423e-9	93.33	Protein H2A.5 OS=Triticum aestivum GN=H2A-2 PE=2 S...	[more]
H2A1_WHEAT	1.423e-9	93.33	Histone H2A.1 OS=Triticum aestivum GN=H2A-9 PE=1 S...	[more]
H2A2_PEA	1.859e-9	93.33	Histone H2A.2 OS=Pisum sativum PE=2 SV=1	[more]
H2A1_MEDTR	1.859e-9	96.67	Probable histone H2A.1 OS=Medicago truncatula GN=M...	[more]
H2A2_WHEAT	2.427e-9	93.33	Histone H2A.2.1 OS=Triticum aestivum PE=1 SV=1	[more]
H2A1_SOLLC	2.427e-9	93.33	Histone H2A.1 OS=Solanum lycopersicum PE=2 SV=1	[more]

BLAST of ES560253 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9T4D5_RICCO	1.333e-8	96.67	Histone H2A OS=Ricinus communis GN=RCOM_0390300 PE...	[more]
B9T4D3_RICCO	1.333e-8	96.67	Histone H2A OS=Ricinus communis GN=RCOM_0390180 PE...	[more]
C5XBR4_SORBI	2.971e-8	93.33	Histone H2A OS=Sorghum bicolor GN=Sb02g004970 PE=3...	[more]
B6U5W4_MAIZE	2.971e-8	93.33	Histone H2A OS=Zea mays PE=2 SV=1	[more]
B6T832_MAIZE	2.971e-8	93.33	Histone H2A OS=Zea mays PE=2 SV=1	[more]
B6T3E9_MAIZE	2.971e-8	93.33	Histone H2A OS=Zea mays PE=2 SV=1	[more]
B6T0P6_MAIZE	2.971e-8	93.33	Histone H2A OS=Zea mays PE=2 SV=1	[more]
B6SYW4_MAIZE	2.971e-8	93.33	Histone H2A OS=Zea mays PE=2 SV=1	[more]
B6SYU0_MAIZE	2.971e-8	93.33	Histone H2A OS=Zea mays PE=2 SV=1	[more]
B6SJU3_MAIZE	2.971e-8	93.33	Histone H2A OS=Zea mays PE=2 SV=1	[more]

BLAST of ES560253 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 7


Match Name	E-value	Identity	Description
AT5G02560.2	4.837e-9	80.00	\| Symbols: HTA12 \| histone H2A 12 \| chr5:575437-57...	[more]
AT5G02560.1	4.837e-9	80.00	\| Symbols: HTA12 \| histone H2A 12 \| chr5:575437-57...	[more]
AT5G59870.1	6.985e-8	76.67	\| Symbols: HTA6 \| histone H2A 6 \| chr5:24115605-24...	[more]
AT5G27670.1	9.123e-8	76.67	\| Symbols: HTA7 \| histone H2A 7 \| chr5:9792807-979...	[more]
AT1G51060.1	9.123e-8	79.31	\| Symbols: HTA10 \| histone H2A 10 \| chr1:18926948-...	[more]
AT1G08880.1	3.467e-7	73.33	\| Symbols: HTA5, H2AXA, G-H2AX, GAMMA-H2AX \| Histo...	[more]
AT1G54690.1	4.527e-7	73.33	\| Symbols: HTA3, H2AXB, G-H2AX, GAMMA-H2AX \| gamma...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002119	Histone H2A	PRINTS	PR00620	HISTONEH2A	coord: 27..49 score: 1.2e-10coord: 56..58 score: 1.2
IPR002119	Histone H2A	PANTHER	PTHR23430	HISTONE H2A	coord: 21..58 score: 5.6
IPR002119	Histone H2A	PROSITE	PS00046	HISTONE_H2A	coord: 35..41 score
IPR009072	Histone-fold	GENE3D	1.10.20.10	no description	coord: 18..58 score: 3.7
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 7..58 score: 7.9
None	No IPR available	SEG	seg	seg	coord: 8..28 score
None	No IPR available	SEG	seg	seg	coord: 31..48 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>ES560253 ID=ES560253; Name=ES560253; organism=Cicer arietinum; type=EST; length=244bp
CCATATTCCTCCTCACATTACAATCTACTCATCCTTCATTCCTCATAATC
TAACTTTGCACAAAATCTAGGGTTCCAATTTCACAATGGAAGCTAGCACA
AAAACGAAGAAAGGAGCCGGAGGAAGGAAAGGAGGAGGACCAAGGAAGAA
GTCGGTAACAAGATCTATCAGAGCCGGTCTTCAATTTCCCGTCGGTAGAA
TCGGCCGTTACTTGAAGAAAGGTAGATATGCTCAGCGTGTTGGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0000786	nucleosome
GO:0005634	nucleus

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006334	nucleosome assembly

Vocabulary: **INTERPRO**
Term	Definition
IPR002119	Histone_H2A
IPR009072	Histone-fold

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